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公开(公告)号：US20220411859A1
公开(公告)日：2022-12-29
申请号：US16609727
申请日：2018-08-01
Applicant: Illumina, Inc.
Inventor： Yir-Shyuan Wu , Xi-Jun Chen , Filiz Gorpe-Yasar , Falko Noé , Charles Lin , Tarun Kumar Khurana , Foad Mashayekhi , Pallavi Daggumati , Frank J Steemers , Kevin Gunderson , Jeffrey S Fisher
IPC: C12Q1/6869 , C12N15/10 , C12Q1/6806
Abstract: Systems, methods, and compositions provided herein relate to preparation of beads encapsulating genetic material. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material.
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公开(公告)号：US11318462B2
公开(公告)日：2022-05-03
申请号：US16750930
申请日：2020-01-23
Applicant: ILLUMINA, INC.
Inventor： Tarun Kumar Khurana , Arnaud Rival , Lewis J. Kraft , Steven Barnard , M. Shane Bowen , Xi-Jun Chen , Yir-Shyuan Wu , Jeffrey S. Fisher , Dajun Yuan
Abstract: An example of a flow cell includes a substrate, which includes nano-depressions defined in a surface of the substrate, and interstitial regions separating the nano-depressions. A hydrophobic material layer has a surface that is at least substantially co-planar with the interstitial regions and is positioned to define a hydrophobic barrier around respective sub-sets of the nano-depressions.
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公开(公告)号：US20210230585A1
公开(公告)日：2021-07-29
申请号：US17158926
申请日：2021-01-26
Applicant: ILLUMINA, INC.
Inventor： Tyler Chen , Pallavi Daggumati , Tarun Kumar Khurana , Yir-Shyuan Wu
Abstract: An example of a kit includes a library preparation fluid, a sample fluid, and an enrichment fluid. The library preparation fluid includes library preparation beads, where each library preparation bead includes a first solid support, and a transposome attached to the first solid support. The fluid includes a genomic deoxyribonucleic acid sequence. The enrichment fluid includes target capture beads, where each target capture bead includes a second solid support, and capture probes attached to the second solid support. Each of the capture probes includes a single stranded deoxyribonucleic acid sequence that is complementary to a targeted region of the genomic deoxyribonucleic acid in the sample fluid.
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14.发明申请公开(公告)号：US20200216895A1
公开(公告)日：2020-07-09
申请号：US16626115
申请日：2018-07-31
Applicant: ILLUMINA, INC.
Inventor： Tarun Kumar Khurana , Yir-Shyuan Wu , Xi-Jun Chen , Filiz Gorpe-Yasar , Yan-You Lin , Victoria Popic , Erich B. Jaeger , Mostafa Ronaghi
IPC: C12Q1/6874 , C12Q1/6813
Abstract: Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.
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公开(公告)号：US20200086322A1
公开(公告)日：2020-03-19
申请号：US16691405
申请日：2019-11-21
Applicant: Illumina, Inc.
Inventor： Hamed Amini , Arash Jamshidi , Tarun Kumar Khurana , Foad Mashayekhi , Yir-Shyuan Wu
Abstract: Described are microfluidic devices and methods for providing a predetermined number of microspheres or beads, together with a cell, within a fluid droplet being processed. The system may provide each droplet with a single bead and a single cell, and the bead may contain DNA or other reagents for later identifying the specific cell associated with that bead.
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Patent Agency Ranking
公开(公告)号：US20170128940A1
公开(公告)日：2017-05-11
申请号：US15347709
申请日：2016-11-09
Applicant: Illumina, Inc.
Inventor： Hamed Amini , Arash Jamshidi , Tarun Kumar Khurana , Foad Mashayekhi , Yir-Shyuan Wu
IPC: B01L3/00
CPC classification number: B01L3/502761 , B01F3/0807 , B01F13/0062 , B01J2219/00468 , B01J2219/005 , B01J2219/00722 , B01L3/502776 , B01L3/502784 , B01L2200/0636 , B01L2200/0647 , B01L2200/0652 , B01L2300/0816 , B01L2300/0858 , B01L2300/0867 , B01L2300/0883
Abstract: Described are microfluidic devices and methods for providing a predetermined number of microspheres or beads, together with a cell, within a fluid droplet being processed. The system may provide each droplet with a single bead and a single cell, and the bead may contain DNA or other reagents for later identifying the specific cell associated with that bead.
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公开(公告)号：US20250091042A1
公开(公告)日：2025-03-20
申请号：US18965657
申请日：2024-12-02
Applicant: ILLUMINA, INC.
Inventor： Lewis J. Kraft , Tarun Kumar Khurana , Yir-Shyuan Wu , Xi-Jun Chen , Arnaud Rival , Justin Fullerton , M. Shane Bowen , Hui Han , Jeffrey S. Fisher , Yasaman Farshchi , Mathieu Lessard-Viger
Abstract: An example of a flow cell includes a substrate, a plurality of chambers defined on or in the substrate, and a plurality of depressions defined in the substrate and within a perimeter of each of the plurality of chambers. The depressions are separated by interstitial regions. Primers are attached within each of the plurality of depressions, and a capture site is located within each of the plurality of chambers.
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公开(公告)号：US11819843B2
公开(公告)日：2023-11-21
申请号：US17729814
申请日：2022-04-26
Applicant: ILLUMINA, INC.
Inventor： Tarun Kumar Khurana , Arnaud Rival , Lewis J. Kraft , Steven Barnard , M. Shane Bowen , Xi-Jun Chen , Yir-Shyuan Wu , Jeffrey S. Fisher , Dajun Yuan
CPC classification number: B01L3/5027 , B01L3/5085 , C12N15/1065 , B01L3/5088 , B01L2300/0896 , B01L2300/161 , B01L2300/165 , C08K5/5406 , C08L37/00
Abstract: An example of a flow cell includes a substrate, which includes nano-depressions defined in a surface of the substrate, and interstitial regions separating the nano-depressions. A hydrophobic material layer has a surface that is at least substantially co-planar with the interstitial regions and is positioned to define a hydrophobic barrier around respective sub-sets of the nano-depressions.
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公开(公告)号：US20230285963A1
公开(公告)日：2023-09-14
申请号：US18201054
申请日：2023-05-23
Applicant: ILLUMINA, INC.
Inventor： Bradley Drews , Reto Schoch , Tarun Kumar Khurana , Minsoung Rhee
IPC: B01L3/00
CPC classification number: B01L3/502715 , B01L3/50273 , B01L2200/16 , B01L2300/0867
Abstract: An apparatus includes one or more valves adapted to be coupled to corresponding reagent reservoirs and a flow cell interface adapted to be coupled to a flow cell. The apparatus includes a sample cartridge interface having one or more ports and adapted to be coupled to a sample cartridge carrying a sample of interest. The sample cartridge interface positioned downstream of the flow cell interface. The apparatus includes a pump adapted to load a channel of the flow cell with the sample of interest via the flow cell interface associated with an outlet of the flow cell and a corresponding port of the sample cartridge interface.
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20.发明公开公开(公告)号：US20230220462A1
公开(公告)日：2023-07-13
申请号：US18180622
申请日：2023-03-08
Applicant: ILLUMINA, INC.
Inventor： Tarun Kumar Khurana , Yir-Shyuan Wu , Xi-Jun Chen , Filiz Gorpe-Yasar , Yan-You Lin , Victoria Popic , Erich B. Jaeger , Mostafa Ronaghi
IPC: C12Q1/6874 , C12Q1/6813
CPC classification number: C12Q1/6874 , C12Q1/6813 , C12Q2600/156 , C12Q2565/514
Abstract: Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.
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