公开(公告)号：US20170044572A1

公开(公告)日：2017-02-16

申请号：US15078437

申请日：2016-03-23

Applicant: Genomatica, Inc.

Inventor： Mark J. BURK ,  Anthony P. BURGARD ,  Jun SUN ,  Robin E. OSTERHOUT ,  Priti PHARKYA

IPC: C12P5/02 ,  C12N15/52

CPC classification number: C12P5/02 ,  C07C1/24 ,  C07C2521/04 ,  C07C2521/12 ,  C07C2521/16 ,  C07C2523/10 ,  C07C2523/28 ,  C07C2523/31 ,  C07C2523/745 ,  C07C2527/167 ,  C07C2527/173 ,  C12N15/52 ,  C12P5/026 ,  C12P7/04 ,  Y02E50/343 ,  C07C11/167

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

Abstract translation: 本发明提供具有丁二烯途径的非天然存在的微生物。 本发明另外提供了使用这些生物体来生产丁二烯的方法。

公开(公告)号：US20160326553A1

公开(公告)日：2016-11-10

申请号：US15108259

申请日：2014-12-23

Applicant: GENOMATICA, INC.

Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Stephen J. VAN DIEN ,  Priti PHARKYA ,  Tae Hoon YANG ,  Jungik CHOI

IPC: C12P7/18 ,  C12N9/02 ,  C12N15/52 ,  C12N9/52 ,  C12N9/88 ,  C12N9/16 ,  C12N9/12

CPC classification number: C12P7/18 ,  C07K14/245 ,  C12N1/20 ,  C12N9/0036 ,  C12N9/0057 ,  C12N9/1205 ,  C12N9/16 ,  C12N9/52 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/42 ,  C12Y106/01002 ,  C12Y110/03 ,  C12Y207/0104 ,  C12Y301/00 ,  C12Y301/02 ,  C12Y301/02002 ,  C12Y304/21092 ,  C12Y401/01031 ,  C12Y401/01032

Abstract: The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.

Abstract translation: 本发明涉及一种非天然存在的微生物生物体，其包含琥珀酰-CoA合成酶或转移酶的第一次衰减，以及琥珀酰-CoA转化酶或编码多步骤中产琥珀酸酶的基因的至少第二次衰减 途径具有琥珀酰-CoA向琥珀酸盐的净转化。

公开(公告)号：US20160319313A1

公开(公告)日：2016-11-03

申请号：US14954487

申请日：2015-11-30

Applicant: Genomatica, Inc.

Inventor： Jun SUN ,  Mark J. BURK ,  Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Wei NIU ,  John D. TRAWICK ,  Robert HASELBECK

IPC: C12P17/04 ,  C12N15/52 ,  C12P7/18

CPC classification number: C12P17/04 ,  C12N1/20 ,  C12N15/52 ,  C12P7/18

Abstract: The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.

Abstract translation: 本发明提供包含1,4-丁二醇（14-BDO）和γ-丁内酯（GBL）途径的非天然存在的微生物生物，其包含至少一种编码14-BDO和GBL途径酶的外源核酸，其以足够的量表达， 生产14-BDO和GBL。 本发明另外提供了使用这种微生物生物来生产14-BDO和GBL的方法。

公开(公告)号：US20150064750A1

公开(公告)日：2015-03-05

申请号：US14226631

申请日：2014-03-26

Applicant: Genomatica, Inc.

Inventor： Robin E. OSTERHOUT ,  Anthony P. Burgard ,  Priti Pharkya ,  Mark J. Burk

IPC: C12P7/62 ,  C12P5/02

CPC classification number: C12P7/62 ,  C12N15/52 ,  C12P5/005 ,  C12P5/026 ,  C12P7/16 ,  C12P7/40 ,  C12P9/00 ,  Y02E50/10

Abstract: The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.

