公开(公告)号：US20090148915A1

公开(公告)日：2009-06-11

申请号：US11877726

申请日：2007-10-24

申请人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Yuta Nakai ,  Tomoko Suzuki ,  Mika Moriya ,  Yuichiro Tsuji ,  Takuji Ueda

发明人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Yuta Nakai ,  Tomoko Suzuki ,  Mika Moriya ,  Yuichiro Tsuji ,  Takuji Ueda

IPC分类号： C12P13/08

CPC分类号： C12N9/0006 ,  C12P13/08

摘要： A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.

摘要翻译： 属于埃希氏菌属的细菌，其具有产生L-赖氨酸或L-苏氨酸的能力，并且其被修饰以使得苹果酸在细胞中不起作用;以及L-赖氨酸或L-苏氨酸的制备方法， 包括在培养基中培养细菌以产生并引起L-赖氨酸或L-苏氨酸的积累，并从培养基中收集L-赖氨酸或L-苏氨酸。

公开(公告)号：US20090068712A1

公开(公告)日：2009-03-12

申请号：US12202476

申请日：2008-09-02

申请人： Masaru Terashita ,  Yoshihiro Usuda ,  Kazuhiko Matsui

发明人： Masaru Terashita ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12P13/22 ,  C12N1/00 ,  C12N1/21 ,  C12P13/08 ,  C12P13/06

CPC分类号： C12N9/0008 ,  C12P13/06 ,  C12P13/08 ,  C12P13/222 ,  C12P13/227

摘要： A microorganism is provided which has an ability to produce an L-amino acid such as L-lysine, L-tryptophan, L-phenylalanine, L-valine, L-leucine, L-isoleucine and L-serine, and has been modified to increase the activity of pyruvate synthase or pyruvate:NADP+ oxidoreductase. This microorganism is cultured in a medium containing ethanol or an aliphatic acid as the carbon source to produce and accumulate the L-amino acid in the medium or cells, and the L-amino acid is collected from the medium or the cells.

摘要翻译： 提供了具有产生L-赖氨酸，L-色氨酸，L-苯丙氨酸，L-缬氨酸，L-亮氨酸，L-异亮氨酸，L-丝氨酸等L-氨基酸的能力的微生物， 增加丙酮酸合酶或丙酮酸：NADP +氧化还原酶的活性。 将该微生物在含有乙醇或脂肪酸作为碳源的培养基中培养，以在培养基或细胞中产生并积累L-氨基酸，从培养基或细胞收集L-氨基酸。

公开(公告)号：US20060154344A1

公开(公告)日：2006-07-13

申请号：US11275437

申请日：2006-01-03

申请人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Yuta Nakai ,  Tomoko Suzuki ,  Mika Moriya ,  Yuichiro Tsuji ,  Takuji Ueda

发明人： Stephen Van Dien ,  Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui ,  Yuta Nakai ,  Tomoko Suzuki ,  Mika Moriya ,  Yuichiro Tsuji ,  Takuji Ueda

IPC分类号： C12P13/08 ,  C12N1/21 ,  C12N15/74

CPC分类号： C12N9/0006 ,  C12P13/08

摘要： A bacterium belonging to the genus Escherichia which has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.

摘要翻译： 属于埃希氏菌属的细菌，其具有产生L-赖氨酸或L-苏氨酸的能力，并且其被修饰以使得苹果酸在细胞中不起作用;以及L-赖氨酸或L-苏氨酸的制备方法， 包括在培养基中培养细菌以产生并引起L-赖氨酸或L-苏氨酸的积累，并从培养基中收集L-赖氨酸或L-苏氨酸。

公开(公告)号：US20050175982A1

公开(公告)日：2005-08-11

申请号：US11048923

申请日：2005-02-03

申请人： Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui

发明人： Shintaro Iwatani ,  Yoshihiro Usuda ,  Kazuhiko Matsui

IPC分类号： C12Q1/00 ,  G01N33/50 ,  G06F19/12 ,  G01N33/48 ,  G06F19/00

CPC分类号： G01N33/5091 ,  C12Q1/00 ,  G06F19/12

摘要： A method for intracellular metabolic flux analysis comprising (a) culturing cells in a medium not containing any isotope-labeled substrate to a target phase of the metabolic flux analysis, (b) adding an isotope-labeled substrate to the medium, and continuing culture and collecting samples from the medium in time course, (c) measuring isotope distribution in an intracellular metabolite contained in the samples collected in time course, (d) performing a regression analysis for measured data and calculating an isotope distribution ratio in a steady state, and (e) analyzing a metabolic flux of the cultured cells by using the calculated isotope distribution ratio.

