公开(公告)号：US20130288920A1

公开(公告)日：2013-10-31

申请号：US13927887

申请日：2013-06-26

Applicant: BioArray Solutions, Ltd.

Inventor： Sukanta Banerjee ,  Michael Seul ,  Alice X. Li

IPC: C12Q1/68

CPC classification number: C12Q1/6813 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00315 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00545 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12Q1/6837 ,  C12Q1/6876 ,  G01N27/745 ,  G01N33/5434 ,  G01N33/54346 ,  G01N33/587 ,  G01N2035/00564 ,  G01N2035/00762 ,  G01N2446/20 ,  G01N2446/64 ,  Y10T436/11 ,  C12Q2565/513 ,  C12Q2563/143 ,  C12Q2563/107 ,  C12Q2563/155

Abstract: The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interations of a number of analyte molecules in a sample.

公开(公告)号：US10415081B2

公开(公告)日：2019-09-17

申请号：US15228377

申请日：2016-08-04

Applicant: BioArray Solutions, Ltd.

Inventor： Alice Xiang Li ,  Ghazala Hashmi ,  Michael Seul

IPC: C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6858 ,  C12Q1/6883

Abstract: The invention provides methods and processes for the identification of polymorphisms at one ore more designated sites, without interference from non-designated sites located within proximity of such designated sites. Probes are provided capable of interrogation of such designated sites in order to determine the composition of each such designated site. By the methods of this invention, one ore more mutations within the CFTR gene and the HLA gene complex can be identified.

公开(公告)号：US10138511B2

公开(公告)日：2018-11-27

申请号：US15472555

申请日：2017-03-29

Applicant: BioArray Solutions, Ltd.

Inventor： Michael Seul ,  Chiu Wo Chau ,  Hui Huang ,  Sukanta Banerjee ,  Jiacheng Yang ,  Ye Hong

IPC: C12Q1/6837 ,  B01J19/00 ,  G01N33/543

Abstract: This invention provides high unit density arrays of microparticles and methods of assembling such arrays. The microparticles in the arrays may be functionalized with chemical or biological entities specific to a given target analyte. The high unit density arrays of this invention are formed on chips which may be combined to form multichip arrays according to the methods described herein. The chips and/or multichip arrays of this invention are useful for chemical and biological assays.

公开(公告)号：US20170088885A9

公开(公告)日：2017-03-30

申请号：US14257294

申请日：2014-04-21

Applicant: BIOARRAY SOLUTIONS, LTD.

Inventor： Michael Seul ,  Sukanta Banerjee ,  Jiacheng Yang ,  Tatiana Vener

IPC: C12Q1/68

CPC classification number: C12Q1/6832 ,  B01J2219/00459 ,  B01J2219/00576 ,  B01J2219/00608 ,  B01J2219/00648 ,  B01J2219/00722 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2537/143 ,  C12Q2565/501 ,  C12Q2565/507 ,  C12Q2533/101 ,  C12Q2565/514 ,  C12Q2565/519

Abstract: Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including a method of preventing a significant reduction in duplexes detectable in a hybridization assay involving (i) selecting probe lengths for sets of oligonucleotide probes, wherein probes include different subsequences such that at least one subsequence is complementary to a subsequence in a cognate target; wherein probes for longer cognate targets are longer in length than probes for shorter cognate targets, (ii) selecting, for each set of probes, a density of oligonucleotides probes attached per unit area on a solid phase carrier which is below a limit at which the significant reduction in detectable duplexes is predicated to take place, (iii) producing the probes and affixing them to different solid phase carriers at the selected density, and (iv) annealing targets to the probes, wherein signal intensities of probes and targets of different lengths are about the same.

公开(公告)号：US20130123146A1

公开(公告)日：2013-05-16

申请号：US13664953

申请日：2012-10-31

Applicant: Bioarray Solutions, Ltd.

Inventor： Michael Seul

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00713 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0877 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0454 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/00 ,  C40B40/04 ,  G01N15/1468 ,  G01N27/447 ,  G01N33/54313 ,  G01N33/54386 ,  G01N33/6845 ,  G01N2015/149 ,  G01N2015/1497 ,  G02B3/0012 ,  G02B3/0056 ,  G02B3/0075 ,  H05H3/04 ,  Y10S435/808 ,  Y10S435/81 ,  Y10S435/814 ,  Y10S436/806 ,  Y10S436/808 ,  Y10S436/809 ,  Y10T436/25125 ,  C12Q2565/501 ,  C12Q2563/155 ,  C12Q2565/607

Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components.

