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    Stable reagents and kits useful in loop-mediated isothermal amplification (LAMP)
    21.
    发明申请
    Stable reagents and kits useful in loop-mediated isothermal amplification (LAMP) 审中-公开
    可用于环介导等温扩增（LAMP）的稳定试剂和试剂盒

    公开(公告)号：US20080182312A1

    公开(公告)日：2008-07-31

    申请号：US12009183

    申请日：2008-01-16

    申请人： Todd Denison Pack ,  Xiaokang Deng

    发明人： Todd Denison Pack ,  Xiaokang Deng

    IPC分类号： C12N9/00

    CPC分类号： C12Q1/6844 ,  C12Q2565/625 ,  C12Q2531/119

    摘要： Provided herein is a reagent preparation for loop-mediated isothermal amplification of nucleic acids comprising: at least one polymerase enzyme, a target-specific primer set, and dinucleotide triphosphates (dNTPs) in a single, dry format; wherein said reagent preparation is water soluble and stable above 4° C.

    摘要翻译： 本文提供了一种用于环介导的核酸等温扩增的试剂制剂，其包括：单一干燥形式的至少一种聚合酶，靶特异性引物组和二核苷酸三磷酸（dNTP）; 其中所述试剂制剂在4℃以上是水溶性且稳定的。

    CAPTURE AND DETECTION FORMAT VERSATILITY FOR DIPSTICK ASSAYS
    22.
    发明申请
    CAPTURE AND DETECTION FORMAT VERSATILITY FOR DIPSTICK ASSAYS 审中-公开
    捕获和检测用于DIPSTICK测定的形式多样性

    公开(公告)号：US20080160516A1

    公开(公告)日：2008-07-03

    申请号：US11686580

    申请日：2007-03-15

    申请人： Helen LEE ,  Hsiang Yun Hu ,  Magda Anastassova Dineva

    发明人： Helen LEE ,  Hsiang Yun Hu ,  Magda Anastassova Dineva

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6834 ,  C12Q1/689 ,  C12Q2565/625

    摘要： Dipsticks for testing for the presence of a target nucleic acid in a sample solution are described the dipsticks comprise a universal capture probe immobilised at a capture zone of the dipstick. The universal capture probe is capable of hybridising to a hook capture probe which is hybridised to the target nucleic acid in the sample solution. A contact end of the dipstick is contacted with the sample solution to cause hook capture probe hybridised to the target nucleic acid to move by capillary action to the capture zone where the target nucleic acid can be detected. Use of the universal and hook capture probes allows dipsticks to be prepared which can be used to capture any target nucleic acid, thereby simplifying preparation of the dipsticks. Specificity of target nucleic acid capture is then achieved by use of an appropriate hook capture probe. Methods and kits are also described.

    摘要翻译： 描述了用于测试样品溶液中目标核酸存在的浸渍条，浸渍条包括固定在量油尺的捕获区的通用捕获探针。 通用捕获探针能够与钩状捕获探针杂交，其与样品溶液中的靶核酸杂交。 将量油尺的接触端与样品溶液接触，使钩捕捉探针与目标核酸杂交，通过毛细管作用移动到可以检测靶核酸的捕获区。 使用通用和钩捕获探针可以制备可以用于捕获任何靶核酸的骰子，从而简化骰子的制备。 然后通过使用适当的钩捕获探针实现靶核酸捕获的特异性。 还描述了方法和试剂盒。

    LATERAL FLOW DEVICES
    23.
    发明申请
    LATERAL FLOW DEVICES 有权
    侧向流动装置

    公开(公告)号：US20070269821A1

    公开(公告)日：2007-11-22

    申请号：US11686601

    申请日：2007-03-15

    申请人： Debapriya Mazumdar ,  Juewen Liu ,  Yi Lu

    发明人： Debapriya Mazumdar ,  Juewen Liu ,  Yi Lu

    IPC分类号： C12Q1/68 ,  G01N21/01 ,  G01N33/00

    CPC分类号： C12Q1/6834 ,  C12Q2565/625 ,  C12Q2521/301 ,  C12Q2525/205

    摘要： An analytical test for an analyte comprises (a) a base, having a reaction area and a visualization area, (b) a capture species, on the base in the visualization area, comprising nucleic acid, and (c) analysis chemistry reagents, on the base in the reaction area. The analysis chemistry reagents comprise (i) a substrate comprising nucleic acid and a first label, and (ii) a reactor comprising nucleic acid. The analysis chemistry reagents can react with a sample comprising the analyte and water, to produce a visualization species comprising nucleic acid and the first label, and the capture species can bind the visualization species.

