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公开(公告)号：US11932900B2
公开(公告)日：2024-03-19
申请号：US17973327
申请日：2022-10-25
Applicant: ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED
Inventor： Wayne N. George , Alexandre Richez , M. Shane Bowen , Andrew A. Brown , Dajun Yuan , Audrey Rose Zak , Sean M. Ramirez , Raymond Campos
IPC: C08J7/04 , C09D133/26 , C09D183/06 , C12Q1/68 , C08G77/14
CPC classification number: C12Q1/68 , C09D133/26 , C09D183/06 , C08G77/14
Abstract: An example of an array includes a support, a cross-linked epoxy polyhedral oligomeric silsesquioxane (POSS) resin film on a surface of the support, and a patterned hydrophobic polymer layer on the cross-linked epoxy POSS resin film. The patterned hydrophobic polymer layer defines exposed discrete areas of the cross-linked epoxy POSS resin film, and a polymer coating is attached to the exposed discrete areas. Another example of an array includes a support, a modified epoxy POSS resin film on a surface of the support, and a patterned hydrophobic polymer layer on the modified epoxy POSS resin film. The modified epoxy POSS resin film includes a polymer growth initiation site, and the patterned hydrophobic polymer layer defines exposed discrete areas of the modified epoxy POSS resin film. A polymer brush is attached to the polymer growth initiation site in the exposed discrete areas.
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公开(公告)号：US20230272469A1
公开(公告)日：2023-08-31
申请号：US18195321
申请日：2023-05-09
Applicant: ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED , ILLUMINA SINGAPORE PTE. LTD.
Inventor： Sean M. Ramirez , Brian D. Mather , Edwin Li , Sojeong Moon , Innsu Daniel Kim , Alexandre Richez , Ludovic Vincent , Xavier von Hatten , Hai Quang Tran , Maxwell Zimmerley , Julia Morrison , Gianluca Andrea Artioli , Krystal Sly , Hayden Black , Lewis J. Kraft , Hong Xie , Wei Wei , Ryan Sanford
IPC: C12Q1/6874 , C12Q1/6806 , B01L3/00
CPC classification number: C12Q1/6874 , C12Q1/6806 , B01L3/502707 , B01L2300/165 , B01L2300/0819 , B01L2300/0887 , B01L2300/16 , B01L2300/0877
Abstract: An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.
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公开(公告)号：US20210208055A1
公开(公告)日：2021-07-08
申请号：US17191612
申请日：2021-03-03
Applicant: ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED
Inventor： James Tsay , Anmiv Prabhu , David Heiner , Edwin Li , Alexandre Richez , John M. Beierle , Kevan Samiee , Kristina Munoz , Leonid Malevanchik , Ludovic Vincent , Naiqian Zhan , Peyton Shieh , Robert Yang , Samantha Schmitt , Sang Park , Scott Bailey , Sean M. Ramirez , Sunmin Ahn , Valerie Uzzell , Wei Wei , Yuxiang Huang , Tyler Jamison Dill
IPC: G01N15/14 , C12Q1/6874 , B01L3/00 , C09D133/24 , C09D133/26 , B01J19/00
Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.
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公开(公告)号：US20180178416A1
公开(公告)日：2018-06-28
申请号：US15847150
申请日：2017-12-19
Applicant: Illumina, Inc. , Illumina Cambridge Limited
Inventor： Alexandre Richez , Andrew A. Brown , Julia Morrison , Wayne N. George , Timothy J. Merkel , Audrey Rose Zak
Abstract: An imprinting apparatus includes a silicon master having a plurality of nanofeatures defined therein. An anti-stick layer coats the silicon master, the anti-stick layer including a molecule having a cyclosiloxane with at least one silane functional group. A method includes forming a master template by: depositing a formulation on a silicon master including a plurality of nanofeatures defined therein, the formulation including a solvent and a molecule having a cyclosiloxane with at least one silane functional group; and curing the formulation, thereby forming an anti-stick layer on the silicon master, the anti-stick layer including the molecule. The method further includes depositing a silicon-based working stamp material on the anti-stick layer of the master template; curing the silicon-based working stamp material to form a working stamp including a negative replica of the plurality of nanofeatures; and releasing the working stamp from the master template.
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公开(公告)号：US20250161882A1
公开(公告)日：2025-05-22
申请号：US18947506
申请日：2024-11-14
Applicant: ILLUMINA, INC.
Inventor： Yuliia Vyborna , Istvan Kocsis , Alexandre Richez , Davide Garoldini
Abstract: An example of a fluidic device includes a support structure, which defines a well and an interstitial region surrounding the well. An electrode is operatively positioned at a bottom of the well. Membrane fragments are bound over the interstitial region, and a nanopore, a nanopore subunit, or a combination thereof is bound over the interstitial region. The fluidic device may be incorporated into a nanopore instrument.
