公开(公告)号：US20110204259A1

公开(公告)日：2011-08-25

申请号：US13033299

申请日：2011-02-23

Applicant: Clare E. Rogers ,  Jack T. Ball ,  Collin A. Rich

Inventor： Clare E. Rogers ,  Jack T. Ball ,  Collin A. Rich

IPC: G01J1/58

CPC classification number: G01N15/1429 ,  G01N15/1459

Abstract: A method for detecting fluorochromes in a flow cytometer, including: receiving a sample including particles each tagged with at least one of a first fluorochrome and a second fluorochrome, in which the first and second fluorochromes having distinct spillover coefficients; detecting the particles, including detecting the first and second fluorochromes with a first detector and a second detector; forming a data set for detected particles based on the detection of the first and second fluorochromes; characterizing a detected spillover coefficient for each detected fluorochrome from the data set; and sorting the detected particles into predicted fluorochrome populations based on the detected spillover coefficients. A system for detecting fluorochromes in a flow cytometer, including a flow cell, a fixed gain detection system, and a processor that generates a detected spillover coefficient for each detected particle and sorts the detected particle into predicted fluorochrome populations based on the detected spillover coefficient.

Abstract translation: 一种用于在流式细胞仪中检测荧光染料的方法，包括：接收包含每个标记有第一荧光染料和第二荧光染料中的至少一种的颗粒的样品，其中第一和第二荧光染料具有不同的溢出系数; 检测颗粒，包括用第一检测器和第二检测器检测第一和第二荧光染料; 基于第一和第二荧光色素的检测形成检测到的颗粒的数据集; 表征来自数据集的每个检测到的荧光染料的检测到的溢出系数; 并且基于检测到的溢出系数将检测到的颗粒分选成预测的荧光染色体群体。 一种用于在流式细胞仪中检测荧光染料的系统，包括流动池，固定增益检测系统和处理器，其产生每个检测到的颗粒的检测到的溢出系数，并且基于检测到的溢出系数将检测到的颗粒分解成预测的荧光染色体群。

公开(公告)号：US20110058168A1

公开(公告)日：2011-03-10

申请号：US12887392

申请日：2010-09-21

Applicant: Collin A. Rich ,  Nathaniel C. Bair

Inventor： Collin A. Rich ,  Nathaniel C. Bair

IPC: G01N21/17

CPC classification number: G01N21/53 ,  G01N15/1434 ,  G01N21/532 ,  G01N21/645 ,  G01N2021/4707 ,  G01N2021/4711 ,  G01N2021/4726 ,  G01N2021/6497 ,  Y10T436/12

Abstract: An optical system for a flow cytometer having a flow channel with an interrogation zone and an illumination source that impinges the flow channel in the interrogation zone includes a lens system and a detection system. The lens system preferably includes at least two lens surfaces located on opposite sides of the flow channel and configured to collect and collimate light from the interrogation zone. The detection system, configured to detect light from the lens system, preferably includes first and second detectors, a first filter that passes a first wavelength of light and reflects a second wavelength of light, and a second filter that reflects the first wavelength of light and passes the second wavelength of light, wherein the first and second filters are aligned such that light reflected from the first filter passes into the second detector and light reflected from the second filter passes into the first detector.

Abstract translation: 具有流动通道的流式细胞仪的光学系统具有询问区的流动通道和将询问区中的流动通道照射的照明源包括透镜系统和检测系统。 透镜系统优选地包括位于流动通道的相对侧上的至少两个透镜表面，并且被配置为收集和准直来自询问区的光。 被配置为检测来自透镜系统的光的检测系统优选地包括第一和第二检测器，通过第一波长的光并反射第二波长的光的第一滤光器和反射第一波长的光的第二滤光器，以及 通过第二波长的光，其中第一和第二滤光器对齐，使得从第一滤光片反射的光线进入第二检测器，并且从第二滤光器反射的光线进入第一检测器。

