公开(公告)号：US11866771B2

公开(公告)日：2024-01-09

申请号：US17345834

申请日：2021-06-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Benjamin J. Hindson

IPC: C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/686

CPC classification number: C12Q1/6848 ,  C12Q1/686 ,  C12Q1/6816 ,  C12Q1/6846 ,  C12Q1/6846 ,  C12Q1/6846 ,  C12Q2527/125 ,  C12Q2563/159 ,  C12Q1/6848 ,  C12Q1/6846 ,  C12Q2527/125 ,  C12Q2563/159

Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.

公开(公告)号：US11633739B2

公开(公告)日：2023-04-25

申请号：US17137274

申请日：2020-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Donald A. Masquelier ,  Billy W. Colston, Jr. ,  Benjamin J. Hindson

IPC: B01L3/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/10 ,  C12Q1/686 ,  G01N21/64 ,  B29C45/00 ,  G01N21/49 ,  B01F101/23 ,  B29L31/00

Abstract: System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.

公开(公告)号：US20220008914A1

公开(公告)日：2022-01-13

申请号：US17486667

申请日：2021-09-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Donald A. Masquelier ,  Kevin D. Ness ,  Benjamin J. Hindson ,  Anthony J. Makarewicz, JR. ,  Erin R. Chia ,  Billy W. Colston, JR. ,  Serge Saxonov ,  Svilen S. Tzonev ,  Michael Y. Lucero ,  Ryan T. Koehler

IPC: B01L3/00 ,  B01F3/08 ,  B01F13/00 ,  B01F13/10 ,  B01L9/00 ,  C12Q1/686

Abstract: Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.

公开(公告)号：US11130128B2

公开(公告)日：2021-09-28

申请号：US16667811

申请日：2019-10-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Donald A. Masquelier ,  Billy W. Colston, Jr. ,  Benjamin J. Hindson

IPC: B01L3/00 ,  B01F3/08 ,  C12Q1/686 ,  B01F5/00 ,  G01N35/08 ,  B01F5/02 ,  B01F13/00 ,  B01F13/10 ,  B01L9/00

Abstract: Method of detecting a target nucleic acid. In an exemplary method, at least two thermal zones of different temperature may be created using a heating assembly. A first emulsion and a second emulsion may be formed. The first and second emulsions may be thermally cycled by passing them through tubing in a spaced relation to one another, with the tubing being wound around a central axis of the heating assembly and extending through each thermal zone multiple times. Thermally cycling may promote amplification of the target nucleic acid in droplets of each emulsion. Droplets of each emulsion may be passed through a detection channel located downstream of the tubing. Fluorescence may be detected from the droplets being passed through the detection channel.

公开(公告)号：US10677693B2

公开(公告)日：2020-06-09

申请号：US16236116

申请日：2018-12-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Billy W. Colston, Jr. ,  Kevin D. Ness ,  Donald A. Masquelier

IPC: G01N1/30 ,  B01J19/00 ,  C12Q1/6876 ,  G01N21/75 ,  C12Q1/6844 ,  G01N33/50 ,  B01F13/00 ,  B01L3/00 ,  G01N35/08 ,  B01L7/00 ,  G01N1/44 ,  C40B50/08 ,  C40B60/14

Abstract: System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.

公开(公告)号：US10378048B2

公开(公告)日：2019-08-13

申请号：US15452582

申请日：2017-03-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Benjamin J. Hindson

IPC: C12Q1/68 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/686

Abstract: System, including methods, apparatus, compositions, and kits, for making and using a stabilized emulsion. A method of generating a stabilized emulsion is provided. In the method, an aqueous phase may be provided. The aqueous phase may include an effective concentration of one or more skin-forming proteins. An emulsion may be formed. The emulsion may include droplets of a dispersed phase disposed in a continuous phase, with the aqueous phase being the continuous phase or the dispersed phase. The emulsion may be heated to create an interfacial skin between each droplet and the continuous phase, to transform the droplets into capsules.

公开(公告)号：US10279350B2

公开(公告)日：2019-05-07

申请号：US15365894

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Kevin D. Ness ,  Billy W. Colston, Jr. ,  Fred P. Milanovich ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F13/00 ,  B01F15/00 ,  B01L3/02 ,  B01L7/00 ,  B29C45/00 ,  C12Q1/686 ,  G01N21/3563 ,  G01N21/49 ,  G01N21/64 ,  B01F3/08 ,  B29L31/00

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F13/0062 ,  B01F15/00922 ,  B01F2003/0834 ,  B01F2003/0842 ,  B01F2215/0037 ,  B01L3/0241 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0622 ,  B29C45/0053 ,  B29C45/006 ,  B29C2045/0079 ,  B29L2031/752 ,  C12Q1/686 ,  G01N21/3563 ,  G01N21/49 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  Y02A90/26

Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.