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Patent Agency Ranking
公开(公告)号：US20250101415A1
公开(公告)日：2025-03-27
申请号：US18575265
申请日：2023-04-24
Applicant: Illumina, Inc.
Inventor： Miguel Angel Aleman Garcia , Alexandre Richez
IPC: C12N15/11 , B82Y5/00 , C12Q1/6869
Abstract: The present application discloses a novel DNA origami structure and a nanopore construct associated with the DNA origami structure. The DNA origami structure includes a first hydrophilic section at a first end of the DNA origami structure, a stopper section adjacent the first hydrophilic section, a second hydrophilic section at a second end of the DNA origami structure, a hydrophobic section between the stopper section and the second hydrophilic section, and an open cavity running through the DNA origami structure from the first end to the second end. The stopper section is configured to lay against the membrane when the DNA origami structure is inserted through the membrane.
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公开(公告)号：US20240392117A1
公开(公告)日：2024-11-28
申请号：US18653173
申请日：2024-05-02
Applicant: Illumina, Inc.
Inventor： Antonio Conde-Gonzalez , Davide Garoldini , Yuliia Vyborna , Charlotte Vacogne , Istvan Kocsis , Oliver Uttley , Miguel Angel Aleman Garcia , Alexandre Richez
Abstract: Methods of making barriers including nanopores and crosslinked amphiphilic molecules, and barriers made using the same, are provided herein. In some examples, a method of forming a barrier between first and second fluids includes forming at least one layer comprising a plurality of amphiphilic molecules, wherein the amphiphilic molecules comprise reactive moieties. The method may include using first crosslinking reactions of the reactive moieties to only partially crosslink amphiphilic molecules of the plurality to one another. The method may include, after using the first crosslinking reactions, inserting the nanopore into the at least one layer. The method may include, after inserting the nanopore, using second crosslinking reactions of the reactive moieties to further crosslink amphiphilic molecules of the plurality to one another.
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公开(公告)号：US11846580B2
公开(公告)日：2023-12-19
申请号：US17191612
申请日：2021-03-03
Applicant: ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED
Inventor： James Tsay , Anmiv Prabhu , David Heiner , Edwin Li , Alexandre Richez , John M. Beierle , Kevan Samiee , Kristina Munoz , Leonid Malevanchik , Ludovic Vincent , Naiqian Zhan , Peyton Shieh , Robert Yang , Samantha Schmitt , Sang Park , Scott Bailey , Sean M. Ramirez , Sunmin Ahn , Valerie Uzzell , Wei Wei , Yuxiang Huang , Tyler Jamison Dill
IPC: G01N15/14 , C12Q1/6874 , B01L3/00 , C09D133/24 , C09D133/26 , G01N15/10 , B01J19/00
CPC classification number: G01N15/1436 , B01J19/0046 , B01L3/502 , B01L3/502707 , C09D133/24 , C09D133/26 , C12Q1/6874 , G01N15/1484 , B01J2219/00286 , B01J2219/00317 , B01J2219/00319 , B01J2219/00418 , B01J2219/00585 , B01J2219/00596 , B01J2219/00612 , B01J2219/00637 , B01J2219/00722 , B01L2200/12 , B01L2300/087 , B01L2300/0829 , B01L2300/0848 , B01L2300/0887 , B01L2300/16 , G01N2015/1006
Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.
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公开(公告)号：US10808282B2
公开(公告)日：2020-10-20
申请号：US15739688
申请日：2016-07-05
Applicant: ILLUMINA, INC.
Inventor： Andrew A. Brown , Wayne N. George , Alexandre Richez , M. Shane Bowen
IPC: C12Q1/68 , C12Q1/6874 , G03F7/00 , G03F7/16 , G01N21/64
Abstract: Substrates comprising dual functional polymer layered surfaces and the preparation thereof by using UV nano-imprinting processes are disclosed. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.
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公开(公告)号：US12110547B2
公开(公告)日：2024-10-08
申请号：US17033237
申请日：2020-09-25
Applicant: Illumina, Inc.
Inventor： Andrew A. Brown , Wayne N. George , Alexandre Richez , M. Shane Bowen
CPC classification number: C12Q1/6874 , G03F7/0002 , G03F7/161 , B01L3/5085 , B01L2200/12 , B01L2300/0819 , B01L2300/0829 , B01L2300/161 , G01N21/645
Abstract: Substrates comprising dual functional polymer layered surfaces and the preparation thereof by using UV nano-imprinting processes are disclosed. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.
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