公开(公告)号：USD342575S

公开(公告)日：1993-12-21

申请号：US855933

申请日：1992-03-23

申请人： Yoshihiro Ashihara ,  Mitsushi Gotanda ,  Akira Hasegawa ,  Nobuyuki Takahashi

设计人： Yoshihiro Ashihara ,  Mitsushi Gotanda ,  Akira Hasegawa ,  Nobuyuki Takahashi

公开(公告)号：US5093081A

公开(公告)日：1992-03-03

申请号：US369332

申请日：1989-06-21

申请人： Yukio Sudo ,  Yoshihiro Ashihara ,  Toshikage Hiraoka ,  Isao Nishizono ,  Shigeki Kageyama ,  Tetsuji Tanimoto

发明人： Yukio Sudo ,  Yoshihiro Ashihara ,  Toshikage Hiraoka ,  Isao Nishizono ,  Shigeki Kageyama ,  Tetsuji Tanimoto

IPC分类号： G01N33/543

CPC分类号： G01N33/54386

摘要： A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-insoluble macromolecular substrate, and(B) an antibody, reacting with the ligand in the sample, conjugated with an enzyme capable of acting on the above water-insoluble macromolecular substrate.The above analytical substance further comprises,(C) a macromolecular substance which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct a highly sensitive and simple enzyme immunoassay.

公开(公告)号：US06251618B1

公开(公告)日：2001-06-26

申请号：US09417220

申请日：1999-10-13

申请人： Masami Sugiyama ,  Mamoru Miyazaki ,  Yoshihiro Ashihara

发明人： Masami Sugiyama ,  Mamoru Miyazaki ,  Yoshihiro Ashihara

IPC分类号： G01N33543

CPC分类号： G01N33/581 ,  Y10S435/962

摘要： Disclosed are a color developing method utilizing the reaction of an indolyl derivative with an enzyme in the presence of a specific free radical compound and/or a specific chelate compound, and an enzyme immunoassay using the color developing method.

摘要翻译： 公开了利用吲哚基衍生物与酶在特定自由基化合物和/或特定螯合物的存在下的反应的彩色显影方法，以及使用彩色显影法的酶免疫测定法。

公开(公告)号：US06221625B1

公开(公告)日：2001-04-24

申请号：US09017214

申请日：1998-02-02

申请人： Yoshihiro Ashihara ,  Mitsuo Isomura ,  Atsuka Sato

发明人： Yoshihiro Ashihara ,  Mitsuo Isomura ,  Atsuka Sato

IPC分类号： G01N3353

CPC分类号： G01N33/581 ,  G01N33/542 ,  Y10S435/97 ,  Y10S436/81

摘要： An enzyme-labeled immunoassay is performed by the steps of allowing a test sample to react with an enzyme-labeled reagent, allowing a substrate to react with the enzyme to form a signal, and immobilising the enzyme-labeled reagent, with the prevention of a further signal formation from a predetermined time on after the immobilisation of the enzyme-labeled reagent, using an enzyme inhibitor. A device for performing this enzyme-labeled immunoassay includes an absorbent material capable of transporting a developing solution by capillary action, the absorbent material including (a) a developing liquid application zone, (b) an enzyme-labeled reagent zone containing an enzyme-labeled reagent, (c) a sample receiving zone, and (d) an indicator reagent zone capable of immobilising the enzyme-labeled reagent after the reaction of the test sample with the enzyme-labeled reagent in an amount dependent on the assay result, with an enzyme inhibitor being applied to a portion in the absorbent material upstream of the enzyme-labeled reagent zone, which enzyme inhibitor prevents the formation of a signal from a predetermined time on after the enzyme-labeled reagent is immobilised at the indicator reagent zone.

摘要翻译： 通过以下步骤进行酶标记的免疫测定：使测试样品与酶标记的试剂反应，允许底物与酶反应以形成信号，并固定酶标记的试剂，同时防止 使用酶抑制剂从固定酶标记的试剂之后的预定时间进一步形成信号。 用于进行该酶标记免疫测定的装置包括能够通过毛细作用输送显影液的吸收材料，所述吸收材料包括（a）显影液体施用区，（b）酶标记的试剂区，其含有酶标记的 试剂，（c）样品接收区，和（d）指示剂试剂区，其能够在测试样品与酶标记的试剂反应后以取决于测定结果的量固定酶标记的试剂， 酶抑制剂被施加到酶标记试剂区上游的吸收材料的一部分，该酶抑制剂防止在酶标记的试剂固定在指示剂试剂区之后的预定时间内形成信号。

公开(公告)号：US5736349A

公开(公告)日：1998-04-07

申请号：US440175

申请日：1995-05-12

申请人： Motohiro Sasaki ,  Masahiko Matsukawa ,  Katsuaki Yoshioka ,  Makoto Anan ,  Mitsuo Isomura ,  Yoshihiro Ashihara ,  Masahisa Okada

