公开(公告)号：US20140042027A1

公开(公告)日：2014-02-13

申请号：US13961626

申请日：2013-08-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Christopher Belisle

IPC: G01N27/447

CPC classification number: G01N27/44747 ,  G01N27/44713 ,  G01N27/44721 ,  G01N27/44726 ,  G01N27/44739

Abstract: Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.

Abstract translation: 在凝胶中电泳分离的蛋白质通过将卤素取代的有机化合物引入凝胶两端的一个或两个电极缓冲溶液中衍生而产生荧光发射。 使用的卤素取代的化合物是在缓冲溶液和凝胶的pH下具有电荷的化合物，并且化合物上的电荷的极性使得化合物在电泳影响下从电极缓冲液迁移到凝胶中 随着蛋白质迁移到凝胶中。 一旦蛋白质被分离并分布在凝胶中并且凝胶被卤素取代的化合物完全渗透，则用紫外线照射凝胶以通过蛋白质上的色氨酸残基诱导卤素取代的化合物与蛋白质之间的反应 ，产生荧光反应产物。

公开(公告)号：US20140038237A1

公开(公告)日：2014-02-06

申请号：US14058044

申请日：2013-10-18

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： YAN WANG

IPC: C12P19/34 ,  C07K14/00

CPC classification number: C12N9/1276 ,  C07K14/00 ,  C07K14/001 ,  C07K19/00 ,  C07K2319/80 ,  C12N9/1252 ,  C12N9/96 ,  C12P19/34 ,  C12Y207/07007 ,  C12Y207/07049 ,  Y02P20/52

Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.

Abstract translation: 本发明提供了改进的新型核酸修饰酶的产生。 改进是以提高酶结合和催化修饰核酸的能力的方式将序列非特异性核酸结合结构域与酶的融合。

公开(公告)号：US20130340539A1

公开(公告)日：2013-12-26

申请号：US13923480

申请日：2013-06-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nathan Michael Gaskill-Fox ,  Daniel N. Fox ,  Rodney C. Harris

IPC: B01L9/00

CPC classification number: B01L9/52 ,  B01L9/06 ,  G01N15/14 ,  G01N35/1011 ,  G01N2015/149

Abstract: Disclosed is a collection sled for collecting a waste droplet stream 122 in a waste stream catcher that is located below sample collectors. Contamination of collected samples is reduced by collecting the waste droplet stream at a position that is below the sample collectors. A waste trough is provided that gets progressively larger in a downward direction, which prevents backsplash of waste fluid from the collection cavity. Standard sample collector tubes, as well as 8-well strips and microscope slides can be used with the collection sled to collect sample particles.

Abstract translation: 公开了一种用于在位于样品收集器下方的废物流捕集器中收集废液滴流122的收集筒。 通过在低于样品收集器的位置处收集废液滴流来减少收集的样品的污染。 提供了在向下方向逐渐变大的废物槽，其防止废液从收集腔中脱落。 标准样品收集器管以及8孔条和显微镜载玻片可与收集雪橇一起使用以收集样品颗粒。

公开(公告)号：US20130330230A1

公开(公告)日：2013-12-12

申请号：US13911115

申请日：2013-06-06

Applicant: Bio-Rad Laboratories Inc.

Inventor： Yochanan URI ,  Boaz Ran ,  Ariel G. Notcovich ,  Shay Nimri

IPC: G01N21/65

CPC classification number: G01N21/553 ,  G01N21/253 ,  G01N21/658 ,  G01N33/54373 ,  G01N2021/0328 ,  G01N2201/0407 ,  G01N2201/0484 ,  G01N2201/062

Abstract: A tip for use in an optical detection system to analyze an analyte in a fluid sample drawn into the tip, using light reflected from a detection surface inside the tip that the analyte binds to, comprising a first detection surface and a second detection surface located in a same flow path with no controllable valve separating them, wherein the first and second detection surfaces have different surface chemistries.

Abstract translation: 一种用于光学检测系统的尖端，用于使用从分析物结合到所述尖端内部的检测表面反射的光来分析吸入所述尖端的流体样品中的分析物，所述检测表面包括第一检测表面和位于 相同的流动路径，没有可分离它们的可控阀，其中第一和第二检测表面具有不同的表面化学性质。

公开(公告)号：US20130306854A1

公开(公告)日：2013-11-21

申请号：US13896572

申请日：2013-05-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Christopher Belisle

IPC: G01N27/447 ,  G01N30/06 ,  H01J49/00

CPC classification number: G01N27/44721 ,  G01N30/06 ,  G01N33/6848 ,  H01J49/0036 ,  H01J49/26

Abstract: Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment.

Abstract translation: 本文公开了一种制备用于质谱法的蛋白质样品的方法。 该方法包括在电泳凝胶上分离样品的蛋白质; 使蛋白质与卤素取代的有机化合物接触; 将凝胶暴露于紫外光下; 检测从电泳凝胶发出的荧光; 基于检测到的荧光来切割至少一部分电泳凝胶，其中所述至少一部分含有蛋白质样品的蛋白质; 并将来自至少一部分的蛋白质进行质谱分析。 使用这种方法，可以通过GeLC-MS鉴定更多的蛋白质，而不是用蛋白质染色剂处理电泳凝胶，或者进行伴随这种处理的凝胶处理步骤。

公开(公告)号：US20130288274A1

公开(公告)日：2013-10-31

申请号：US13692938

申请日：2012-12-03

Applicant: Bio Rad Laboratories, Inc.

