公开(公告)号：US09523117B2

公开(公告)日：2016-12-20

申请号：US14002662

申请日：2012-01-30

申请人： Hyun Gyu Park ,  Sung Chul Shin ,  Gahee Kim ,  Byoung-Cheorl Kang

发明人： Hyun Gyu Park ,  Sung Chul Shin ,  Gahee Kim ,  Byoung-Cheorl Kang

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6853 ,  C12Q1/6858 ,  C12Q2525/155 ,  C12Q2531/113 ,  C12Q2561/125 ,  C12Q2563/131 ,  C12Q2563/167

摘要： The present invention relates to a method for analyzing genes using SDL-PCR (separation of displaced ligation probe-based PCR), and more particularly to a method for analyzing genes using SDL-PCR, in which probes comprising a nucleotide sequence complementary to the gene of interest are ligated with each other by ligase, and another probe capable of hybridizing to the probes is hybridized and extended, thereby preparing a template probe, and the template probe for the gene of interest is amplified using universal primers.According to the SDL-PCR method of the present invention, non-specific amplification can be minimized by removing non-ligated probes or genomic DNA using a tag, and separation can be achieved within a shorter time compared to a separation method that is performed using exonuclease. In addition, ligation, separation and polymerase chain reaction processes can be performed in a single solution in a single tube, and thus a plurality of genes can be amplified at the same time in an accurate and rapid manner.

摘要翻译： 根据本发明的SDL-PCR方法，通过使用标签除去未连接的探针或基因组DNA，可以使非特异性扩增最小化，并且与使用 核酸外切酶 此外，连接，分离和聚合酶链反应方法可以在单个管中的单个溶液中进行，因此可以以准确和快速的方式同时扩增多个基因。

公开(公告)号：US09434998B2

公开(公告)日：2016-09-06

申请号：US14361678

申请日：2011-11-29

申请人： Hyun Gyu Park ,  Ye Lim Jung ,  Won-Young Chung ,  Ki Soo Park ,  Cheulhee Jung ,  Sung Chul Shin ,  Dae-Yeon Cho ,  Sangjoon Hwang ,  Hyo Won Park

发明人： Hyun Gyu Park ,  Ye Lim Jung ,  Won-Young Chung ,  Ki Soo Park ,  Cheulhee Jung ,  Sung Chul Shin ,  Dae-Yeon Cho ,  Sangjoon Hwang ,  Hyo Won Park

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/70

CPC分类号： C12Q1/689 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q1/6895 ,  C12Q1/705 ,  C12Q2600/112 ,  C12Q2600/16

摘要： A DNA chip for diagnosis of genitourinary infection, and in particular, a DNA chip for diagnosis of genitourinary infection on which oligonucleotide probes for detecting fourteen genitourinary infection pathogens are immobilized is provided. The DNA chip for diagnosis of genitourinary infections may accurately and rapidly analyze infections of fourteen genitourinary infections including multiple infections in various samples with high sensitivity, high specificity, and high reproducibility. Accordingly, the DNA chip may be used for diagnosis and treatment of genitourinary infections in primary health care institutions.

摘要翻译： 提供用于诊断泌尿生殖感染的DNA芯片，特别是用于诊断泌尿生殖感染的DNA芯片，其中用于检测14种泌尿生殖感染病原体的寡核苷酸探针是固定的。 用于诊断泌尿生殖感染的DNA芯片可以准确和快速地分析14种生殖泌尿感染，包括多种感染，多种感染，高灵敏度，高特异性和高重现性的感染。 因此，DNA芯片可用于诊断和治疗初级卫生保健机构的泌尿生殖感染。

公开(公告)号：US08992328B2

公开(公告)日：2015-03-31

申请号：US13698482

申请日：2011-05-17

申请人： Jung Han Kim ,  Hyun Chul Kim ,  Hyun Gyu Park

发明人： Jung Han Kim ,  Hyun Chul Kim ,  Hyun Gyu Park

IPC分类号： A63F13/00 ,  A63F13/30 ,  H04L29/06

CPC分类号： A63F13/352 ,  A63F13/12 ,  A63F13/335 ,  A63F13/49 ,  A63F13/71 ,  A63F2300/513 ,  A63F2300/531 ,  A63F2300/554 ,  A63F2300/807 ,  H04L67/38

摘要： Disclosed are a method, an apparatus, and a recording medium for playing a game with server transferring in an online game environment. The method includes: receiving identification information for login from a first client; transmitting online game environment information to the first client; performing an advance operation of the server transferring for a server transferring of a second client with an outside server; receiving data for playing the game transmitted from the outside server or the second client; transmitting a resulting data of game play, in which the received data is applied to the online game environment, to the outside server or the second client, which has transmitted the data for playing the game; and storing a result of an interaction generated between the first client and the second client in a game history of the first client.

