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公开(公告)号：US09194861B2
公开(公告)日：2015-11-24
申请号：US12862542
申请日：2010-08-24
CPC分类号： B01J19/0046 , B01F13/0071 , B01F13/0079 , B01J19/0093 , B01J2219/00286 , B01J2219/00418 , B01J2219/00495 , B01J2219/0059 , B01J2219/00599 , B01J2219/00722 , B01J2219/00873 , B01J2219/00894 , B01J2219/00903 , B01J2219/00957 , B01L3/502784 , B01L7/525 , B01L2200/0673 , B01L2200/16 , B01L2300/0867 , B01L2300/0883 , B01L2300/18 , B01L2400/0487 , B01L2400/0677 , C12Q1/6844 , C40B50/08 , C40B60/14 , G01N1/30 , G01N1/44 , G01N21/75 , G01N33/50 , G01N35/08 , C12Q2547/107
摘要： System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.
摘要翻译： 系统，包括方法，装置，组合物和试剂盒，用于通过多个乳液的聚结来混合小体积的流体。
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公开(公告)号：US20110212516A1
公开(公告)日：2011-09-01
申请号：US12890550
申请日：2010-09-24
IPC分类号： C12M1/00
CPC分类号： B01F3/0807 , B01F13/0062 , B01L3/0241 , B01L3/502784 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2300/0816 , B01L2300/0819 , B01L2300/087 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/0622
摘要： Thermocycling system, including methods and apparatus, for performing a flow-based reaction on a sample in fluid. The system may include a plurality of segments defining at least two temperature regions, and also may include a plurality of heating elements configured to maintain each temperature region at a different desired temperature. At least one of the heating elements may be a thermoelectric cooler operatively disposed to transfer heat to and/or from a temperature region The system further may include a fluid channel extending along a helical path that passes through the temperature regions multiple times such that fluid flowing in the channel is heated and cooled cyclically.
摘要翻译： 热循环系统，包括用于对流体中的样品进行基于流的反应的方法和装置。 该系统可以包括限定至少两个温度区域的多个段，并且还可以包括被配置为将每个温度区域保持在不同的期望温度的多个加热元件。 加热元件中的至少一个可以是可操作地设置成将热传递到和/或从温度区域传递的热电冷却器。该系统还可以包括沿着螺旋路径延伸的流体通道，该螺旋路径多次通过温度区域，使得流体流动 在通道中循环加热和冷却。
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公开(公告)号：US20110053798A1
公开(公告)日：2011-03-03
申请号：US12862542
申请日：2010-08-24
CPC分类号： B01J19/0046 , B01F13/0071 , B01F13/0079 , B01J19/0093 , B01J2219/00286 , B01J2219/00418 , B01J2219/00495 , B01J2219/0059 , B01J2219/00599 , B01J2219/00722 , B01J2219/00873 , B01J2219/00894 , B01J2219/00903 , B01J2219/00957 , B01L3/502784 , B01L7/525 , B01L2200/0673 , B01L2200/16 , B01L2300/0867 , B01L2300/0883 , B01L2300/18 , B01L2400/0487 , B01L2400/0677 , C12Q1/6844 , C40B50/08 , C40B60/14 , G01N1/30 , G01N1/44 , G01N21/75 , G01N33/50 , G01N35/08 , C12Q2547/107
摘要： System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.
摘要翻译： 系统，包括方法，装置，组合物和试剂盒，用于通过多个乳液的聚结来混合小体积的流体。
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公开(公告)号：US09126160B2
公开(公告)日：2015-09-08
申请号：US12963523
申请日：2010-12-08
CPC分类号： B01L3/502784 , B01F3/0807 , B01F13/0062 , B01F15/00922 , B01F2003/0834 , B01F2003/0842 , B01F2215/0037 , B01L3/0241 , B01L3/502715 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/12 , B01L2300/041 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0858 , B01L2300/0867 , B01L2300/1822 , B01L2400/0478 , B01L2400/0487 , B01L2400/049 , B01L2400/0622 , B29C45/0053 , B29C45/006 , B29C2045/0079 , B29L2031/752 , C12Q1/686 , G01N21/3563 , G01N21/49 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G06F19/00 , Y02A90/26
摘要： A system, including method and apparatus, for forming an array of emulsions. The system may include a plate providing an array of emulsion production units each configured to produce a separate emulsion and each including a set of wells interconnected by channels that intersect to form a site of droplet generation. Each set of wells, in turn, may include (1) at least one first input well to receive a continuous phase, (2) a second input well to receive a dispersed phase, and (3) an output well configured to receive from the site of droplet generation an emulsion of droplets of the dispersed phase disposed in the continuous phase.
