公开(公告)号：US20240075187A1

公开(公告)日：2024-03-07

申请号：US18273611

申请日：2022-05-16

申请人： JIANGSU TRULIVE MEDTECH CO., LTD.

发明人： Wei YANG ,  Jing ZHAO

IPC分类号： A61L27/36

CPC分类号： A61L27/3687 ,  A61L27/3625 ,  A61L2430/20 ,  A61L2430/40

摘要： The present invention discloses a dry biological tissue material and a method of preparing the material. The method includes: performing a decellularization treatment on the biological tissue material using decellularization solutions, wherein the decellularization solutions include first to N-th decellularization solutions with gradually increasing concentrations; performing a first anti-calcification treatment on the biological tissue material using a dissolving solution, wherein the dissolving solution is used to dissolve phospholipid impurities in the biological tissue; performing a second anti-calcification treatment on the biological tissue material using a removal solution, wherein the removal solution is used to remove carboxyl groups produced in the cross-linking and decellularization treatments as a result of oxidation of aldehyde groups; performing a third anti-calcification treatment on the biological tissue material using a reducing solution, wherein the reducing solution is used to protect free aldehyde groups; and performing a dehydration treatment on the biological tissue material using dehydrating solutions, wherein the dehydrating solutions include first to M-th dehydrating solutions with gradually increasing concentrations, and wherein the concentration of the M-th dehydrating solution is lower than the concentration of the N-th decellularization solution. The three anti-calcification treatments respectively for dissolving phospholipids, removing carboxylic acids and protecting aldehyde groups make up a complete anti-calcification process, thereby achieving a high anti-calcification performance.

公开(公告)号：US11896736B2

公开(公告)日：2024-02-13

申请号：US16926905

申请日：2020-07-13

申请人： GLOBUS MEDICAL, INC.

发明人： Joshua Cige ,  Archana Bhat ,  Joanna Marquez

IPC分类号： A61L27/36 ,  A61L27/56

CPC分类号： A61L27/3608 ,  A61L27/365 ,  A61L27/3616 ,  A61L27/3683 ,  A61L27/56 ,  A61L2430/02 ,  A61L2430/40

摘要： Biomaterials, implants made therefrom, methods of making the biomaterial and implants, methods of promoting bone or wound healing in a mammal by administering the biomaterial or implant to the mammal, and kits that include such biomaterials, implants, or components thereof. The biomaterials may include demineralized cortical fibers, which help to promote bone repair.

公开(公告)号：US11890395B2

公开(公告)日：2024-02-06

申请号：US16010120

申请日：2018-06-15

申请人： Avery Therapeutics, Inc.

发明人： Jordan J. Lancaster ,  Steven Goldman ,  Jennifer Watson Koevary

IPC分类号： A61L27/36 ,  A61L27/38 ,  A61L27/58 ,  A61L27/56 ,  A61L27/22 ,  A61L27/34

CPC分类号： A61L27/3633 ,  A61L27/22 ,  A61L27/34 ,  A61L27/3691 ,  A61L27/383 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3821 ,  A61L27/3826 ,  A61L27/3834 ,  A61L27/56 ,  A61L27/58 ,  A61L27/367 ,  A61L27/3873 ,  A61L2300/604 ,  A61L2300/606 ,  A61L2300/64 ,  A61L2420/00 ,  A61L2430/20 ,  A61L2430/40

摘要： Engineered tissue compositions for supporting cell growth, maintenance, and/or differentiation featuring a scaffold, extracellular matrix (ECM) material, and optionally a population of ECM-generating cells such as fibroblasts. The tissue compositions may be used for supporting seeded cells of a particular cell type of interest such as cells related to skeletal muscle, smooth muscle, cardiac tissue, gastrointestinal tissue, etc. The tissue compositions with seeded cells may develop into functional tissues, which may have the potential to provide a tissue graft for therapeutic purposes or a valuable model for in vitro assays.

公开(公告)号：US20240016851A1

公开(公告)日：2024-01-18

申请号：US18372903

申请日：2023-09-26

申请人： Jointechlabs, Inc.

