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公开(公告)号:US11376591B2
公开(公告)日:2022-07-05
申请号:US16893885
申请日:2020-06-05
Applicant: Berkeley Lights, Inc.
Inventor: Troy A. Lionberger , Brandon R. Bruhn , John A. Tenney , Eric D. Hobbs
IPC: B01L3/00 , G01N27/447 , B03C5/00 , B03C5/02
Abstract: Optically-actuated microfluidic devices permit the use of spatially-modulated light to manipulate micro-objects such as biological cells. Systems and methods are described for providing sequences of light patterns to move and direct a plurality of micro-objects within the environment of a microfluidic device. The sequenced light patterns provide improved efficiency in directing the transport of the plurality of micro-objects. Other embodiments are described.
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公开(公告)号:US11203018B2
公开(公告)日:2021-12-21
申请号:US16160816
申请日:2018-10-15
Applicant: Berkeley Lights, Inc.
Inventor: Troy A. Lionberger , Matthew E. Fowler , Phillip J. M. Elms , Kevin D. Loutherback , Randall D. Lowe, Jr. , Jian Gong , J. Tanner Nevill , Gang F. Wang , Gregory G. Lavieu , John A. Tenney , Aathavan Karunakaran , Anupam Singhal , I-Jong Lin
Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.
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公开(公告)号:US10675625B2
公开(公告)日:2020-06-09
申请号:US15487795
申请日:2017-04-14
Applicant: Berkeley Lights, Inc.
Inventor: Troy A. Lionberger , Brandon R. Bruhn , John A. Tenney , Eric D. Hobbs
IPC: B01L3/00 , G01N27/447 , B03C5/00 , B03C5/02
Abstract: Optically-actuated microfluidic devices permit the use of spatially-modulated light to manipulate micro-objects such as biological cells. Systems and methods are described for providing sequences of light patterns to move and direct a plurality of micro-objects within the environment of a microfluidic device. The sequenced light patterns provide improved efficiency in directing the transport of the plurality of micro-objects. Other embodiments are described.
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公开(公告)号:US20190172196A1
公开(公告)日:2019-06-06
申请号:US16000385
申请日:2018-06-05
Applicant: Berkeley Lights, Inc.
Inventor: Fenglei Du , Paul M. Lundquist , John A. Tenney , Troy A. Lionberger
IPC: G06T7/00 , G01N21/64 , G01N15/14 , G06T5/50 , G01N27/453
CPC classification number: G06T7/0012 , B01L3/502761 , G01N15/1434 , G01N15/1463 , G01N15/1475 , G01N15/1484 , G01N21/6456 , G01N27/453 , G01N2015/0038 , G01N2015/0065 , G01N2015/1006 , G01N2015/1445 , G01N2015/1486 , G01N2015/1493 , G01N2015/1497 , G01N2021/056 , G01N2021/1765 , G01N2201/0635 , G01N2201/127 , G06T5/50 , G06T2207/30024 , G06T2207/30101
Abstract: Methods are provided for the automated detection of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.
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公开(公告)号:US09996920B2
公开(公告)日:2018-06-12
申请号:US14963230
申请日:2015-12-08
Applicant: Berkeley Lights, Inc.
Inventor: Fenglei Du , Paul M. Lundquist , John A. Tenney , Troy A. Lionberger
IPC: G06T7/00 , G01N15/14 , G06T5/50 , G01N27/453 , G01N21/64 , G01N15/00 , G01N15/10 , G01N21/05 , G01N21/17 , B01L3/00
CPC classification number: G06T7/0012 , B01L3/502761 , G01N15/1434 , G01N15/1463 , G01N15/1475 , G01N15/1484 , G01N21/6456 , G01N27/453 , G01N2015/0038 , G01N2015/0065 , G01N2015/1006 , G01N2015/1445 , G01N2015/1486 , G01N2015/1493 , G01N2015/1497 , G01N2021/056 , G01N2021/1765 , G01N2201/0635 , G01N2201/127 , G06T5/50 , G06T2207/30024 , G06T2207/30101
Abstract: Methods are provided for the automated detection of micro-objects in a microfluidic device. In addition, methods are provided for repositioning micro-objects in a microfluidic device. In addition, methods are provided for separating micro-objects in a spatial region of the microfluidic device.
