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公开(公告)号:US06681616B2
公开(公告)日:2004-01-27
申请号:US10008604
申请日:2001-11-09
申请人: Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill
发明人: Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill
IPC分类号: G01N1106
CPC分类号: G05D7/0694 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L9/527 , B01L2200/027 , B01L2300/0663 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , G01N11/08 , G01N27/44791 , G01N2035/1044 , Y10T137/8158
摘要: Microfluidic devices, systems, and methods measure viscosity, flow times, and/or other flow characteristics within the channels, and the measured flow characteristics can be used to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems, electrokinetic systems and/or other fluid transport mechanisms can generate the flow, controllably mix fluids, and the like.
摘要翻译: 微流体装置,系统和方法测量通道内的粘度,流动时间和/或其他流动特性,并且可以使用测量的流动特性来产生期望的流量。 多储层压力调节器和压力控制器系统,电动系统和/或其它流体输送机构可以产生流动,可控地混合流体等。
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公开(公告)号:US07566538B2
公开(公告)日:2009-07-28
申请号:US12017859
申请日:2008-01-22
申请人: J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
发明人: J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
CPC分类号: C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要: Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.
摘要翻译: 核苷酸和核苷酸类似物通过合成方法的并入/测序用于各种测序。 包含3'-封闭基团的核苷酸类似物用于提供可逆的链终止,用于通过合成进行测序。 典型的封闭基团包括磷酸基和氨基甲酸酯基。 使用荧光核苷酸进行测序,通过掺入荧光标记的核苷酸进行检测,任选地随后进行光漂白,并使用嵌入染料检测通过插入检测合成测序中的未标记核苷酸的添加。 微流控器件,包括粒子阵列,用于测序方法。
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公开(公告)号:US07344865B2
公开(公告)日:2008-03-18
申请号:US11460550
申请日:2006-08-11
申请人: J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
发明人: J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
CPC分类号: C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要: Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.
摘要翻译: 核苷酸和核苷酸类似物通过合成方法的并入/测序用于各种测序。 包含3'-封闭基团的核苷酸类似物用于提供可逆的链终止,用于通过合成进行测序。 典型的封闭基团包括磷酸基和氨基甲酸酯基。 使用荧光核苷酸进行测序,通过掺入荧光标记的核苷酸进行检测,任选地随后进行光漂白,并使用嵌入染料检测通过插入检测合成测序中的未标记核苷酸的添加。 微流控器件,包括粒子阵列,用于测序方法。
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公开(公告)号:US06613513B1
公开(公告)日:2003-09-02
申请号:US09510205
申请日:2000-02-22
申请人: J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
发明人: J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
IPC分类号: C12Q168
CPC分类号: C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要: Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.
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公开(公告)号:US06447661B1
公开(公告)日:2002-09-10
申请号:US09416288
申请日:1999-10-12
申请人: Andrea W. Chow , Robert S. Dubrow , J. Wallace Parce , Steven A. Sundberg , Jeffrey A. Wolk , Ring-Ling Chien , Steven James Gallagher , Michael R. Knapp , Anne R. Kopf-Sill , Tammy Burd Mehta
发明人: Andrea W. Chow , Robert S. Dubrow , J. Wallace Parce , Steven A. Sundberg , Jeffrey A. Wolk , Ring-Ling Chien , Steven James Gallagher , Michael R. Knapp , Anne R. Kopf-Sill , Tammy Burd Mehta
IPC分类号: G01N2726
CPC分类号: B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L2200/027 , B01L2300/0816 , B01L2300/0838 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , G01N2035/00237 , G01N2035/1062 , Y10S366/02 , Y10T436/2575
摘要: Methods, apparatus and systems are provided for introducing large numbers of different materials into a microfluidic analytical device rapidly, efficiently and reproducibly. In particular, improved integrated pipettor chip configurations, e.g. sippers or electropipettors, are described which are capable of sampling extremely small amounts of material for which analysis is desired, transporting material into a microfluidic analytical channel network, and performing the desired analysis on the material.
摘要翻译: 提供了方法,装置和系统,用于将大量不同的材料快速,有效和可再现地引入微流体分析装置。 特别地,改进的集成移液器芯片配置,例如 描述了能够对需要分析的极少量材料进行取样,将材料输送到微流体分析通道网络中并对材料进行所需分析的能力。
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公开(公告)号:US07040144B2
公开(公告)日:2006-05-09
申请号:US10207735
申请日:2002-07-26
申请人: Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill , Josh Molho , Anubhav Tripathi , Matthew B. Kerby
发明人: Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill , Josh Molho , Anubhav Tripathi , Matthew B. Kerby
CPC分类号: G01N27/44791 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L9/527 , B01L2200/027 , B01L2300/0663 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , G01N11/08 , G01N2035/1044 , G05D7/0694
摘要: Microfluidic devices, systems, and methods measure viscosity, flow times, and/or pressures, other flow characteristics within the channels, and the measured flow characteristics can be used to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems, electrokinetic systems and/or other fluid transport mechanisms can generate the flow, controllably mix fluids, and the like.
