公开(公告)号：US4525276A

公开(公告)日：1985-06-25

申请号：US210545

申请日：1980-11-26

申请人： Shozo Toda ,  Kenji Yasuda ,  Shozo Kokubo

发明人： Shozo Toda ,  Kenji Yasuda ,  Shozo Kokubo

IPC分类号： B01D17/02 ,  B01D31/00

CPC分类号： B01D17/0214

摘要： The inventive liquid separator of laboratory use can overcome the problems in the conventional separatory funnels used for liquid-liquid phase separation. The inventive apparatus comprises (a) a first bottle-like vessel with a mouth, (b) a second bottle-like vessel with a mouth connected with the first vessel at the mouths with air-tightness but disconnectable therefrom, (c) a porous membrane partitioning the first and the second vessels and made, preferably, of a sintered body of a water-repellent plastic resin such as a fluorocarbon polymer, and (d) an air-passage tubing opening at the ends in the first and the second vessels. When a two-phase mixture of an aqueous liquid and an organic liquid is taken in the first vessel positioned above the second vessel, the organic liquid can pass through the porous membrane to be transferred into the second vessel but the aqueous liquid is retained in the first vessel by virtue of the water-repellency of the fluorocarbon polymer. The air-passage tubing serves as an air escape from the second vessel to the first so as not to disturb the down-flow of the liquid through the porous membrane.

摘要翻译： 本发明的实验室用液体分离器可以克服用于液 - 液相分离的常规分离漏斗中的问题。 本发明的装置包括（a）具有口部的第一瓶状容器，（b）第二瓶状容器，其口部具有口气连接的第一容器，气口具有气密性，但可与其断开;（c）多孔 膜分隔第一和第二容器，并且优选地由诸如氟碳聚合物的防水塑料树脂的烧结体制成，以及（d）在第一和第二容器的端部处开口的空气通道管 。 当在位于第二容器上方的第一容器中取出水性液体和有机液体的两相混合物时，有机液体可以通过多孔膜以转移到第二容器中，但水性液体保留在 第一容器借助氟碳聚合物的拒水性。 空气通道管用作从第二容器到第一容器的空气逸出，以便不干扰通过多孔膜的液体的下流。

公开(公告)号：US09447447B2

公开(公告)日：2016-09-20

申请号：US13876992

申请日：2011-09-30

申请人： Kenji Yasuda ,  Tomoyuki Kaneko ,  Fumimasa Nomura

发明人： Kenji Yasuda ,  Tomoyuki Kaneko ,  Fumimasa Nomura

IPC分类号： C12M1/34 ,  C12Q1/02 ,  C12M1/42 ,  C12M1/36 ,  G01N33/50

CPC分类号： C12Q1/025 ,  C12M35/02 ,  C12M41/46 ,  C12M41/48 ,  G01N33/48728 ,  G01N33/5061 ,  G01N33/5088 ,  G01N2800/32

摘要： In the present invention, a cardiomyocyte cluster is disposed on a transparent substrate, and the quality of the cardiomyocytes is evaluated from the response of the cells to a forced pulsation stimulus applied to the cardiomyocytes. The cardiomyocyte cluster is disposed on the transparent substrate, and is exposed to the flow of a liquid containing an agent in a manner so that the agent acts on the cells, which configure a network. The extent of cardiac toxicity resulting from the agent is evaluated from measuring the fluctuations obtained from a comparison of adjacent cardiomyocytes of the network.

摘要翻译： 在本发明中，将心肌细胞簇设置在透明基质上，根据细胞对施加于心肌细胞的强制脉动刺激的反应来评价心肌细胞的质量。 将心肌细胞簇设置在透明基板上，并且以使得试剂作用于构成网络的细胞的方式暴露于含有试剂的液体的流动。 通过测量从网络的相邻心肌细胞的比较获得的波动来评估由该试剂产生的心脏毒性的程度。

公开(公告)号：US08361412B2

公开(公告)日：2013-01-29

申请号：US11317004

申请日：2005-12-27

申请人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

发明人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

IPC分类号： B01L3/00

CPC分类号： G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

摘要： A cell sorting device using an inexpensive chip capable of being exchanged for each sample. The chip includes: a first flow path allowing buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow buffer fluid not containing cells to flow down; a fourth flow path which allows the buffer fluid as a single flow path formed by joining the buffer fluids in the other three flow paths; a cell detecting region for detecting cells flowing with the buffer fluid down the fourth flow path; and a cell sorting region for sorting the cells according to a type of the cells detected. The first to fourth flow paths are cascaded, are supplied with the buffer fluid from reservoirs with the same fluid level, and have substantially the same width or cross-section area.

摘要翻译： 一种使用廉价芯片的细胞分选装置，能够对每个样品进行交换。 芯片包括：允许包含电池的缓冲流体向下流动的第一流动路径; 第二和第三流动路径，其中第一流动路径在其间并且允许不包含电池的缓冲流体向下流动; 第四流路，其允许缓冲流体作为通过连接其它三个流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测沿着第四流动路径流动的缓冲液的细胞; 以及用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一至第四流动路径级联，从具有相同流体水平的储存器提供缓冲流体，并且具有基本上相同的宽度或横截面面积。

公开(公告)号：US08247222B2

公开(公告)日：2012-08-21

申请号：US13036600

申请日：2011-02-28

申请人： Akihiro Hattori ,  Kenji Yasuda

发明人： Akihiro Hattori ,  Kenji Yasuda

IPC分类号： C12M1/00 ,  C12M1/34

CPC分类号： C12M23/16 ,  B01J2219/00317 ,  B01J2219/00441 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00743 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/163

摘要： This invention relates to a method and apparatus for processing cell cultures. The apparatus comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, the absorption layer having absorbency in visible and infrared regions, and the gel-like material being a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and the light source having a monochromatic light in the specific wave length, wherein the light source is disposed such that it irradiates on the absorption layer and/or the gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on the transparent base plate.

