公开(公告)号：US09506845B2

公开(公告)日：2016-11-29

申请号：US13781318

申请日：2013-02-28

Applicant: Fluidigm Corporation

Inventor： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C. Norris ,  Dominique G. Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A. A. West

IPC: C12P19/34 ,  G01N1/28 ,  C12Q1/68 ,  G01N1/34 ,  G01N15/14 ,  B01L3/00 ,  B01L7/00

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

公开(公告)号：US20160194685A1

公开(公告)日：2016-07-07

申请号：US14960754

申请日：2016-03-21

Applicant: Fluidigm Corporation

Inventor： Marc A. Unger ,  Geoffrey Richard Facer ,  Barry Clerkson ,  Christopher G. Cesar ,  Neil Switz

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01J2219/00576 ,  B01J2219/00704 ,  B01L3/5027 ,  B01L7/52 ,  G01N21/64 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6486 ,  G01N21/75 ,  G01N21/8483 ,  G01N27/44721 ,  G01N2021/6421 ,  G01N2201/061 ,  G02B21/16 ,  G02B21/36

Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

公开(公告)号：US20140024559A1

公开(公告)日：2014-01-23

申请号：US14031972

申请日：2013-09-19

Applicant: Fluidigm Corporation

Inventor： Marc A. Unger ,  Geoffrey Richard Facer ,  Barry Clerkson ,  Christopher G. Cesar ,  Neil Switz

IPC: G01N21/64

CPC classification number: C12Q1/686 ,  B01J2219/00576 ,  B01J2219/00704 ,  B01L3/5027 ,  B01L7/52 ,  G01N21/64 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6486 ,  G01N21/75 ,  G01N21/8483 ,  G01N27/44721 ,  G01N2021/6421 ,  G01N2201/061 ,  G02B21/16 ,  G02B21/36

Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

Abstract translation: 一种用于从微流体装置成像一个或多个选定的荧光指示的装置。 该装置包括耦合到至少一个微流体装置中的至少一个腔室的成像路径。 成像路径提供从至少一个微流体装置的至少一个室中的一个或多个样品衍生的一个或多个荧光发射信号的传输。 腔室具有腔室尺寸，腔室尺寸的特征在于与成像路径垂直的实际空间尺寸。 该装置还包括耦合到成像路径的光学透镜系统。 光学透镜系统适于透射与腔室相关联的一个或多个荧光信号。

公开(公告)号：US20130302883A1

公开(公告)日：2013-11-14

申请号：US13781292

申请日：2013-02-28

Applicant: FLUIDIGM CORPORATION

Inventor： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C. Norris ,  Dominique G. Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A.A. West

IPC: G01N1/34

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

Abstract translation: 描述了利用微流体的多单细胞捕获和处理的方法，系统和装置。 提供的工具和技术用于捕获，分配和/或操纵来自较大群体的细胞的单个细胞以及产生与每个单个细胞相关的遗传信息和/或反应。 可以使用不同的捕获配置来捕获单个细胞，然后在多室反应配置中处理每个单独的细胞。 一些实施方案可以提供已经从较大群体中划分的多个单个细胞的特异性靶扩增，全基因组扩增，全转录组扩增，实时PCR制备，拷贝数变异，预扩增，mRNA测序和/或单倍型 细胞。 一些实施例可以提供其他应用。 一些实施例可以被配置为用于对作为处理的一部分的各个单元或相关联的反应产物进行成像。 在某些情况下可以收获和/或进一步分析反应产物。

公开(公告)号：US10322411B2

公开(公告)日：2019-06-18

申请号：US15150062

申请日：2016-05-09

Applicant: Fluidigm Corporation

Inventor： Naga Gopi Devaraju ,  Marc A. Unger

IPC: F16K11/20 ,  B01L3/00 ,  F17D3/00 ,  G01N35/00

Abstract: A microfluidic system includes a substrate, a set of input ports coupled to the substrate, and a set of output ports coupled to the substrate. The microfluidic system also includes a microfluidic processing system coupled to the substrate and including a plurality of processing sites. The microfluidic processing system is coupled to the set of input ports and the set of output ports. The microfluidic system further includes one or more microfluidic logic devices coupled to the substrate and operable to control at least a portion of the microfluidic processing system.