摘要翻译： 公开了一种用于在在线游戏环境中玩服务器传送的游戏的方法，装置和记录介质。 该方法包括：从第一客户端接收用于登录的识别信息; 将网络游戏环境信息传送给第一客户端; 执行所述服务器的传送的前进操作，用于服务器与外部服务器传送第二客户端; 从外部服务器或第二客户端接收用于播放游戏的数据; 将接收到的数据应用于在线游戏环境的游戏结果数据发送到已经发送用于玩游戏的数据的外部服务器或第二客户端; 以及将在所述第一客户端和所述第二客户端之间生成的交互的结果存储在所述第一客户端的游戏历史中。

公开(公告)号：US20140231122A1

公开(公告)日：2014-08-21

申请号：US14119122

申请日：2012-05-21

申请人： Jae Man Park ,  Eun Jin Kim ,  Hae Yeon Kim ,  Hyun Gyu Park ,  Yun Ho An ,  In Hee Cho

发明人： Jae Man Park ,  Eun Jin Kim ,  Hae Yeon Kim ,  Hyun Gyu Park ,  Yun Ho An ,  In Hee Cho

IPC分类号： H05K1/05 ,  C09K5/14

CPC分类号： H05K1/05 ,  C08G59/621 ,  C08G59/686 ,  C08K3/013 ,  C08K3/36 ,  C08K2003/2227 ,  C08K2003/282 ,  C08K2003/385 ,  C08L63/00 ,  C09K5/14 ,  H05K1/056 ,  H05K2201/0209 ,  H05K2201/10106

摘要： An epoxy resin composition having an epoxy resin, a curing agent, and inorganic filler as main components is provided. The epoxy resin includes an epoxy resin of Chemical Formula. Accordingly, the thermal conductivity of the epoxy resin composition can be increased because the epoxy resin has a mesogen structure that facilitates crystallizability. In addition, a high radiant heat board can be provided by using the above-mentioned epoxy resin as an insulating material for a printed circuit board.

摘要翻译： 提供具有环氧树脂，固化剂和无机填料作为主要成分的环氧树脂组合物。 环氧树脂包括化学式的环氧树脂。 因此，环氧树脂组合物的导热性可以提高，因为环氧树脂具有促进结晶性的介晶结构。 此外，通过使用上述环氧树脂作为印刷电路板的绝缘材料，可以提供高辐射热板。

公开(公告)号：US20140171334A1

公开(公告)日：2014-06-19

申请号：US14002662

申请日：2012-01-30

申请人： Hyun Gyu Park ,  Sung Chul Shin ,  Gahee Kim ,  Vyoung-Cheorl Kang

发明人： Hyun Gyu Park ,  Sung Chul Shin ,  Gahee Kim ,  Vyoung-Cheorl Kang

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6853 ,  C12Q1/6858 ,  C12Q2525/155 ,  C12Q2531/113 ,  C12Q2561/125 ,  C12Q2563/131 ,  C12Q2563/167

摘要： The present invention relates to a method for analyzing genes using SDL-PCR (separation of displaced ligation probe-based PCR), and more particularly to a method for analyzing genes using SDL-PCR, in which probes comprising a nucleotide sequence complementary to the gene of interest are ligated with each other by ligase, and another probe capable of hybridizing to the probes is hybridized and extended, thereby preparing a template probe, and the template probe for the gene of interest is amplified using universal primers.According to the SDL-PCR method of the present invention, non-specific amplification can be minimized by removing non-ligated probes or genomic DNA using a tag, and separation can be achieved within a shorter time compared to a separation method that is performed using exonuclease. In addition, ligation, separation and polymerase chain reaction processes can be performed in a single solution in a single tube, and thus a plurality of genes can be amplified at the same time in an accurate and rapid manner.

摘要翻译： 本发明涉及使用SDL-PCR（基于取代的基于连接探针的PCR的分离）分析基因的方法，更具体地涉及使用SDL-PCR分析基因的方法，其中探针包含与该基因互补的核苷酸序列 通过连接酶连接相互连接，并且能够与探针杂交的另一个探针杂交并扩展，从而制备模板探针，并使用通用引物扩增目的基因的模板探针。 根据本发明的SDL-PCR方法，通过使用标签除去未连接的探针或基因组DNA，可以使非特异性扩增最小化，并且与使用 核酸外切酶 此外，连接，分离和聚合酶链反应方法可以在单个管中的单个溶液中进行，因此可以以准确和快速的方式同时扩增多个基因。

