公开(公告)号：US09598707B2

公开(公告)日：2017-03-21

申请号：US13784877

申请日：2013-03-05

申请人： Arkansas State University

发明人： Luis Fabricio Medina-Bolivar ,  Tianhong Yang

IPC分类号： C12P33/20 ,  C12P17/06 ,  C12P17/12 ,  C12P7/22 ,  C12P19/02 ,  C12N5/00 ,  C12N5/04 ,  C12P17/18 ,  C12P19/46 ,  C12P17/04

CPC分类号： C12P7/22 ,  C12N5/0025 ,  C12N5/04 ,  C12P17/04 ,  C12P17/06 ,  C12P17/188 ,  C12P19/46 ,  Y02A40/14

摘要： A method to increase the production of products of interest in plant material including plant cultures, such as, for example, cell suspension cultures, root cultures, and hairy root cultures is provided. In one embodiment, the method is to contacting the plant material with a precursor or xenobiotic when producing a product of interest from a plant. In another embodiment the plant material is also contacted with a trapping agent. The process may also provide for contacting an elicitor of the product of interest with the plant material. An embodiment provides for contacting an elicitor, precursor and trapping agent with the plant material. The ability to produce novel compounds such as glucosides and glucuronides is provided.

公开(公告)号：US20160083767A1

公开(公告)日：2016-03-24

申请号：US14857703

申请日：2015-09-17

申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

发明人： Sun-Chang KIM ,  Giltsu CHOI ,  Suk-Chae JUNG ,  Woohyun KIM ,  Soohwan LIM ,  Wan-Taek IM

IPC分类号： C12P33/20 ,  C12N9/10

CPC分类号： C12P33/20 ,  C12N9/1051 ,  C12P19/56 ,  C12P33/00 ,  C12Y204/01

摘要： Provided are a novel UDP-glycosyltransferase (uridine diphosphate glycosyltransferase) protein having glycosyltransfer activity for glucose linked by a glycosidic bond at the C-20 position of PPD (protopanaxadiol)-type or PPT (protopanaxatriol)-type ginsenoside, and use thereof.

摘要翻译： 提供了一种具有通过PPD（原人参二醇）型或PPT（原人参三醇）型人参皂苷的C-20位上的糖苷键连接的葡萄糖的糖基转移酶的新型UDP-糖基转移酶（尿苷二磷酸糖基转移酶）蛋白及其应用。

公开(公告)号：US20150184216A1

公开(公告)日：2015-07-02

申请号：US14588790

申请日：2015-01-02

申请人： AMOREPACIFIC CORPORATION

发明人： Jun Seong PARK ,  Duck Hee KIM ,  Han Kon KIM ,  Eun Mi KIM ,  Byung Gee KIM

IPC分类号： C12P33/20 ,  C12P17/06

CPC分类号： C12P33/20 ,  C12N1/20 ,  C12N9/0004 ,  C12P17/06 ,  C12P19/02 ,  C12P33/00 ,  C12R1/41 ,  Y02P20/52

摘要： The present invention relates to the novel Rhizobium sp. GIN611 KCTC11708BP or to cell extracts thereof, to a novel oxidoreductase which exhibits a glycolytic activity, to a gene encoding the oxidoreductase, to a recombinant strain comprising recombinant vector proteins or to an expression vector encoding recombinant proteins, and to a method for the glycolysis of natural products using same as a biocatalyst. The present invention also relates to a method for producing aglycones from a variety of natural products using same. The novel oxidoreductase separated from the novel microorganism of the present invention does not belong to a glucosidase group but belongs to an oxidoreductase group, and has a glycolytic activity for natural products. The novel oxidoreductase oxidizes the sugar in the aglycones of natural products, thereby producing a variety of aglycones.

摘要翻译： 本发明涉及新根瘤菌属（Rhizobium sp。） GIN611KCTC11708BP或其细胞提取物，对具有糖酵解活性的新型氧化还原酶，与编码该氧化还原酶的基因相连，包含重组载体蛋白的重组菌株或编码重组蛋白的表达载体，以及糖酵解方法 使用与生物催化剂相同的天然产物。 本发明还涉及使用它们的各种天然产物制备糖苷配基的方法。 从本发明的新型微生物分离的新型氧化还原酶不属于葡萄糖苷酶基团，属于氧化还原酶基团，对天然产物具有糖酵解活性。 新型氧化还原酶氧化天然产物的糖苷配基中的糖，从而产生各种糖苷配基。

