公开(公告)号：US20170166931A1

公开(公告)日：2017-06-15

申请号：US15323360

申请日：2015-07-02

Applicant: Genomatica, Inc.

Inventor： Stephanie J. Culler ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P7/04 ,  C12P5/02 ,  C12N15/52

Abstract: The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.

公开(公告)号：US20170159075A1

公开(公告)日：2017-06-08

申请号：US15039221

申请日：2014-11-25

Applicant: GENOMATICA, INC.

Inventor： Robin E. OSTERHOUT ,  Anthony P. BURGARD ,  Priti PHARKYA ,  Stefan ANDRAE

IPC: C12P5/02 ,  C12P7/04 ,  C12N15/81 ,  C12P17/10 ,  C12P13/00 ,  C12N15/70 ,  C12P7/18 ,  C12P7/44

Abstract: The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention.

公开(公告)号：US09657316B2

公开(公告)日：2017-05-23

申请号：US13975678

申请日：2013-08-26

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/24 ,  C12P7/28 ,  C12P7/18 ,  C12N1/20 ,  C12N1/00 ,  C07H21/04 ,  C08G18/00 ,  C08G63/00 ,  C08G63/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C07D207/12 ,  C08G18/32 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/88 ,  C12P19/02

CPC classification number: C12P7/18 ,  C07D207/12 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02 ,  C12Y101/01224 ,  C12Y101/01244 ,  C12Y101/02007 ,  C12Y105/0102 ,  C12Y201/01 ,  C12Y201/0109 ,  C12Y402/01 ,  Y02P20/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

公开(公告)号：US20170073708A1

公开(公告)日：2017-03-16

申请号：US15140384

申请日：2016-04-27

Applicant: Genomatica, Inc.

Inventor： Liang WU ,  Axel Christoph TREFZER ,  Stefaan Marie Andre DE WILDEMAN ,  Marco Alexander VAN DEN BERG

IPC: C12P7/44 ,  C12P7/62 ,  C12N15/70 ,  C12P17/10

CPC classification number: C12P7/44 ,  C12N15/70 ,  C12P7/62 ,  C12P17/10

Abstract: The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid (6-ACA), a method for preparing 5-formyl valeric acid (5-FVA), and a method for preparing caprolactam. Further, the invention relates to a host cell for use in a method according to the invention.

Abstract translation: 本发明涉及一种制备己二酸酯或硫酯的方法。 本发明还涉及从所述酯或硫酯制备己二酸的方法。 此外，本发明提供了许多用于制备所述酯或硫酯的中间体的方法。 本发明还涉及制备6-氨基己酸（6-ACA）的方法，5-甲酰戊酸（5-FVA）的制备方法和己内酰胺的制备方法。 此外，本发明涉及用于根据本发明的方法的宿主细胞。

公开(公告)号：US09556461B2

公开(公告)日：2017-01-31

申请号：US14226631

申请日：2014-03-26

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Mark J. Burk

IPC: C12N1/00 ,  C12N1/26 ,  C12N9/10 ,  C12P7/00 ,  C12P7/62 ,  C12N15/52 ,  C12P5/00 ,  C12P5/02 ,  C12P7/16 ,  C12P7/40 ,  C12P9/00 ,  A61K38/00

CPC classification number: C12P7/62 ,  C12N15/52 ,  C12P5/005 ,  C12P5/026 ,  C12P7/16 ,  C12P7/40 ,  C12P9/00 ,  Y02E50/10

Abstract: The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.

Abstract translation: 本发明提供了具有甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯 ，2,4-戊二烯酸酯，3-丁烯-1醇或1,3-丁二烯途径。 本发明另外提供了使用这些生物体来生产甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯 ，2,4-戊二烯酸酯，3-丁烯-1醇或1,3-丁二烯。

公开(公告)号：US20170008828A1

公开(公告)日：2017-01-12

申请号：US15182245

申请日：2016-06-14

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Priti Pharkya ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Christophe H. Schilling

IPC: C07C51/353 ,  C12P7/46 ,  C12P7/62 ,  C07C67/08

CPC classification number: C07C51/353 ,  C07C51/38 ,  C07C57/04 ,  C07C67/08 ,  C07C67/32 ,  C07C67/333 ,  C12P7/40 ,  C12P7/46 ,  C12P7/62 ,  Y02P20/125 ,  C07C69/60 ,  C07C69/54

Abstract: The invention provides a method of producing acrylic acid. The method includes contacting fumaric acid with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylic acid per mole of fumaric acid. Also provided is an acrylate ester. The method includes contacting fumarate diester with a sufficient amount of ethylene in the presence of a cross-metathesis transformation catalyst to produce about two moles of acrylate ester per mole of fumarate diester. An integrated process for process for producing acrylic acid or acrylate ester is provided which couples bioproduction of fumaric acid with metathesis transformation. An acrylic acid and an acrylate ester production also is provided.

Abstract translation: 本发明提供了制备丙烯酸的方法。 该方法包括在交叉复分解转化催化剂存在下使富马酸与足够量的乙烯接触，以产生约2摩尔丙烯酸/摩尔富马酸。 还提供了丙烯酸酯。 该方法包括在交叉复分解转化催化剂存在下使富马酸二酯与足够量的乙烯接触，以产生每摩尔富马酸二酯约2摩尔丙烯酸酯。 提供了用于生产丙烯酸或丙烯酸酯的方法的综合方法，其将富马酸的生物生产与易位转化相结合。 还提供丙烯酸和丙烯酸酯生产。

公开(公告)号：US20160355855A1

公开(公告)日：2016-12-08

申请号：US14995069

申请日：2016-01-13

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Priti PHARKYA ,  Mark J. BURK

IPC: C12P17/08 ,  C08G63/08 ,  C12N15/52 ,  C12N5/00 ,  C07D313/04

CPC classification number: C12P17/08 ,  C07D313/04 ,  C08G18/4277 ,  C08G63/08 ,  C12N5/0018 ,  C12N15/52 ,  C12N15/63 ,  C12N15/70 ,  C12P17/02

Abstract: The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone.

公开(公告)号：US09458480B2

公开(公告)日：2016-10-04

申请号：US13730612

申请日：2012-12-28

Applicant: GENOMATICA, INC.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C08G69/10 ,  C12P17/12 ,  C12P13/00 ,  C12N1/00 ,  C12N1/20 ,  C08G69/26 ,  C08G73/00 ,  C07C205/00 ,  C07C211/00 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/02 ,  C12P17/10

CPC classification number: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/12

Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：US20160230195A1

公开(公告)日：2016-08-11

申请号：US14935182

申请日：2015-11-06

Applicant: Genomatica, Inc.

Inventor： Robin E. OSTERHOUT ,  Anthony P. BURGARD ,  Mark J. BURK

IPC: C12P7/16 ,  C12N15/70

CPC classification number: C12P7/16 ,  C12N15/70 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/40 ,  Y02E50/10

Abstract: The invention provides non-naturally occurring microbial organisms containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways comprising at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. The invention additionally provides methods of using such microbial organisms to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol, by culturing a non-naturally occurring microbial organism containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways as described herein under conditions and for a sufficient period of time to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol.

公开(公告)号：US09382556B2

公开(公告)日：2016-07-05

申请号：US14450150

申请日：2014-08-01

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

IPC: C12P7/46 ,  C12P7/44 ,  C12P13/00 ,  C12P13/02 ,  C12P17/10 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/80 ,  C12N9/88

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.