摘要翻译： 一种用于细胞内代谢通量分析的方法，包括（a）在不含任何同位素标记的底物的培养基中将细胞培养至代谢通量分析的目标阶段，（b）将同位素标记的底物加入到培养基中， 在时间过程中从培养基中收集样品，（c）测量在时间过程中收集的样品中所含的细胞内代谢物中的同位素分布，（d）对稳定状态进行测量数据和计算同位素分布比的回归分析，以及 （e）使用计算出的同位素分布比分析培养细胞的代谢通量。

公开(公告)号：US08129151B2

公开(公告)日：2012-03-06

申请号：US11624080

申请日：2007-01-17

申请人： Mika Moriya ,  Hiroshi Izui ,  Eiji Ono ,  Kazuhiko Matsui ,  Hisao Ito ,  Yoshihiko Hara

发明人： Mika Moriya ,  Hiroshi Izui ,  Eiji Ono ,  Kazuhiko Matsui ,  Hisao Ito ,  Yoshihiko Hara

IPC分类号： C12N1/00 ,  C12N1/12 ,  C12N1/20 ,  C12P1/04 ,  C12P13/04 ,  C12P13/14

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01 ,  C12R1/425 ,  Y10S435/822 ,  Y10S435/88

摘要： L-Glutamic acid is produced by culturing in a liquid culture medium a microorganism belonging to the genus Pantoea or Serratia and having an ability to produce L-glutamic acid, which increases in an activity of enzyme catalyzing a reaction for L-glutamic acid biosynthesis or which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, and collecting produced L-glutamic acid from the culture medium.

摘要翻译： L-谷氨酸通过在液体培养基中培养属于泛酸属或沙雷氏菌属的具有产生L-谷氨酸能力的微生物产生，其增加了催化L-谷氨酸生物合成反应的酶的活性，或 其催化从L-谷氨酸生物合成途径分支的反应的酶的活性降低或缺乏，并产生除L-谷氨酸以外的化合物，并从培养基中收集L-谷氨酸。

公开(公告)号：US07608437B2

公开(公告)日：2009-10-27

申请号：US11218433

申请日：2005-09-06

申请人： Yoko Asakura ,  Jun Nakamura ,  Sohei Kanno ,  Mikiko Suga ,  Eiichiro Kimura ,  Hisao Ito ,  Kazuhiko Matsui ,  Tsuyoshi Ohsumi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

发明人： Yoko Asakura ,  Jun Nakamura ,  Sohei Kanno ,  Mikiko Suga ,  Eiichiro Kimura ,  Hisao Ito ,  Kazuhiko Matsui ,  Tsuyoshi Ohsumi ,  Tsuyoshi Nakamatsu ,  Osamu Kurahashi

IPC分类号： C12P13/14 ,  C12Q1/48 ,  C12Q1/32 ,  C12Q1/68 ,  C12N9/02 ,  C12N9/10 ,  C12N1/21 ,  C12P21/00 ,  C12N15/00 ,  C07K14/00 ,  C07H21/00

CPC分类号： C12P13/14 ,  C12N15/67

摘要： A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.

摘要翻译： 具有改进的氨基酸或核酸生产力的棒状杆菌细菌的方法包括以下步骤：将氨基酸或核酸 - 生物合成基因的启动子序列中的突变引入棒状细菌的染色体上，使其接近 共有序列或通过基因重组在棒状细菌的染色体上引入氨基酸或核酸 - 生物合成基因的启动子序列的变化，使得其接近共有序列，获得棒状杆菌型氨基酸的突变体或 产生核酸的微生物，培养突变体并选择能够大量产生所需氨基酸或核酸的突变体。 该方法允许本领域技术人员通过重组或突变来构建能够富集或控制预期基因的表达而不使用质粒并以高产率促进氨基酸产生的突变体。

公开(公告)号：US07090998B2

公开(公告)日：2006-08-15

申请号：US10613990

申请日：2003-07-08

申请人： Yukiko Ishikawa ,  Akira Imaizumi ,  Kazuhiko Matsui ,  Hiroyuki Kojima

发明人： Yukiko Ishikawa ,  Akira Imaizumi ,  Kazuhiko Matsui ,  Hiroyuki Kojima

IPC分类号： C12P1/04 ,  C12N1/20

CPC分类号： C07K14/245

摘要： In a method for producing a target substance utilizing a microorganism, which comprises culturing a γ-proteobacterium in a medium to produce and accumulate the target substance in the medium or cells and collecting the target substance, there is used a strain in which the ArcA protein does not normally function in the cell by means of, for example, disruption of the arcA gene on the chromosome.

