Endoglucanases
    31.
    发明申请
    Endoglucanases 有权
    内切葡聚糖酶

    公开(公告)号:US20050070003A1

    公开(公告)日:2005-03-31

    申请号:US10965499

    申请日:2004-10-14

    摘要: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) ThrArgXaaXaaAspCysCysXaaXaaXaaCysXaaTrpXaa 1234567891011121314 and a second amino acid sequence having the following sequence TrpCysCysXaaCys(SEQ ID NO: 80) 12345 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    摘要翻译: 本发明涉及酶制剂,其基本上由具有纤维素分解活性的酶组成,并且包含具有以下序列的第一氨基酸序列和具有以下序列的第二个氨基酸序列,其中在第一序列的3位是氨基酸 是Trp,Tyr或Phe; 在第一序列的位置4处,氨基酸是Trp,Tyr或Phe; 在第一序列的位置8,氨基酸是Arg,Lys或His; 在第一序列的第9,10,12和14位,第2个序列的第4位,氨基酸是20个天然存在的氨基酸残基中的任一个,条件是在第一个氨基酸序列 ,(i)当第12位的氨基残基为Ser时,第14位的氨基酸残基不为Ser,(ii)当第12位的氨基残基为Gly时,第14位的氨基酸残基为 Ala在工业应用中表现良好,例如洗衣组合物,用于生物抛光新制造的纺织品,用于提供纤维素织物或衣服的磨损外观以及纸浆处理。 此外,本发明涉及编码这些酶的DNA构建体,提供编码这种酶的基因的方法,生产酶的方法,含有这些酶的酶制剂,以及这些酶用于许多工业应用的用途。

    Method of producing a polarizable electrode
    32.
    发明授权
    Method of producing a polarizable electrode 失效
    可极化电极的制造方法

    公开(公告)号:US06702963B2

    公开(公告)日:2004-03-09

    申请号:US09927533

    申请日:2001-08-13

    IPC分类号: C01B3102

    CPC分类号: H01G9/155 H01M4/96 Y02E60/13

    摘要: A polarizable electrode (1) includes an activated carbon/carbon composite consisting essentially of an activated carbon component and a carbon component. The activated carbon component includes activated carbon powder or fiber. The carbon component is produced by carbonizing a thermosetting resin. The composite has a density within a range between 0.70 and 0.85 g/cm3, both inclusive, and a thickness within another range between 0.7 and 3.0 cm, both inclusive.

    摘要翻译: 可极化电极(1)包括主要由活性炭组分和碳组分组成的活性炭/碳复合材料。 活性炭成分包括活性炭粉末或纤维。 碳组分通过将热固性树脂碳化来制备。 复合材料的密度在0.70和0.85g / cm 3之间,包括端值在内,0.7〜3.0cm的范围内的厚度。

    Endoglucanases
    33.
    发明授权
    Endoglucanases 有权
    内切葡聚糖酶

    公开(公告)号:US06387690B1

    公开(公告)日:2002-05-14

    申请号:US09229911

    申请日:1999-01-13

    IPC分类号: D06M1600

    摘要: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence consisting of 14 amino acid residues having the following sequence Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14 and a second amino acid sequence consisting of 5 amino acid residues having the following sequence Trp Cys Cys Xaa Cys 1   2   3   4   5 wherein, in position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 8 of the first sequence, the amino acid is Arg, Lys or His; in position 9, 10, 12 and 14, respectively, of the first sequence, and in position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue in position 12 is Ser, then the amino acid residue in position 14 is not Ser, and (ii) when the amino residue in position 12 is Gly, then the amino acid residue in position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

    摘要翻译: 本发明涉及酶制剂,其基本上由具有纤维素分解活性的酶组成,并且包含由具有以下序列的14个氨基酸残基组成的第一氨基酸序列,以及由具有以下序列的5个氨基酸残基组成的第二个氨基酸序列,其中, 第一序列的第3位,氨基酸为Trp,Tyr或Phe; 在第一序列的位置4,氨基酸是Trp,Tyr或Phe; 在第一序列的位置8,氨基酸是Arg,Lys或His; 分别位于第一序列的第9,10,12和14位,在第二序列的位置4中,氨基酸是20个天然存在的氨基酸残基中的任一个,条件是在第一个氨基酸序列 ,(i)当位置12的氨基残基为Ser时,14位的氨基酸残基不为Ser,(ii)当位置12的氨基残基为Gly时,位置14的氨基酸残基为 Ala在工业应用中表现非常好,例如洗衣组合物,用于生物抛光新制造的纺织品,用于提供纤维素织物或服装的磨损外观以及纸浆处理。 此外,本发明涉及编码这些酶的DNA构建体,提供编码这种酶的基因的方法,生产酶的方法,含有这些酶的酶制剂,以及这些酶用于许多工业应用的用途。

    POLYNUCLEOTIDES HAVING LEADER SEQUENCE FUNCTION
    35.
    发明申请
    POLYNUCLEOTIDES HAVING LEADER SEQUENCE FUNCTION 有权
    具有领导序列功能的多核苷酸

    公开(公告)号:US20130095525A1

    公开(公告)日:2013-04-18

    申请号:US13704856

    申请日:2011-06-23

    IPC分类号: C12N15/80

    CPC分类号: C12N15/80 C07K14/195

    摘要: The present invention relates to isolated polynucleotides having leader sequence function. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods using the polynucleotides for production of polypeptides.

    摘要翻译: 本发明涉及具有前导序列功能的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体,载体和宿主细胞以及使用多核苷酸产生多肽的方法。

    Lipoxygenase
    37.
    发明申请
    Lipoxygenase 失效
    脂氧合酶

    公开(公告)号:US20110183865A1

    公开(公告)日:2011-07-28

    申请号:US13076803

    申请日:2011-03-31

    摘要: The inventors have found a novel fungal lipoxygenase from Manaporthe salvinii and determined its sequence. They have sequenced the gene and cloned it into E. coli and deposited the clone. Oligonucleotides probes based on the sequence information are useful for screening a eukaryotic library to obtain a lipoxygenase. The lipoxygenase is useful in baking and in a detergent.

    摘要翻译: 本发明人已经发现了一种来自Manaporthe salvinii的新型真菌脂氧合酶并确定其序列。 他们对基因进行了测序,并将其克隆到大肠杆菌中并保藏了该克隆。 基于序列信息的寡核苷酸探针可用于筛选真核文库以获得脂氧合酶。 脂氧合酶可用于烘烤和洗涤剂中。

    Pichia Pastoris Das Promoter Variants
    38.
    发明申请
    Pichia Pastoris Das Promoter Variants 审中-公开
    巴斯德毕赤酵母Das启动子变体

    公开(公告)号:US20110129874A1

    公开(公告)日:2011-06-02

    申请号:US13002631

    申请日:2009-07-10

    CPC分类号: C07K14/39 C12N15/815

    摘要: The present invention relates to promoter variants in the form of an isolated polynucleotide comprising: i) a nucleotide sequence consisting of the DAS promoter sequence from Pichia or a functional part thereof, wherein the said DAS promoter is contained in SEQ ID NO: 1; and ii) at least one additional UAS, wherein the said UAS is comprised in SEQ ID NO: 2.

    摘要翻译: 本发明涉及分离多核苷酸形式的启动子变体,其包含:i)由毕赤氏酵母的DAS启动子序列或其功能部分组成的核苷酸序列,其中所述DAS启动子包含在SEQ ID NO:1中; 和ii)至少一种另外的UAS,其中所述UAS包含在SEQ ID NO:2中。