公开(公告)号：US20190144482A1

公开(公告)日：2019-05-16

申请号：US16094845

申请日：2017-04-21

Applicant: Complete Genomics, Inc. ,  BGI Shenzhen

Inventor： Handong Li ,  Snezana Drmanac ,  Radoje Drmanac ,  Xun Xu ,  Lingling Peng ,  Scott Gablenz

IPC: C07H15/04 ,  C07H19/10 ,  C07H21/04 ,  C07H19/20 ,  C12Q1/6869

Abstract: Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.

公开(公告)号：US20250066748A1

公开(公告)日：2025-02-27

申请号：US18723633

申请日：2021-12-31

Applicant: BGI Shenzhen

Inventor： Lele Wang ,  Tao Zeng ,  Zhenjun Liu ,  Junyi Chen ,  Ziyu Zhao ,  Denghui Li ,  Fei Guo ,  Zhouxiang Ji ,  Ou Wang ,  Yuxiang Li ,  Yuliang Dong ,  Wenwei Zhang ,  Xun Xu

IPC: C12N9/14 ,  C12N15/70 ,  C12Q1/34 ,  C12Q1/6869

Abstract: The present invention provides a helicase BCH2X, comprising an amino acid sequence represented by any one of SEQ ID NOs: 1-3. The present invention also provides a complex structure comprising the helicase BCH2X and a binding moiety for binding polynucleotides. The present invention also provides a use of the helicase BCH2X or the complex structure comprising same in the control and characterization of polynucleotides and single-molecule nanopore sequencing.

公开(公告)号：US12195493B2

公开(公告)日：2025-01-14

申请号：US17208831

申请日：2021-03-22

Applicant: MGI Tech Co., Ltd. ,  BGI Shenzhen

Inventor： Handong Li ,  Snezana Drmanac ,  Radoje Drmanac ,  Xun Xu ,  Lingling Peng ,  Scott Gablenz

IPC: C07H15/04 ,  C07H19/10 ,  C07H19/20 ,  C07H21/04 ,  C12Q1/6869

Abstract: Reversibly blocked nucleoside analogues and methods of using such nucleoside analogues for sequencing of nucleic acids are provided.

公开(公告)号：US11636340B2

公开(公告)日：2023-04-25

申请号：US16967087

申请日：2018-04-17

Applicant: BGI SHENZHEN ,  EYE, EAR, NOSE, AND THROAT HOSPITAL OF FUDAN UNIVERSITY

Inventor： Xiaoqing Liu ,  Jiaxu Hong ,  Yong Ni ,  Shuangshuang Li ,  Lili Wang ,  Wei He ,  Youwen Guo ,  Yuxuan Liu ,  Yong Liu ,  Wei Wang ,  Ruiqi Xu ,  Jingyi Cheng ,  Lijia Tian ,  Wenbin Chen ,  Xun Xu

IPC: G06N3/08 ,  G16H50/20 ,  G16H10/20 ,  A61B3/12 ,  A61B3/14 ,  G06T7/00 ,  G06F18/214 ,  G06F18/21 ,  G06V10/764 ,  G06V10/80 ,  G06V10/82 ,  G06V20/69

Abstract: The present disclosure proposes a modeling method and apparatus for diagnosing an ophthalmic disease based on artificial intelligence, and a storage medium. The modeling method includes: establishing a data collection of ophthalmic images and a data collection of non-image ophthalmic disease diagnosis questionnaires; training a first neural network model by employing the data collection of the ophthalmic images to obtain a first classification model; training a second classification model by employing the data collection of non-image ophthalmic disease diagnosis questionnaires; and merging the first classification model and the second classification model to obtain a target classification network model, in which, a test result outputted by the target classification network model is used as a diagnosis result of the ophthalmic disease.

公开(公告)号：US10179934B2

公开(公告)日：2019-01-15

申请号：US15643078

申请日：2017-07-06

Applicant: BGI Shenzhen

Inventor： Kang Kang ,  Shihong Chen ,  Yue Shen ,  Yun Wang ,  Xun Xu

IPC: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12N15/00

Abstract: Disclosed is a high-throughput detection method for a DNA synthesis product. According to the method of the present invention, a high-throughput sequencing technology is applied to detection of a DNA chip synthesis product through a mixed library construction strategy, so that costs of product detection and accurate product screening are reduced.

