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    DETECTION OF TARGET NUCLEIC ACID SEQUENCES USING DUAL-LABELED IMMOBILIZED PROBES ON SOLID PHASE
    13.
    发明申请
    DETECTION OF TARGET NUCLEIC ACID SEQUENCES USING DUAL-LABELED IMMOBILIZED PROBES ON SOLID PHASE 有权
    使用双标准固定化探针在固相上检测目标核酸序列

    公开(公告)号：US20130252827A1

    公开(公告)日：2013-09-26

    申请号：US13880199

    申请日：2011-10-20

    申请人： Jong Yoon Chun

    发明人： Jong Yoon Chun

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6837 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2565/1015 ,  C12Q2521/319

    摘要： The present invention relates to a novel method for detection of target nucleic acid sequences on a solid phase using dual-labeled immobilized probes and its resistance to a 5′ to 3′ exonuclease activity of a DNA polymerase. Because the label is remained on the solid substrate by resistance to nucleases due to labeling of a base component the internal nucleotide, the present invention requires no consideration of a suitability of position of the label for remaining on the solid substrate. The present invention ensures to minimize background signal by positioning labels at a site on probes suitable to maximize quenching efficiency of the dual label system, since it permits to freely determine the position of the internal label on probes.

    摘要翻译： 本发明涉及使用双标记固定化探针在固相上检测靶核酸序列及其对DNA聚合酶的5'至3'核酸外切酶活性的抗性的新方法。 因为由于碱性成分的内标核苷酸的标记，由于对核酸酶的耐受性，标记物保留在固体基质上，所以本发明不需要考虑用于残留在固体基质上的标记的位置的适合性。 本发明通过将标签定位在适于最大化双标签系统的淬火效率的探针上的位置上，从而确保了背景信号的最小化，因为它允许自由地确定探针上的内部标签的位置。

    MICROARRAY SYSTEM WITH IMPROVED SEQUENCE SPECIFICITY
    14.
    发明申请
    MICROARRAY SYSTEM WITH IMPROVED SEQUENCE SPECIFICITY 审中-公开

    公开(公告)号：US20130040860A1

    公开(公告)日：2013-02-14

    申请号：US13650087

    申请日：2012-10-11

    申请人： Kerry B. Gunning ,  Mark Aaron Behike

    发明人： Kerry B. Gunning ,  Mark Aaron Behike

    IPC分类号： C40B50/18 ,  C40B40/06

    CPC分类号： C12Q1/6837 ,  B01J19/0046 ,  B01J2219/0059 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00722 ,  C12P19/34 ,  C12Q1/6806 ,  Y10T436/108331 ,  C12Q2525/125 ,  C12Q2533/101 ,  C12Q2521/319

    摘要： The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.

    Composition and method for sequencing nucleic acid
    16.
    发明授权
    Composition and method for sequencing nucleic acid 有权
    核酸测序的组成和方法

    公开(公告)号：US08211673B2

    公开(公告)日：2012-07-03

    申请号：US12365140

    申请日：2009-02-03

    申请人： Linda G. Lee

    发明人： Linda G. Lee

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/101

    摘要： A composition for sequencing DNA is provided and comprises a nuclease and a nuclease-resistant sequencing primer. A method of preparing DNA for sequencing and a method of sequencing DNA are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the nuclease-resistant sequencing primer is essentially non-degraded.

    摘要翻译： 提供了用于测序DNA的组合物，并且包含核酸酶和核酸酶抗性测序引物。 还提供了制备用于测序的DNA的方法和测序DNA的方法。 测序DNA的方法可以包括在扩增引物被核酸酶降解的条件下将扩增反应产物与组合物接触，并且核酸酶抗性测序引物基本上是未降解的。

    Selective 5′ ligation tagging of RNA
    17.
    发明授权
    Selective 5′ ligation tagging of RNA 有权
    RNA的选择性5'连接标记

    公开(公告)号：US08163491B2

    公开(公告)日：2012-04-24

    申请号：US12707243

    申请日：2010-02-17

    申请人： Jerome Jendrisak ,  Ramesh Vaidyanathan ,  Gary Dahl

    发明人： Jerome Jendrisak ,  Ramesh Vaidyanathan ,  Gary Dahl

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12N15/1096 ,  C12Q1/6855 ,  C12Q2525/207 ,  C12Q2525/125 ,  C12Q2521/525

    摘要： The present invention provides novel compositions, kits and methods employing RNA 5′ polyphosphatases, RNA 5′ monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5′ ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5′ ends. The 5′tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.

    摘要翻译： 本发明提供使用RNA 5'多磷酸酶，RNA 5'单磷酸酶，封端酶，脱蛋白酶，核酸焦磷酸酶和RNA连接酶以及其它酶的新型组合物，试剂盒和方法，用于选择性5'连接标记所需类别的 相对于其5'端的特定化学部分不同的RNA分子。 5'标记的RNA分子可用于合成标记的第一支cDNA，双链cDNA和用于各种用途的正义或反义RNA。