公开(公告)号：US20090260701A1

公开(公告)日：2009-10-22

申请号：US12425830

申请日：2009-04-17

Applicant: Collin A. Rich ,  Nathaniel C. Bair

Inventor： Collin A. Rich ,  Nathaniel C. Bair

IPC: F16L55/04

CPC classification number: F16L55/04 ,  F04B43/12 ,  F15B1/021 ,  F15B1/10 ,  F15B21/008 ,  F15B2201/205 ,  F15B2201/215 ,  F15B2201/3151 ,  G01N15/1404 ,  G01N15/1459 ,  Y10T137/3118

Abstract: A pulsation attenuator for a fluidic system with a fluidic pump. The pulsation attenuator includes a fluidic channel, a first fluidic device adapted to attenuate pulsations, and a second fluidic device adapted to attenuate pulsations. Preferably, the first fluidic device includes a first fluidic resistor and a first fluidic capacitor, and the second fluidic device includes a second fluidic resistor and a second fluidic capacitor. Preferably, the first fluidic resistor and second fluidic resistor are resistive channels. Preferably, the first fluidic capacitor and second fluidic capacitor include a membrane that expands and accumulates fluid and then contracts and reintroduces the accumulated fluid into the fluidic channel.

Abstract translation: 一种具有流体泵的流体系统的脉动衰减器。 脉动衰减器包括流体通道，适于衰减脉动的第一流体装置，以及适于衰减脉动的第二流体装置。 优选地，第一流体装置包括第一流体电阻器和第一流体电容器，并且第二流体装置包括第二流体电阻器和第二流体电容器。 优选地，第一流体电阻器和第二流体电阻器是电阻通道。 优选地，第一流体电容器和第二流体电容器包括膨胀和积聚流体然后收缩并将积聚的流体再引入流体通道的膜。

公开(公告)号：US20090250729A1

公开(公告)日：2009-10-08

申请号：US12420683

申请日：2009-04-08

Applicant: David F. Lemmerhirt ,  Collin A. Rich

Inventor： David F. Lemmerhirt ,  Collin A. Rich

IPC: H01L25/16 ,  H01L21/28

CPC classification number: B06B1/0292 ,  A61B8/00 ,  A61B8/4472

Abstract: The integrated circuit/transducer device of the preferred embodiment includes a substrate, a complementary-metal-oxide-semiconductor (CMOS) circuit that is fabricated on the substrate, and a capacitive micromachined ultrasonic transducer (cMUT) element that is also fabricated on the substrate. The CMOS circuit and cMUT element are fabricated during the same foundry process and are connected. The cMUT includes a lower electrode, an upper electrode, a membrane structure that support the upper electrode, and a cavity between the upper electrode and lower electrode.

Abstract translation: 优选实施例的集成电路/换能器装置包括衬底，制造在衬底上的互补金属氧化物半导体（CMOS）电路，以及还制造在衬底上的电容微加工超声换能器（cMUT）元件 。 CMOS电路和cMUT元件在相同的铸造过程中制造并连接。 cMUT包括下电极，上电极，支撑上电极的膜结构，以及上电极和下电极之间的空腔。

公开(公告)号：US20090174881A1

公开(公告)日：2009-07-09

申请号：US12337517

申请日：2008-12-17

Applicant: Collin A. Rich

Inventor： Collin A. Rich

IPC: G01N1/28

CPC classification number: G01N15/1434 ,  G01N15/1459

Abstract: The optical system of the preferred embodiments includes a first light source that creates a first beam of a first wavelength, a first collimating element that collimates the first beam, a second light source 102 that creates a second beam of a second wavelength, a second collimating element that collimates the second beam, a beam combining element that combines the collimated beams, and a focusing element that focuses the combined collimated beam to a single point.

