公开(公告)号：US09951101B2

公开(公告)日：2018-04-24

申请号：US15033940

申请日：2014-11-06

Applicant: EMD Millipore Corporation

Inventor： Mikhail Kozlov ,  William Cataldo ,  Jeffrey Caron

IPC: C07K1/34 ,  C08F220/56 ,  C08F220/58 ,  B01D15/10 ,  B01D37/00 ,  B01J20/26 ,  B01J20/288 ,  B01J20/289 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/54 ,  C08F259/08 ,  C08J5/22 ,  B01J39/26 ,  B01J20/32

CPC classification number: C07K1/34 ,  B01D15/10 ,  B01D37/00 ,  B01J20/267 ,  B01J20/288 ,  B01J20/289 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/327 ,  B01J20/3276 ,  B01J20/3291 ,  B01J39/26 ,  B01J2220/80 ,  C07K1/18 ,  C07K16/00 ,  C08F220/18 ,  C08F220/54 ,  C08F220/56 ,  C08F220/58 ,  C08F259/08 ,  C08F2220/1825 ,  C08F2220/1858 ,  C08J5/2243 ,  C08J2351/00

Abstract: Novel compositions for removing impurities such as, protein aggregates, from a sample containing a protein of interest, e.g., an antibody. Such compositions can be used prior to the virus filtration step during protein purification, to remove aggregates and protect the virus filter from fouling, therefore improving virus filter capacity. A porous solid support including a co-polymer having at least two monomers, wherein at least one of the monomers comprises acrylamide and at least a second monomer comprises a hydrophobic binding group, where the solid support selectively binds protein aggregates, thereby to separate the monomeric protein of interest from the protein aggregates. The method can be performed under neutral to high pH and high conductivity conditions.

公开(公告)号：US09889214B2

公开(公告)日：2018-02-13

申请号：US15691290

申请日：2017-08-30

Applicant: EMD Millipore Corporation

Inventor： Mikhail Kozlov ,  Wilson Moya ,  Gabriel Tkacik

IPC: B01D39/16 ,  A61L2/00 ,  B01D67/00 ,  B01D69/00 ,  C02F1/44

CPC classification number: A61L2/0017 ,  B01D39/1623 ,  B01D67/0004 ,  B01D67/002 ,  B01D69/00 ,  B01D2239/025 ,  B01D2323/39 ,  C02F1/444 ,  C02F2303/04

Abstract: A method for removing microorganisms from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention. Microorganisms such as bacteria, particularly B. Diminuta, are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having a B. Diminuta LRV greater than about 9, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. Another method for removing microorganisms such as bacteria and Mycloplasma, includes passing the liquid through a porous nanofiber containing filtration medium having a microorganism LRV greater than about 8, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. The filtration medium can be in the form of a fibrous electro spun polymeric nanofiber liquid filtration medium mat.

公开(公告)号：US20170360970A1

公开(公告)日：2017-12-21

申请号：US15691290

申请日：2017-08-30

Applicant: EMD Millipore Corporation

Inventor： Mikhail Kozlov ,  Wilson Moya ,  Gabriel Tkacik

IPC: A61L2/00 ,  B01D39/16 ,  B01D67/00 ,  B01D69/00 ,  C02F1/44

CPC classification number: A61L2/0017 ,  B01D39/1623 ,  B01D67/0004 ,  B01D67/002 ,  B01D69/00 ,  B01D2239/025 ,  B01D2323/39 ,  C02F1/444 ,  C02F2303/04

Abstract: A method for removing microorganisms from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention. Microorganisms such as bacteria, particularly B. Diminuta, are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having a B. Diminuta LRV greater than about 9, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. Another method for removing microorganisms such as bacteria and Mycloplasma, includes passing the liquid through a porous nanofiber containing filtration medium having a microorganism LRV greater than about 8, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. The filtration medium can be in the form of a fibrous electro spun polymeric nanofiber liquid filtration medium mat.

公开(公告)号：US20170360969A1

公开(公告)日：2017-12-21

申请号：US15691286

申请日：2017-08-30

Applicant: EMD Millipore Corporation

Inventor： Mikhail Kozlov ,  Wilson Moya ,  Gabriel Tracik

IPC: A61L2/00 ,  B01D39/16 ,  B01D67/00 ,  B01D69/00 ,  C02F1/44

CPC classification number: A61L2/0017 ,  B01D39/1623 ,  B01D67/0004 ,  B01D67/002 ,  B01D69/00 ,  B01D2239/025 ,  B01D2323/39 ,  C02F1/444 ,  C02F2303/04

Abstract: A method for removing microorganisms from liquid samples and a nanofiber containing liquid filtration medium that simultaneously exhibits high liquid permeability and high microorganism retention. Microorganisms such as bacteria, particularly B. Diminuta, are removed from a liquid by passing the liquid through a porous nanofiber containing filtration medium having a B. Diminuta LRV greater than about 9, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. Another method for removing microorganisms such as bacteria and Mycloplasma, includes passing the liquid through a porous nanofiber containing filtration medium having a microorganism LRV greater than about 8, and the nanofiber(s) has a diameter from about 10 nm to about 1,000 nm. The filtration medium can be in the form of a fibrous electro spun polymeric nanofiber liquid filtration medium mat.

公开(公告)号：US09815050B2

公开(公告)日：2017-11-14

申请号：US14682456

申请日：2015-04-09

Applicant: EMD Millipore Corporation

Inventor： David Yavorsky ,  John Amara ,  Joaquin Umana ,  William Cataldo ,  Mikhail Kozlov ,  Matthew Stone

IPC: B01D15/36 ,  B01J20/28 ,  B01J47/12 ,  B01J39/26 ,  C12N7/02 ,  B01D15/32 ,  B01D15/38 ,  B01J20/285 ,  B01J20/287 ,  B01J20/32 ,  C07K1/18 ,  B01J41/20 ,  C07K16/00 ,  C12N7/00 ,  B01J47/014 ,  B01J47/127

CPC classification number: B01J39/26 ,  B01D15/327 ,  B01D15/361 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  B01J20/28023 ,  B01J20/28059 ,  B01J20/285 ,  B01J20/287 ,  B01J20/3244 ,  B01J41/20 ,  B01J47/014 ,  B01J47/127 ,  C07K1/18 ,  C07K16/00 ,  C12N7/00 ,  C12N7/02 ,  G01N2030/521 ,  Y10S502/52724 ,  Y10T428/2938 ,  Y10T428/2973

Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).

公开(公告)号：US20160051943A1

公开(公告)日：2016-02-25

申请号：US14878030

申请日：2015-10-08

Applicant: EMD Millipore Corporation

Inventor： Mikhail Kozlov ,  Kevin Rautio

IPC: B01D71/56 ,  B01D69/12 ,  C07K1/34 ,  B01D67/00

CPC classification number: B01D71/56 ,  B01D67/0006 ,  B01D67/009 ,  B01D69/125 ,  B01D2323/30 ,  B01D2323/34 ,  B01D2325/14 ,  B01D2325/30 ,  C07K1/24 ,  C07K1/34

Abstract: A negatively charged microporous filtration medium having a high charge density comprising a porous substrate and a polymerized cross-linked polymeric coating located on the inner and outer surfaces of the substrate. The coating may be formed from a reactant solution comprising negatively charged cross-linkable polymerizeable acrylamidoalkyl monomers and acrylamido cross-linking agents which are polymerized in situ on the substrate. The negatively charged microporous filtration medium are suitable for use as prefiltration membranes for selectively removing protein aggregates from a protein solution.