公开(公告)号：US20040235159A1

公开(公告)日：2004-11-25

申请号：US10873922

申请日：2004-06-21

发明人： Ramkumar Mandalam ,  Chunhui Xu

IPC分类号： C12N005/08

CPC分类号： C12N15/1096 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0075 ,  C12N5/0603 ,  C12N5/0606 ,  C12N5/0607 ,  C12N5/0619 ,  C12N5/0622 ,  C12N5/0662 ,  C12N5/0671 ,  C12N5/0672 ,  C12N5/0678 ,  C12N5/068 ,  C12N5/0692 ,  C12N5/0693 ,  C12N5/0696 ,  C12N11/04 ,  C12N15/1034 ,  C12N2500/25 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2500/99 ,  C12N2501/065 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/2306 ,  C12N2501/235 ,  C12N2501/237 ,  C12N2501/26 ,  C12N2501/998 ,  C12N2502/13 ,  C12N2502/99 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2510/00 ,  C12N2510/04 ,  C12N2533/50 ,  C12N2533/90

摘要： This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

摘要翻译： 本公开提供了一种用于培养人多能干细胞的改进系统。 传统上，多能干细胞在一层饲养细胞（如小鼠胚胎成纤维细胞）上培养以防止它们分化。 在这里描述的系统中，饲养细胞的作用由添加到培养环境中的组分代替，其支持快速增殖而不分化。 有效的特征是细胞的合适的支持结构，以及可以在不被另一种细胞类型预处理的情况下可以新鲜添加到培养物中的有效培养基。 在根据本发明的新鲜培养基中培养人胚胎干细胞导致细胞惊人地快速扩张，同时保持分化成代表所有三个胚胎胚层的细胞的能力。 这种新的培养系统允许pPS细胞的大量增殖用于商业生产用于药物筛选和人类治疗的重要产品。

公开(公告)号：US12049618B2

公开(公告)日：2024-07-30

申请号：US16912135

申请日：2020-06-25

申请人： MINERVA BIOTECHNOLOGIES CORPORATION

发明人： Cynthia Bamdad

IPC分类号： C07K16/00 ,  A61K31/7088 ,  C07K16/28 ,  C07K16/30 ,  C12N5/0735 ,  C12N5/074 ,  C12N11/06 ,  C12Q1/6881

CPC分类号： C12N11/06 ,  A61K31/7088 ,  C07K16/2863 ,  C07K16/3092 ,  C12N5/0606 ,  C12N5/0696 ,  C12Q1/6881 ,  C07K2317/35 ,  C07K2317/75 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/727 ,  C12N2533/50 ,  C12Q2600/158 ,  C12Q2600/178

摘要： The present application discloses a method for inducing cells to gain characteristics of naïve stem cell state comprising culturing the cells in the presence of a MUC1* activator.

公开(公告)号：US12037607B2

公开(公告)日：2024-07-16

申请号：US16762447

申请日：2018-11-08

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Raul E. Vizcardo ,  Naritaka Tamaoki ,  Meghan L. Good ,  Nicholas P. Restifo

IPC分类号： C12N5/0789 ,  A61K35/28

CPC分类号： C12N5/0647 ,  A61K35/28 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/135 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/20 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/24 ,  C12N2501/25 ,  C12N2501/415 ,  C12N2502/00 ,  C12N2506/45 ,  C12N2513/00 ,  C12N2531/00

摘要： Disclosed are methods of preparing CD34+CD43+ hematopoietic progenitor cells (HPC) in vitro according to embodiments of the invention. Also disclosed are methods of differentiating CD34+CD43+ hematopoietic progenitor cells to hematopoietic lineage cells according to embodiments of the invention. Also disclosed are methods of treating or preventing a condition in a mammal, e.g., cancer, according to embodiments of the invention.

公开(公告)号：US20190093078A1

公开(公告)日：2019-03-28

申请号：US16120342

申请日：2018-09-03

申请人： Technion Research & Development Foundation Limited

发明人： Michal AMIT ,  Joseph ITSKOVITZ-ELDOR

IPC分类号： C12N5/074

CPC分类号： C12N5/0607 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0606 ,  C12N5/0611 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0696 ,  C12N2500/25 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/10 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2501/998 ,  C12N2533/52

摘要： The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.

公开(公告)号：US20180030415A1

公开(公告)日：2018-02-01

申请号：US15551551

申请日：2016-02-19

申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  UNIVERSITÉ DE NANTES

发明人： Tuan Huy NGUYEN ,  Angélique FOURRIER

IPC分类号： C12N5/071

CPC分类号： C12N5/0672 ,  C12N5/067 ,  C12N2500/98 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/237 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/45 ,  C12N2533/52

摘要： The invention relates to the use of a laminin (LN) as a matrix for hepatic differentiation. The invention also relates to a method for inducing hepatic differentiation comprising the steps of: (i) providing a population of human pluripotent cells, (ii) culturing the population on a support coated with a laminin in a endoderm induction medium to produce a population of human DE cells, (iii) culturing said population of human DE cells on a support coated with a laminin in a hepatic induction medium to produce a population of human hepatoblasts-like cells, and (iv) optionally culturing said population of human hepatoblasts-like cells on a support coated with a laminin in a hepatic maturation medium to produce a population of human hepatocyte-like cells. The invention further relates to a population of human hepatoblasts-like cells or human fetal hepatocyte-like cells obtained by the method of the invention. The invention further relates to a population of human hepatoblasts-like cells expressing HNF4α and expressing substantially AFP for use in a method of treatment of the human body.

