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    Conjugation Of Carboxyl Functional Hydrophilic Beads
    22.
    发明申请
    Conjugation Of Carboxyl Functional Hydrophilic Beads 审中-公开
    羧基官能亲水珠的共轭

    公开(公告)号：US20170002410A1

    公开(公告)日：2017-01-05

    申请号：US15200588

    申请日：2016-07-01

    Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

    Inventor： Geir Fonnum ,  Steven M. Menchen ,  Alfred Lui ,  Guobing Xiang ,  Synne Larsen

    IPC: C12Q1/68

    CPC classification number: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/197 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629

    Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

    Abstract translation: 聚合物基质，例如聚合物涂层或聚合物水凝胶网络，包括羧基部分，其可以用作受体或分析物分子可以连接到的缀合位点。 在一个实例中，聚合物底物包括具有链烷酸部分或其衍生物的聚丙烯酰胺聚合物网络，其可以与羧基活化化合物反应，以在聚丙烯酰胺网络上提供活化的链烷酸酯部分。胺封端的核酸可以与活化的链烷酸酯部分反应 通过烷基酰胺部分将核酸捕获到聚合物网络。

    Automated Superparamagnetic Particle Handling System
    24.
    发明申请
    Automated Superparamagnetic Particle Handling System 审中-公开
    自动超顺磁粒子处理系统

    公开(公告)号：US20160304829A1

    公开(公告)日：2016-10-20

    申请号：US15151587

    申请日：2016-05-11

    Applicant: LIFE TECHNOLOGIES AS

    Inventor： Axl NEURAUTER ,  Lars Norderhaug ,  Ole Brekke ,  Pål Songe

    IPC: C12M1/00 ,  C12N13/00

    CPC classification number: G01N33/82 ,  C12M23/20 ,  C12M47/02 ,  C12N13/00 ,  C40B50/14 ,  G01N33/543 ,  G01N33/54326

    Abstract: Methods of reversal of the binding between a biotin compound and a biotin-binding compound are disclosed. A method of reversibly releasing a biotinylated moiety from a streptavidin (or avidin) coated support is shown as an example. The strong interaction between streptavidin or avidin-biotin is made much weaker by using a combination of modified streptavidin or avidin and modified biotin like desthiobiotin or a derivative thereof like DSB-X Biotin. A protein, such as an antibody may be biotinylated with the modified biotin. When this protein is isolated by binding the modified biotin to the modified streptavidin or avidin bound to an solid surface, it may be released under very gently and very rapid conditions by addition of free biotin. In contrast to proteins obtained by the prior art release methods the protein obtained using the previously available release methods, the proteins obtained using the methods disclosed herein will maintain their native conformation. Uses of the methods in various procedures including cell detachment procedures and techniques of detection, identification, determination, purification, separation and/or isolation of target proteins or nucleic acid molecules are also described.

    Abstract translation: 公开了生物素化合物与生物素结合化合物之间的结合的逆转方法。 示出了从链霉抗生物素蛋白（或抗生物素蛋白）包被的载体中可逆释放生物素化部分的方法。 通过使用经修饰的链霉亲和素或抗生物素蛋白和修饰的生物素如脱硫生物素或其衍生物如DSB-X生物素的组合，链霉抗生物素蛋白或抗生物素蛋白 - 生物素之间的强相互作用变得非常弱。 蛋白质，例如抗体可以用修饰的生物素生物素化。 当通过将修饰的生物素结合到与固体表面结合的经修饰的链霉抗生物素蛋白或抗生物素蛋白来分离该蛋白质时，其可以通过添加游离生物素在非常温和和非常快速的条件下释放。 与通过现有技术释放方法获得的蛋白质相比，使用先前可获得的释放方法获得的蛋白质，使用本文公开的方法获得的蛋白质将保持其天然构象。 还描述了在各种方法中使用方法，包括细胞分离程序以及靶蛋白或核酸分子的检测，鉴定，测定，纯化，分离和/或分离技术。

    METHOD FOR ISOLATING NUCLEIC ACIDS COMPRISING THE USE OF ETHYLENE GLYCOL MULTIMERS
    27.
    发明申请
    METHOD FOR ISOLATING NUCLEIC ACIDS COMPRISING THE USE OF ETHYLENE GLYCOL MULTIMERS 有权
    分离含有乙二醇甘油三酯的核酸的方法

    公开(公告)号：US20140094597A1

    公开(公告)日：2014-04-03

    申请号：US14035890

    申请日：2013-09-24

    Applicant: LIFE TECHNOLOGIES AS

    Inventor： Erling FINNE

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12N15/1013

    Abstract: The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a solid support, preferably magnetic beads, in the presence of an ethylene glycol multimer consisting of from 2 to 70 ethylene oxide monomers, preferably tetraethylene glycol, whereby soluble nucleic acid in said sample is bound to the surface of the support, and separating said support with bound nucleic acid from the sample. Kits for performance of the invention are also provided.

    Abstract translation: 本发明提供了从样品中分离核酸的方法，所述方法包括在由2-70个环氧乙烷单体组成的乙二醇多聚体存在下使所述样品与固体支持物，优选磁珠接触，优选四甘醇 由此将所述样品中的可溶性核酸结合到载体的表面，并用结合的核酸从样品中分离所述载体。 还提供了用于实现本发明的套件。

    Methods and compositions for coupling nucleic acid to a functionalized support
    28.
    发明授权
    Methods and compositions for coupling nucleic acid to a functionalized support 有权

    公开(公告)号：US12152235B2

    公开(公告)日：2024-11-26

    申请号：US18069171

    申请日：2022-12-20

    Applicant: LIFE TECHNOLOGIES AS

    Inventor： Laure Jobert

    IPC: C12N15/10

    Abstract: This invention relates to methods and compositions for coupling nucleic acid to a functionalized surface or support. In particular, the present invention provides an improved process for coupling aminated nucleic acid to a support functionalized with carboxylic acid groups, wherein the coupling reaction is conducted in the presence of an organic solvent. The invention further relates to compositions and kits for performing the coupling reaction and uses of nucleic acid-loaded supports for various applications.

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