公开(公告)号：US06977305B2

公开(公告)日：2005-12-20

申请号：US09968401

申请日：2001-09-29

Applicant: Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

Inventor： Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

IPC: C07C66/02 ,  C07D209/08 ,  C07D209/12 ,  C07D209/14 ,  C07D209/16 ,  C07D209/24 ,  C07D209/54 ,  C07D209/60 ,  C07D403/14 ,  C07D417/06 ,  C07D471/04 ,  C09B23/02 ,  G01N33/58 ,  C07D209/56 ,  C07D209/02

CPC classification number: G01N1/30 ,  A61K41/0057 ,  C07C66/02 ,  C07D209/08 ,  C07D209/12 ,  C07D209/14 ,  C07D209/16 ,  C07D209/18 ,  C07D209/24 ,  C07D209/54 ,  C07D209/60 ,  C07D209/96 ,  C07D401/04 ,  C07D403/06 ,  C07D403/12 ,  C07D403/14 ,  C07D417/06 ,  C07D417/14 ,  C07D471/04 ,  C07D498/04 ,  C07K1/13 ,  C09B23/02 ,  C09B23/06 ,  C09B23/083 ,  C09B23/107 ,  C12Q1/6825 ,  C40B70/00 ,  G01N31/22 ,  G01N33/533 ,  G01N33/535 ,  G01N33/58 ,  G01N33/581 ,  G01N33/582 ,  G01N2001/302 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

公开(公告)号：US20050208534A1

公开(公告)日：2005-09-22

申请号：US11005861

申请日：2004-12-06

Applicant: Jason Dallwig ,  David Hagen ,  Ching-Ying Cheung ,  Gerald Thomas ,  Stephen Yue

Inventor： Jason Dallwig ,  David Hagen ,  Ching-Ying Cheung ,  Gerald Thomas ,  Stephen Yue

IPC: C07D413/02 ,  C07D417/02 ,  C07H21/04 ,  C12Q1/68

CPC classification number: C09B23/164 ,  C07D277/64 ,  C07D417/06 ,  C09B23/0008 ,  C09B23/04 ,  C09B23/06 ,  C09B23/107 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/16 ,  G01N33/5308 ,  Y10T436/143333

Abstract: Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.

Abstract translation: 具有作为核酸污渍的取代的次甲基部分的花青染料化合物，特别是用于RNA的荧光染色，包括具有下式的化合物：其中R 1是C 1 -C 3烷基， 烷基，磺基烷基，羧基烷基或C 1 -C 6烷氧基; 每个R 2独立地选自H，C 1 -C 6烷基，C 1至C 6烷基， -C 6烷氧基，稠合的苯并，三氟甲基，氨基，磺基，羧基和卤素，其任选进一步被取代; R 3，R 4和R 5中的至少一个是任选地是烷基，芳基，杂芳基，环状或杂环部分 被烷基，氨基，氨基烷基，羧基，硝基或卤素取代; 而其余的R 3，R 4或R 5是氢; X是S，O或Se; 并且D是取代或未取代的吡啶鎓，喹啉鎓或苯并咪唑鎓部分。

公开(公告)号：US06579718B1

公开(公告)日：2003-06-17

申请号：US09632927

申请日：2000-08-04

Applicant: Stephen T. Yue ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Ching-Ying Cheung ,  Richard P. Haugland

Inventor： Stephen T. Yue ,  Thomas H. Steinberg ,  Wayne F. Patton ,  Ching-Ying Cheung ,  Richard P. Haugland

IPC: G01N3348

CPC classification number: C07D401/06 ,  C07D209/08 ,  C07D209/86 ,  C07D213/20 ,  C07D215/10 ,  C07D277/64 ,  C07D417/06 ,  C09B23/105 ,  G01N33/6809 ,  Y10T436/25125 ,  Y10T436/25375

Abstract: The present invention describes a variety of substituted and unsubstituted carbazolylvinyl dyes and their use for detecting and quantifying poly(amino acids), including peptides, polypeptides and proteins. The labeled proteins or peptides are highly colored, but are also detected by their strong fluorescence enhancement. Poly(amino acids) are detected in solution, in electrophoretic gels, and on solid supports, including blots and dipsticks. The present method of staining is highly sensitive, extremely facile, and relatively non-selective and can be accomplished without the use of organic solvent additives.

Abstract translation: 本发明描述了各种取代和未取代的咔唑基乙烯基染料及其用于检测和定量聚（氨基酸）的用途，包括肽，多肽和蛋白质。 标记的蛋白质或肽是高度着色的，但也通过其强荧光增强来检测。 聚（氨基酸）在溶液中，电泳凝胶和固体支持物上检测，包括印迹和浸渍。 本发明的染色方法是高度敏感的，非常容易的和相对非选择性的，并且可以在不使用有机溶剂添加剂的情况下完成。

公开(公告)号：US08819252B1

公开(公告)日：2014-08-26

申请号：US10139095

申请日：2002-05-03

Applicant: Ronald W. Szeto ,  David Chun Ying Cheung ,  Rajkumar Jalan ,  Sridhar J. Devarapalli

Inventor： Ronald W. Szeto ,  David Chun Ying Cheung ,  Rajkumar Jalan ,  Sridhar J. Devarapalli

IPC: G06F15/16

CPC classification number: H04L63/1458 ,  H04L63/108 ,  H04L67/1008

Abstract: Transaction rate limiting is provided to monitor new connections. If the number of new connections requested by a particular client exceeds a predetermined threshold value, then the client may be frozen out for a configured period of time. By denying access for the configured period of time, the client is prevented from monopolizing a particular client. Additionally, if the client does have malicious intent, a denial of service attack may be thwarted. The denial of service may be accomplished without alerting the client. This prevents a malicious client from regrouping and attempting an assault via a different mechanism.