Inventor： ROGER WALKER ,  Yabin Lu ,  Urvee Desai ,  Daming Shan

IPC: G01N33/68

CPC classification number: G01N33/6893 ,  C07K14/415 ,  C07K2319/21 ,  C07K2319/23 ,  C12N9/1044 ,  C12Y203/02013 ,  G01N33/564 ,  G01N2800/06

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

Abstract translation: 本发明提供了一种用于通过使来自受试者的体液样品与固定在固体支持物上的麦醇溶蛋白融合蛋白的六聚体形成的抗原来确定受试者是否患有乳糜泻的方法。 抗原的麦醇溶蛋白融合蛋白包括与诸如谷胱甘肽-S转移酶（GST）蛋白质的标签连接的重组脱酰胺麦醇溶蛋白。 通过将麦醇溶蛋白融合蛋白固定在固体支持物上来制备抗原。 抗原可以进一步包括与谷氨酸蛋白融合蛋白交联的组织转谷氨酰胺酶（tTG）。 当存在tTG时，将tTG和重组脱酰基麦醇溶蛋白在固定固相之前混合在一起。

公开(公告)号：US20130270287A1

公开(公告)日：2013-10-17

申请号：US13861143

申请日：2013-04-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kun Guo ,  Paul J. Patt

IPC: G06F17/00

CPC classification number: G06F17/00 ,  B01L3/0289 ,  B01L2200/0652 ,  B01L2200/0657 ,  B01L2200/143 ,  B01L2400/0439 ,  B01L2400/0478 ,  B01L2400/0481 ,  G01N15/02 ,  G01N15/0205 ,  G01N2015/1062 ,  G01N2015/1486

Abstract: Particles are dispensed under controlled conditions to achieve an accurate number of particles by passing a suspension of the particles through a particle detecting device, obtaining a cumulative particle count, comparing the cumulative particle count with a target value, and shutting off the suspension flow once the particle count reaches the target value, all performed by automated means.

Abstract translation: 在受控条件下分配颗粒以通过使颗粒的悬浮液通过颗粒检测装置获得精确数量的颗粒，获得累积颗粒数，将累积颗粒数与目标值进行比较，并一旦关闭悬浮液流 粒子数达到目标值，全部以自动方式进行。

公开(公告)号：US20130267690A1

公开(公告)日：2013-10-10

申请号：US13622093

申请日：2012-09-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Peter S. Gagnon

IPC: C07K1/18

CPC classification number: C07K1/18 ,  A61L2/0088 ,  B01D15/3847 ,  C07K1/20 ,  C07K16/065 ,  C07K2317/52

Abstract: Methods, compositions and kits for chromatography purification of antibodies are provided. In some embodiments, antibodies are purified by hydroxyapatite (HT) or fluorapatite (FT) that is treated with a polycationic agent. In some embodiments, the antibodies are treated with a polycationic agent that is also a virucidal agent prior to purification.

Abstract translation: 提供了用于抗体的色谱纯化的方法，组合物和试剂盒。 在一些实施方案中，抗体通过用聚阳离子剂处理的羟基磷灰石（HT）或氟磷灰石（FT）进行纯化。 在一些实施方案中，抗体在纯化之前用也是杀病毒剂的聚阳离子剂处理。

公开(公告)号：US20130231462A1

公开(公告)日：2013-09-05

申请号：US13776403

申请日：2013-02-25

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Steve Roth ,  William Strong

IPC: C07K16/42

CPC classification number: C07K16/42 ,  C07K2317/31 ,  C07K2317/32

Abstract: Provided are methods and compositions for immunoassays with improved sensitivity and specificity. The presently disclosed anti-immune complex antibodies stabilize the interaction between a primary and secondary antibody, thereby allowing for more stringent wash conditions, less background, and stronger signal.

Abstract translation: 提供了具有改善的灵敏度和特异性的免疫测定法的方法和组合物。 目前公开的抗免疫复合物抗体稳定了一级和二级抗体之间的相互作用，从而允许更严格的洗涤条件，更少的背景和更强的信号。

公开(公告)号：US20130203172A1

公开(公告)日：2013-08-08

申请号：US13719828

申请日：2012-12-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Hannah Wex ,  Matthew Pearce ,  Richard Hall ,  Jake Hodgson

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L3/523 ,  B01L2200/16 ,  B01L2300/045 ,  B01L2300/049 ,  B01L2300/0672 ,  B01L2300/069 ,  B01L2300/0832 ,  B01L2300/0867 ,  B01L2400/0457 ,  B01L2400/0644 ,  B01L2400/0683 ,  Y10T436/11

Abstract: A single closed and compartmentalized vessel is designed to contain all assay reagents necessary to conduct an assay of a liquid sample. The vessel is constructed in parts that are rotatable relative to each other, and processing steps of the assay such as combining, mixing, separating, and measuring are all conducted by either rotating the parts relative to each other or rotating the vessel as a whole, all without opening the receptacle or otherwise exposing the user to any of the liquids or substances within the receptacle.

Abstract translation: 单个封闭和分隔的容器设计成包含进行液体样品测定所必需的所有测定试剂。 容器构造成能够相对于彼此旋转的部分，并且分析的结合，混合，分离和测量的处理步骤都是通过相对于彼此旋转部件或者使整个容器旋转而进行的， 所有这些都不打开容器或以其他方式将使用者暴露在容器内的任何液体或物质中。