摘要翻译： 在利用微生物生产目标物质的方法中，其包括在培养基中培养γ-谷氨酸杆菌，以在培养基或细胞中产生并积累目标物质并收集目标物质，使用其中的ArcA蛋白 通常不会通过例如染色体上arcA基因的破坏在细胞中起作用。

公开(公告)号：US07015010B1

公开(公告)日：2006-03-21

申请号：US09641892

申请日：2000-08-18

申请人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

发明人： Hiroshi Izui ,  Mika Moriya ,  Seiko Hirano ,  Yoshihiko Hara ,  Hisao Ito ,  Kazuhiko Matsui

IPC分类号： C12Q1/04

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01

摘要： A microorganism which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH; and a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

摘要翻译： 在含有饱和浓度的L-谷氨酸和碳源的液体培养基中，能够在特定pH下代谢碳源的微生物，并且具有以超过相应于饱和浓度的量累积L-谷氨酸的能力 在液体介质中的pH值; 以及通过发酵生产L-谷氨酸的方法，其包括在将pH调节至L-谷氨酸沉淀的pH的液体培养基中培养微生物以产生并积累L-谷氨酸并将L-谷氨酸沉淀， 培养基中的谷氨酸。

公开(公告)号：US20090263874A1

公开(公告)日：2009-10-22

申请号：US11624080

申请日：2007-01-17

申请人： Mika Moriya ,  Hiroshi Izui ,  Eiji Ono ,  Kazuhiko Matsui ,  Hisao Ito ,  Yoshihiko Hara

发明人： Mika Moriya ,  Hiroshi Izui ,  Eiji Ono ,  Kazuhiko Matsui ,  Hisao Ito ,  Yoshihiko Hara

IPC分类号： C12P13/14

CPC分类号： C12N9/0016 ,  C12N9/88 ,  C12P13/14 ,  C12R1/01 ,  C12R1/425 ,  Y10S435/822 ,  Y10S435/88

摘要： L-Glutamic acid is produced by culturing in a liquid culture medium a microorganism belonging to the genus Enterobacter or Serratia and having an ability to produce L-glutamic acid, which increases in an activity of enzyme catalyzing a reaction for L-glutamic acid biosynthesis or which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, and collecting produced L-glutamic acid from the culture medium.

摘要翻译： L-谷氨酸通过在液体培养基中培养属于肠杆菌属或沙雷氏菌属的具有生产L-谷氨酸能力的微生物产生，其增加了催化L-谷氨酸生物合成的反应的酶的活性，或 其催化从L-谷氨酸生物合成途径分支的反应的酶的活性降低或缺乏，并产生除L-谷氨酸以外的化合物，并从培养基中收集L-谷氨酸。

公开(公告)号：US07357879B2

公开(公告)日：2008-04-15

申请号：US10560421

申请日：2005-03-01

申请人： Tsunehisa Takahashi ,  Shigeki Sawa ,  Kazuhiko Matsui

发明人： Tsunehisa Takahashi ,  Shigeki Sawa ,  Kazuhiko Matsui

IPC分类号： B44C1/22 ,  C03C15/00 ,  C03C25/68 ,  C23F1/00 ,  C25F3/00

CPC分类号： C23F1/18 ,  C09K13/00 ,  C23F1/02 ,  C23F1/34 ,  H01L21/32134 ,  H05K3/064 ,  H05K3/067 ,  H05K3/383 ,  H05K2203/0597 ,  H05K2203/124

摘要： There is provided an etching solution comprised of a cupric chloride solution and a high-concentration triazole type compound added to the cupric chloride solution and capable of forming an etching-inhibiting coating. In a process of forming a circuit pattern by etching with the etching solution, an etching-inhibiting coating is selected formed on parts of a copper foil laid under the edge of an etching resist to effectively inhibit horizontal side-etching of the copper foil from the edge of the etching resist. Also, nonuniform irregularities formed on the side wall of the circuit pattern by the etching improves the adhesion between the circuit pattern and an insulating resin layer covering the circuit pattern.

摘要翻译： 提供了由氯化铜溶液和加入到氯化铜溶液中的能够形成蚀刻抑制涂层的高浓度三唑型化合物组成的蚀刻溶液。 在通过用蚀刻溶液蚀刻形成电路图案的过程中，在布置在抗蚀剂边缘的铜箔的部分上形成蚀刻抑制涂层，以有效地抑制铜箔的水平侧蚀 边缘的抗蚀剂。 此外，通过蚀刻形成在电路图案的侧壁上的不均匀的凹凸改善了电路图案和覆盖电路图案的绝缘树脂层之间的粘附。