发明人： Motohiro Sasaki ,  Masahiko Matsukawa ,  Katsuaki Yoshioka ,  Makoto Anan ,  Mitsuo Isomura ,  Yoshihiro Ashihara ,  Masahisa Okada

IPC分类号： B03C1/01 ,  G01N33/543 ,  G01N33/553 ,  C07K17/14

CPC分类号： B03C1/01 ,  G01N33/5434 ,  G01N2446/10 ,  G01N2446/86 ,  G01N2446/90

摘要： There are disclosed a magnetic particle for an immunoassay method, which comprises a core and a coating layer formed on the surface of the core wherein said core comprises an organic polymer matrix and said coating layer comprises a mixed crystal ferrite represented by the formula: M.sub.x Fe.sub.(3-x) O.sub.4 wherein M represents at least one metal selected from the group consisting of Mn, Ni, Zn, Co, Cu, Mg, Sn, Ca and Cd, and x is a number satisfying the relation: 0

摘要翻译： 公开了一种用于免疫测定方法的磁性颗粒，其包括芯和形成在芯的表面上的涂层，其中所述芯包含有机聚合物基质，所述涂层包含由下式表示的混合晶体铁氧体：MxFe（ 3-x）O 4，其中M表示选自Mn，Ni，Zn，Co，Cu，Mg，Sn，Ca和Cd中的至少一种金属，x是满足以下关系的数：0

公开(公告)号：US5603898A

公开(公告)日：1997-02-18

申请号：US993964

申请日：1992-12-17

申请人： Yoshihiro Ashihara ,  Yukio Sudo ,  Isao Nishizono ,  Toshikage Hiraoka ,  Tetsuji Tanimoto ,  Shigeki Kageyama

发明人： Yoshihiro Ashihara ,  Yukio Sudo ,  Isao Nishizono ,  Toshikage Hiraoka ,  Tetsuji Tanimoto ,  Shigeki Kageyama

IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N21/00 ,  C07K16/00 ,  C12M1/14 ,  G01N33/53

CPC分类号： G01N33/581 ,  G01N33/54386

摘要： A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,(A) a water-soluble macromolecular substrate, and(B) an enzyme conjugated with antibody reacting with the ligand in the sample capable to act on the above water-soluble macromolecular substrate. The above analytical element further comprises,(C) a macromolecular antigen which is a conjugate of the ligand or its derivative with a macromolecular compound in the above water-permeable layer of the above dry-type analytical element.The invention can conduct highly sensitive and simple enzyme immunoassay.

摘要翻译： 一种用于免疫测定的干式分析元件，其具有至少一个用于根据酶免疫测定法测量样品中的配体的透水层，其包含（A）水溶性大分子底物和（B）与抗体结合的酶 与能够作用于上述水溶性大分子底物的样品中的配体反应。 上述分析元素还包含（C）在上述干式分析元件的上述透水层中作为配体或其衍生物与高分子化合物的共轭物的高分子抗​​原。 本发明可以进行高灵敏度和简单的酶免疫测定。

公开(公告)号：US5569589A

公开(公告)日：1996-10-29

申请号：US163405

申请日：1993-12-07

申请人： Toshikage Hiraoka ,  Tetsuji Tanimoto ,  Yoshihiko Makino ,  Tadashi Ninomiya ,  Naofumi Hora ,  Yoshihiro Ashihara ,  Yukio Sudo ,  Toshihiro Mori

发明人： Toshikage Hiraoka ,  Tetsuji Tanimoto ,  Yoshihiko Makino ,  Tadashi Ninomiya ,  Naofumi Hora ,  Yoshihiro Ashihara ,  Yukio Sudo ,  Toshihiro Mori

IPC分类号： G01N31/22 ,  G01N33/542 ,  G01N33/543 ,  G01N33/53

CPC分类号： G01N33/54386 ,  Y10S435/966 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/807 ,  Y10S436/81

摘要： An immunoassay element for quantitatively analyzing a ligand by determining the change in enzymatic activity caused by a reaction between the ligand, a linked product of the ligand and a high molecular weight compound and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the non-difussible material. Also provided is a process for quantitatively analyzing a ligand contained in a sample by the use of the immunoassay element.

摘要翻译： 一种免疫测定元件，用于通过测定配体，配体与高分子化合物的连接产物和酶标记的抗体之间的反应引起的酶活性的变化来定量分析配体。 免疫测定元件包括含有在酶存在下形成可扩散材料的非扩散性底物的底物层和含有用于进一步碎裂非可弥散材料的碎裂酶的试剂层。 还提供了通过使用免疫测定元件定量分析样品中所含的配体的方法。

公开(公告)号：US5482839A

公开(公告)日：1996-01-09

申请号：US57503

申请日：1993-05-04

申请人： Yoshihiro Ashihara ,  Isao Nishizono ,  Hidetaka Minakawa ,  Masahisa Okada ,  Yasusuke Sakurabayashi ,  Fumio Watanabe ,  Shin-ichi Wakana