摘要翻译: 微流体装置,系统和方法测量通道内的粘度,流动时间和/或压力,其它流动特性,并且所测量的流动特性可用于产生所需的流量。 多储层压力调节器和压力控制器系统,电动系统和/或其它流体输送机构可以产生流动,可控地混合流体等。
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公开(公告)号:US09101928B2
公开(公告)日:2015-08-11
申请号:US13015242
申请日:2011-01-27
申请人: Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
发明人: Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
IPC分类号: G01N35/00 , B01L3/00 , C07H21/02 , C07H21/04 , C12Q1/68 , B01F13/00 , C40B60/14 , G01N27/447
CPC分类号: C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要: An array of transportable particle sets is used in a microfluidic device for performing chemical reactions in the microfluidic device. The microfluidic device comprises a main channel and intersecting side channels, the main channel and side channels forming a plurality of intersections. The array of particle sets is disposed in the main channel, and the side channels are coupled to reagents. As the particle sets are transported through the intersections of the main channel and the side channels, reagents are flowed through the side channels into contact with each array member (or selected array members), thereby providing a plurality of chemical reactions in the microfluidic system.
摘要翻译: 在微流体装置中使用可移动颗粒组的阵列,用于在微流体装置中执行化学反应。 微流体装置包括主通道和相交的侧通道,主通道和侧通道形成多个交叉点。 颗粒组的阵列设置在主通道中,并且侧通道与试剂偶联。 当颗粒组通过主通道和侧通道的交叉点传输时,试剂通过侧通道流动与每个阵列构件(或选定的阵列构件)接触,从而在微流体系统中提供多个化学反应。
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公开(公告)号:US20110118139A1
公开(公告)日:2011-05-19
申请号:US13015242
申请日:2011-01-27
申请人: Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
发明人: Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
CPC分类号: C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要: An array of transportable particle sets is used in a microfluidic device for performing chemical reactions in the microfluidic device. The microfluidic device comprises a main channel and intersecting side channels, the main channel and side channels forming a plurality of intersections. The array of particle sets is disposed in the main channel, and the side channels are coupled to reagents. As the particle sets are transported through the intersections of the main channel and the side channels, reagents are flowed through the side channels into contact with each array member (or selected array members), thereby providing a plurality of chemical reactions in the microfluidic system.
摘要翻译: 在微流体装置中使用可移动颗粒组的阵列,用于在微流体装置中进行化学反应。 微流体装置包括主通道和相交的侧通道,主通道和侧通道形成多个交叉点。 颗粒组的阵列设置在主通道中,并且侧通道与试剂偶联。 当颗粒组通过主通道和侧通道的交叉点传输时,试剂通过侧通道流动与每个阵列构件(或选定的阵列构件)接触,从而在微流体系统中提供多个化学反应。
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公开(公告)号:US20090137413A1
公开(公告)日:2009-05-28
申请号:US11928808
申请日:2007-10-30
申请人: Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
发明人: Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
IPC分类号: C40B30/04
CPC分类号: C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要: Arrays of flowable or fixed particle sets are used in microfluidic systems for performing assays and modifying hydrodynamic flow. Also provided are assays utilizing flowable or fixed particle sets within a microfluidic system, as well as kits, apparatus and integrated systems comprising arrays and array members.
摘要翻译: 可流动或固定的颗粒组的数组用于微流体系统中,用于进行测定和改变流体动力学流动。 还提供了利用微流体系统内的可流动或固定的颗粒组的测定法,以及包括阵列和阵列构件的试剂盒,装置和集成系统。
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公开(公告)号:US06547941B2
公开(公告)日:2003-04-15
申请号:US09920090
申请日:2001-07-31
申请人: Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael J. Kennedy
发明人: Anne R. Kopf-Sill , Andrea W. Chow , Peter C. Jann , Morten J. Jensen , Michael Spaid , Colin B. Kennedy , Michael J. Kennedy
IPC分类号: G01N2726
CPC分类号: G01N21/6452 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00628 , B01J2219/0063 , B01J2219/00659 , B01J2219/00707 , B01L3/5025 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/54 , B01L9/527 , B01L2200/023 , B01L2200/027 , B01L2200/028 , B01L2200/147 , B01L2300/0654 , B01L2300/0816 , B01L2300/0829 , B01L2300/0867 , B01L2300/1822 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , G01N21/05 , G01N21/6428 , G01N21/645 , G01N27/44721 , G01N27/44782 , G01N35/0099 , G01N2021/0346 , G01N2021/6419 , G01N2021/6421
摘要: Analytical systems and methods that use a modular interface structure for providing an interface between a sample substrate and an analytical unit, where the analytical unit typically has a particular interface arrangement for implementing various analytical and control functions. Using a number of variants for each module of the modular interface structure advantageously provides cost effective and efficient ways to perform numerous tests using a particular substrate or class of substrates with a particular analytical and control systems interface arrangement. Improved optical illumination and detection system for simultaneously analyzing reactions or conditions in multiple parallel microchannels are also provided. Increased throughput and improved emissions detection is provided by the present invention by simultaneously illuminating multiple parallel microchannels at a non-normal incidence using an excitation beam including multiple excitation frequencies, and simultaneously detecting emissions from the substances in the microchannels in a direction normal to the substrate using a detection module with multiple detectors.
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