公开(公告)号：US20120297507A1

公开(公告)日：2012-11-22

申请号：US13394845

申请日：2010-08-12

申请人： Franck Michoux ,  Peter Nixon ,  James Gerard McCarthy

发明人： Franck Michoux ,  Peter Nixon ,  James Gerard McCarthy

IPC分类号： C12N5/04 ,  C12P21/00 ,  C12N9/00 ,  C07K14/00 ,  C07K14/475 ,  C07K14/575 ,  C07K16/00 ,  A01H5/12 ,  A61K36/18 ,  C12P7/06 ,  C12P7/16 ,  C12P7/04 ,  C11C3/10 ,  C07C31/08 ,  C07C31/10 ,  C07C31/12 ,  C12P1/00 ,  C12P19/00 ,  C12P7/64 ,  C12P5/00 ,  C12P17/18 ,  C12P33/20 ,  C12P13/00 ,  C12P17/02 ,  A23L1/28 ,  C02F3/32 ,  C12N5/10

CPC分类号： C12N15/8257 ,  A01H4/00 ,  A01H4/005 ,  A01H4/008 ,  A23L19/00 ,  A23V2002/00 ,  A61K36/81 ,  C10L1/02 ,  C10L1/1822 ,  C10L2200/0469 ,  C10L2200/0476 ,  C12N15/8209 ,  C12N15/8214 ,  C12N15/8225 ,  Y02E50/17 ,  Y02P60/247

摘要： A method for producing leafy biomass from undifferentiated plant cells, the method comprising providing undifferentiated plant cells, contacting them with an agent that promotes differentiation of the cells into leafy tissue and growing the cells in a temporary liquid immersion culture system. This method of the invention may be used to produce polypeptides, and natural medicinal products, and can be used to capture carbon dioxide. A method of producing a polypeptide in plant cells in vitro comprising: providing undifferentiated plant cells containing chloroplasts that carry a transgenic nucleic acid molecule encoding the polypeptide, wherein the plant cells display homoplastomy; and propagating the cells according to the above method to produce leafy biomass containing the polypeptide.

摘要翻译： 一种从未分化植物细胞产生叶绿体生物质的方法，所述方法包括提供未分化的植物细胞，使其与促进细胞分化为叶状组织并在临时液体浸渍培养系统中生长细胞的试剂接触。 本发明的该方法可用于产生多肽和天然药用产品，并且可用于捕获二氧化碳。 一种在体外在植物细胞中产生多肽的方法，其包括：提供含有携带编码所述多肽的转基因核酸分子的叶绿体的未分化植物细胞，其中所述植物细胞显示均匀切除术; 并根据上述方法繁殖细胞以产生含有该多肽的叶状生物质。

公开(公告)号：US06911325B1

公开(公告)日：2005-06-28

申请号：US09979106

申请日：2000-05-29

申请人： Cesare Ponzone ,  Mario De Rosa ,  Alessandra Morana ,  Antonella Di Lazzaro

发明人： Cesare Ponzone ,  Mario De Rosa ,  Alessandra Morana ,  Antonella Di Lazzaro

IPC分类号： C12P33/20 ,  C12P19/44 ,  C12P33/00 ,  C12R1/685

CPC分类号： C12P33/00

摘要： A process for the preparation of desglucodesrhamnoruscin which comprises the enzymatic hydrolysis of Ruscus Aculeatus steroid glycosides (ruscosaponins) by means of crude hydrolases from aspergillus niger.

摘要翻译： 一种制备葡糖酸芽孢杆菌素的方法，其包括通过来自黑曲霉的粗水解酶酶促水解芸苔属甾体糖苷（ruscosaponin）。

公开(公告)号：US4431732A

公开(公告)日：1984-02-14

申请号：US334025

申请日：1981-12-23

申请人： Alfred Weber ,  Mario Kennecke ,  Rudolf Muller

发明人： Alfred Weber ,  Mario Kennecke ,  Rudolf Muller

IPC分类号： C07J5/00 ,  C07J71/00 ,  C12P33/02 ,  C12P33/20 ,  C12R1/06

CPC分类号： C12P33/02 ,  C07J71/0068

摘要： A process for preparing 11.beta.,21-dihydroxy-2'-methyl-5'.beta.H-1,4-pregnadieno(16,17-d)-oxazole-3,20-dione, comprises fermenting 11.beta.,21-dihydroxy-2'-methyl-5'.beta.H-4-pregneno (16,17-d)-oxazole-3,20-dione with a living culture of Arthrobacter simplex.

摘要翻译： 制备11β，21-二羟基-2'-甲基-5'β-H-1,4-孕孕二烯（16,17-d） - 恶唑-3,20-二酮的方法包括发酵11β，21-二羟基 -2'-甲基-5'β-H-4-孕烯（16,17-d） - 恶唑-3,20-二酮与单节节杆菌的活体培养物。

公开(公告)号：US4416985A

公开(公告)日：1983-11-22

申请号：US317822

申请日：1981-11-03

申请人： Karl Petzoldt ,  Rudolf Wiechert ,  Henry Laurent ,  Klaus Nickisch ,  Dieter Bittler

发明人： Karl Petzoldt ,  Rudolf Wiechert ,  Henry Laurent ,  Klaus Nickisch ,  Dieter Bittler

