公开(公告)号：US20250128256A1

公开(公告)日：2025-04-24

申请号：US18832923

申请日：2023-01-24

Applicant: President and Fellows of Harvard College

Inventor： Mohamed Yafia Okba Salem ,  Donald E. Ingber

IPC: B01L3/00

Abstract: Devices and methods related to microfluidic devices with autonomous directional valves are generally described.

公开(公告)号：US12065634B2

公开(公告)日：2024-08-20

申请号：US17871054

申请日：2022-07-22

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Daniel Levner ,  Guy Thompson, II ,  Jose Fernandez-Alcon ,  Christopher David Hinojosa

IPC: C12M1/00 ,  B01L3/00 ,  B01L3/02 ,  C12M1/12 ,  C12M1/36 ,  C12M3/00 ,  C12M3/06

CPC classification number: C12M29/10 ,  B01L3/502715 ,  C12M21/08 ,  C12M23/16 ,  C12M23/38 ,  C12M23/58 ,  C12M29/00 ,  C12M29/20 ,  C12M37/04 ,  C12M41/48 ,  B01L3/0293 ,  B01L2200/027 ,  B01L2300/0816

Abstract: Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

公开(公告)号：US20240248077A1

公开(公告)日：2024-07-25

申请号：US18436701

申请日：2024-02-08

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Kambez Hajipouran Benam ,  Remi Villenave ,  Geraldine A. Hamilton ,  Bryan Hassell ,  Christopher D. Hinojosa ,  Carolina Lucchesi

IPC: G01N33/50 ,  C12M1/12 ,  C12M1/34 ,  C12M1/42 ,  C12M3/00 ,  C12M3/06

CPC classification number: G01N33/5044 ,  C12M21/08 ,  C12M23/16 ,  C12M25/02 ,  C12M35/04 ,  C12M41/46 ,  G01N2800/122

Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.

公开(公告)号：US20240140836A1

公开(公告)日：2024-05-02

申请号：US17768734

申请日：2020-09-30

Applicant: President and Fellows of Harvard College

Inventor： Elizabeth Calamari ,  Richard Novak ,  Manuel Ramses Martinez Flores ,  Alexandre L.M. Dinis ,  Robert Cunningham ,  Olivier Henry ,  Donald E. Ingber ,  Jeremy J. Papadopoulos

IPC: C02F1/48

CPC classification number: C02F1/48 ,  C02F2301/028 ,  C02F2303/04

Abstract: Microfluidic devices for sterilizing fluids using pulsed electric fields are disclosed. In some embodiments, a device may include first and second electrode layers and a spacer layer positioned between the electrode layers. The spacer layer may define one or more fluid channels extending between the electrode layers. A power supply may be coupled to the electrode layers and configured to supply voltage pulses to the electrode layers to generate electric field pulses within the fluid channels. In some embodiments, the electrode layers may be textured such that the electric fields generated in the fluid channels are non-uniform.

公开(公告)号：US20240084235A1

公开(公告)日：2024-03-14

申请号：US18470404

申请日：2023-09-19

Applicant: President and Fellows of Harvard College

Inventor： Jose Fernandez-Alcon ,  Norman Wen ,  Richard Novak ,  Donald E. Ingber ,  Geraldine A. Hamilton ,  Christopher Hinojosa ,  Karel Domansky ,  Daniel Levner ,  Guy Thompson, II ,  Kambez Hajipouran Benam ,  Remi Villenave ,  Thomas Umundum ,  Alfred Paris ,  Georg Bauer

IPC: C12M3/06 ,  B01D61/18 ,  B01D63/08 ,  B01D67/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M1/42 ,  C12M3/00

CPC classification number: C12M23/16 ,  B01D61/18 ,  B01D63/081 ,  B01D67/0023 ,  B01L3/5027 ,  B01L3/502753 ,  C12M21/08 ,  C12M23/26 ,  C12M25/02 ,  C12M35/04 ,  C12M41/00 ,  C12M41/46 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0887 ,  B01L2400/0487

Abstract: An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.

