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公开(公告)号：US20100279374A1
公开(公告)日：2010-11-04
申请号：US12761066
申请日：2010-04-15
CPC分类号： G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnetic field. Other embodiments are also provided.
摘要翻译： 本发明提供了一种在液滴致动器中的液滴内分散或循环磁响应珠的方法。 在一个实施例中，本发明利用具有配置成传送液滴的多个液滴操作电极的液滴致动器和存在于多个液滴操作电极的一部分处的磁场。 在均匀磁场存在的情况下，在液滴致动器上提供含珠的液滴。 在孵育期间，通过在磁场的均匀区域内的液滴上进行液滴操作，珠子在液滴中循环。 还提供了其他实施例。
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公开(公告)号：US20100068764A1
公开(公告)日：2010-03-18
申请号：US12524495
申请日：2008-02-11
申请人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt
发明人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt
CPC分类号： G01N33/54326 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L2200/025 , B01L2200/028 , B01L2200/0647 , B01L2200/0673 , B01L2200/141 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0427 , B01L2400/043 , C12M25/01 , C12M25/16 , G01N27/44717 , G01N27/44786 , G01N33/54386 , G01N35/0098 , G01N2035/1046
摘要： A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator (200) comprising: (i) a substrate (210) comprising electrodes (214) arranged for conducting droplet operations on a surface; (ii) a starting droplet (218/222) comprising: (1) one or more magnetically responsive beads (220); (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone, (224); (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet (218) comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet (222) substantially lacking the magnetically responsive beads.
摘要翻译： 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤：（a）提供液滴致动器（200），包括：（i）包括布置用于在表面上进行液滴操作的电极（214）的基底（210） （ii）起始液滴（218/222），包括：（1）一个或多个磁响应珠（220）; （2）物质的起始量; 和（3）起始体积; （b）将所述一个或多个磁响应珠磁性地固定在距离目标液滴分离区一段距离的位置处（224）; （c）进行一个或多个液滴操作，其包括液滴分割操作，所述液滴分割操作被选择以分割组合的液滴以产生一组液滴，其包括：（i）液滴（218），其基本上包含所有一个或多个磁响应珠粒， 物质的数量相对于起始浓度; 和（ii）基本上不含磁响应珠的液滴（222）。
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公开(公告)号：US20130264010A1
公开(公告)日：2013-10-10
申请号：US13618322
申请日：2012-09-14
CPC分类号： H05K3/4038 , B01L3/502707 , B01L3/502784 , B01L3/502792 , B01L2300/089 , B01L2300/12 , B01L2400/0427 , H05K1/113 , H05K3/0058 , H05K3/0067 , H05K3/28 , H05K3/421 , H05K3/427 , H05K2201/09527 , Y10T29/49124 , Y10T29/49155 , Y10T29/49165 , Y10T156/10
摘要： Alternative approaches to fabricating printed circuit boards for use in droplet actuator operations are provided. In one embodiment, a method of manufacturing a droplet actuator for conducting droplet operations includes positioning a dielectric material between a first metal layer configured to include an electrode and a second metal layer configured to include an interconnect pad. The method additionally includes forming a connection between the first and second metal layers. Droplet actuators and methods of fabricating and supporting printed circuit boards of droplet actuators are also provided.
摘要翻译： 提供制造用于液滴致动器操作的印刷电路板的替代方法。 在一个实施例中，制造用于进行液滴操作的液滴致动器的方法包括将介电材料定位在被配置为包括电极的第一金属层和被配置为包括互连焊盘的第二金属层之间。 该方法还包括在第一和第二金属层之间形成连接。 还提供了液滴致动器和制造和支持液滴致动器的印刷电路板的方法。
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公开(公告)号：US08460528B2
公开(公告)日：2013-06-11
申请号：US12681879
申请日：2008-10-14
IPC分类号： G01N27/26
CPC分类号： B01L3/5027 , B01J2219/00371 , B01J2219/00378 , B01J2219/00385 , B01J2219/00515 , B01J2219/00518 , B01J2219/0065 , B01J2219/00653 , B01L3/502715 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/0668 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/0845 , B01L2300/089 , B01L2400/0427 , B01L2400/0478 , B01L2400/0487
摘要： On-board reagent storage, handling, and dispensing apparatuses and methods for droplet actuator systems are provided. In one embodiment, a fluidic analyzer is provided and includes a droplet actuator including a substrate including a plurality of electrodes configured to actuate a droplet and a reagent storage component in selective fluid communication with the droplet actuator and including a reagent configured to combine with a solvent at a time of use. In another embodiment, a method of conducting fluidic analysis is provided and includes storing a reagent within a reagent storage component in fluid communication with a droplet actuator including a plurality of electrodes configured to actuate a droplet and combining the reagent with a solvent at a time for use within the droplet actuator.