IPC分类号： C07J21/00 ,  C07J53/00 ,  C07J71/00 ,  C12P33/06 ,  C12P33/20 ,  C12R1/645 ,  C07J7/00

CPC分类号： C12P33/06 ,  C07J53/008 ,  C07J71/001

摘要： A process for preparing a 3.beta.,7.beta.-dihydroxy-.DELTA..sup.5 steroid of the formula ##STR1## wherein Q is ##STR2## and R.sub.1 is hydrogen, trimethylacetyl, tert-butyldimethylsilyl, dimethyl-2-(3-methylbutyl)silyl or tribenzylsilyl,comprises fermenting a 3.beta.-hydroxy-.DELTA..sup.5 -steroid of the formula ##STR3## wherein Q is as defined above, andR.sub.2 is hydrogen or alkanoyl of 2-6 carbon atoms,with a culture of Botryodiplodia malorum to obtain the corresponding 3.beta.,7.beta.-dihydroxy-.DELTA..sup.5 -steroid; and, optionally, reacting the resultant product with trimethylacetic anhydride, tert-butyldimethylsilyl chloride, dimethyl-2-(3-methylbutyl)silyl chloride, or tribenzylsilyl chloride.

摘要翻译： 制备式“IMAGE”的3β，7β-二羟基-TATA 5类的方法，其中Q是R 1，R 1是氢，三甲基乙酰基，叔丁基二甲基甲硅烷基，二甲基-2-（3-甲基丁基）甲硅烷基或三苄基甲硅烷基 包括发酵式为“IMAGE”的3β-羟基-TATA5-类固醇，其中Q如上所定义，R 2为2-6个碳原子的氢或链烷酰基，并具有葡萄孢属（Botryodiplodia malorum）的培养物，以获得相应的3 β，β-二羟基-DTATA5-类固醇; 和任选地使所得产物与三甲基乙酸酐，叔丁基二甲基甲硅烷基氯，二甲基-2-（3-甲基丁基）甲硅烷基氯或三苄基甲硅烷基氯反应。

公开(公告)号：US4360595A

公开(公告)日：1982-11-23

申请号：US236381

申请日：1981-02-20

申请人： David M. Balitz ,  James A. Bush ,  Frances A. O'Herron ,  Donald E. Nettleton, Jr.

发明人： David M. Balitz ,  James A. Bush ,  Frances A. O'Herron ,  Donald E. Nettleton, Jr.

IPC分类号： C12P33/20 ,  A61K31/58 ,  A61P31/04 ,  A61P35/02 ,  C07D407/04 ,  C07J73/00 ,  C12P17/16 ,  C12P33/00 ,  C12R1/465 ,  C12P17/18

CPC分类号： C07D407/04 ,  C12P17/162 ,  C12R1/465

摘要： A polycyclic antitumor antibiotic designated herein as anandimycin is produced by fermentation of Streptomyces anandii subsp. araffinosus strain C-22,437 (ATCC 31431). Anandimycin possesses antibacterial activity and inhibits the growth of tumors in experimental animals.

摘要翻译： 本文称为阿霉素的多环抗肿瘤抗生素通过发酵Anandii subsp。 花椰菜菌株C-22,437（ATCC 31431）。 阿那霉素具有抗菌活性，抑制实验动物肿瘤的生长。

公开(公告)号：US20240158451A1

公开(公告)日：2024-05-16

申请号：US18281479

申请日：2022-03-11

申请人： Ginkgo Gioworks, Inc.

发明人： Diveena Becker ,  Josef Bober ,  Justin Michael Gardin ,  Matthew McMahon

IPC分类号： C07K14/415 ,  C12N9/02 ,  C12N9/14 ,  C12N9/90 ,  C12N15/52 ,  C12P19/56 ,  C12P33/20

CPC分类号： C07K14/415 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/14 ,  C12N9/90 ,  C12N15/52 ,  C12P19/56 ,  C12P33/20 ,  C12Y114/14001 ,  C12Y114/99007 ,  C12Y303/02003 ,  C12Y504/99007 ,  C12Y504/99033

摘要： Described in this application are proteins and host cells involved in methods of producing mogrol precursors, mogrol, and/or mogrosides.

公开(公告)号：US20220170063A1

公开(公告)日：2022-06-02

申请号：US17563127

申请日：2021-12-28

申请人： The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization

发明人： Maxim ITKIN ,  Rachel DAVIDOVICH-RIKANATI ,  Shahar COHEN ,  Vitaly PORTNOY ,  Adi DORON-FAIGENBOIM ,  Marina PETREIKOV ,  Shmuel SHEN ,  Yaakov TADMOR ,  Yosef BURGER ,  Efraim LEWINSOHN ,  Nurit KATZIR ,  Arthur A. SCHAFFER ,  Elad OREN

IPC分类号： C12P33/12 ,  A23L27/30 ,  C12N9/90 ,  A23L2/60 ,  C12N9/14 ,  C12P33/20 ,  C12P19/56 ,  C07J17/00 ,  C12N9/02 ,  C12N9/88

摘要： Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.