公开(公告)号：US11952590B2

公开(公告)日：2024-04-09

申请号：US18329469

申请日：2023-06-05

申请人： Orbsen Therapeutics Limited

发明人： Stephen J. Elliman

IPC分类号： C12N5/0775 ,  A61K9/00 ,  A61K35/28 ,  A61K35/407 ,  C12N5/074 ,  C12N5/077 ,  C12Q1/37 ,  G01N33/50

CPC分类号： C12N5/0662 ,  A61K9/0019 ,  A61K35/28 ,  A61K35/407 ,  C12N5/0607 ,  C12N5/0663 ,  C12N5/0669 ,  C12Q1/37 ,  G01N33/5005

摘要： Stromal stem cells are prospectively isolated from human bone marrow then expanded into clonal populations and cultured and used, the isolation being on the basis of expression of a cell surface marker, wherein the cell surface marker binds an antibody and wherein said antibody cross reacts with a cell surface marker found on mouse stromal stem cells or rat stromal stem cells, and optionally also on a cell of at least one other mammalian species selected from mouse, rat, horse, rabbit and pig cells. Useful stromal stem cell populations are positive for SDC2.

公开(公告)号：US20240101967A1

公开(公告)日：2024-03-28

申请号：US18266093

申请日：2021-12-10

申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： George M. Church ,  Eriona Hysolli ,  Jessica Weber ,  Pranam Chatterjee ,  Cory Smith

IPC分类号： C12N5/074 ,  C12N5/0775 ,  C12N9/22 ,  C12N15/11

CPC分类号： C12N5/0696 ,  C12N5/0662 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20 ,  C12N2510/00

摘要： Described herein are compositions and methods for generating a viable cell that expresses at least one or more woolly mammoth genes. Also described herein are compositions and methods for generating an embryo, blastula, oocyte, or non-human organism that expresses one or more woolly mammoth genes.

公开(公告)号：US20240101961A1

公开(公告)日：2024-03-28

申请号：US18521129

申请日：2023-11-28

申请人： Mesoblast, Inc.

发明人： Stan Gronthos ,  Andrew Christopher William Zannettino

IPC分类号： C12N5/0775 ,  A61K35/28 ,  A61K38/18 ,  A61K38/19 ,  A61K38/20 ,  A61L27/38 ,  C12N5/0735

CPC分类号： C12N5/0663 ,  A61K35/28 ,  A61K38/1858 ,  A61K38/1875 ,  A61K38/191 ,  A61K38/195 ,  A61K38/2006 ,  A61L27/3804 ,  A61L27/3834 ,  A61L27/3839 ,  A61L27/3886 ,  A61L27/3895 ,  C12N5/0606 ,  C12N5/0662 ,  C12N5/0664 ,  C12N5/0665 ,  C12N5/0666 ,  C12N5/0667 ,  C12N5/0668 ,  C12N2510/00

摘要： The invention relates to multipotential expanded mesenchymal precursor progeny (MEMP's), characterised by the early developmental markers STRO-1bri and ALP. The present invention also relates to methods for producing MEMP's and to uses of MEMP's for therapeutic applications.

公开(公告)号：US20240100100A1

公开(公告)日：2024-03-28

申请号：US18357639

申请日：2023-07-24

申请人： Beijng Jiyuan Biological Technology Co., Ltd.

发明人： Haifeng DUAN ,  Binghua XUE ,  Jing XIE ,  Zhenli ZHANG

IPC分类号： A61K35/28 ,  C07K14/50 ,  C07K14/605 ,  C12N5/0775

CPC分类号： A61K35/28 ,  C07K14/50 ,  C07K14/605 ,  C12N5/0662 ,  A61K38/00

摘要： Provided are a modified mesenchymal stem cell and culture supernatant thereof, and a pharmaceutical composition comprising said cell or culture supernatant thereof. The mesenchymal stem cell is capable of expressing: (1) a first protein, which is selected from FGF21 or a variant thereof, or a first fusion protein comprising the FGF21 or the variant thereof and (2) a second protein, which is selected from GLP-1 or a variant thereof or a second fusion protein comprising the GLP-1 or the variant thereof. Also provided is the use of the modified mesenchymal stem cell and culture supernatant thereof, and the pharmaceutical composition comprising said cell or culture supernatant thereof in the treatment of metabolic diseases and in the preparation of a medicament for treating metabolic diseases.

公开(公告)号：US11926848B2

公开(公告)日：2024-03-12

申请号：US17470492

申请日：2021-09-09

申请人： Orbsen Therapeutics Limited

发明人： Stephen J. Elliman

IPC分类号： C12N5/0775 ,  A61K9/00 ,  A61K35/28 ,  A61K35/407 ,  C12N5/074 ,  C12N5/077 ,  C12Q1/37 ,  G01N33/50

CPC分类号： C12N5/0662 ,  A61K9/0019 ,  A61K35/28 ,  A61K35/407 ,  C12N5/0607 ,  C12N5/0663 ,  C12N5/0669 ,  C12Q1/37 ,  G01N33/5005

摘要： Stromal stem cells are prospectively isolated from human bone marrow then expanded into clonal populations and cultured and used, the isolation being on the basis of expression of a cell surface marker, wherein the cell surface marker binds an antibody and wherein said antibody cross reacts with a cell surface marker found on mouse stromal stem cells or rat stromal stem cells, and optionally also on a cell of at least one other mammalian species selected from mouse, rat, horse, rabbit and pig cells. Useful stromal stem cell populations are positive for SDC2.