发明人： Nathan Katz ,  Nishit Pancholi

IPC分类号： A61K35/28 ,  A61K35/35 ,  B01L3/00 ,  B33Y10/00 ,  B33Y70/00 ,  B04B3/00 ,  B01D21/00 ,  B01D21/26 ,  A61L27/36

CPC分类号： A61K35/28 ,  A61K35/35 ,  B01L3/5021 ,  B33Y10/00 ,  B33Y70/00 ,  B04B3/00 ,  B01D21/0012 ,  B01D21/262 ,  A61L27/3691 ,  B01L2300/0832 ,  B01L2200/025 ,  B01D2221/10 ,  A61L2430/40 ,  B01L2300/0681

摘要： A device that allows for either fat graft preparation or cell fraction harvest is disclosed. The device includes a first centrifuge tube configured to receive and process a biological substance, the first centrifuge tube comprising an upper cylindrical portion and a lower conical portion, a sterile tissue inlet fitting, at least one sterile processing fluid inlet fitting, a sterile suction fitting, and at least one sterile extraction port connected to a first extraction tube. The first centrifuge tube further includes an internal space including a screen being positioned therein, the screen being configured to divide the internal space in half, and a filter positioned therein, the filter being positioned below the screen in the lower conical portion of the first centrifuge tube. The device may further include a second centrifuge tube configured to receive and further process the biological substance from the first centrifuge tube. The second centrifuge tube has at least one sterile fitting, wherein the second centrifuge tube is releasably connected via the at least one sterile fitting to one of the at least one sterile extraction ports of the first centrifuge tube.

公开(公告)号：US20240002791A1

公开(公告)日：2024-01-04

申请号：US18039849

申请日：2021-12-08

申请人： THE FRANCIS CRICK INSTITUTE LIMITED

发明人： Paola BONFANTI

IPC分类号： C12N5/078 ,  A61L27/38

CPC分类号： C12N5/0634 ,  A61L27/3813 ,  A61L27/3895 ,  A61L2430/40

摘要： The present invention provides a method of producing a thymic construct suitable for implantation into a subject, the method comprising the steps of: (i) providing an acellular scaffold; (ii) seeding the acellular scaffold with thymic epithelial cells having mesenchymal properties; and (iii) culturing the seeded scaffold to produce said construct. The thymic epithelial cells are preferably CD49f+ and may also be VIM+, TE-7+ and/or CD90+. The invention further provides pharmaceutical compositions, uses and therapies using thymic epithelial cells having mesenchymal properties.

公开(公告)号：US20230414831A1

公开(公告)日：2023-12-28

申请号：US18463691

申请日：2023-09-08

申请人： Trustees of Tufts College

发明人： Nicole R. Raia ,  David L. Kaplan ,  Benjamin P. Partlow

IPC分类号： A61L27/26 ,  A61K47/36 ,  A61K47/42 ,  A61K47/46 ,  A61L27/36 ,  A61L27/52 ,  A61L27/54

CPC分类号： A61L27/26 ,  A61K47/36 ,  A61K47/42 ,  A61K47/46 ,  A61L27/3604 ,  A61L27/52 ,  A61L27/54 ,  A61L2430/40 ,  C08L89/00

摘要： In some embodiments, the present disclosure provides compositions including silk fibroin and a phenol-containing polymer, wherein at least one tyrosine group of the silk fibroin is covalently crosslinked to at least one phenol group of the phenol-containing polymer. In some embodiments, the present invention also provides methods including the steps of providing silk fibroin, providing a phenol-containing polymer, associating the silk fibroin with the phenol-containing polymer to form a mixed solution, and crosslinking at least one tyrosine group in the silk fibroin and at least one phenol group of the phenol-containing polymer via at least one enzymatic reaction, wherein the crosslinking comprises covalent bonding between at least one tyrosine group of the silk fibroin and at least one phenol group of the phenol-containing polymer to form a crosslinked composition.

公开(公告)号：US20230398264A1

公开(公告)日：2023-12-14

申请号：US18103102

申请日：2023-01-30

申请人： Fibrin, LLC

发明人： Mark H. Widick ,  Abraham Lavi

IPC分类号： A61L31/04 ,  A61L31/14 ,  C08J9/30 ,  C08J9/00 ,  C08L89/00

CPC分类号： A61L31/046 ,  A61L31/146 ,  C08J9/30 ,  C08J9/0066 ,  C08L89/00 ,  A61L31/047 ,  A61L31/148 ,  C08J2207/10 ,  C08J2389/00 ,  C08L2201/06 ,  C08L2203/02 ,  C08L2203/14 ,  A61L2430/40