摘要翻译： 提供了用于液滴致动器系统的机载试剂储存，处理和分配装置和方法。 在一个实施例中，提供了一种流体分析器，其包括液滴致动器，该液滴致动器包括基底，该基底包括配置成致动液滴的多个电极和与液滴致动器选择性流体连通的试剂储存部件，并且包括配置成与溶剂组合的试剂 在使用时。 在另一个实施例中，提供了一种进行流体分析的方法，包括将试剂储存在与液滴致动器流体连通的液体致动器内，所述液滴致动器包括配置成致动液滴的多个电极并且一次将溶剂与溶剂组合 在液滴致动器内使用。
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公开(公告)号：US08268246B2
公开(公告)日：2012-09-18
申请号：US12531794
申请日：2008-08-11
CPC分类号： H05K3/4038 , B01L3/502707 , B01L3/502784 , B01L3/502792 , B01L2300/089 , B01L2300/12 , B01L2400/0427 , H05K1/113 , H05K3/0058 , H05K3/0067 , H05K3/28 , H05K3/421 , H05K3/427 , H05K2201/09527 , Y10T29/49124 , Y10T29/49155 , Y10T29/49165 , Y10T156/10
摘要： Alternative approaches to fabricating printed circuit boards for use in droplet actuator operations are provided. In one embodiment, a method of manufacturing a droplet actuator for conducting droplet operations includes positioning a dielectric material between a first metal layer configured to include an electrode and a second metal layer configured to include an interconnect pad. The method additionally includes forming a connection between the first and second metal layers. Droplet actuators and methods of fabricating and supporting printed circuit boards of droplet actuators are also provided.
摘要翻译： 提供制造用于液滴致动器操作的印刷电路板的替代方法。 在一个实施例中，制造用于进行液滴操作的液滴致动器的方法包括将介电材料定位在被配置为包括电极的第一金属层和被配置为包括互连焊盘的第二金属层之间。 该方法还包括在第一和第二金属层之间形成连接。 还提供了液滴致动器和制造和支持液滴致动器的印刷电路板的方法。
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公开(公告)号：US20110303542A1
公开(公告)日：2011-12-15
申请号：US12672564
申请日：2008-08-08
IPC分类号： G01N27/447 , B01D57/02
CPC分类号： B01L3/502792 , B01L2200/141 , B01L2300/0645 , B01L2400/0415 , G01N2035/00277
摘要： The invention relates to a droplet actuator with a substrate comprising electrodes arranged for conducting droplet operations on a droplet operations surface of the substrate; a filler fluid phase in contact with the droplet operations surface at least partially surrounding a droplet phase comprising a droplet arranged on one or more of the electrodes, the droplet comprising: (i) a target substance susceptible to loss from the droplet phase into the filler fluid phase; and (ii) an additive which reduces loss of the target substance to the filler fluid phase relative to a corresponding droplet not comprising the additive. The invention also relates to various compositions and methods.
