公开(公告)号：US11417037B2

公开(公告)日：2022-08-16

申请号：US16937634

申请日：2020-07-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Harrison Leong ,  Nivedita Sumi Majumdar ,  Jeffrey A. Marks ,  Theodore E. Straub ,  Ryan J. Talbot ,  Kevin S. Bodner ,  David Hoard

IPC: G06T11/20 ,  G16B45/00

Abstract: A method for generating a data visualization is provided. The method includes displaying a representation of a portion of detected data from a substrate to a user. The method further includes generating a data quality value for the portion of detected data and displaying, along with the representation of the portion of detected data, an indication of data quality value for the portion of detected data. The method further includes selecting, by the user, a quality value threshold, and displaying an adjusted indication of data quality value for the portion of detected data meeting the quality value threshold.

公开(公告)号：US10323274B2

公开(公告)日：2019-06-18

申请号：US14348504

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jacob K. Freudenthal ,  Kevin Maher ,  Ming Song Chen ,  Theodore E. Straub ,  Kuan Moon Boo ,  Soo Yong Lau

IPC: C12Q1/686 ,  B01L3/00 ,  B01L7/00

Abstract: A biological analysis system is provided. The system comprises an interchangeable assembly configured to accommodate any one of a plurality of sample holders, each respective sample holder configured to receive a plurality of samples. The system also includes a control system configured to cycle the plurality of samples through a series of temperatures. The system further includes an optical system configured to detect fluorescent signals emitted from the plurality of samples. The optical system, in particular, can comprise a single field lens, an excitation source, an optical sensor, and a plurality of filter components. The excitation source can be one or more light emitting diodes. The field lens can be a bi-convex lens.

公开(公告)号：US20150080247A1

公开(公告)日：2015-03-19

申请号：US14385730

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael C. Pallas ,  James C. Nurse ,  Gary Lim ,  Theodore E. Straub ,  Eliodor Ghenciu ,  Evan W. Foster ,  Jorge Fonseca ,  Kevin Maher

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: B01L3/50857 ,  B01L3/50 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  C12Q1/6806 ,  C12Q1/686 ,  G03F7/2002

Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

Abstract translation: 提供了用于进行生物反应的系统。 该系统包括包括基底和多个反应位点的芯片。 多个反应位点各自被配置为包括至多一个纳升级的液体样品。 此外，该系统包括被配置为启动液体样品内的生物反应的控制系统。 该系统还包括被配置为检测芯片上的生物反应的检测系统。 根据各种实施方案，芯片包括至少20000个反应位点。 在其它实施方案中，芯片包括至少30000个反应位点。

公开(公告)号：US20150072897A1

公开(公告)日：2015-03-12

申请号：US14385735

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael C. Pallas ,  James C. Nurse ,  Gary Lim ,  Theodore E. Straub ,  Evan W. Foster

IPC: G01N35/10 ,  B01J19/00

CPC classification number: G01N35/1002 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/0038 ,  B01J2219/00599 ,  B01J2219/00619 ,  B01J2219/00637 ,  B01L3/5088 ,  B01L2200/0642 ,  B01L2200/12 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0406 ,  C40B60/14

Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85+/−15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.

Abstract translation: 提供了一种用于将液体样品加载到基底内的多个反应位点的样品装载器。 样品装载器包括第一叶片和联接到第一叶片的第二叶片。 样品装载器还包括在第一叶片和第二叶片之间的流动路径，其构造成将液体样品分配到包括多个反应位点的基底。 此外，在各种实施例中，液体样品与第一和第二叶片具有85 +/- 15度的前进接触角。 此外，从流动路径分配到多个反应位点的液体样品的装载可以基于毛细作用。

公开(公告)号：US20150051118A1

公开(公告)日：2015-02-19

申请号：US14385740

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eliodor Ghenciu ,  Theodore E. Straub ,  Evan W. Foster ,  Michael C. Pallas

IPC: B01L3/00

Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.

Abstract translation: 提供了用于生物反应的装置。 该装置包括衬底和衬底内的多个反应位点。 衬底的表面被配置为具有第一亲水性，并且多个反应位点的每个表面被配置为具有第二亲水性，以加载具有样品体积的大量反应位点。 每个加载的反应位点的样品体积基本上限于其各自的反应位点。 样品体积被配置为在反应位点内进行生物反应。

公开(公告)号：US20140213487A1

公开(公告)日：2014-07-31

申请号：US14348504

申请日：2012-09-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jacob K. Freudenthal ,  Geoffrey Dahlhoff ,  Kevin Maher ,  Ming Song Chen ,  Gary Lim ,  Ronald Danehy ,  David Fortescue ,  Ming Shen ,  Woon Liang Soh ,  Francis T. Cheng ,  Ruoyun Wu ,  Thiam Chye Lee ,  Gary A. Chappell ,  Chee Kiong Lim ,  Jeff Dere ,  Theodore E. Straub ,  Jorge Fonseca ,  Kuan Moon Boo ,  Soo Yong Lau

IPC: B01L7/00 ,  C12Q1/68

CPC classification number: C12Q1/686 ,  B01L3/50855 ,  B01L3/50857 ,  B01L7/52 ,  B01L2200/023 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/143 ,  B01L2300/021 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1827 ,  B01L2300/1844

Abstract: A biological analysis system is provided. The system comprises an interchangeable assembly configured to accommodate any one of a plurality of sample holders, each respective sample holder configured to receive a plurality of samples. The system also includes a control system configured to cycle the plurality of samples through a series of temperatures. The system further includes an optical system configured to detect fluorescent signals emitted from the plurality of samples. The optical system, in particular, can comprise a single field lens, an excitation source, an optical sensor, and a plurality of filter components. The excitation source can be one or more light emitting diodes. The field lens can be a bi-convex lens.

Abstract translation: 提供生物分析系统。 该系统包括被配置为容纳多个样品保持器中的任何一个的可互换组件，每个样品保持器被配置为接收多个样品。 该系统还包括被配置为使多个样品循环通过一系列温度的控制系统。 该系统还包括被配置为检测从多个样本发射的荧光信号的光学系统。 特别地，光学系统可以包括单个场透镜，激发源，光学传感器和多个滤光器组件。 激发源可以是一个或多个发光二极管。 场透镜可以是双凸透镜。