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    Methods of identifying multiple epitopes in cells
    12.
    发明授权
    Methods of identifying multiple epitopes in cells 有权

    公开(公告)号：US12110536B2

    公开(公告)日：2024-10-08

    申请号：US18630941

    申请日：2024-04-09

    申请人： Roche Sequencing Solutions, Inc.

    发明人： Garry P. Nolan

    IPC分类号： C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/686

    CPC分类号： C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/686

    摘要： A method of barcoding is provided. The method comprises: (a) obtaining a population of cells or cell organelles in a first volume, wherein the cells or cell organelles comprise target molecules that are associated with a first assayable oligonucleotide subunit; (b) separating the cells or cell organelles into a plurality of second volumes, wherein at least some of second volumes receive a single cell or cell organelle from the population of cells or cell organelles; and (c) associating a plurality of second assayable oligonucleotide subunits with the first assayable oligonucleotide subunit in the second volumes, wherein at least some of the second volumes each receive a different second assayable oligonucleotide subunit, to produce at least some nucleic acid molecules that comprise a first assayable oligonucleotide subunit and a second assayable oligonucleotide subunit.

    SINGLE CELL CHROMATIN IMMUNOPRECIPITATION SEQUENCING ASSAY
    14.
    发明公开
    SINGLE CELL CHROMATIN IMMUNOPRECIPITATION SEQUENCING ASSAY 审中-公开

    公开(公告)号：US20240327897A1

    公开(公告)日：2024-10-03

    申请号：US18589293

    申请日：2024-02-27

    申请人： Becton, Dickinson and Company

    发明人： Hye-Won Song

    IPC分类号： C12Q1/6806 ,  C12N9/22 ,  C12Q1/68 ,  C12Q1/6874

    CPC分类号： C12Q1/6806 ,  C12N9/22 ,  C12Q1/68 ,  C12Q1/6874 ,  C12Q2565/1025 ,  C12Q2600/154

    摘要： Disclosed herein include systems, methods, kits, and compositions for labeling nuclear target-associated DNA in a cell. Some embodiments provide digestion compositions comprising a DNA digestion enzyme and a binding reagent capable of specifically binding to the nuclear target. Some embodiments provide conjugates comprising a transposome and a binding reagent capable of specifically binding to a nuclear target. The transposome can comprise a transposase (e.g., Tn5 transposase), a first adaptor having a first 5′ overhang, and a second adaptor having a second 5′ overhang. The methods can comprise contacting a permeabilized cell comprising a nuclear target associated with dsDNA, such as genomic DNA (gDNA), with the compositions provided herein to generate a plurality of nuclear target-associated dsDNA fragments (e.g., nuclear target-associated gDNA fragments) each comprising the one or two single-stranded overhangs.

    METHODS AND REAGENTS FOR ANALYZING NUCLEIC ACIDS
    20.
    发明公开
    METHODS AND REAGENTS FOR ANALYZING NUCLEIC ACIDS 审中-公开

    公开(公告)号：US20240318162A1

    公开(公告)日：2024-09-26

    申请号：US18575659

    申请日：2022-06-30

    申请人： CUBIT DIAGNOSTICS, INC.

    发明人： Samuel J. Hartman-Pickerill ,  Martine Dominique Lunke ,  Matthew Dean McFarlane ,  Jackson William Price ,  Paul John Romaniuk

    IPC分类号： C12N15/10 ,  C12Q1/6806

    CPC分类号： C12N15/1006 ,  C12Q1/6806

    摘要： Methods of specifically capturing and processing nucleic acids from samples in a simplified, streamlined workflow. Media conditions and/or workflow improvements provided herein permit workflow steps such as cell lysis, nucleic acid denaturation, and target nucleic acid capture and immobilization to be performed in a “one-pot” reaction with minimal or no subsequent washing or rinsing steps prior to enzymatic processing. The media conditions include particular salt and/or detergent concentrations, and the workflow improvements include improved purification steps. The media conditions and workflow improvements effectively isolate target nucleic acid for downstream enzymatic processing reactions while avoiding levels of contaminants that inhibit such reactions.