发明人： Yoshihiro Ashihara ,  Isao Nishizono ,  Hidetaka Minakawa ,  Masahisa Okada ,  Yasusuke Sakurabayashi ,  Fumio Watanabe ,  Shin-ichi Wakana

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G01N33/553

CPC分类号： G01N33/53 ,  G01N33/54326 ,  G01N35/02 ,  G01N2035/00534 ,  G01N2035/0465 ,  G01N2035/0498 ,  G01N35/0098 ,  G01N35/1079 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/113332 ,  Y10T436/114998 ,  Y10T436/119163

摘要： An automatic immunoassay apparatus utilizes cartridges each having at least two wells, a first well of said wells containing solid phase material carrying antigen or antibody, a second well of said wells containing antibody or antigen labelled with labelling compound. The wells may be sealed with a suitable sealing film before use and the sealing film is broken when the cartridge is to be used. The cartridges are transported to a predetermined position on a steppingly movable reaction line and conveyed thereby at a predetermined interval. While the reaction line steps, a sample, labelled antigen or antibody contained in the second well and substrate, if necessary, are added to the first well and stirred at predetermined timings and reaction between the sample and a reactive solution is measured under control of a control device having memory means storing operator selectable programs for various measuring methods.

摘要翻译： 自动免疫测定装置使用各自具有至少两个孔的柱，所述孔的第一孔包含携带抗原或抗体的固相材料，所述孔的第二孔含有用标记化合物标记的抗体或抗原。 在使用之前，孔可以用合适的密封膜密封，并且当使用盒时密封膜破裂。 药筒被输送到步进可移动的反应线上的预定位置，并以预定间隔被输送。 当反应线步骤时，如果需要，将第二阱和底物中包含的样品，标记的抗原或抗体加入到第一阱中并在预定的时间进行搅拌，并在样品和反应溶液之间进行反应，在 具有存储装置的控制装置存储用于各种测量方法的操作者可选程序。

公开(公告)号：US5320944A

公开(公告)日：1994-06-14

申请号：US965612

申请日：1992-10-22

申请人： Masahisa Okada ,  Yoshihiro Ashihara ,  Akira Yano ,  Masao Oishi ,  Katsuaki Yoshioka ,  Toshiomi Nakamura

发明人： Masahisa Okada ,  Yoshihiro Ashihara ,  Akira Yano ,  Masao Oishi ,  Katsuaki Yoshioka ,  Toshiomi Nakamura

IPC分类号： G01N33/545 ,  B03C1/01 ,  G01N33/543 ,  G01N33/553 ,  G01N33/535

CPC分类号： B03C1/01 ,  G01N33/5434 ,  G01N2446/10 ,  G01N2446/86 ,  G01N2446/90 ,  Y10S435/968 ,  Y10S436/806

摘要： An immunoassay method using magnetic particles comprising a core and a coating layer on the surface thereof, The core comprises an organic polymer and the coating layer comprises an iron oxide type ferrite coating layer. An antigen or an antibody is bound onto the surface of the coating layer and the particle has a particle size of 0.2 to 3 .mu.m.

摘要翻译： 使用在其表面上包含芯和涂层的磁性颗粒的免疫测定方法。芯包含有机聚合物，并且涂层包含氧化铁类铁氧体涂层。 抗原或抗体结合在涂层的表面上，粒子的粒径为0.2〜3μm。

公开(公告)号：US5094939A

公开(公告)日：1992-03-10

申请号：US382112

申请日：1989-07-19

申请人： Masahisa Okada ,  Yoshihiro Ashihara ,  Tadashi Ninomiya ,  Akira Yano

发明人： Masahisa Okada ,  Yoshihiro Ashihara ,  Tadashi Ninomiya ,  Akira Yano

IPC分类号： C07F9/655 ,  C12Q1/42 ,  C12Q1/68

CPC分类号： C12Q1/42 ,  C07F9/65512 ,  C12Q1/6816 ,  C12Q2334/00 ,  Y10S436/80 ,  Y10S436/805

摘要： A multi-pH chemiluminescence-based assay method comprising first-stage light production by the activation by cleavage of a phosphatase cleavable dioxetane derivative within a pH range optimal for enzyme catalysis, followed by the second-stage generation of increased light energy from the products of enzyme-catalyzed cleavage of the dioxetane derivative by the adjustment of the pH to a strongly alkaline pH optimal for the generation of light energy. The assay method is suitable for both solution phase and solid state assay formats. Light generation enhancers can be used further to increase the light yield.

摘要翻译： 一种基于多pH化学发光的测定方法，其包括通过在最适于酶催化的pH范围内切割磷酸酶裂解的二氧杂环丁烷衍生物进行激活的第一阶段光产生，随后是来自产物的第二阶段产生增加的光能 通过将pH调节至最适于产生光能的强碱性pH的二氧杂环丁烷衍生物的酶催化裂解。 测定方法适用于溶液相和固态分析方法。 光产生增强剂可以进一步用于增加光产率。