公开(公告)号：US10106843B2

公开(公告)日：2018-10-23

申请号：US14934946

申请日：2015-11-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Colin J. H. Brenan ,  Thomas B. Morrison ,  Tanya S. Kanigan

IPC: B01L7/00 ,  C40B60/00 ,  C12Q1/686 ,  B01L3/00

Abstract: One aspect of the invention provides container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a conductive member for heating the interior volume. Another aspect of the invention provides container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a plurality of conductive members for heating an interior volume. Another aspect of the invention provides a container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a first conductive member located in the interior volume and adapted to contact a first end of the microfluidic array.

公开(公告)号：US20180298453A1

公开(公告)日：2018-10-18

申请号：US15947750

申请日：2018-04-06

Applicant: Life Technologies Corporation

Inventor： Rixun FANG ,  Manohar FURTADO ,  Manfred KAYSER ,  Kaye BALLANTYNE

IPC: C12Q1/6888

Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.

公开(公告)号：US10100248B2

公开(公告)日：2018-10-16

申请号：US14865264

申请日：2015-09-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joseph Bartel

IPC: C09K11/56 ,  C09K11/08 ,  C09K11/02 ,  C09K11/88

Abstract: Semiconductor nanocrystal compositions comprising magnesium containing shells and methods of preparing them are described. The compositions provide strong emission in the blue and green wavelengths as well as chemical and photostability that have not been achieved with conventional shell materials.

公开(公告)号：US10093974B2

公开(公告)日：2018-10-09

申请号：US15621341

申请日：2017-06-13

Applicant: Life Technologies Corporation

Inventor： Joseph Beechem ,  Theo Nikiforov ,  Vi-En Choong ,  Xinzhan Peng ,  Guobin Luo ,  Cheng-Yao Chen ,  Michael Previte

IPC: C12N9/12 ,  C12Q1/6869 ,  C07H19/20 ,  G01N21/64 ,  G01N33/58 ,  C12N9/96 ,  C12Q1/6818

Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

公开(公告)号：US10093973B2

公开(公告)日：2018-10-09

申请号：US15169854

申请日：2016-06-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen P. Hendricks ,  Michael Phelan ,  Marian Peris ,  Cheng-Yao Chen ,  Daniel Mazur ,  Xinzhan Peng ,  Amy Castillo

IPC: C12P19/34 ,  C12Q1/6869 ,  C07H19/20 ,  C12N9/12 ,  C12N9/96 ,  C12Q1/6818 ,  G01N33/58 ,  G01N21/64

Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

公开(公告)号：US20180284009A1

公开(公告)日：2018-10-04

申请号：US15941791

申请日：2018-03-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gregory KADUCHAK ,  Michael WARD

IPC: G01N15/14 ,  G01N21/53 ,  G01N15/02 ,  G01N21/64 ,  G01J3/44

CPC classification number: G01N15/1436 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2400/082 ,  G01J3/4406 ,  G01N15/0227 ,  G01N15/0272 ,  G01N15/1404 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1456 ,  G01N15/1459 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/532 ,  G01N21/6456 ,  G01N2015/0003 ,  G01N2015/0011 ,  G01N2015/03 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2015/1422 ,  G01N2015/1438 ,  G01N2015/1445 ,  G01N2015/1452 ,  G01N2015/149

Abstract: The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

公开(公告)号：US10087280B2

公开(公告)日：2018-10-02

申请号：US15491650

申请日：2015-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Xin Wang ,  Hee Chol Kang ,  Aimei Chen ,  Yexin Wu ,  Yi-Zhen Hu ,  Robert Aggeler ,  Quentin Low

IPC: C08G61/12 ,  C07F7/08 ,  C08L65/00 ,  G01N33/533 ,  G01N33/58 ,  H01L51/00 ,  C09K11/06 ,  G01N33/545

Abstract: Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.

公开(公告)号：US10084042B2

公开(公告)日：2018-09-25

申请号：US15867684

申请日：2018-01-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eric Welch ,  Joseph Bartel ,  Eric Tulsky ,  Joseph Treadway ,  Yongfen Chen

IPC: H01L29/06 ,  H01L33/04 ,  H01L31/0352 ,  G01N33/58 ,  H01L21/02 ,  H01L51/00 ,  G01N33/533 ,  B82Y30/00 ,  C30B29/60 ,  C30B29/48 ,  C30B29/40 ,  C30B7/00 ,  B82Y20/00 ,  B82Y40/00

CPC classification number: H01L29/0665 ,  B82Y20/00 ,  B82Y30/00 ,  B82Y40/00 ,  C30B7/00 ,  C30B29/40 ,  C30B29/48 ,  C30B29/60 ,  G01N33/533 ,  G01N33/588 ,  H01L21/02628 ,  H01L31/035209 ,  H01L33/04 ,  H01L51/0007

Abstract: A population of bright and stable nanocrystals is provided. The nanocrystals include a semiconductor core and a thick semiconductor shell and can exhibit high extinction coefficients, high quantum yields, and limited or no detectable blinking.

公开(公告)号：US10082518B2

公开(公告)日：2018-09-25

申请号：US15353828

申请日：2016-11-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Glen Davis ,  John Jackson ,  Gregory Kaduchak ,  Wesley Smith ,  Michael Ward

IPC: G01N15/14 ,  G01N35/10 ,  G01N35/00

CPC classification number: G01N35/1011 ,  G01N15/1404 ,  G01N35/0099

Abstract: A sampling system is provided. The sampling system includes a housing. Mounted to the housing is a Hall effect sensor. A probe configured to contact a sample is inserted into the housing. The probe includes an elongated portion and a restorative spring inserted onto the elongated portion of the probe. The restorative spring provides sufficient restorative force to return the probe to a relaxed position. The Hall effect sensor is configured to sense a field strength generated by the proximity of the restorative spring of the probe in the extended position.

公开(公告)号：US10081833B2

公开(公告)日：2018-09-25

申请号：US14856448

申请日：2015-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu Chen ,  Ruoying Tan

IPC: C12Q1/68 ,  C12Q1/6858 ,  C12Q1/6883 ,  C12Q1/686

CPC classification number: C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2561/113 ,  C12Q2561/101 ,  C12Q2537/161 ,  C12Q2525/186 ,  C12Q2565/107 ,  C12Q2535/125

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).