公开(公告)号：US10258989B2

公开(公告)日：2019-04-16

申请号：US15365888

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Benjamin J. Hindson ,  Billy W. Colston, Jr. ,  Donald A. Masquelier

IPC: B01L3/00 ,  B01F3/08 ,  B01F13/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  C12Q1/686 ,  G01N21/64 ,  B01F15/00 ,  B29C45/00 ,  G01N21/49 ,  B29L31/00

Abstract: Methods of making a droplet-generating device. In an exemplary method, an upper member is injection molded. The upper member includes a bottom surface and also includes a first microfluidic channel, a second microfluidic channel, and a third microfluidic channel each formed in the bottom surface. The upper member has a plurality of openings each extending completely through the upper member from the bottom surface and creating a side wall region of a sample well, a carrier well, and a droplet well. A cover layer is attached to the bottom surface of the upper member, such that the cover layer seals a bottom side of each microfluidic channel. The microfluidic channels meet one another to create a droplet-generation region. The sample well, the carrier well, and the droplet well are connected to the droplet-generation region via the first, second, and third microfluidic channels, respectively.

公开(公告)号：US20170144116A1

公开(公告)日：2017-05-25

申请号：US15365888

申请日：2016-11-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Benjamin J. Hindson ,  Billy W. Colston, JR. ,  Donald A. Masquelier

IPC: B01F3/08 ,  B01F15/00 ,  B29C45/00 ,  G01N21/49 ,  B01L3/00 ,  B01L7/00 ,  G01N21/64 ,  B01F13/00 ,  C12Q1/68

CPC classification number: B01L3/502784 ,  B01F3/0807 ,  B01F13/0062 ,  B01F15/00922 ,  B01F2003/0834 ,  B01F2003/0842 ,  B01F2215/0037 ,  B01L3/0241 ,  B01L3/502715 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0858 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0622 ,  B29C45/0053 ,  B29C45/006 ,  B29C2045/0079 ,  B29L2031/752 ,  C12Q1/686 ,  G01N21/3563 ,  G01N21/49 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G06F19/00 ,  Y02A90/26

Abstract: Methods of making a droplet-generating device. In an exemplary method, an upper member is injection molded. The upper member includes a bottom surface and also includes a first microfluidic channel, a second microfluidic channel, and a third microfluidic channel each formed in the bottom surface. The upper member has a plurality of openings each extending completely through the upper member from the bottom surface and creating a side wall region of a sample well, a carrier well, and a droplet well. A cover layer is attached to the bottom surface of the upper member, such that the cover layer seals a bottom side of each microfluidic channel. The microfluidic channels meet one another to create a droplet-generation region. The sample well, the carrier well, and the droplet well are connected to the droplet-generation region via the first, second, and third microfluidic channels, respectively.

公开(公告)号：US20140272995A1

公开(公告)日：2014-09-18

申请号：US14216006

申请日：2014-03-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Erin R. Chia ,  Amy L. Hiddessen ,  Benjamin J. Hindson ,  Adam Lowe ,  Chunxiao Han

IPC: C12Q1/68 ,  C07F7/18

CPC classification number: C07F7/1804 ,  C08G77/46 ,  C08G77/50 ,  C08L83/12 ,  C08L83/14 ,  G01N33/5436

Abstract: System, including methods and compositions, for making and using emulsions that include a silicone oil and a silicone surfactant. The emulsions may include aqueous droplets disposed in a continuous phase that includes a silicone oil and a silicone surfactant. The aqueous droplets may contain an analyte, optionally at partial occupancy, and/or a luminescent (e.g., photoluminescent) reporter. An assay of the analyte may be performed with the droplets. In some cases, signals may be detected from the droplets, and a characteristic of the analyte, such as an analyte level or activity, may be determined based on the signals.

Abstract translation: 用于制备和使用包括硅油和硅氧烷表面活性剂的乳液的方法和组合物的系统。 乳液可以包括设置在包括硅油和硅氧烷表面活性剂的连续相中的水性液滴。 水滴可以含有任选地部分占据的分析物和/或发光（例如光致发光）报告物。 分析物的测定可以用液滴进行。 在一些情况下，可以从液滴中检测信号，并且可以基于该信号来确定分析物的特征，例如分析物水平或活性。