公开(公告)号：US11021472B2

公开(公告)日：2021-06-01

申请号：US15186580

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: C07D409/04 ,  C12M1/42 ,  C07J51/00 ,  G01N33/543 ,  G01N33/50 ,  C12N5/00

Abstract: The present invention relates to the use compounds comprising two or more hydrophobic domains and a hydrophilic domain comprising a polyethylene glycol (PEG) moiety for stabilization of a cell, and methods related thereto.

公开(公告)号：US20180172680A1

公开(公告)日：2018-06-21

申请号：US15899003

申请日：2018-02-19

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Toralf Zarnt ,  Dieter Heindl ,  Alfons Nichtl ,  Frank Sicherl-Birk

IPC: G01N33/543

Abstract: Disclosed is a method for measurement of an analyte in a microparticle-based analyte-specific binding assay, wherein the microparticles are coated with the first partner of a binding pair, the method involving mixing the coated microparticles, an analyte-specific binding agent conjugated to the second partner of the binding pair, and a sample suspected of containing or containing the analyte, wherein the second partner of the binding pair is bound to the analyte-specific binding agent via a linker having from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agent to the coated microparticles, separating the microparticles having the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.

公开(公告)号：US09834765B2

公开(公告)日：2017-12-05

申请号：US14200325

申请日：2014-03-07

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Frank Bergmann ,  Bruno Frey ,  Dieter Heindl

IPC: C12Q1/68 ,  C12N15/10 ,  C07H21/04

CPC classification number: C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/155 ,  C12Q2525/179 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/16

Abstract: Provided herein is a new class of nucleic acid tagging molecules which are essentially free of homopolymer stretches. The tagging molecules can tag a plurality of individual target molecules for detection with a high degree of accuracy. The tagging molecules can be used to tag at least 105 or 106 individual target molecules. The tagged individual target molecules can be subjected to high throughput sequence analysis.

公开(公告)号：US09796746B2

公开(公告)日：2017-10-24

申请号：US14959280

申请日：2015-12-04

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Dieter Heindl ,  Joachim Hoenes ,  Carina Horn ,  Thomas Meier ,  Wolfgang Petrich

IPC: C07H19/207 ,  G01N33/533 ,  G01N33/535 ,  G01N33/542 ,  G01N33/573 ,  G01N33/58 ,  C12Q1/26 ,  G01N21/64

CPC classification number: C07H19/207 ,  C12Q1/26 ,  G01N21/6428 ,  G01N33/533 ,  G01N33/535 ,  G01N33/542 ,  G01N33/5735 ,  G01N33/581 ,  G01N33/582 ,  G01N2201/062 ,  G01N2333/90248

Abstract: The invention also relates to compounds, which are useful for intra-molecular fluorescence resonance energy transfer (FRET), comprising the oxidized form of a carbaNADH-based first fluorophore and a second fluorophore that is excitable at a wave-length of between 445 to 540 nm and that has an emission maximum of greater than 560 nm, and methods, kits and compositions related thereto.

公开(公告)号：US20170275381A1

公开(公告)日：2017-09-28

申请号：US15465439

申请日：2017-03-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Dieter Heindl ,  Christian Klein ,  Alfred Mertens ,  Volker Schmid ,  Michael Schraemi ,  Monika Soukupova ,  Michael Tacke

IPC: C07K16/46 ,  G01N33/531

CPC classification number: C07K16/468 ,  G01N33/531

Abstract: A bivalent binding agent having a first monovalent binder that binds to a polypeptide epitope of a target polypeptide, a second monovalent binder that binds to a posttranslational polypeptide modification on the target polypeptide and a linker. Further disclosed are methods for the detection of a posttranslationally modified target polypeptide, for making the disclosed bivalent binding agent, and for use of the disclosed bivalent binding agent in histological staining procedures.