Abstract translation: 本发明提供了具有甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯 ，2,4-戊二烯酸酯，3-丁烯-1醇或1,3-丁二烯途径。 本发明另外提供了使用这些生物体来生产甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯 ，2,4-戊二烯酸酯，3-丁烯-1醇或1,3-丁二烯。

公开(公告)号：US20220220511A1

公开(公告)日：2022-07-14

申请号：US17394184

申请日：2021-08-04

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Priti PHARKYA ,  Stefan ANDRAE

IPC: C12P5/02 ,  C12P7/04 ,  C12P7/18 ,  C12P7/16 ,  C12N15/52

Abstract: Provided herein are non-naturally occurring microbial organisms having a formaldehyde fixation pathway and a formate assimilation pathway, which can further include a methanol metabolic pathway, a methanol oxidation pathway, a hydrogenase and/or a carbon monoxide dehydrogenase. These microbial organisms can further include a butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol pathway. Additionally provided are methods of using such microbial organisms to produce butadiene, 1,3-butanediol, crotyl alcohol or 3-buten-2-ol.

公开(公告)号：US20190264242A1

公开(公告)日：2019-08-29

申请号：US16137264

申请日：2018-09-20

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Jun SUN ,  Priti PHARKYA

IPC: C12P7/42 ,  C12P7/18 ,  C12P7/16 ,  C12P7/52 ,  C12N9/02 ,  C12P7/04

Abstract: A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a building block. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.

公开(公告)号：US20170183694A1

公开(公告)日：2017-06-29

申请号：US15191421

申请日：2016-06-23

Applicant: Genomatica, Inc.

Inventor： Priti PHARKYA ,  Anthony P. BURGARD ,  Stephen J. VAN DIEN ,  Robin E. OSTERHOUT ,  Mark J. BURK ,  John D. TRAWICK ,  Michael P. KUCHINSKAS ,  Brian STEER ,  Stefan ANDRAE ,  Amit SHAH

IPC: C12P7/18 ,  C12P7/42 ,  C12N1/20 ,  C12P17/04 ,  C07C211/09 ,  C12P13/00 ,  C07C47/19 ,  C07C31/20 ,  C07D307/33 ,  C12N15/52 ,  C12N9/10

CPC classification number: C12P7/18 ,  C07C31/207 ,  C07C47/19 ,  C07C211/09 ,  C07D307/33 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/13 ,  C12N15/52 ,  C12P7/24 ,  C12P7/42 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P17/04 ,  C12P19/32 ,  C12Y208/03

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.

公开(公告)号：US20170073691A1

公开(公告)日：2017-03-16

申请号：US15133150

申请日：2016-04-19

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Stephen J. VAN DIEN ,  Cara Ann TRACEWELL ,  Priti PHARKYA ,  Stefan ANDRAE

IPC: C12N15/52 ,  C12N9/10 ,  C12N9/06 ,  C12P7/62 ,  C12N9/04

CPC classification number: C12N15/52 ,  C08F120/06 ,  C08G63/664 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N15/63 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  C12Y101/01244 ,  C12Y105/0102 ,  C12Y201/0109

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.

Abstract translation: 本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这种还原当量可用于增加由微生物生物体如3-羟基异丁酸酯或MAA产生的有机化合物的产物产率。 本文还提供了使用这种生物体产生3-羟基异丁酸酯或MAA的方法。

公开(公告)号：US20160355846A1

公开(公告)日：2016-12-08

申请号：US15148759

申请日：2016-05-06

Applicant: Genomatica, Inc.

Inventor： Stephen J. VAN DIEN ,  Anthony P. BURGARD ,  Robert HASELBECK ,  Catherine J. PUJOL-BAXLEY ,  Wei NIU ,  John D. TRAWICK ,  Harry YIM ,  Mark J. BURK ,  Robin E. OSTERHOUT ,  Jun SUN

IPC: C12P7/18 ,  C12N9/10 ,  C12N15/52 ,  C12N9/16 ,  C12N9/02 ,  C12N9/04 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

Abstract translation: 本发明提供了包含1,4-丁二醇（BDO）途径的非天然存在的微生物生物，其包含至少一种编码足够量的BDO途径酶的外源核酸以产生BDO并进一步优化用于BDO的表达。 本发明还提供了使用这种微生物生产BDO的方法。

公开(公告)号：US20150167028A1

公开(公告)日：2015-06-18

申请号：US14450150

申请日：2014-08-01

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Priti PHARKYA ,  Robin E. OSTERHOUT

IPC: C12P7/46 ,  C12N9/02 ,  C12P17/10 ,  C12N9/80 ,  C12N9/88 ,  C12P13/00 ,  C12N9/04 ,  C12N9/10

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.