Abstract translation: 一种用于在诸如氧化硅的绝缘电极和电解质溶液之间的界面处操作胶体颗粒和生物分子的方法和装置。 表面附近颗粒的光控电动组装取决于三个功能元件的组合：交流电场引起的平面聚集体的组装; 电解质/氧化硅/硅界面的图案化以在组装过程上施加空间控制; 并通过外部照明实现对装配过程的实时控制。 本发明提供一组基本操作，使得能够对几种类型的颗粒和生物分子的平面阵列的创建和放置以及阵列形状和尺寸的操纵进行交互式控制。 本发明使得能够以阵列格式进行诊断测定和生化分析的样品制备和处理以及这些操作的功能整合。 此外，本发明提供了用于产生具有所需性质的材料表面和用于制造表面安装光学部件的步骤。

公开(公告)号：US09637777B2

公开(公告)日：2017-05-02

申请号：US14257294

申请日：2014-04-21

Applicant: BioArray Solutions, Ltd.

Inventor： Michael Seul ,  Sukanta Banerjee ,  Jiacheng Yang ,  Tatiana Vener

IPC: C12Q1/68 ,  C12M1/34 ,  C12P19/34 ,  C07H21/04

CPC classification number: C12Q1/6832 ,  B01J2219/00459 ,  B01J2219/00576 ,  B01J2219/00608 ,  B01J2219/00648 ,  B01J2219/00722 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q2537/143 ,  C12Q2565/501 ,  C12Q2565/507 ,  C12Q2533/101 ,  C12Q2565/514 ,  C12Q2565/519

Abstract: Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including a method of preventing a significant reduction in duplexes detectable in a hybridization assay involving (i) selecting probe lengths for sets of oligonucleotide probes, wherein probes include different subsequences such that at least one subsequence is complementary to a subsequence in a cognate target; wherein probes for longer cognate targets are longer in length than probes for shorter cognate targets, (ii) selecting, for each set of probes, a density of oligonucleotides probes attached per unit area on a solid phase carrier which is below a limit at which the significant reduction in detectable duplexes is predicated to take place, (iii) producing the probes and affixing them to different solid phase carriers at the selected density, and (iv) annealing targets to the probes, wherein signal intensities of probes and targets of different lengths are about the same.

公开(公告)号：US09400259B2

公开(公告)日：2016-07-26

申请号：US14181028

申请日：2014-02-14

Applicant: Bioarray Solutions, Ltd

Inventor： Michael Seul

IPC: G01N33/543 ,  G01N27/447 ,  B01J19/00 ,  C12Q1/68 ,  C40B40/04 ,  G01N33/68 ,  G02B3/00 ,  H05H3/04 ,  B01L3/00 ,  C40B40/00 ,  G01N15/14

CPC classification number: B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00713 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0877 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0454 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/00 ,  C40B40/04 ,  G01N15/1468 ,  G01N27/447 ,  G01N33/54313 ,  G01N33/54386 ,  G01N33/6845 ,  G01N2015/149 ,  G01N2015/1497 ,  G02B3/0012 ,  G02B3/0056 ,  G02B3/0075 ,  H05H3/04 ,  Y10S435/808 ,  Y10S435/81 ,  Y10S435/814 ,  Y10S436/806 ,  Y10S436/808 ,  Y10S436/809 ,  Y10T436/25125 ,  C12Q2565/501 ,  C12Q2563/155 ,  C12Q2565/607

Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relics on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components.

Abstract translation: 一种用于在诸如氧化硅的绝缘电极和电解质溶液之间的界面处操作胶体颗粒和生物分子的方法和装置。 表面附近颗粒的光控电动组装依靠三个功能元件的组合：交流电场引起的平面聚集体组装; 电解质/氧化硅/硅界面的图案化以在组装过程上施加空间控制; 并通过外部照明实现对装配过程的实时控制。 本发明提供一组基本操作，使得能够对几种类型的颗粒和生物分子的平面阵列的创建和放置以及阵列形状和尺寸的操纵进行交互式控制。 本发明使得能够以阵列格式进行诊断测定和生化分析的样品制备和处理以及这些操作的功能整合。 此外，本发明提供了用于产生具有所需性质的材料表面和用于制造表面安装光学部件的步骤。

公开(公告)号：US20160152781A1

公开(公告)日：2016-06-02

申请号：US15018297

申请日：2016-02-08

Applicant: BioArray Solutions, Ltd.

