公开(公告)号：US20150344876A1

公开(公告)日：2015-12-03

申请号：US14730824

申请日：2015-06-04

Applicant: LIFE TECHNOLOGIES CORPORATION ,  GENEART AG

Inventor： Todd PETERSON ,  Axel Trefzer ,  Thomas Poehmerer

IPC: C12N15/10 ,  B01J19/00

CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B01J2219/00313 ,  B01J2219/00351 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00655 ,  B01J2219/00659 ,  B01J2219/00713 ,  B01J2219/00722 ,  C12N15/10 ,  C12N15/1031 ,  C12N15/1068 ,  C12N15/64 ,  C12N15/66 ,  C12P19/34

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

Abstract translation: 本公开一般涉及用于生产核酸分子的组合物和方法。 在一些方面，本发明允许核酸分子的微量生成，任选地随后将这些核酸分子装配到更大的分子中。 在一些方面，本发明允许有效生产核酸分子（例如，大的核酸分子，例如基因组）。

公开(公告)号：US20150322488A1

公开(公告)日：2015-11-12

申请号：US14730989

申请日：2015-06-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael R. GAMBINI ,  John G. ATWOOD ,  Anthony L. CERRONE ,  Edward J. LAKATOS ,  Eugene F. YOUNG

IPC: C12Q1/68 ,  G01N21/64 ,  B01L7/00

CPC classification number: G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

Abstract translation: 光学仪器监测具有温度循环阻滞的反应装置中DNA的PCR复制，所述反应装置具有小瓶反应成分，包括在双链DNA存在下发荧光的染料。 分束器将激发光束传递到小瓶以使染料发荧光。 来自染料的发射光束通过分束器传递到CCD检测器，处理器从该CCD检测器计算DNA浓度。 具有多个参考发射器的参考条发射不同强度的参考光束，处理器从该参考光束选择用于补偿漂移的最佳发射器。 自动调整曝光时间以保持CCD和处理器的最佳动态范围。 分束器和相关的滤光器的模块与选定的染料相关联，并且可替代不同的染料。

公开(公告)号：US20150278904A1

公开(公告)日：2015-10-01

申请号：US14637587

申请日：2015-03-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John CARRINO ,  Feng Liang ,  Siamak Baharloo ,  Barry Schweitzer

IPC: G06Q30/06

CPC classification number: G06Q30/0627 ,  G06Q30/06 ,  G06Q50/22 ,  Y02A90/22 ,  Y02A90/24

Abstract: Provided herein are collections of matched biological reagents selected from a larger collection of biological reagents, wherein the collection of matched biological reagents relate to a biological element. Also provided are methods for selling an isolated biomolecule or biological research reagent in a collection of matched biological reagents, and methods for selecting an isolated biomolecule or biological research reagent from a collection of biological reagents.

Abstract translation: 本文提供了选自更大量的生物试剂的匹配生物试剂的集合，其中匹配的生物试剂的收集涉及生物学元件。 还提供了在匹配的生物试剂的集合中销售分离的生物分子或生物研究试剂的方法，以及从生物试剂的集合中选择分离的生物分子或生物研究试剂的方法。

公开(公告)号：US20150278437A1

公开(公告)日：2015-10-01

申请号：US14428312

申请日：2013-09-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Nivedita Sumi Majumdar ,  Thomas Wessel ,  David C. Woo ,  Marcin Sikora ,  Gordon A. Janaway ,  Shweta Raizada ,  Joanna Lankester ,  Jeffrey A. Marks ,  Daniel Wessel

IPC: G06F19/20 ,  C12Q1/68

CPC classification number: G16B25/00 ,  C12Q1/686 ,  G16B40/00

Abstract: A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

Abstract translation: 提供了一种用于设计数字PCR（dPCR）实验的计算机实现方法。 该方法包括从用户接收优化类型的选择。 优化类型可以使动态范围最大化，例如最小化包括实验所需的反应位点，确定稀释因子或确定检测下限的底物数量。 该方法还包括从用户接收实验的精确度量，以及用于实验的反应部位中靶的最小浓度。 该方法还包括基于优化类型确定实验的一组dPCR实验设计因子。 然后将该组dPCR实验设计因子显示给用户。

公开(公告)号：US20150275257A1

公开(公告)日：2015-10-01

申请号：US14622653

申请日：2015-02-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark G. Erlander ,  Ranelle C. Salunga ,  Steven T. Kunitake ,  Liang You ,  Theresa B. Taylor

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2525/179 ,  C12Q2525/143 ,  C12Q2521/107

Abstract: The present invention provides improved methods for the amplification of nucleic acid molecules. Methods for amplifying target polynucleotides, including mRNA, using oligonucleotides, DNA and RNA polymerases are provided. The invention further provides compositions and kits for practicing the methods, as well as methods which use the amplification products.

