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236 条记录 (耗时 0.026 秒)

    Device containing non-covalently bound biomolecules on solid support
    32.
    发明申请
    Device containing non-covalently bound biomolecules on solid support 审中-公开
    在固体支持物上含有非共价结合的生物分子的装置

    公开(公告)号：US20060093518A1

    公开(公告)日：2006-05-04

    申请号：US11259777

    申请日：2005-10-27

    申请人： Ashok Shukla ,  Mukta Shukla

    发明人： Ashok Shukla ,  Mukta Shukla

    IPC分类号： G01N31/22

    CPC分类号： B01J20/345 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3274 ,  B01J20/3425 ,  B01J20/3475 ,  B01J2220/52 ,  B01J2220/64 ,  B01L3/0275 ,  B01L3/5085 ,  B01L2300/0681 ,  B01L2300/0829 ,  G01N30/6065 ,  G01N2030/085

    摘要： The present invention relates to a novel device for transport of chemicals and biochemicals in small amounts such as in microgram range. For example, a pipette tip, or a film containing chromatographic media, wherein the media contains an enzyme for the enzymic action. The enzyme is adsorbed on the surface of the chromatographic media and is non-covalently bound. These enzyme containing tips will be used for the transport of very small amounts of enzyme to the sample, where the biochemical reaction takes place. They will also be used in a buffer where the enzyme will be kept adsorbed on the chromatographic media and will not dissociate from chromatographic media and the substrate will react with the enzyme on the surface of the media. Furthermore, the presence of chromatographic media can be used simultaneously for the purification of the reaction product from the buffer components.

    摘要翻译： 本发明涉及一种用于少量输送化学品和生物化学品的新型装置，例如微克范围。 例如，移液管尖端或含有色谱介质的膜，其中介质含有用于酶作用的酶。 该酶被吸附在色谱介质的表面上并且非共价结合。 这些含酶的尖端将用于将非常少量的酶运输到生物化学反应发生的样品中。 它们也将用于缓冲液中，其中酶将被吸附在色谱介质上，不会与色谱介质分离，底物将与介质表面上的酶反应。 此外，色谱介质的存在可以同时用于从缓冲组分纯化反应产物。

    Foamed material filled with inner material
    39.
    发明授权
    Foamed material filled with inner material 失效
    发泡材料充满内部材料

    公开(公告)号：US06635174B1

    公开(公告)日：2003-10-21

    申请号：US09979441

    申请日：2002-02-12

    申请人： Hans Berg ,  Mats Carlsson

    发明人： Hans Berg ,  Mats Carlsson

    IPC分类号： B01D1508

    CPC分类号： B01J20/286 ,  B01J20/26 ,  B01J20/28004 ,  B01J20/28014 ,  B01J20/28016 ,  B01J20/28023 ,  B01J20/28026 ,  B01J20/28042 ,  B01J20/28045 ,  B01J20/28085 ,  B01J20/28092 ,  B01J20/28095 ,  B01J20/28097 ,  B01J20/285 ,  B01J2220/52

    摘要： A matrix including: a) a polymeric base matrix including macropores (pore system 1) and b) an interior material, possibly porous (pore system 2), retained within the macropores. The matrix is characterized in that there is a continuous free volume between the interior material and the pore walls of the macropores. A method for manufacturing a matrix including a base matrix having macropores in which an interior material is located. The method is characterized in including the steps: (I) providing a base matrix having macropores; (ii) filling the macropores with a soluble form of the interior material; (iii) transforming the insoluble from to an insoluble form; (iv) shrinking the insoluble form; and (v) irreversibly stabilizing the material in its shrunken form. The matrix can be used in separation methods, cell culturing, solid phase synthesis of organic molecules, and in catalytic reactions (such as enzyme reactions) and other uses in which porous support matrices are used.

    摘要翻译： 一种基体，包括：a）包含大孔（孔体系1）的聚合物基质基质和b）保留在大孔内的可能是多孔（孔系统2）的内部材料。 该基质的特征在于，在大孔的内部材料和孔壁之间存在连续的自由体积。 一种制造基体的方法，其包括具有内部材料所在的大孔的基体基体。 该方法的特征在于包括以下步骤：（I）提供具有大孔的基质矩阵; （ii）用可溶形式的内部材料填充大孔; （iii）将不溶物转化为不溶形式; （iv）收缩不溶形式; 和（v）不可逆地稳定其收缩形式的材料。 该基质可用于分离方法，细胞培养，有机分子的固相合成，以及催化反应（如酶反应）以及使用多孔载体基质的其他用途。