Abstract translation: 提供事务速率限制来监视新的连接。 如果特定客户端请求的新连接的数量超过预定阈值，则客户端可以在已配置的时间段内被冻结。 通过在配置的时间段内拒绝访问，客户端被阻止垄断特定客户端。 另外，如果客户端确实有恶意的意图，则拒绝服务攻击可能会被阻止。 拒绝服务可以在不告知客户的情况下完成。 这样可以防止恶意客户端通过不同的机制重新组合和尝试攻击。

公开(公告)号：US20120329068A1

公开(公告)日：2012-12-27

申请号：US13423092

申请日：2012-03-16

Applicant: Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Hye Eun Hoover

Inventor： Fei Mao ,  Wai-Yee Leung ,  Ching-Ying Cheung ,  Hye Eun Hoover

IPC: G01N21/64 ,  G01N33/53 ,  C12N9/96 ,  C07K1/113

CPC classification number: G01N1/30 ,  C07K1/13 ,  C07K17/06 ,  C09B11/24 ,  C09B23/06 ,  C09B23/083 ,  C09B57/001 ,  C09B57/02 ,  G01N33/53 ,  G01N33/532 ,  G01N33/533 ,  G01N33/582 ,  G01N33/6854

Abstract: The present invention provides methods, compositions, and kits useful in preparing labeled molecules, which are useful in the detection of binding partners.

Abstract translation: 本发明提供了可用于制备标记分子的方法，组合物和试剂盒，其可用于检测结合配偶体。

公开(公告)号：US20110171678A1

公开(公告)日：2011-07-14

申请号：US12906304

申请日：2010-10-18

Applicant: Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

Inventor： Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

IPC: C12Q1/02 ,  C07K14/00 ,  C07K2/00 ,  C07D403/14 ,  C07F5/02 ,  C07C245/12 ,  C07F15/00 ,  C08B37/02 ,  C07H21/00 ,  C07D403/06 ,  C07H19/02 ,  C07K14/77 ,  C07D495/04 ,  C12N9/96 ,  C07K16/00 ,  C12N1/00 ,  C07D471/04 ,  G01N1/30

CPC classification number: G01N1/30 ,  A61K41/0057 ,  C07C66/02 ,  C07D209/08 ,  C07D209/12 ,  C07D209/14 ,  C07D209/16 ,  C07D209/18 ,  C07D209/24 ,  C07D209/54 ,  C07D209/60 ,  C07D209/96 ,  C07D401/04 ,  C07D403/06 ,  C07D403/12 ,  C07D403/14 ,  C07D417/06 ,  C07D417/14 ,  C07D471/04 ,  C07D498/04 ,  C07K1/13 ,  C09B23/02 ,  C09B23/06 ,  C09B23/083 ,  C09B23/107 ,  C12Q1/6825 ,  C40B70/00 ,  G01N31/22 ,  G01N33/533 ,  G01N33/535 ,  G01N33/58 ,  G01N33/581 ,  G01N33/582 ,  G01N2001/302 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了结合通过反应性基团或共轭物质在3位被取代的吲哚环部分的化学活性碳菁染料及其用途。 通过该位置的共轭导致光谱性质均匀地优于光谱相似的染料的缀合物，其中附着在不同的位置。 本发明包括具有一个或多个苯并氮的衍生化合物。

公开(公告)号：US20110117591A1

公开(公告)日：2011-05-19

申请号：US12908152

申请日：2010-10-20

Applicant: Ching-Ying CHEUNG ,  Diane R. Gray ,  Stephen T. Yue

Inventor： Ching-Ying CHEUNG ,  Diane R. Gray ,  Stephen T. Yue

IPC: C12Q1/02 ,  C07D417/06 ,  C07D295/116 ,  C07D417/14 ,  C07D211/62 ,  C07D295/145 ,  C07D211/58 ,  C07C225/30 ,  C07C225/28 ,  G01N21/75

CPC classification number: G01N33/1806 ,  C07D211/62 ,  C07D295/112 ,  C07D401/14 ,  C07D417/06 ,  C07D417/14 ,  C09B23/04 ,  C12Q1/04 ,  C12Q1/18 ,  G01N21/643 ,  G01N33/52 ,  G01N33/582 ,  G01N2021/6432

Abstract: Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents.

