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公开(公告)号：US20200064337A1
公开(公告)日：2020-02-27
申请号：US16391063
申请日：2019-04-22
Applicant: Berkeley Lights, Inc.
Inventor： Minha Park , Jason C. Briggs , Jason M. McEwen , Ravi K. Ramenani , Hariharasudhan Chirra Dinakar , Kai W. Szeto , Adrienne T. Higa , Mark P. White , Randall D. Lowe, JR. , Xiaohua Wang , Kevin T. Chapman
IPC: G01N33/50 , B01L3/00 , C12N5/0781 , G01N33/68 , C12Q1/6876
Abstract: Methods are described herein for screening an antibody producing cell within a microfluidic environment. The antibody producing cell may be a B cell lymphocyte, which may be a memory B cell or a plasma cell. An antigen of interest may be brought into proximity with the antibody producing cell and binding of the antigen by an antibody produced by the antibody producing cell may be monitored. Methods of obtaining a sequencing library from an antibody producing cell are also described.
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公开(公告)号：US20190283026A1
公开(公告)日：2019-09-19
申请号：US16253869
申请日：2019-01-22
Applicant: Berkeley Lights, Inc.
Inventor： Kevin D. Loutherback , Yelena Bronevetsky , Peter J. Beemiller , Xiaohua Wang , Kevin T. Chapman
Abstract: Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (Dc) that separates activated T lymphocytes from naïve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from naïve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.
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公开(公告)号：US10376886B2
公开(公告)日：2019-08-13
申请号：US15843122
申请日：2017-12-15
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M. Jimena Loureiro
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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公开(公告)号：US20180259482A1
公开(公告)日：2018-09-13
申请号：US15842124
申请日：2017-12-14
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Igor Y. Khandros , Gaetan L. Mathieu , J. Tanner Nevill , Ming C. Wu
IPC: G01N27/447 , B01L3/00 , B03C5/00 , B03C5/02
Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.
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公开(公告)号：US09889445B2
公开(公告)日：2018-02-13
申请号：US14521447
申请日：2014-10-22
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M Jimena Loureiro
CPC classification number: B01L3/502761 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0454 , B03C5/005 , B03C5/026 , B03C2201/26 , G01N15/1484 , G01N33/5023 , G01N33/5047 , G01N33/505 , G01N33/5052 , G01N33/54313 , G01N33/6854
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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Patent Agency Ranking
公开(公告)号：US20150165436A1
公开(公告)日：2015-06-18
申请号：US14521447
申请日：2014-10-22
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White
CPC classification number: B01L3/502761 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0454 , B03C5/005 , B03C5/026 , B03C2201/26 , G01N15/1484 , G01N33/5023 , G01N33/5047 , G01N33/505 , G01N33/5052 , G01N33/54313 , G01N33/6854
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
Abstract translation: 在微流体装置中保持笔的生物活性可以通过放置在保持笔中捕获物体来测定，所述对象捕获物质与生物活性产生的特定感兴趣物质结合。 然后可以在微流体装置中或在从微流体装置输出捕获物体之后评估与每个捕获物体结合的感兴趣的生物材料。 该评估可用于表征每支持笔中的生物活性。 生物活性可以是生产感兴趣的生物材料。 因此，生物活性可以对应于或来自一个或多个生物细胞。 持有笔内的生物细胞可以是克隆细胞集落。 可以在保持每个菌落的克隆状态的同时测定每个克隆细胞集落的生物学活性。
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公开(公告)号：US20140120558A1
公开(公告)日：2014-05-01
申请号：US14044559
申请日：2013-10-02
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman
IPC: G01N33/569
CPC classification number: G01N33/56972 , G01N33/56911 , G01N33/56961 , G01N33/56966 , G01N33/6854 , G01N2458/00 , G01N2469/10
Abstract: In some cases, the described systems and methods include obtaining a cell sample containing multiple antibody-producing cells. In such cases, the cells can be tagged with a cross-linking reagent having a first portion configured to bind to a marker on the antibody-producing cells and a second portion configured to bind to an antigen of interest. In some instances, the tagged antibody-producing cells are exposed to the antigen of interest such that the antigen becomes bound to the cells. In some such instances, the antibody-producing cells are also allowed to produce an antibody, such that a portion of the antibody-producing cells produce an antigen-specific antibody that binds to the antigen of interest. To identify cells that produce the antigen-specific antibody, the tagged cells can be exposed to a labeled secondary antibody that is configured to bind to the antigen-specific antibody. Other implementations are also described.
Abstract translation: 在一些情况下，所描述的系统和方法包括获得含有多个产生抗体的细胞的细胞样品。 在这种情况下，可以用交联试剂对细胞进行标记，所述交联试剂具有构建成结合抗体产生细胞上的标记的第一部分和被配置成与感兴趣的抗原结合的第二部分。 在一些情况下，标记的产生抗体的细胞暴露于感兴趣的抗原，使得抗原与细胞结合。 在一些这样的情况下，也允许产生抗体的细胞产生抗体，使得产生一部分抗体的细胞产生与感兴趣的抗原结合的抗原特异性抗体。 为了鉴定产生抗原特异性抗体的细胞，标记的细胞可以暴露于被配置为结合抗原特异性抗体的标记的二抗。 还描述了其他实现。
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公开(公告)号：US11998914B2
公开(公告)日：2024-06-04
申请号：US17656244
申请日：2022-03-24
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M. Jimena Loureiro
CPC classification number: B01L3/502761 , B03C5/005 , B03C5/026 , G01N15/1484 , G01N33/5023 , G01N33/5047 , G01N33/505 , G01N33/5052 , G01N33/54313 , G01N33/6854 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0454 , B03C2201/26
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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公开(公告)号：US11666912B2
公开(公告)日：2023-06-06
申请号：US16253869
申请日：2019-01-22
Applicant: Berkeley Lights, Inc.
Inventor： Kevin D. Loutherback , Yelena Bronevetsky , Peter J. Beemiller , Xiaohua Wang , Kevin T. Chapman
CPC classification number: B01L3/502746 , A61K35/17 , A61K35/28 , B01L3/502761 , B01L3/502792 , B03C5/005 , B03C5/026 , G01N1/34 , B01L3/502784 , B01L2200/0652 , B01L2300/0645 , B01L2300/0816 , B01L2300/12 , B01L2300/165 , B01L2400/0424 , B01L2400/086 , B03C2201/26 , G01N2001/4088
Abstract: Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (Dc) that separates activated T lymphocytes from naïve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from naïve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.
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公开(公告)号：US10973227B2
公开(公告)日：2021-04-13
申请号：US15136777
申请日：2016-04-22
Applicant: BERKELEY LIGHTS, INC.
Inventor： Mark P. White , Kevin T. Chapman , Andrew W. McFarland , Eric D. Hobbs , Randall D. Lowe, Jr.
Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.
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