    摘要翻译： 分析物的分析测试包括（a）具有反应区域和可视化区域的碱，（b）可视区域的基底上的捕获物质，其包含核酸，和（c）分析化学试剂，在 在反应区的基地。 分析化学试剂包含（i）包含核酸和第一标记的底物，和（ii）包含核酸的反应器。 分析化学试剂可以与包含分析物和水的样品反应，以产生包含核酸和第一标记的可视物质，并且捕获物质可以结合可视物种。

    CAPTURE AND DETECTION OF TARGET NUCLEIC ACID IN DIPSTICK ASSAYS
    24.
    发明申请
    CAPTURE AND DETECTION OF TARGET NUCLEIC ACID IN DIPSTICK ASSAYS 有权
    捕获和检测目标核酸在DIPSTICK测定中的检测

    公开(公告)号：US20070190548A1

    公开(公告)日：2007-08-16

    申请号：US11624861

    申请日：2007-01-19

    申请人： Helen LEE ,  Magda Dineva ,  Shaun Hazlewood

    发明人： Helen LEE ,  Magda Dineva ,  Shaun Hazlewood

    IPC分类号： C12Q1/68 ,  C12M3/00 ,  C07H21/04

    CPC分类号： C12Q1/6834 ,  G01N30/02 ,  G01N30/94 ,  C12Q2565/625 ,  C12Q2537/162 ,  B01D15/3804

    摘要： Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is contacted with the contact end of the dipstick to cause the sample solution to move by capillary action to a capture zone of the dipstick at which target nucleic acid is captured. The target nucleic acid may be captured at the capture zone by a capture probe capable of hybridising to the target nucleic acid. A labelled detection probe capable of hybridising to the target nucleic acid may be used to detect the target nucleic acid at the capture zone. A helper probe may be used to enhance the binding of the capture and/or detection probe to the target nucleic acid, thereby improving the sensitivity of target nucleic acid detection. Dipsticks and kits are also described.

    摘要翻译： 描述了在试纸测定中使用辅助探针。 在用于测试样品溶液中目标核酸的存在的试纸测试中，将样品溶液与量油尺的接触端接触，以使样品溶液通过毛细作用移动到量油尺的捕获区， 捕获靶核酸。 可以通过能够与靶核酸杂交的捕获探针在捕获区捕获靶核酸。 能够与靶核酸杂交的标记检测探针可用于检测捕获区的靶核酸。 可以使用辅助探针来增强捕获和/或检测探针与靶核酸的结合，从而提高靶核酸检测的灵敏度。 还描述了骰子和工具包。

    Nucleic acid detection system
    29.
    发明申请
    Nucleic acid detection system 审中-公开
    核酸检测系统

    公开(公告)号：US20050227275A1

    公开(公告)日：2005-10-13

    申请号：US11102001

    申请日：2005-04-07

    申请人： Jaean Jung ,  Young Choi ,  Youngsun Kim

    发明人： Jaean Jung ,  Young Choi ,  Youngsun Kim

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/701 ,  C12Q1/6816 ,  C12Q1/6834 ,  Y02A50/53 ,  C12Q2565/625 ,  C12Q2563/131 ,  C12Q2537/125 ,  C12Q2565/137 ,  C12Q2545/101

    摘要： The present application discloses a system for detecting target nucleic acid comprising: a container comprising a nucleic acid amplification mix comprising a primer labeled with different haptens at its 5′ and 3′ ends, and optionally dNTP labeled with a hapten to form a nucleic acid complex; and a lateral flow test device comprising a reservoir area comprising reagent conditions suitable for binding of a specific binding partner with the nucleic acid complex; a dye area comprising a specific binding partner to the nucleic acid complex, wherein the specific binding partner is linked or conjugated to a reporter dye or another hapten; and a test area comprising a different specific binding partner specific to a different aspect of the nucleic acid complex.

    摘要翻译： 本申请公开了一种用于检测靶核酸的系统，包括：容器，其包含核酸扩增混合物，其包含在其5'和3'末端用不同半抗原标记的引物，以及任选地用半抗原标记的dNTP以形成核酸复合物 ; 和侧流测试装置，其包括储存区域，其包含适合于将特异性结合配偶体与核酸复合物结合的试剂条件; 染料区域，其包含与所述核酸复合物的特异性结合配偶体，其中所述特异性结合配偶体与报告染料或其他半抗原连接或缀合; 以及包含对核酸复合物的不同方面特异的不同特异性结合伴侣的测试区域。