摘要:
The inventive liquid separator of laboratory use can overcome the problems in the conventional separatory funnels used for liquid-liquid phase separation. The inventive apparatus comprises (a) a first bottle-like vessel with a mouth, (b) a second bottle-like vessel with a mouth connected with the first vessel at the mouths with air-tightness but disconnectable therefrom, (c) a porous membrane partitioning the first and the second vessels and made, preferably, of a sintered body of a water-repellent plastic resin such as a fluorocarbon polymer, and (d) an air-passage tubing opening at the ends in the first and the second vessels. When a two-phase mixture of an aqueous liquid and an organic liquid is taken in the first vessel positioned above the second vessel, the organic liquid can pass through the porous membrane to be transferred into the second vessel but the aqueous liquid is retained in the first vessel by virtue of the water-repellency of the fluorocarbon polymer. The air-passage tubing serves as an air escape from the second vessel to the first so as not to disturb the down-flow of the liquid through the porous membrane.
摘要:
Provided is a device for concentrating and separating cells, which has a function for continuously concentrating cells; a function for then continuously arranging the concentrated cells in predetermined regions of a flow path; a function for simultaneously identifying shape and fluorescent emission in one-cell units on the basis of cell concentration and purification images, which serve to continuously separate and purify cells that have different properties in that they are either attracted to or repelled by an induction electrophoresis force of a predetermined frequency; and a function for identifying cells on the basis of this shape and fluorescent emission information and thereby separating and purifying the cells.
摘要:
Provided is a cell concentration and purification device, having: a function of continuously concentrating cells; a function of then subsequently disposing the cells continuously in a specific region of a channel; a function of simultaneously recognizing, based on an image, the shape and fluorescence emission of each single cell; and a function of recognizing the cells and then separating and purifying the same based on the data relating to the shape and fluorescence emission thereof.
摘要:
The present invention provides a liquid reflux reaction control device comprising: a reaction vessel having one or a plurality of wells configured to accommodate a sample; a heat exchange vessel provided in contact with the reaction vessel so as to conduct heat to the reaction vessel, and comprising an inlet and an outlet respectively for introducing and draining a liquid of a predetermined temperature; a plurality of liquid reservoir tanks provided with a temperature-controllable heat source for maintaining liquids of predetermined temperatures; a tubular flow channel that connects the inlet and the outlet of the heat exchange vessel with the liquid reservoir tanks; a pump disposed on the tubular flow channel, and configured to circulate the liquid between the heat exchange vessel and the liquid reservoir tank; and a switching valve disposed on the tubular flow channel, and configured to control the flow of the circulating liquid, which controls the temperature of the reaction vessel to keep a desired temperature by switching the flows of the liquids of the predetermined temperatures from the plurality of liquid reservoir tanks into the heat exchange vessel at a predetermined time interval, wherein the amount of the sample is less than or equal to several μL per well, and the total volume of the circulating liquid is more than or equal to several tens of mL per liquid reservoir tank.
摘要:
In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.
摘要:
A DNA microarray system whereby measurement can be performed at a low running cost, a low price and yet a high accuracy. A nucleic acid probe (3) is immobilized on the surface of a gate insulator of an electric field effect transistor and then hybridized with a target gene on the surface of the gate insulator. A change in the surface electric charge density thus arising is detected by using the electric effect.
摘要:
A convention appliance monitoring apparatus handles only the amount of gas used and security information for the case of a gas cutoff and can not address social needs for a desire to obtain information about influence (e.g., an amount of CO2 emission) of use of the gas combustion appliance on a terrestrial environment.Based on a gas appliance used by a client and a gas flow signal pertaining the gas appliance which are output from an appliance determination unit 2, CO2 emission data on the gas appliance stored in a CO2 emission data storage unit 3, and CO2 emission data which are to be compared with the gas appliance and stored in a comparative CO2 emission data storage unit 4, a CO2 emission calculation unit 5 calculates an amount of CO2 emission, an comparative amount of CO2 emission and a difference between the amount of CO2 emission and the comparative amount of CO2 emission. Accordingly, it is possible to determine the amount of CO2 emission produced by using the gas appliance and the difference between the amount of CO2 emission and the comparative amount of CO2 emission.
摘要:
The present invention provides a method, a chip device, and a system for quantitatively measuring intercellularly and intracellularly-localized mRNA and protein without loss of local space information. In the present invention, cell content is trapped atop a substrate or an electrode in the form of a two-dimensional projection of a cell. Accordingly, electrophoretic force is used, or the cellular moisture content is instantaneously evaporated and immobilized. mRNA immobilized to a two-dimensional surface is identified with a labeled probe which hybridizes to the mRNA.
摘要:
In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.
摘要:
A droplet operation device has a substrate with light transmissibility and having been subjected to a water repellent treatment, a way for supplying droplets onto the substrate, a way for transporting the droplets on the water repellent substrate, and a way for measuring the state of the droplets. The droplet operation device includes the light transmittable substrate, solvent feeding ports for leading reagent or specimen droplets onto the substrate, optical measuring units, and electric field application units. As a result, the inside of the droplets can be observed with a microscope, and results of the reaction of the droplets against other droplets can be measured and classified by stopping and moving the droplets in any direction.