公开(公告)号：US09850463B2

公开(公告)日：2017-12-26

申请号：US13756489

申请日：2013-01-31

申请人： The Regents of the University of California ,  University of Southern California

发明人： Sherry Hikita ,  Dennis Clegg ,  Lincoln Johnson ,  Liane Miller ,  Marlene Tsie ,  Chelsea Presbrey ,  Lisa Conti ,  Michelle Maloney ,  David Hinton ,  Qirui Hu

IPC分类号： C12N5/02 ,  C12N5/00 ,  C12N5/10 ,  C12N5/071 ,  C12N15/00 ,  C12N5/079

CPC分类号： C12N5/0621 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2506/02

摘要： The production of high quality retinal pigmented epithelium (RPE) cells is necessary for research and potential therapeutic uses. Especially desirable are methods for the production of RPE cells using xeno-free culture conditions. Disclosed herein are novel methods for the production of RPE cells from pluripotent cells with high yields, including xeno-free production methods. Also provided are methods of efficiently isolating RPE cells from cultures containing heterogeneous cell types, allowing for substantially pure RPE cell cultures to be established. Additionally, novel methods for the cryopreservation of RPE cells are provided.

公开(公告)号：US20170313978A1

公开(公告)日：2017-11-02

申请号：US15517904

申请日：2015-10-07

申请人： Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft ,  University of Bath

发明人： Jichang Wang ,  Zsuzsanna Izsvák ,  Laurence Daniel Hurst

IPC分类号： C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/065 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/2306 ,  C12N2501/2311 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2510/00

摘要： One or more type 7 long terminal repeat (LTR7) nucleic acid sequences of type H human endogenous retroviruses (HERVH) (“LTR7/HERVH nucleic acid sequences”) can be used for identifying primate naïve pluripotent stem cells. LTR7/HERVH-associated transcription can be used as a marker for primate naive pluripotent stem cells. A reporter construct that includes LTR7/HERVH nucleic acid sequences can be used for optimizing culture conditions for naïve primate pluripotent stem cells. A cell growth medium can be used for cultivation of primate naive pluripotent stem cells, which can exhibit elevated levels of LTR7/HERVH-associated transcription in comparison to control cells.

公开(公告)号：US20160319241A1

公开(公告)日：2016-11-03

申请号：US15209776

申请日：2016-07-14

申请人： Technion Research & Development Foundation Limited

发明人： Michal AMIT ,  Joseph ITSKOVITZ-ELDOR

IPC分类号： C12N5/0735 ,  C12N5/071 ,  C12N5/073

CPC分类号： C12N5/0606 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0602 ,  C12N5/0603 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/23 ,  C12N2501/2306 ,  C12N2506/02 ,  C12N2533/52

摘要： Well-defined, xeno-free culture media which comprise a TGF-beta isoform or the chimera formed between IL6 and the soluble IL6 receptor (IL6RIL6), which are capable of maintaining stem cells, and particularly, human embryonic stem cells, in an undifferentiated state are provided. Also provided are cell cultures comprising the culture media and the stem cells and methods of expanding and deriving embryonic stem cells in such well-defined, xeno-free culture media. In addition, the present invention provides methods of differentiating ESCs or EBs formed therefrom for the generation of lineage specific cells.

摘要翻译： 包含TGF-β同种型或在IL6和可溶性IL6受体（IL6RIL6）之间形成的嵌合体）的明确定义的不含异种的培养基，其能够维持干细胞，特别是人胚胎干细胞在未分化 状态。 还提供了包含培养基和干细胞的细胞培养物以及在这种定义良好的不含异种植物的培养基中扩增和衍生胚胎干细胞的方法。 此外，本发明提供了区分由其形成的ESC或用于产生谱系特异性细胞的EBs的方法。

公开(公告)号：US09410121B2

公开(公告)日：2016-08-09

申请号：US14578443

申请日：2014-12-21

申请人： Technion Research & Development Foundation Limited

发明人： Michal Amit ,  Joseph Itskovitz-Eldor

IPC分类号： C12N5/02 ,  C12N5/074 ,  C12N5/0735 ,  C12N5/00 ,  C12N5/0797

CPC分类号： C12N5/0606 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0607 ,  C12N5/0611 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0696 ,  C12N2500/25 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/10 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2501/998 ,  C12N2533/52

摘要： The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.

摘要翻译： 本发明是使用无饲养细胞，不含异种植物的培养系统和干细胞来建立和繁殖人类胚胎干细胞系的方法，所述培养系统和干细胞能够在不含异种的培养物中保持未分化，多能和增殖状态 污染物和饲养细胞。

公开(公告)号：US20160222346A1

公开(公告)日：2016-08-04

申请号：US15050069

申请日：2016-02-22

申请人： ViaCyte, Inc.

发明人： Allan J. Robins ,  Thomas C. Schulz

IPC分类号： C12N5/0735 ,  C12N5/071

CPC分类号： C12N5/0606 ,  C12N5/068 ,  C12N2500/84 ,  C12N2500/98 ,  C12N2501/15 ,  C12N2506/02

摘要： The present invention provides compositions and methods for the culture and maintenance of pluripotent stem cells. More particularly, the present invention provides for compositions and methods for culturing, maintaining, growing and stabilizing primate pluripotent stem cells in a feeder-free defined media further comprising human serum, or a soluble attachment component of the human serum, for promoting cell attachment.

摘要翻译： 本发明提供了用于培养和维持多能干细胞的组合物和方法。 更具体地说，本发明提供了用于培养，保持，生长和稳定灵长类多能干细胞的组合物和方法，所述组合物和方法还包含进料器的限定的培养基，其还包含人血清或人血清的可溶性附着组分，用于促进细胞附着。