Inventor： Sukanta BANERJEE ,  Cecila Georgescu ,  Michael Seul

IPC: C08J3/21 ,  C08J3/22

CPC classification number: C08J3/212 ,  C08J3/215 ,  C08J3/22 ,  C08J2325/08

Abstract: Solute-loaded polymer microparticles are obtained by immersing microparticles in a bath comprising a selected solute dissolved in a ternary solvent system. A first solvent of the ternary system is a strong solvent for both the solute and the polymer from which the microparticle was formed. A second solvent is a weak solvent or non-solvent for the solute and the polymer (tuning solvent). A third solvent is a weak solvent or non-solvent for the solute and polymer, but serves as a co-solvent with respect to the first and second solvents in that it is miscible with both the first and second solvents. The amount of solute incorporated into the microparticles is controlled by adjusting the ratio of solute with respect to the microparticle polymer, and by adjusting the composition of the ternary solvent system, principally the amount of tuning solvent. The method is particularly useful for providing libraries of combinatorially encoded microparticles containing distinguishable dye loadings, particularly distinguishable fluorescent dye loadings.

Abstract translation: 通过将微粒浸入包含溶解在三元溶剂系统中的选定溶质的浴中来获得溶质负载的聚合物微粒。 三元体系的第一溶剂是溶质和形成微粒的聚合物的强溶剂。 第二溶剂是用于溶质和聚合物（调谐溶剂）的弱溶剂或非溶剂。 第三溶剂是用于溶质和聚合物的弱溶剂或非溶剂，但是作为相对于第一和第二溶剂的共溶剂，因为其可与第一溶剂和第二溶剂混溶。 通过调节溶质相对于微粒聚合物的比例，调节三元溶剂体系的组成，主要是调谐溶剂的量来控制掺入到微粒中的溶质的量。 该方法对于提供包含可区分的染料负载，特别是可区分的荧光染料负载的组合编码的微粒的文库是特别有用的。

公开(公告)号：US09251583B2

公开(公告)日：2016-02-02

申请号：US14202357

申请日：2014-03-10

Applicant: Bioarray Solutions, Ltd.

Inventor： Michael Seul ,  Xiongwu Xia ,  Chiu Chau ,  Scott Determan

IPC: G06K9/00 ,  G06T7/00

CPC classification number: G06T7/0012 ,  G01N33/566 ,  G06K9/00 ,  G06K9/00147 ,  G06K2209/07 ,  G06T7/11 ,  G06T7/155 ,  G06T7/33 ,  G06T2207/10064 ,  G06T2207/20152 ,  G06T2207/30072

Abstract: Systems and methods are provided the autocentering, autofocusing, acquiring, decoding, aligning, analyzing and exchanging among various parties, images, where the images are of arrays of signals associated with ligand-receptor interactions, and more particularly, ligand-receptor interactions where a multitude of receptors are associated with microparticles or microbeads. The beads are encoded to indicate the identity of the receptor attached, and therefore, an assay image and a decoding image are aligned to effect the decoding. The images or data extracted from such images can be exchanged between de-centralized assay locations and a centralized location where the data are analyzed to indicate assay results. Access to data can be restricted to authorized parties in possession of certain coding information, so as to preserve confidentiality.

公开(公告)号：US20150126407A1

公开(公告)日：2015-05-07

申请号：US14486598

申请日：2014-09-15

Applicant: Bioarray Solutions, Ltd.

Inventor： Michael Seul ,  Chiu Wo Chau ,  Hui Huang ,  Sukanta Banerjee ,  Jiacheng Yang ,  Ye Hong

IPC: C12Q1/68 ,  G01N33/543

CPC classification number: C12Q1/6837 ,  B01J19/0046 ,  B01J2219/00274 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00648 ,  B01J2219/00662 ,  G01N33/54306 ,  C12Q2563/149

Abstract: This invention provides high unit density arrays of microparticles and methods of assembling such arrays. The microparticles in the arrays may be functionalized with chemical or biological entities specific to a given target analyte. The high unit density arrays of this invention are formed on chips which may be combined to form multichip arrays according to the methods described herein. The chips and/or multichip arrays of this invention are useful for chemical and biological assays.

Abstract translation: 本发明提供高单位密度的微粒阵列和组装这种阵列的方法。 阵列中的微粒可以用给定靶分析物特异性的化学或生物实体来官能化。 本发明的高单位密度阵列形成在芯片上，芯片可以根据本文所述的方法组合以形成多芯片阵列。 本发明的芯片和/或多芯片阵列可用于化学和生物测定。