公开(公告)号：US08105977B2

公开(公告)日：2012-01-31

申请号：US12189074

申请日：2008-08-08

申请人： Kuk-Hwan Kim ,  Yang-Kyu Choi ,  Oktay Yarimaga ,  Hyun Gyu Park ,  Tae Won Kim ,  Yun Kyung Jung

发明人： Kuk-Hwan Kim ,  Yang-Kyu Choi ,  Oktay Yarimaga ,  Hyun Gyu Park ,  Tae Won Kim ,  Yun Kyung Jung

IPC分类号： B41M5/26

CPC分类号： D21H21/44 ,  B42D15/0033 ,  B42D25/29 ,  Y10T428/24934

摘要： The present invention relates to a counterfeit prevention paper and manufacturing method thereof, and more particularly to currency, securities, official document and several certificates, etc.The counterfeit prevention paper according to the present invention comprises a paper and a thermopaint layer which is formed on a paper and is discolored according to a temperature.The counterfeit prevention paper according to the present invention can detect easily a counterfeit by an unaided eye. Also, a function for preventing a counterfeit is not copied by a counterfeit device, thus the counterfeit prevent paper according to the present invention can improve a reliability of various official documents and several certificates, etc.

摘要翻译： 本发明涉及防伪纸及其制造方法，更具体地涉及货币，证券，官方文件和若干证书等。根据本发明的防伪纸包括纸和热电影层，其形成在 一张纸，根据温度变色。 根据本发明的防伪纸可以通过肉眼容易地检测伪造的伪造品。 此外，防伪品的功能不被仿冒装置复制，因此根据本发明的防伪纸可以提高各种官方文件和几种证书等的可靠性。

公开(公告)号：US20100232040A1

公开(公告)日：2010-09-16

申请号：US12651520

申请日：2010-01-04

申请人： Yoshito Iwasawa ,  Hyun-gyu Park

发明人： Yoshito Iwasawa ,  Hyun-gyu Park

IPC分类号： G02B9/12 ,  G02B9/10

CPC分类号： G02B27/0068 ,  G02B7/021 ,  G03B3/02 ,  G03B2217/002

摘要： A photographic lens of a photographic apparatus which includes a plurality of lens groups may be manufactured using a method which includes correcting a focus deviation from a designed value generated in one lens group by adjusting a distance between lenses of another lens group. The distance between lenses may be adjusted using a washer. The lenses between which the distance is adjusted may include a positive lens and a negative lens, and the lens group having the lenses between which the distance is adjusted may have a negative refractive power.

摘要翻译： 可以使用包括通过调整另一透镜组的透镜之间的距离来校正与一个透镜组中产生的设计值的聚焦偏差的方法来制造包括多个透镜组的摄影装置的摄影镜头。 透镜之间的距离可以使用洗衣机进行调整。 调整距离的透镜可以包括正透镜和负透镜，并且具有调整距离的透镜之间的透镜组可具有负折光力。

公开(公告)号：US20060188906A1

公开(公告)日：2006-08-24

申请号：US11335939

申请日：2006-01-20

申请人： Joon-ho Kim ,  Jun-hong Min ,  Kyung-hwan Park ,  Soo-suk Lee ,  Hyun-gyu Park

发明人： Joon-ho Kim ,  Jun-hong Min ,  Kyung-hwan Park ,  Soo-suk Lee ,  Hyun-gyu Park

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B01L3/502707 ,  B01J2219/00522 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00632 ,  B01J2219/00644 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/0072 ,  B01L3/5025 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0887 ,  B82Y30/00

摘要： Provided are a microfluidic chip for multiple bioassay and a method of manufacturing the same. The method includes: forming microfluidic channels by coupling a channel structure having grooves for sample channels and probe channels in a bottom surface and a substrate; forming probe immobilization regions at intersections between the probe channels and the sample channels; and forming blocking walls in the probe channels before and after each of the probe immobilization regions to prevent mixing of target sample. Since the probes are immobilized in the microfluidic channels after the formation of the microfluidic channels, difficulty in connecting fluidic channels after the immobilization of probes on a substrate can be removed. In addition, a microfluidic platform for a multiple bioassay into which a plurality of samples can be simultaneously loaded can be formed.

摘要翻译： 提供了用于多重生物测定的微流控芯片及其制造方法。 该方法包括：通过在底表面和基底中耦合具有用于样品通道和探针通道的沟槽的通道结构来形成微流体通道; 在探针通道和样品通道之间的交叉处形成探针固定区域; 并且在每个探针固定区域之前和之后在探针通道中形成阻挡壁以防止靶样品混合。 由于在形成微流体通道之后将探针固定在微流体通道中，因此可以除去在探针固定在基底上之后连接流体通道的困难。 此外，可以形成用于多个生物测定的微流体平台，多个样品可以同时装载到该微生物测定中。