Abstract translation: 本发明提供了用于扩增核酸分子的改进方法。 提供了使用寡核苷酸，DNA和RNA聚合酶扩增靶多核苷酸（包括mRNA）的方法。 本发明还提供了用于实施该方法的组合物和试剂盒，以及使用扩增产物的方法。

公开(公告)号：US20150260680A1

公开(公告)日：2015-09-17

申请号：US14724354

申请日：2015-05-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John DAVIDSON ,  Wolfgang HINZ ,  Jonathan ROTHBERG ,  Richard WHITAKER

IPC: G01N27/414

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  G01N27/4145

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

Abstract translation: 提供了包含修饰的蛋白质，特别是具有改变的缓冲性质的经修饰的核酸结合蛋白质的方法，组合物，系统，装置和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰以获得所需pKa值的修饰蛋白质的方法。 此外，本发明提供了在离子产生反应中使用这些修饰蛋白质的方法，例如基于离子的核酸测序反应。

公开(公告)号：US20150259082A1

公开(公告)日：2015-09-17

申请号：US14728717

申请日：2015-06-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael E. Goodwin ,  Jeremy K. Larsen ,  Patrick L. Draper ,  Brandon M. Knudsen

IPC: B65B1/04 ,  B65B51/22 ,  B65B51/02 ,  B65B3/04

CPC classification number: B65B1/04 ,  A61J1/10 ,  B65B3/003 ,  B65B3/02 ,  B65B3/04 ,  B65B51/02 ,  B65B51/225

Abstract: A fluid manifold system includes a manifold having at least portions of opposing flexible sheets welded together to form a fluid flow path therebetween, a fluid inlet communicating with the fluid flow path. A plurality of receiving containers are in fluid communication with the fluid flow path of the manifold, each receiving container bounding a compartment. The receiving containers can be formed integral with the manifold by welding together a second portion of the opposing flexible sheets or can comprise separate containers that are coupled to the manifold.

Abstract translation: 流体歧管系统包括歧管，歧管具有至少部分相对的柔性片焊接在一起以在其间形成流体流动通道，与流体流动路径连通的流体入口。 多个接收容器与歧管的流体流动路径流体连通，每个接收容器围绕隔室。 接收容器可以通过将相对的柔性片的第二部分焊接在一起而与歧管形成一体，或者可以包括联接到歧管的分开的容器。

公开(公告)号：US09134269B2

公开(公告)日：2015-09-15

申请号：US13794637

申请日：2013-03-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/68 ,  H01L23/00 ,  H01L29/78 ,  H01L21/306

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4148 ,  H01L21/306 ,  H01L24/18 ,  H01L24/20 ,  H01L24/82 ,  H01L27/088 ,  H01L29/78 ,  H01L2224/04105 ,  H01L2224/16 ,  H01L2224/76155 ,  H01L2224/82102 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01011 ,  H01L2924/01012 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01019 ,  H01L2924/0102 ,  H01L2924/01023 ,  H01L2924/01024 ,  H01L2924/01027 ,  H01L2924/01029 ,  H01L2924/0103 ,  H01L2924/01033 ,  H01L2924/01046 ,  H01L2924/01047 ,  H01L2924/01052 ,  H01L2924/01056 ,  H01L2924/01059 ,  H01L2924/01073 ,  H01L2924/01074 ,  H01L2924/01075 ,  H01L2924/01082 ,  H01L2924/1306 ,  H01L2924/13091 ,  H01L2924/14 ,  H01L2924/1433 ,  H01L2924/19041 ,  H01L2924/19042 ,  H01L2924/19043 ,  H01L2924/30105 ,  H01L2924/3011 ,  H01L2924/3025 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101 ,  H01L2924/00

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：USD738243S1

公开(公告)日：2015-09-08

申请号：US29481232

申请日：2014-02-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Saul Selberg ,  Sandro Klein ,  Ronald Parmenter ,  Wesley Smith

公开(公告)号：US09128044B2

公开(公告)日：2015-09-08

申请号：US14198382

申请日：2014-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Keith G. Fife ,  Jordan Owens ,  Shifeng Li ,  James Bustillo

IPC: G01N27/414 ,  B01L3/00

CPC classification number: G01N27/414 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N27/4145 ,  G01N27/4148

Abstract: In one embodiment, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A material defines an opening extending to the upper surface of the floating gate conductor. The material comprises a first dielectric underlying a second dielectric. A conductive element contacts the upper surface of the floating gate conductor and extends a distance along a sidewall of the opening, the distance defined by a thickness of the first dielectric.

Abstract translation: 在一个实施例中，描述了化学传感器。 化学传感器包括具有上表面的浮栅导体的化学敏感场效应晶体管。 材料限定了延伸到浮动栅极导体的上表面的开口。 该材料包括位于第二电介质下面的第一电介质。 导电元件接触浮动栅极导体的上表面并且沿着开口的侧壁延伸一定距离，该距离由第一电介质的厚度限定。