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    METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS
    13.
    发明申请
    METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS 有权

    公开(公告)号：US20210047686A1

    公开(公告)日：2021-02-18

    申请号：US16882414

    申请日：2020-05-22

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

    IPC: C12Q1/6874 ,  G01N27/414 ,  B01L3/00 ,  C12Q1/6869

    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

    Sequencing device
    14.
    发明授权
    Sequencing device 有权

    公开(公告)号：US10894982B2

    公开(公告)日：2021-01-19

    申请号：US16289508

    申请日：2019-02-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz ,  Todd Roswech ,  Jon A. Hoshizaki ,  Albert L. Carrillo ,  James A. Ball

    IPC: C12Q1/6869 ,  C12Q1/6806 ,  B01L3/00 ,  G01N27/414 ,  G01N35/10 ,  B01F5/06 ,  B01F11/00 ,  B01F1/00

    Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

    Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
    16.
    发明授权
    Phase-protecting reagent flow orderings for use in sequencing-by-synthesis 有权

    公开(公告)号：US10597711B2

    公开(公告)日：2020-03-24

    申请号：US13859360

    申请日：2013-04-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell ,  Jonathan Schultz

    IPC: C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6874

    Abstract: A method for nucleic acid sequencing includes: disposing a plurality of template polynucleotide strands, sequencing primers, and polymerases in a plurality of defined spaces of a sensor array; exposing template polynucleotide strands to a series of flows of nucleotide species, the series comprising a sequence of random flows; and obtaining, for each of the series of flows of nucleotide species, a signal indicative of how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands.

    Fluidics system for sequential delivery of reagents
    17.
    发明授权
    Fluidics system for sequential delivery of reagents 有权

    公开(公告)号：US10478816B2

    公开(公告)日：2019-11-19

    申请号：US15348907

    申请日：2016-11-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz ,  David Marran

    IPC: C12M1/00 ,  C12M3/00 ,  C12M1/34 ,  B01L3/00 ,  C12Q1/6869 ,  G01N27/447 ,  G01N35/10

    Abstract: The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion. The invention is particularly advantageous in apparatus for performing sensitive multistep reactions, such as pH-based DNA sequencing reactions.

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