Abstract translation: 公开了含有荧光团和可还原淬火单元的化学染料化合物。 可还原淬火单元在处于氧化状态时淬灭荧光团。 在还原时，淬火单元的淬火特性减弱或消除。 化合物可用于各种应用，包括检测细菌细胞，监测细菌细胞的电子传递链功能，监测非生物系统的氧化状态，以及测定抗菌剂或抗菌剂的有效性。

公开(公告)号：US20100317543A1

公开(公告)日：2010-12-16

申请号：US12851030

申请日：2010-08-05

Applicant: Jason Alfred DALLWIG ,  David Carl HAGEN ,  Ching-Ying CHEUNG ,  Gerald Alan THOMAS ,  Stephen YUE

Inventor： Jason Alfred DALLWIG ,  David Carl HAGEN ,  Ching-Ying CHEUNG ,  Gerald Alan THOMAS ,  Stephen YUE

IPC: C40B30/04 ,  C07D277/64 ,  C07D417/06 ,  C07D417/14 ,  G01N33/50 ,  C12Q1/68

CPC classification number: C09B23/164 ,  C07D277/64 ,  C07D417/06 ,  C09B23/0008 ,  C09B23/04 ,  C09B23/06 ,  C09B23/107 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/16 ,  G01N33/5308 ,  Y10T436/143333

Abstract: Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.

Abstract translation: 具有作为核酸染色的取代的次甲基部分的花青染料化合物，特别是用于RNA的荧光染色，包括具有下式的化合物，其中R 1是C 1 -C 6烷基，磺基烷基，羧基烷基或C 1 -C 6烷氧基; 每个R 2独立地选自H，C 1 -C 6烷基，C 1 -C 6烷氧基，稠合的苯并，三氟甲基，氨基，磺基，羧基和卤素，其任选进一步被取代; R 3，R 4和R 5中的至少一个是任选被烷基，氨基，氨基烷基，羧基，硝基或卤素取代的烷基，芳基，杂芳基，环状或杂环部分; 剩余的R3，R4或R5是氢; X是S，O或Se; 并且D是取代或未取代的吡啶鎓，喹啉鎓或苯并咪唑鎓部分。

公开(公告)号：US20070102675A1

公开(公告)日：2007-05-10

申请号：US11468736

申请日：2006-08-30

Applicant: Chi-Ming Chan ,  Kai-Mo Ng ,  Catherine Wong ,  Ying Cheung

Inventor： Chi-Ming Chan ,  Kai-Mo Ng ,  Catherine Wong ,  Ying Cheung

IPC: H01B1/12

CPC classification number: H01B1/22 ,  H05K1/0257 ,  H05K1/0259 ,  H05K1/167 ,  H05K2201/0738 ,  Y10T428/257 ,  Y10T428/31663

Abstract: The invention provides a process for preparing an overvoltage protection material comprising: (i) preparing a mixture comprising a polymer binder precursor and a conductive material; and (ii) heating the mixture to cause reaction of the polymer binder precursor and generate a polymer matrix having conductive material dispersed therein, wherein the polymer binder precursor is chosen such that substantially no solvent is generated during the reaction.

Abstract translation: 本发明提供一种制备过电压保护材料的方法，包括：（i）制备包含聚合物粘合剂前体和导电材料的混合物; 和（ii）加热混合物以引起聚合物粘合剂前体的反应并产生其中分散有导电材料的聚合物基质，其中选择聚合物粘合剂前体使得在反应期间基本上不产生溶剂。

公开(公告)号：US06974873B2

公开(公告)日：2005-12-13

申请号：US09969853

申请日：2001-10-01

Applicant: Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

Inventor： Wai-Yee Leung ,  Ching-Ying Cheung ,  Stephen Yue

IPC: C07C66/02 ,  C07D209/08 ,  C07D209/12 ,  C07D209/14 ,  C07D209/16 ,  C07D209/24 ,  C07D209/54 ,  C07D209/60 ,  C07D403/14 ,  C07D417/06 ,  C07D471/04 ,  C09B23/02 ,  G01N33/58 ,  C07D209/02

CPC classification number: G01N1/30 ,  A61K41/0057 ,  C07C66/02 ,  C07D209/08 ,  C07D209/12 ,  C07D209/14 ,  C07D209/16 ,  C07D209/18 ,  C07D209/24 ,  C07D209/54 ,  C07D209/60 ,  C07D209/96 ,  C07D401/04 ,  C07D403/06 ,  C07D403/12 ,  C07D403/14 ,  C07D417/06 ,  C07D417/14 ,  C07D471/04 ,  C07D498/04 ,  C07K1/13 ,  C09B23/02 ,  C09B23/06 ,  C09B23/083 ,  C09B23/107 ,  C12Q1/6825 ,  C40B70/00 ,  G01N31/22 ,  G01N33/533 ,  G01N33/535 ,  G01N33/58 ,  G01N33/581 ,  G01N33/582 ,  G01N2001/302 ,  Y10T436/143333

Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract translation: 描述了结合通过反应性基团或共轭物质在3位被取代的吲哚环部分的化学活性碳菁染料及其用途。 通过该位置的共轭导致光谱性质均匀地优于光谱相似的染料的缀合物，其中附着在不同的位置。 本发明包括具有一个或多个苯并氮的衍生化合物。