摘要： The present invention is a specially prepared fibrin foam, and a method of (and equipment for) making it, which is flexible, contains either open cells, closed cells or both, and having individual cell diameters between 0.001 and 2 mm. Typical ratios of reactants, to give the desired foam characteristics, include 50 cc (45-55 cc) of whole blood (prior to separation to the plasma component) with the subsequent addition thereto of 2 ml (1.5-2.5 ml) 3% hydrogen peroxide, 5000 units (4500-5500 units) thrombin and 1 gm (0.9-1.1 g) calcium chloride in 3 cc (2-4 cc) water. The present invention also includes specialty vessels and constructs, namely, automated, or semi-automated inner containers for the non-blood reactants, and a custom outer separation vessel having a punted based with an annular base lip as well as an upper tube shape tapering inward towards its top annular opening.

公开(公告)号：US20230381373A1

公开(公告)日：2023-11-30

申请号：US18317526

申请日：2023-05-15

申请人： Yale University

发明人： Michael Mak ,  Xiangyu Gong

IPC分类号： A61L27/24 ,  A61L27/26 ,  A61L27/50

CPC分类号： A61L27/24 ,  A61L27/26 ,  A61L27/507 ,  A61L2430/20 ,  A61L2430/40

摘要： In some aspects, the present invention provides a method of forming crowded collagen based constructs, having the steps of providing a collagen solution in a buffer, providing a macromolecular bath solution, and delivering the collagen solution into the macromolecular bath solution to form crowded collagen constructs. In other aspects, the present invention provides a method of forming crowded cellular based constructs, having the steps of providing a cellular solution in a buffer, providing a macromolecular bath solution, and delivering the cellular solution into the macromolecular bath solution to form crowded cellular constructs.

公开(公告)号：US20230330306A1

公开(公告)日：2023-10-19

申请号：US18211416

申请日：2023-06-19

申请人： VENUS MEDTECH (HANGZHOU) INC.

发明人： Zhaogang Liu ,  Yuting Zhu ,  Dajun Kuang ,  Hou-Sen Lim

IPC分类号： A61L27/36 ,  A61L27/54

CPC分类号： A61L27/54 ,  A61L27/3625 ,  A61L27/3687 ,  A61L2300/414 ,  A61L2430/20 ,  A61L2430/40

摘要： Provided are a pro-endothelialization biological material and method for preparation thereof, and a pro-endothelialization heart valve and method for preparation thereof and interventional system. The pro-endothelialization biological material comprises a biological material and a pro-endothelializing growth factor loaded on the surface of biological material; capable of capturing endothelial progenitor cells, enabling the endothelial progenitor cells to attach, grow, and differentiate on the surface of the biological material. The methods for preparation of the pro-endothelialization biological material comprises chemical grafting and physical coating methods. The heart valve can be prepared either by suturing followed by grafting or by grafting followed by suturing. A endothelial growth factor is directly loaded onto the surface of a biological material film, promoting the formation of endothelial cell layers on the surface of the biological material.

公开(公告)号：US11752174B2

公开(公告)日：2023-09-12

申请号：US17120626

申请日：2020-12-14

申请人： MiMedx Group, Inc.

发明人： John Daniel ,  Randall Spencer ,  John Russo ,  Robert Tofe

IPC分类号： A61K35/28 ,  A61L27/36 ,  A61L27/38 ,  A61K35/50 ,  A61K35/33 ,  A61K35/19 ,  A61L27/56

CPC分类号： A61K35/28 ,  A61K35/19 ,  A61K35/33 ,  A61K35/50 ,  A61L27/3604 ,  A61L27/3683 ,  A61L27/3834 ,  A61L27/56 ,  A61F2210/00 ,  A61F2230/00 ,  A61L2430/34 ,  A61L2430/40 ,  Y10T156/10 ,  Y10T156/1039

摘要： Described herein are tissue grafts derived from the placenta. The grafts are composed of at least one layer of amnion tissue where the epithelium layer has been substantially removed in order to expose the basement layer to host cells. By removing the epithelium layer, cells from the host can more readily interact with the cell-adhesion bio-active factors located onto top and within of the basement membrane. Also described herein are methods for making and using the tissue grafts. The laminin structure of amnion tissue is nearly identical to that of native human tissue such as, for example, oral mucosa tissue. This includes high level of laminin-5, a cell adhesion bio-active factor show to bind gingival epithelia-cells, found throughout upper portions of the basement membrane.