公开(公告)号：US20250101391A1

公开(公告)日：2025-03-27

申请号：US18974072

申请日：2024-12-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12 ,  C12N1/20 ,  C12R1/01 ,  C40B40/06

Abstract: Hybrid reverse transcriptases are provided that comprise a non-retroviral retrotransposon, or a fragment of the non-retroviral retrotransposon having reverse transcriptase activity, joined to a nucleic acid binding protein. Also provided are methods of using the hybrid reverse transcriptases to prepare a cDNA molecule library.

公开(公告)号：US12241116B2

公开(公告)日：2025-03-04

申请号：US18536935

申请日：2023-12-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US12239987B2

公开(公告)日：2025-03-04

申请号：US17986873

申请日：2022-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. Stumbo ,  George Carman ,  Steve Hobbs ,  Anthony J. Makarewicz, Jr. ,  Dmitri Simonian ,  David Glade ,  Joshua Oen ,  Denis Pristinski ,  John Dzenitis

IPC: B01L3/00 ,  B01F23/41 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N15/14 ,  G01N15/1429 ,  G01N15/1434

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US20250050346A1

公开(公告)日：2025-02-13

申请号：US18927689

申请日：2024-10-25

Applicant: Bio-Rad Laboratories, Inc. ,  Bio-Rad Europe GmbH

Inventor： Kalyan Handique ,  Ronald Lebofsky ,  Emilie Celine Claudie Macke ,  Rebecca Dievart ,  Christophe Quiring ,  Sophie Pierre

IPC: B01L3/00

Abstract: A system, method, and platform for target detection, the system including: a base substrate; a set of sample processing regions defined at a broad surface of the substrate, wherein each of the set of sample processing regions includes: a set of microwell subarrays arranged in a gradient between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions; and a cover substrate configured to mate with the base substrate in a coupled mode, the cover substrate comprising a network of venting channels aligned with the set of sample processing regions upon mating the base substrate with the cover substrate in the coupled mode, the network of venting channels providing gas exchange between the base substrate and an environment surrounding the microwell assembly. The invention(s) can be used for MPN assays.

公开(公告)号：US12221633B2

公开(公告)日：2025-02-11

申请号：US18348544

申请日：2023-07-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Arkadiusz Bibillo ,  Pranav Patel

IPC: C12N9/12

Abstract: The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. The present disclosure provides methods for preparing cDNA and/or DNA molecules and cDNA and/or DNA libraries using modified reverse transcriptases.

公开(公告)号：US12209277B2

公开(公告)日：2025-01-28

申请号：US16260475

申请日：2019-01-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Sean Cater ,  Lucas Frenz ,  Andrew Kohlway ,  Jennifer Chew ,  Rob Ray ,  Ronald Lebofsky

IPC: C12Q1/6853 ,  C12N15/10 ,  C12Q1/6806

Abstract: Methods are provided of assessing whether multiple differently-barcoded primers occur within the same partition.

公开(公告)号：US12188931B2

公开(公告)日：2025-01-07

申请号：US17562055

申请日：2021-12-27

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Michelle Delanoy ,  John Flanagan ,  Audrey Arjomandi ,  Ravi Kaul ,  Steven R. Binder

IPC: C07K14/20 ,  A61K39/02 ,  G01N33/569

Abstract: The present invention relates to an immunoassay for the detection of Borrelia specific IgG, IgM and IgG/IgM antibodies in biological samples suspected of Lyme infection. The immunoassay can be performed via a standard immunoassay format or on an automated platform. In various embodiments, the immunoassay uses one or more Borrelia specific chimeric peptides VlsE-FlaB (designated pFlaB-mV), VlsE-ErpP (designated pErp59-mV), VlsE-P35 (designated pP35-mV) alone or in combination with one or more outer surface protein C (Osp C) types B or I, p58 and DbpA. Other aspects of the invention provide antigen/substrate combinations and compositions comprising combinations of the disclosed peptides and/or proteins for use in the immunoassays described herein.

公开(公告)号：US12152980B2

公开(公告)日：2024-11-26

申请号：US18633224

申请日：2024-04-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC: G01N15/14 ,  B01L3/00 ,  C12M1/00 ,  G01N1/20 ,  G01N1/28 ,  G01N1/40 ,  G01N15/1433 ,  G01N35/00 ,  G01N35/10 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69 ,  B01L3/02 ,  G01N15/01 ,  G01N15/10 ,  G01N15/149

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US20240384335A1

公开(公告)日：2024-11-21

申请号：US18666949

申请日：2024-05-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas Houser ,  Andrew M. Prantner

IPC: C12Q1/6851 ,  C12Q1/6806 ,  C12Q1/686

Abstract: The invention relates to methods for identifying, determining and quantifying linkages in fragments of nucleic acids.

公开(公告)号：US12148511B2

公开(公告)日：2024-11-19

申请号：US17338937

申请日：2021-06-04

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John Yundt-Pacheco ,  Curtis Parvin

IPC: G16H10/40

Abstract: A clinical diagnostic analyzer for performing a virtual crossover study on quality control (QC) material includes a processor, memory, measurement hardware, and an input panel/display. The analyzer acquires data from a peer group relating to a new lot of quality control material to calculate a predicted mean and standard deviation based on that peer group data and adjusted for bias in the laboratory process. As new analyses are run on the new lot of QC material, the predicted mean and standard deviation are updated to incorporate the actual data on a weighted basis.