公开(公告)号：US11884938B2

公开(公告)日：2024-01-30

申请号：US16688215

申请日：2019-11-19

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Hyun Jung Kim

IPC: C12M3/00 ,  B01L3/00 ,  C12N5/071 ,  C12M1/12 ,  C12N1/20 ,  C12M1/42 ,  C12N1/14 ,  C12M1/24 ,  C12M1/00 ,  C12M1/06 ,  C12M1/34 ,  C12N5/074

CPC classification number: C12N5/0697 ,  C12M21/08 ,  C12M23/08 ,  C12M23/20 ,  C12M23/26 ,  C12M23/34 ,  C12M23/38 ,  C12M25/02 ,  C12M27/02 ,  C12M29/04 ,  C12M29/06 ,  C12M35/04 ,  C12M35/08 ,  C12M41/12 ,  C12M41/26 ,  C12M41/40 ,  C12M41/44 ,  C12N1/14 ,  C12N1/20 ,  C12N5/068 ,  C12N5/0679 ,  C12N5/0696

Abstract: The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.

公开(公告)号：US11845920B2

公开(公告)日：2023-12-19

申请号：US16496997

申请日：2018-03-26

Applicant: President and Fellows of Harvard College ,  The General Hospital Corporation

Inventor： David Benson Chou ,  Liliana S. Teixeira Moreira Leijten ,  Arianna Rech ,  Richard Novak ,  Donald E. Ingber ,  Yuka Milton ,  Viktoras Frismantas ,  Oren Levy

IPC: C12M3/06 ,  C12N5/077 ,  C12M1/00 ,  C12M1/12 ,  B01L3/00 ,  G01N33/50

CPC classification number: C12M23/16 ,  B01L3/502715 ,  C12M25/02 ,  C12M29/10 ,  C12N5/0669 ,  G01N33/5008 ,  B01L2300/12 ,  C12N2501/40 ,  C12N2501/999 ,  C12N2502/28 ,  C12N2533/54 ,  C12N2533/56 ,  C12N2533/90

Abstract: The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.

公开(公告)号：US20230159872A1

公开(公告)日：2023-05-25

申请号：US18056035

申请日：2022-11-16

Applicant: President and Fellows of Harvard College

Inventor： Antonio Varone ,  Norman Wen ,  Daniel Levner ,  Richard Novak ,  Lori McPartlin ,  Donald E. Ingber ,  Youngjae Choe ,  Lian Leng ,  Justin K. Nguyen

IPC: C12M3/06 ,  C12M3/00 ,  C12M1/12 ,  C12N5/071 ,  C12N5/077

CPC classification number: C12M23/16 ,  C12M21/08 ,  C12M25/02 ,  C12M25/14 ,  C12N5/0629 ,  C12N5/0656 ,  C12N5/0679 ,  C12N5/069 ,  B01L3/5027

Abstract: A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

公开(公告)号：US11499131B2

公开(公告)日：2022-11-15

申请号：US16593646

申请日：2019-10-04

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Daniel Levner ,  Guy Thompson, II ,  Jose Fernandez-Alcon ,  Christopher David Hinojosa

IPC: C12M1/00 ,  C12M3/06 ,  B01L3/00 ,  C12M3/00 ,  C12M1/12 ,  C12M1/36 ,  B01L3/02

Abstract: Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

公开(公告)号：US11406975B2

公开(公告)日：2022-08-09

申请号：US16318591

申请日：2017-07-18

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Girija Goyal ,  Donald E. Ingber

IPC: B01L3/00 ,  C12N5/0781 ,  C12N5/0783 ,  A61K35/28 ,  A61P35/00 ,  A61K35/17 ,  C12M3/06 ,  A61P37/04 ,  C12M3/00 ,  A61P7/00 ,  C12N5/00 ,  C12N5/0786 ,  C12N5/078 ,  C12N5/071 ,  C12N5/09 ,  G01N33/50

Abstract: An organ-on-a-chip microfluidic device is disclosed that mimics a human lymph node and/or human lymphoid tissue. The device can include cells from human blood and lymphatic tissue, include an extracellular matrix for the development of immune system components, and provide for the perfusion of fluids and solids resembling blood and lymphatic fluid within micrometer sized channels.