公开(公告)号：US20140212903A1

公开(公告)日：2014-07-31

申请号：US14226884

申请日：2014-03-27

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Stacy H. Duvall ,  Peter Gebauer ,  Dieter Heindl ,  Carina Horn ,  Peter Kratzsch ,  Michael Marquant ,  Thomas Meier

IPC: C12Q1/54

CPC classification number: C12Q1/54 ,  C07C281/20 ,  C07D295/215

Abstract: Azo compounds are disclosed that do not form azoxy dimers and that do not have any reactive nitroso groups. Also disclosed are use of the azo compounds as mediators in optical and electrochemical diagnostic methods, as well as detection reagents, kits and test elements that include such azo compounds and that can be used in the diagnostic methods.

Abstract translation: 公开了不形成氮氧化二聚体并且不具有任何反应性亚硝基的偶氮化合物。 还公开了在光学和电化学诊断方法中使用偶氮化合物作为介体，以及包括这种偶氮化合物并且可用于诊断方法的检测试剂，试剂盒和测试元件。

公开(公告)号：US08754111B2

公开(公告)日：2014-06-17

申请号：US13945556

申请日：2013-07-18

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Peter Gebauer ,  Dieter Heindl ,  Carina Horn

IPC: C07D213/08 ,  C07D213/14 ,  C07D211/70 ,  C07D211/82 ,  C07D213/46 ,  A01N43/40 ,  A61K31/44 ,  C07C291/00 ,  C07C257/00 ,  C07C263/00 ,  C07C265/00

CPC classification number: C07F9/58 ,  C07C251/12 ,  C07D213/46

Abstract: A method for the synthesis of N-substituted 3-acylated pyridinium compounds by reacting a pentamethine precursor with a primary amine.

Abstract translation: 通过使五甲胺前体与伯胺反应合成N-取代的3-酰化吡啶鎓化合物的方法。

公开(公告)号：US20230127289A1

公开(公告)日：2023-04-27

申请号：US17990714

申请日：2022-11-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Robert Hahn ,  Dieter Heindl ,  Thomas Hoffmann ,  Hans-Peter Josel ,  Uwe Kobold ,  Hannes Kuchelmeister ,  Simon Ferdinand Loibl ,  Daniela Mazzier ,  Jelena Milic ,  Giuseppe Prencipe ,  Martin Rempt ,  Christoph Seidel

IPC: C07D213/56 ,  C07D401/04 ,  C07D213/74 ,  C07D213/30 ,  H01J49/00

Abstract: The present invention relates to compounds which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said compounds.

公开(公告)号：US10718769B2

公开(公告)日：2020-07-21

申请号：US15186578

申请日：2016-06-20

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans-Peter Josel ,  Dieter Heindl ,  Thomas Froehlich ,  Stefanie Froehner

IPC: C07J17/00 ,  C07J9/00 ,  G01N33/58 ,  C07D311/04 ,  C07D311/82 ,  G01N33/569 ,  C07J51/00 ,  C12N11/06 ,  G01N33/50 ,  G01N33/543 ,  C07D495/04

Abstract: The present invention relates to novel compounds comprising one or more hydrophobic domains and a hydrophilic domain comprising PEG moieties, useful for binding cells, as well as uses and compositions related thereto. The compounds are useful for immobilizing and/or stabilizing cells.

公开(公告)号：US20190293612A1

公开(公告)日：2019-09-26

申请号：US16438797

申请日：2019-06-12

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Dieter Heindl ,  Thomas Heinemeyer ,  Uwe Kobold ,  Martin Rempt ,  Christoph Seidel

IPC: G01N30/46 ,  G01N30/06 ,  G01N30/72 ,  G01N33/58

Abstract: A method for identifying a reagent during a process in an analysis system is disclosed. The analysis system comprises a liquid chromatograph and a mass spectrometer. The method comprises providing a reagent, adding at least one chemical substance to the reagent with a concentration being above a detection level of the mass spectrometer, processing the reagent together with the chemical substance with the analysis system, and identifying the reagent based on a detection of a substance detection signal of the mass spectrometer representing the chemical substance.