公开(公告)号：US11891620B2

公开(公告)日：2024-02-06

申请号：US14712491

申请日：2015-05-14

申请人： Mayo Foundation for Medical Education and Research

发明人： Allan B. Dietz ,  Gaylord Knutson

IPC分类号： C12N5/00 ,  C12N5/0775 ,  C12N5/071

CPC分类号： C12N5/0037 ,  C12N5/0068 ,  C12N5/069 ,  C12N5/0662 ,  C12N5/0692 ,  C12N2500/84 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/165 ,  C12N2502/115 ,  C12N2533/50 ,  C12N2533/90

摘要： Platelet lysate compositions and cell culture media compositions for maintaining and/or growing mammalian cells, such as mammalian endothelial cells (ECs) and mammalian endothelial progenitor cells (EPCs), in particular human ECs (huECs) and human EPCs (huEPCs), such as primary huECs and primary huEPCs, are provided. The cell culture media compositions contain a basal medium, a platelet lysate and, optionally, one or more exogenously added growth factors. Also provided are methods for making and using such cell culture media compositions to grow and/or maintain ECs and EPCs, including huECs and huEPCs, as well as cell culture vessels, dishes, plates, and/or flasks pretreated with the cell culture media compositions.

公开(公告)号：US20230371498A1

公开(公告)日：2023-11-23

申请号：US17748041

申请日：2022-05-19

申请人： Zhe Li

发明人： Zhe Li ,  Xi Li

IPC分类号： A01N1/02 ,  C12N5/0775 ,  A61P17/02 ,  A61K38/18 ,  A61K35/12 ,  A61K9/00

CPC分类号： A01N1/0221 ,  C12N5/0662 ,  A61P17/02 ,  A61K38/18 ,  A61K35/12 ,  A61K9/0014

摘要： A micro frozen body for loading drugs, the micro frozen body is formed of frozen liquid, the micro frozen body contains drugs, the micro frozen body releases drugs to the target tissue in the organism. The liquid is solidified by cryogenic freezing to form a solid-state micro frozen body, the drug loaded in micro freezing or the micro frozen body itself is formed of solidified drug solution, the micro frozen body can be made into a needle with certain hardness, the micro frozen body implements puncture, the low temperature of micro frozen body can reduce the aching feeling in the piercing process and constrict the capillary vessels on the site of puncture to reduce bleeding.

公开(公告)号：US11821004B2

公开(公告)日：2023-11-21

申请号：US17087124

申请日：2020-11-02

申请人： Mesoblast International Sárl

发明人： Alla Danilkovitch ,  Diane Carter ,  Alicia Mildred Tyrell ,  Simon Bubnic ,  Michelle Marcelino ,  Rodney Monroy

IPC分类号： A01N63/00 ,  G01N33/53 ,  C12N5/00 ,  C12N5/0775 ,  A61K35/28 ,  A01K67/00 ,  A61K35/12

CPC分类号： C12N5/0662 ,  A61K35/28 ,  C12N5/0663 ,  A61K2035/122 ,  C12N2501/25 ,  G01N2333/525

摘要： Mesenchymal stem cells which express TNF-α receptor Type I in an amount of at least 13 pg/106 cells. Such mesenchymal stem cells inhibit the proliferation of lymphocytes and may be employed, in particular, in the treatment of graft-versus-host disease.

公开(公告)号：US11806368B2

公开(公告)日：2023-11-07

申请号：US16875486

申请日：2020-05-15

申请人： Agency for Science, Technology and Research

发明人： Lawrence Stanton ,  Padmapriya Sathiyanathan ,  Simon Cool ,  Victor Nurcombe ,  Rebekah M. Samsonraj

IPC分类号： A61K35/28 ,  C12Q1/6881 ,  A61K35/545 ,  C12N5/0775

CPC分类号： A61K35/28 ,  A61K35/545 ,  C12N5/0662 ,  C12N5/0663 ,  C12Q1/6881 ,  C12Y205/01018 ,  C12Q2600/158

摘要： A method for providing a sub-population of stem cell or plurality of stem cells by determining or modulating GSTT1 expression level or genotype is disclosed together with uses of the stem cells.

公开(公告)号：US20230323304A1

公开(公告)日：2023-10-12

申请号：US18314411

申请日：2023-05-09

申请人： INSTITUT PASTEUR ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)

发明人： James DI SANTO ,  Ai Ing LIM

IPC分类号： G01N33/50 ,  G01N15/14 ,  C12N5/0775 ,  A61K49/00 ,  C12N5/0789 ,  G01N33/68

CPC分类号： C12N5/0647 ,  A61K49/0008 ,  C12N5/0662 ,  G01N15/14 ,  G01N33/5047 ,  G01N33/5094 ,  G01N33/6869 ,  C12N2501/2301 ,  C12N2501/2302 ,  C12N2501/2307

摘要： Innate lymphoid cells (ILCs) represent innate versions of T helper and cytotoxic T cells that differentiate from committed ILC precursors (ILCP). Still, how ILCP relate to mature tissue-resident ILCs remains unclear. ILCP that are present in the blood and all tested lymphoid and non-lymphoid human tissues were identified. Human ILCP fail to express the signature transcription factors (TF) and cytokine outputs of mature NK cells and ILCs but are epigenetically poised to do so. Human ILCP robustly generate all ILC subsets in vitro and in vivo. While human ILCP express RAR related orphan receptor C (RORC), circulating ILCP can be found in RORC-deficient patients that retain potential for EOMES+ NK cells, T-BET+ ILC1, GATA-3+ ILC2 and for IL-22+ but not for IL-17A+ ILC3. A model of tissue ILC differentiation (‘ILC-poiesis’) is proposed whereby diverse ILC subsets are generated in situ from ILCP in response to environmental stressors, inflammation and infection.