摘要翻译： 一种用于形成乳剂阵列的系统，包括方法和装置。 该系统可以包括提供乳液生产单元阵列的板，每个乳液生产单元被配置为产生单独的乳液，并且每个包括通过相交以形成液滴产生位点的通道相互连接的一组孔。 每组井又可包括（1）至少一个用于接收连续相的第一输入井，（2）第二输入井以接收分散相，以及（3）输出孔，其构造成从 产生液滴的位置是分散相的液滴的乳液，其设置在连续相中。
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公开(公告)号：US20130045875A1
公开(公告)日：2013-02-21
申请号：US13562198
申请日：2012-07-30
IPC分类号： C40B30/00
CPC分类号： C40B20/04 , C12Q1/6806 , C12Q2545/114 , C12Q2563/159
摘要： Methods of characterizing a nucleic acid library by digital assay.
摘要翻译： 通过数字测定表征核酸文库的方法。
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16.发明授权Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture 有权在复杂混合物中排序，扩增，检测和鉴定核酸子序列公开(公告)号：US08338166B2
公开(公告)日：2012-12-25
申请号：US11650363
申请日：2007-01-04
CPC分类号： C12Q1/04 , B01L3/502753 , B01L3/502761 , B01L3/502784 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2300/1822 , B01L2300/1827 , B01L2300/1861 , B01L2400/0421 , B01L2400/0487 , C12Q1/6888 , G01N33/5432 , G01N33/569 , G01N33/587
摘要： A system for identifying all of the known and unknown pathogenic or non-pathogenic organisms in a sample. A droplet generator creates droplets from the sample. The droplets constitute sub-nanoliter volume reactors containing the organism sized particles. A lysis device performs lysis of the organisms to release the nucleic acids. An amplifier amplifies the nucleic acids. A fractionater releases the nucleic acids from the droplets. A parallel analyzer identifies all of the known and unknown pathogenic or non-pathogenic organisms in the sample.
摘要翻译： 用于鉴定样品中所有已知和未知的病原体或非致病生物体的系统。 液滴发生器从样品中产生液滴。 液滴构成含有生物体尺寸的颗粒的亚纳升体积反应器。 裂解装置进行生物体的裂解以释放核酸。 放大器放大核酸。 分馏器从液滴中释放出核酸。 并行分析仪识别样品中所有已知和未知的致病或非致病生物。
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公开(公告)号：US20110217712A1
公开(公告)日：2011-09-08
申请号：US12976827
申请日：2010-12-22
CPC分类号： C12Q1/6848 , C12Q1/6816 , C12Q1/6846 , C12Q1/686 , C12Q2527/125 , C12Q2563/159
摘要： System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.
摘要翻译： 用于制备和使用稳定化乳液的系统，包括方法，装置，组合物和试剂盒。 提供了一种产生稳定的乳液的方法。 在该方法中，可以提供水相。 水相可以包括有效浓度的一种或多种皮肤形成蛋白质。 可以形成乳液。 乳液可以包括设置在连续相中的分散相的液滴，水相是连续相或分散相。 可以加热乳液以在每个液滴和连续相之间产生界面皮肤，以将液滴转化成胶囊。
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公开(公告)号：US20100055677A1
公开(公告)日：2010-03-04
申请号：US11818623
申请日：2007-06-15
CPC分类号： C12Q1/04 , B01L3/502753 , B01L3/502761 , B01L3/502784 , B01L7/52 , B01L7/525 , B01L2200/0673 , B01L2200/10 , B01L2200/12 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0887 , B01L2300/1822 , B01L2300/1827 , B01L2300/1861 , B01L2400/0421 , B01L2400/0487 , C12Q1/6888 , G01N33/5432 , G01N33/569 , G01N33/587
摘要： A method of rapid, genome and proteome based identification of unknown pathogenic or non-pathogenic organisms in a complex sample. The entire sample is analyzed by creating millions of emulsion encapsulated microdroplets, each containing a single pathogenic or non-pathogenic organism sized particle and appropriate reagents for amplification. Following amplification, the amplified product is analyzed.
摘要翻译： 一种基于快速，基因组和蛋白质组学的鉴定复杂样本中未知致病或非致病生物的方法。 通过创建数百万个乳剂包封的微滴来分析整个样品，每个微滴含有单个致病或非致病生物体大小的颗粒和适当的扩增试剂。 扩增后，分析扩增产物。
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公开(公告)号：US09598725B2
公开(公告)日：2017-03-21
申请号：US12976827
申请日：2010-12-22
CPC分类号： C12Q1/6848 , C12Q1/6816 , C12Q1/6846 , C12Q1/686 , C12Q2527/125 , C12Q2563/159
摘要： System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.
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公开(公告)号：US20130189700A1
公开(公告)日：2013-07-25
申请号：US13558260
申请日：2012-07-25
IPC分类号： G01N1/34
CPC分类号： G01N1/34 , C12Q1/6806 , C12Q2563/159
摘要： Methods of processing an emulsion of aqueous droplets containing nucleic acid. The methods may include breakage of the emulsion with a destabilizing fluid including a halogen-substituted hydrocarbon.
摘要翻译： 处理含有核酸的水滴乳液的方法。 所述方法可包括用包含卤素取代的烃的不稳定流体破乳。
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