摘要翻译： 本发明涉及一种液滴致动器，其具有包括布置用于在基板的液滴操作表面上进行液滴操作的电极的基板; 与液滴操作表面接触的填充流体相，至少部分地围绕包含布置在一个或多个电极上的液滴的液滴相，所述液滴包括：（i）易受从液滴相流入填料中的目标物质 流体相; 和（ii）相对于不包含添加剂的相应液滴，减少目标物质对填料流体相的损失的添加剂。 本发明还涉及各种组合物和方法。
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公开(公告)号：US20110256542A9
公开(公告)日：2011-10-20
申请号：US12546115
申请日：2009-08-24
申请人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
发明人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
IPC分类号： C12Q1/68
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，液滴致动器用于使用聚合酶链反应（PCR）技术进行遗传分析。 本发明解决了对提供样品靶的最佳放大和检测的在液滴致动器上进行PCR的改进方法的需要。
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公开(公告)号：US20100282609A1
公开(公告)日：2010-11-11
申请号：US12681879
申请日：2008-10-14
IPC分类号： B01J19/08
CPC分类号： B01L3/5027 , B01J2219/00371 , B01J2219/00378 , B01J2219/00385 , B01J2219/00515 , B01J2219/00518 , B01J2219/0065 , B01J2219/00653 , B01L3/502715 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/0668 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/0845 , B01L2300/089 , B01L2400/0427 , B01L2400/0478 , B01L2400/0487
摘要： On-board reagent storage, handling, and dispensing apparatuses and methods for droplet actuator systems are provided. In one embodiment, a fluidic analyzer is provided and includes a droplet actuator including a substrate including a plurality of electrodes configured to actuate a droplet and a reagent storage component in selective fluid communication with the droplet actuator and including a reagent configured to combine with a solvent at a time of use. In another embodiment, a method of conducting fluidic analysis is provided and includes storing a reagent within a reagent storage component in fluid communication with a droplet actuator including a plurality of electrodes configured to actuate a droplet and combining the reagent with a solvent at a time for use within the droplet actuator.
摘要翻译： 提供了用于液滴致动器系统的机载试剂储存，处理和分配装置和方法。 在一个实施例中，提供了一种流体分析器，其包括液滴致动器，该液滴致动器包括基底，该基底包括配置成致动液滴的多个电极和与液滴致动器选择性流体连通的试剂储存部件，并且包括配置成与溶剂组合的试剂 在使用时。 在另一个实施例中，提供了一种进行流体分析的方法，包括将试剂储存在与液滴致动器流体连通的液体致动器内，所述液滴致动器包括配置成致动液滴的多个电极并且一次将溶剂与溶剂组合 在液滴致动器内使用。
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公开(公告)号：US20100258441A1
公开(公告)日：2010-10-14
申请号：US12761147
申请日：2010-04-15
CPC分类号： B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502746 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N35/0098 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
摘要翻译： 本发明提供了一种在液滴致动器中的液滴内循环磁响应珠的方法。 本发明还提供了分裂液滴的方法。 在一个实施例中，本发明利用具有顶部和底部基底的液滴致动器，分别存在于顶部和底部基底附近的多个磁场，其中至少一个磁场可选择性地改变，并且多个液滴 沿着顶表面和底表面中的至少一个定位的操作电极。 液滴位于顶表面和底表面之间，并且至少一个磁场被选择性地改变。
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公开(公告)号：US20100236928A1
公开(公告)日：2010-09-23
申请号：US12681848
申请日：2008-10-15
CPC分类号： B01L3/502792 , B01L3/502761 , B01L2200/0673 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/088 , B01L2300/089 , B01L2400/0424 , B01L2400/0427 , B01L2400/0448
摘要： A droplet actualor having electrodes configured for ejecting droplet operations transporting droplets on a surface, and a sensor arranged in proximity to one or more of the electrodes establishing a detection window on the surface for detection of one or more properties of one or more droplets on the surface, wherein the electrodes establish at least two pathways for transport of droplets into the detection window. Also provided is, A method of detecting a property of a target droplet, including using droplet operations to modulate signals from a droplet set comprising the target droplet, detecting the modulated signals of the droplet set, demodulating the modulated signals to identify the signal produced by one or more individual droplets of the set Related methods and alternative embodiments are also provided.
摘要翻译： 具有配置用于喷射在表面上输送液滴的液滴操作的电极的液滴实体，以及布置在一个或多个电极附近的传感器，其在表面上建立检测窗口，用于检测表面上的一个或多个液滴的一个或多个特性 表面，其中电极建立用于将液滴输送到检测窗中的至少两个路径。 还提供了一种检测目标液滴的属性的方法，包括使用液滴操作来调制来自包括目标液滴的液滴组的信号，检测液滴组的调制信号，解调调制信号以识别由 还提供了一组或多个组合的相关方法和替代实施例。
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