Abstract translation: 优选实施例的光学系统包括产生第一波长的第一光束的第一光源，准直第一光束的第一准直元件，产生第二波长的第二光束的第二光源102，第二准直 对第二光束进行准直的元件，组合准直光束的光束组合元件以及将组合的准直光束聚焦到单个点的聚焦元件。

公开(公告)号：US20090104075A1

公开(公告)日：2009-04-23

申请号：US11549560

申请日：2006-10-13

Applicant: Collin A. Rich

Inventor： Collin A. Rich

IPC: G01N35/08 ,  G05D7/06 ,  B01L3/00 ,  G05B13/02

CPC classification number: G01N15/1404 ,  G01N2015/1413 ,  G05D7/0682 ,  Y10T436/115831 ,  Y10T436/117497

Abstract: The fluidic system of the preferred embodiment includes a sheath pump to pump sheath fluid from a sheath container into an interrogation zone and a waste pump to pump waste fluid from the interrogation zone into a waste container. The sheath pump and/or the waste pump draw sample fluid from a sample container into the interrogation zone. The fluidic system also includes a controller to adjust the flow rate of the sample fluid from the sample container into the interrogation zone. The fluidic system is preferably incorporated into a flow cytometer with a flow cell that includes the interrogation zone.

Abstract translation: 优选实施例的流体系统包括护套泵，以将护套流体从护套容器泵送到询问区域，以及废物泵将废液从询问区泵送到废物容器中。 护套泵和/或废水泵将样品流体从样品容器中抽入询问区。 流体系统还包括控制器，用于调节样品流体从样品容器进入询问区域的流速。 流体系统优选地结合到具有包括询问区的流动池的流式细胞仪中。

公开(公告)号：US20080055595A1

公开(公告)日：2008-03-06

申请号：US11848229

申请日：2007-08-30

Applicant: David C. Olson ,  Collin A. Rich

Inventor： David C. Olson ,  Collin A. Rich

IPC: G01J3/30 ,  G01J3/443

CPC classification number: G01J3/4338 ,  G01N21/645 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441

Abstract: The invention includes a system and a method for capturing multi source excitations from a single location on a flow channel. The system preferably includes a light subsystem that emits light onto a single location on a flow channel, a detector subsystem to detect light emitted from the single location on the flow channel, and a processor to separate the detected light. The method preferably includes emitting light onto a single location on a flow channel, detecting light emitted from the single location on the flow channel, and separating the detected light.

Abstract translation: 本发明包括一种用于从流通道上的单个位置捕获多源兴奋的系统和方法。 该系统优选地包括将光发射到流动通道上的单个位置的光子系统，用于检测从流动通道上的单个位置发射的光的检测器子系统以及用于分离检测到的光的处理器。 该方法优选地包括将光发射到流动通道上的单个位置，检测从流动通道上的单个位置发射的光并分离检测到的光。

公开(公告)号：US06824521B2

公开(公告)日：2004-11-30

申请号：US10054125

申请日：2002-01-22

Applicant: Collin A. Rich ,  F. Randall Grimes

Inventor： Collin A. Rich ,  F. Randall Grimes

IPC: A61B500

CPC classification number: A61B5/6852 ,  A61B5/0031 ,  A61B5/02028 ,  A61B5/0215 ,  A61B5/027 ,  A61B5/0295 ,  A61B5/14532 ,  A61B5/14539 ,  A61B5/14546 ,  A61B5/1473 ,  A61B5/4839 ,  A61B5/6862 ,  A61B5/6876 ,  A61B5/6882 ,  A61B2562/028

Abstract: A microminiature sensing module and a sensing catheter system are provided. The module includes a sensor and signal conditioning circuit mounted on a substrate having electrical contacts positioned to facilitate connection of the module to an electrical bus, such as a dual lead electrical bus. The catheter system incorporates a dual lead electrical bus and one or more sensor modules. Methods of fabricating sensing catheter systems are also provided.