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16.
公开(公告)号:US11802264B2
公开(公告)日:2023-10-31
申请号:US17067971
申请日:2020-10-12
Applicant: Berkeley Lights, Inc.
Inventor: Volker L. S. Kurz , Troy A. Lionberger , Eric K. Sackmann , Kai W. Szeto , Paul M. Lebel , Brandon R. Bruhn , Keith J. Breinlinger , Eric D. Hobbs , Andrew W. McFarland , J. Tanner Nevill , Xiaohua Wang
CPC classification number: C12M23/16 , B01L3/502761 , C12M23/20 , C12M33/12 , B01L2200/0668 , B01L2300/1822 , B01L2400/0415 , B01L2400/0427 , B01L2400/0442 , B01L2400/0454 , B01L2400/086
Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.
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公开(公告)号:US11666913B2
公开(公告)日:2023-06-06
申请号:US15359115
申请日:2016-11-22
Applicant: Berkeley Lights, Inc.
Inventor: Kristin G. Beaumont , Nan-Linda Ding , Volker L. S. Kurz , Troy A. Lionberger , Randall D. Lowe, Jr. , Daniele Malleo , Andrew W. McFarland , J. Tanner Nevill , Xiaohua Wang
IPC: B01L3/00 , B01J19/00 , G01N27/447
CPC classification number: B01L3/502753 , B01J19/0093 , B01L3/5023 , B01L3/502707 , B01L3/502738 , B01L3/502761 , G01N27/44791 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/16 , B01L2400/0424 , B01L2400/0677 , B01L2400/08
Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.
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18.
公开(公告)号:US20210102150A1
公开(公告)日:2021-04-08
申请号:US17067971
申请日:2020-10-12
Applicant: Berkeley Lights, Inc.
Inventor: Volker L.S. Kurz , Troy A. Lionberger , Eric K. Sackmann , Kai W. Szeto , Paul M. Lebel , Brandon R. Bruhn , Keith J. Breinlinger , Eric D. Hobbs , Andrew W. McFarland , J. Tanner Nevill , Xiaohua Wang
Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.
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19.
公开(公告)号:US20180298318A1
公开(公告)日:2018-10-18
申请号:US16010176
申请日:2018-06-15
Applicant: Berkeley Lights, Inc.
Inventor: Volker L.S. Kurz , Troy A. Lionberger , Erik K. Sackmann , Kai W. Szeto , Paul M. Lebel , Brandon R. Bruhn , Keith J. Breinlinger , Eric D. Hobbs , Andrew W. McFarland , J. Tanner Nevill , Xiaohua Wang
CPC classification number: C12M23/16 , B01L3/502761 , B01L2200/0668 , B01L2300/1822 , B01L2400/0415 , B01L2400/0427 , B01L2400/0442 , B01L2400/0454 , B01L2400/086 , C12M23/20 , C12M33/12
Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.
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公开(公告)号:US20170165667A1
公开(公告)日:2017-06-15
申请号:US15359115
申请日:2016-11-22
Applicant: Berkeley Lights, Inc.
Inventor: Kristin G. Beaumont , Nan-Linda Ding , Volker L.S. Kurz , Troy A. Lionberger , Randall D. Lowe , Daniele Malleo , Andrew W. McFarland , J. Tanner Nevill , Xiaohua Wang
IPC: B01L3/00 , B01J19/00 , G01N27/447
CPC classification number: B01L3/502753 , B01J19/0093 , B01L3/5023 , B01L3/502707 , B01L3/502738 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/16 , B01L2400/0424 , B01L2400/0677 , B01L2400/08 , G01N27/44791
Abstract: In situ-generated microfluidic isolation structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. The ability to introduce in real time, a variety of isolating structures including pens and barriers offers improved methods of micro-object manipulation in microfluidic devices. The in situ-generated isolation structures may be permanently or temporarily installed.
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