公开(公告)号：US20180223358A1

公开(公告)日：2018-08-09

申请号：US15862566

申请日：2018-01-04

Applicant: Complete Genomics, Inc. ,  BGI Shenzhen

Inventor： Radoje Drmanac ,  Snezana Drmanac ,  Handong Li ,  Xun Xu ,  Matthew J. Callow ,  Leon Eckhardt ,  Naibo Yang ,  Quan Ding

IPC: C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  A61K47/00 ,  C07K16/2851 ,  C07K16/44 ,  C07K2317/73 ,  C12Q1/6804 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q2525/186

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

公开(公告)号：US12276809B2

公开(公告)日：2025-04-15

申请号：US17769428

申请日：2020-03-09

Applicant: BGI SHENZHEN

Inventor： Jielei Ni ,  Ming Ni ,  Fan Zhou ,  Zeyu Su ,  Ke Ji ,  Dong Wei ,  Mengzhe Shen ,  Yuanqing Liang ,  Mei Li ,  Xun Xu

IPC: G01N21/00 ,  C12Q1/6869 ,  G01N21/64 ,  G02B21/14 ,  G02B21/16 ,  G02B21/36 ,  G02B27/28 ,  G02B27/58

Abstract: Disclosed are a super-resolution imaging system (1, 41, 51), a super-resolution imaging method, a biological sample identification system (4, 61) and method, a nucleic acid sequencing imaging system (5) and method, and a nucleic acid identification system (6) and method. The super-resolution imaging system (1, 41, 51) includes an illumination system (A) and an imaging system (B). The illumination system (A) outputs excitation light to irradiate a biological sample to generate excited light, and the imaging system (B) collects and records the excited light to generate an excited light image. The illumination system (A) includes an excitation light source (10, 10a) and a structured light generation and modulation device (11, 11a). The excitation light source (10, 10a) outputs the excitation light, and the structured light generation and modulation device (11, 11a) modulates the excitation light into structured light to irradiate the biological sample to generate the excited light.

公开(公告)号：US20240392263A1

公开(公告)日：2024-11-28

申请号：US18032755

申请日：2020-10-21

Applicant: BGI SHENZHEN

Inventor： Lili Zhai ,  Qingqing Xie ,  Ruyin Cao ,  Zi Wang ,  Yue Zheng ,  Yuliang Dong ,  Wenwei Zhang ,  Xun Xu

IPC: C12N9/12 ,  C12Q1/6869

Abstract: Provided is a recombinant KOD polymerase. A KOD polymerase mutant is a protein as follows: the protein is a protein having DNA polymerase activity that is obtained by modifying amino acid residues in at least one of the following 48 positions in the amino acid sequence of KOD DNA polymerase GH78: 267, 326, 347, 349, 353, 375, 378, 379, 380, 385, 451, 452, 453, 454, 457, 461, 465, 470, 474, 477, 478, 479, 480, 482, 484, 485, 486, 493, 496, 497, 514, 574, 584, 605, 610, 630, 665, 666, 667, 674, 676, 680, 682, 698, 707, 718, 723 and 729, without changing other amino acid sequences; and compared with KOD DNA polymerase GH78, with regard to catalysis, the recombinant DNA polymerase exhibits a faster reaction rate, better catalytic efficiency, better affinity and other advantages, thereby improving the reaction rate of DNA polymerase in sequencing and increasing the reaction read length.

公开(公告)号：US20240281625A1

公开(公告)日：2024-08-22

申请号：US18651099

申请日：2024-04-30

Applicant: BGI SHENZHEN

Inventor： Wenwei Zhang ,  Mei Li ,  Weimao Wang ,  Yuxiang Li ,  Xun Xu

IPC: G06K7/08 ,  G01N33/543 ,  G06T5/77 ,  G06T7/00 ,  G06T7/13 ,  G06T7/62 ,  G06T7/73

CPC classification number: G06K7/087 ,  G01N33/54326 ,  G06T5/77 ,  G06T7/0002 ,  G06T7/13 ,  G06T7/62 ,  G06T7/73 ,  G06T2207/10024 ,  G06T2207/10064 ,  G06T2207/20081 ,  G06T2207/20084 ,  G06T2207/30204

Abstract: Provided are a magnetic bead-based detection method, a storage medium, and a detection device. The detection method includes: collecting a white light image of a to-be-detected solution, in which the to-be-detected solution is mixed with a to-be-detected sample and magnetic beads with a capture agent (S1); determining magnetic stripe regions in the white light image, and determining first magnetic bead regions based on the magnetic stripe regions (S2); selecting, by using a first neural network, second magnetic bead regions containing magnetic beads from the first magnetic bead regions, and obtaining a marker position of each of the magnetic beads (S3); and obtaining, by using a second neural network and based on each of the second magnetic bead regions, codes at code bits of a corresponding magnetic bead, and obtaining corresponding code information based on the codes of the code bits and the marker position of the magnetic bead (S4).

公开(公告)号：US20210254150A1

公开(公告)日：2021-08-19

申请号：US17084482

申请日：2020-10-29

Applicant: MGI Tech Co., Ltd. ,  BGI Shenzhen

Inventor： Radoje Drmanac ,  Snezana Drmanac ,  Handong Li ,  Xun Xu ,  Matthew J. Callow ,  Leon Eckhardt ,  Naibo Yang

IPC: C12Q1/6874 ,  C12Q1/6876 ,  C07K16/44 ,  C12Q1/6804 ,  A61K47/00 ,  C12Q1/6869 ,  C07K16/28

Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.