Abstract translation: 提供微型感测模块和感测导管系统。 该模块包括安装在基板上的传感器和信号调节电路，该基板具有定位成便于将模块连接到电气总线（例如双引线电气总线）的电触点。 导管系统包括双引线电气总线和一个或多个传感器模块。 还提供了制造感测导管系统的方法。

公开(公告)号：US09280635B2

公开(公告)日：2016-03-08

申请号：US13820968

申请日：2011-10-25

Applicant: Collin A. Rich

Inventor： Collin A. Rich

IPC: G01N33/48 ,  G01N33/50 ,  G01N31/00 ,  G06F19/10 ,  G06F19/26 ,  G01N15/14 ,  G06F19/12 ,  G06M11/02

CPC classification number: G01N15/1434 ,  G01J3/0208 ,  G01J3/0229 ,  G01J3/18 ,  G01J3/36 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1429 ,  G06F19/10 ,  G06F19/12 ,  G06F19/26 ,  G06M11/02

Abstract: Systems in a flow cytometer having an interrogation zone and illumination impinging the interrogation zone include: a lens subsystem including a collimating element that collimates light from the interrogation zone, a light dispersion element that disperses collimated light into a light spectrum, and a focusing lens that focuses the light spectrum onto an array of adjacent detection points; a detector array, including semiconductor detector devices, that collectively detects a full spectral range of input light signals, in which each detector device detects a subset spectral range of the full spectral range of light signals; and a user interface that enables a user to create a set of virtual detector channels by grouping detectors in the detector array, such that each virtual detector channel corresponds to a detector group and has a virtual detector channel range including the sum of subset spectral ranges of the detectors in the corresponding detector group.

Abstract translation: 具有询问区和照射询问区的照明的流式细胞仪中的系统包括：透镜子系统，包括准直来自询问区的光的准直元件，将准直光分散到光谱中的光分散元件和聚焦透镜， 将光谱聚焦到相邻检测点的阵列上; 检测器阵列，包括半导体检测器装置，其共同地检测输入光信号的全光谱范围，其中每个检测器装置检测光信号的全光谱范围的子集光谱范围; 以及用户界面，其使得用户能够通过对检测器阵列中的检测器进行分组来创建一组虚拟检测器通道，使得每个虚拟检测器通道对应于检测器组并且具有虚拟检测器通道范围，该虚拟检测器通道范围包括： 相应检测器组中的检测器。

公开(公告)号：US08445286B2

公开(公告)日：2013-05-21

申请号：US12514052

申请日：2007-11-07

Applicant: Nathaniel C. Bair ,  Collin A. Rich ,  Mark Robert Eadie ,  Rebecca Ann Lehrmann

Inventor： Nathaniel C. Bair ,  Collin A. Rich ,  Mark Robert Eadie ,  Rebecca Ann Lehrmann

IPC: G01N33/48 ,  G01N35/08 ,  G01N21/00 ,  G01N33/00 ,  G01N35/00

CPC classification number: G01N15/1404 ,  G01N2015/1413 ,  Y10T29/49826 ,  Y10T137/0352 ,  Y10T137/0379 ,  Y10T137/0419 ,  Y10T436/115831 ,  Y10T436/117497

Abstract: The flow cytometer system of the preferred embodiment includes a flow cell body that functions to contain, protect, and align the components of the flow cytometer system; a flow channel, coupled to the flow cell body, that functions to conduct and focus sample fluid through an interrogation zone; and a sample injection probe, removably coupled to the flow cell body, that functions to provide a uniform flow of sample fluid to the flow channel. The flow cytometer system is preferably designed for the flow cytometer field. The flow cytometer system, however, may be alternatively used in any suitable environment and for any suitable reason.

Abstract translation: 优选实施例的流式细胞仪系统包括流动池体，其用于容纳，保护和对准流式细胞仪系统的组件; 耦合到流动池主体的流动通道，其用于将样品流体引导和聚焦通过询问区; 以及可移除地耦合到流动池主体的样​​品注入探针，其用于提供样品流体到流动通道的均匀流动。 流式细胞仪系统优选设计用于流式细胞仪领域。 然而，流式细胞仪系统可以替代地在任何合适的环境中用于任何合适的原因。