公开(公告)号：US20180306683A1

公开(公告)日：2018-10-25

申请号：US15925600

申请日：2018-03-19

Applicant: Fluidigm Corporation

Inventor： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C. Norris ,  Dominique G. Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A.A. West

IPC: G01N1/28 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/14

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

公开(公告)号：US20180243741A1

公开(公告)日：2018-08-30

申请号：US15829665

申请日：2017-12-01

Applicant: Fluidigm Corporation

Inventor： Marc A. Unger ,  Lincoln McBride ,  Geoffrey Facer

IPC: B01L3/00 ,  C12Q1/686 ,  G01N27/453 ,  B01L7/00

CPC classification number: B01L3/502738 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2565/629 ,  G01N27/44791 ,  G01N27/453 ,  C12Q2561/113 ,  C12Q2563/159 ,  C12Q2531/113

Abstract: Devices and methods for performing the relative concentration of a target in a sample, the sample containing both target and non-target components, the method performed by partitioning the sample into a large number of reaction volumes such that the target is concentrated relative to the non-target, and performing a detection assay upon each reaction volume to detect the target.

公开(公告)号：US09952126B2

公开(公告)日：2018-04-24

申请号：US13781313

申请日：2013-02-28

Applicant: Fluidigm Corporation

Inventor： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C Norris ,  Dominique Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A. A. West

IPC: C12P19/34 ,  G01N1/28 ,  C12Q1/68 ,  G01N1/34 ,  G01N15/14 ,  B01L3/00 ,  B01L7/00

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

公开(公告)号：US09593698B2

公开(公告)日：2017-03-14

申请号：US14072243

申请日：2013-11-05

Applicant: Fluidigm Corporation

Inventor： David Fernandes ,  Hou-Pu Chou ,  Marc A. Unger

IPC: F16C3/00 ,  F16K99/00 ,  B01L3/00 ,  F15C3/00 ,  F15C3/04 ,  G06F17/50 ,  H01H29/00

CPC classification number: F15C3/00 ,  A61M2206/22 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2200/14 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655 ,  F15C3/04 ,  F16K99/0001 ,  F16K99/0009 ,  F16K99/0015 ,  F16K99/0034 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0082 ,  F16K2099/0084 ,  G06F17/509 ,  G06F2217/16 ,  H01H2029/008 ,  Y10T137/206 ,  Y10T137/2202 ,  Y10T137/2218 ,  Y10T137/2224 ,  Y10T137/7879 ,  Y10T137/7891 ,  Y10T137/7892

Abstract: A microfabricated fluidic unidirectional valve includes a microfabricated elastomer material having a flow through channel. The microfabricated fluidic unidirectional valve also includes an elastomer flap attached to the elastomer material in the flow through channel. The elastomer flap forms a seal in the flow through channel to prevent fluid from flowing in a first direction through the flow through channel and to allow fluid flow in a second direction through the flow through channel.

Abstract translation: 微制造的流体单向阀包括具有流通通道的微加工弹性体材料。 微制造的流体单向阀还包括在流动通道中附接到弹性体材料的弹性体翼片。 弹性翼片在流通通道中形成密封，以防止流体沿第一方向流过通过通道并允许流体沿第二方向流过通过通道的流体。

公开(公告)号：US20150174575A1

公开(公告)日：2015-06-25

申请号：US14477602

申请日：2015-03-02

Applicant: Fluidigm Corporation

Inventor： Marc A. Unger ,  Lincoln McBride ,  Geoffrey Facer

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/68

CPC classification number: C12Q1/686 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N27/453 ,  C12Q2565/629

Abstract: Devices and methods for performing the relative concentration of a target in a sample, the sample containing both target and non-target components, the method performed by partitioning the sample into a large number of reaction volumes such that the target is concentrated relative to the non-target, and performing a detection assay upon each reaction volume to detect the target.

Abstract translation: 用于执行样品中靶标相对浓度的装置和方法，包含靶标和非目标成分的样品，通过将样品分成大量反应体积进行的方法，使得靶相对于非目标成分集中 并且在每个反应体积上进行检测测定以检测目标。