摘要翻译： 本发明涉及一种用于处理细胞培养物的方法和装置。 该装置包括一个微室，该微室包括不超过一个吸收层和至少一个凝胶层，依次层叠在透明基板上，在可见光和红外区域中没有明显的吸收性，以及至少一个光源，吸收 在可见光和红外区域具有吸收性的层，凝胶状材料是凝胶溶解温度为100℃以下的物质，加热时的溶胶化物，在室温下处于凝胶状态，具有特定的吸光度 可见光和红外区域的波长，以及具有特定波长的单色光的光源，其中光源被设置成使其照射在吸收层和/或凝胶层上，除了当没有吸收 在透明基板上层叠由凝胶状材料构成的至少两层。

公开(公告)号：US07678777B2

公开(公告)日：2010-03-16

申请号：US12190121

申请日：2008-08-12

申请人： Kenji Yasuda ,  Tomohiko Ogawa ,  Masakatsu Hasegawa

发明人： Kenji Yasuda ,  Tomohiko Ogawa ,  Masakatsu Hasegawa

IPC分类号： A61K31/721 ,  A61K35/74 ,  C08B37/02

CPC分类号： C12N1/38 ,  A23K20/163 ,  A23L29/273 ,  A23L33/135 ,  A23V2002/00 ,  C12N1/20 ,  A23V2200/3204 ,  A23V2250/5042 ,  A23V2200/3202

摘要： A composition for promoting bacterial proliferation and selectively proliferating Lactobacillus casei subsp. casei is disclosed, which includes a dextran. A variety of biological activities originating from L. casei subsp. casei can be sustained in a living body by selectively growing-proliferating and colonizing L. casei subsp. casei in the intestine of a human being, animal, or the like or by selectively growing-proliferating L. casei subsp. casei in the intestine, without supplying L. casei subsp. casei at all times.

摘要翻译： 用于促进细菌增殖和选择性增殖的干酪乳杆菌亚种的组合物。 公开了干酪素，其包括葡聚糖。 来自干酪乳杆菌亚种的各种生物活性 干酪素可以通过选择性生长增殖和殖民化的干酪乳杆菌亚种，维持在活体内。 在人，动物等的肠中或通过选择性增殖增殖的干酪乳杆菌 肠道中的酪蛋白，不提供干酪乳杆菌。 干酪素在任何时候。

公开(公告)号：US20080003678A1

公开(公告)日：2008-01-03

申请号：US11819681

申请日：2007-06-28

申请人： Akihiro Hattori ,  Hideyuki Terazono ,  Kenji Yasuda

发明人： Akihiro Hattori ,  Hideyuki Terazono ,  Kenji Yasuda

IPC分类号： C12N5/06 ,  C12M1/00

CPC分类号： B01L3/502761 ,  B01L3/502753 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  C12M45/00 ,  C12M47/04 ,  G01N15/1031 ,  G01N15/12 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1484 ,  G01N2015/1081 ,  G01N2015/149

摘要： An inexpensive cell analysis and separation apparatus using a flow channel formed on one surface of a substrate and a chip replaceable for each sample, and a method for culturing the separated cells without contamination, are provided. A flow channel for allowing a cell-containing buffer solution to flow is provided. Cells are detected in the middle of the flow channel, and separated to a plurality of downstream flow channels based on whether each cell fulfills a predetermined condition. A culturing tank for collecting the condition-fulfilling cells is covered with a semipermeable membrane at a top surface so as to prevent contamination during cell separation. When the cell separation is finished, the flow channel communicated with the culturing tank accommodating the condition-fulfilling cells is closed, and the culturing tank is cut off from the apparatus and put into a culturing device containing a predetermined medium to culture the cells.

摘要翻译： 提供了使用形成在基板的一个表面上的流动通道和可替代每个样品的芯片的便宜的电池分析和分离装置，以及用于培养分离的电池而不被污染的方法。 提供了允许含细胞的缓冲溶液流动的流动通道。 基于每个小区是否满足预定条件，在流信道的中间检测小区，并将其分离成多个下游流信道。 用于收集状态满足细胞的培养槽在顶表面被半透膜覆盖，以防止细胞分离过程中的污染。 当细胞分离完成时，与容纳条件满足细胞的培养箱连通的流动通道关闭，并将培养箱从装置中切下并放入含有预定培养基的培养装置中以培养细胞。

公开(公告)号：US07217573B1

公开(公告)日：2007-05-15

申请号：US09678652

申请日：2000-10-04

申请人： Yoshitada Oshida ,  Toshihiko Nakata ,  Tomoaki Sakata ,  Kenji Yasuda ,  Satoshi Takahashi

发明人： Yoshitada Oshida ,  Toshihiko Nakata ,  Tomoaki Sakata ,  Kenji Yasuda ,  Satoshi Takahashi

IPC分类号： G01N33/00 ,  G01N33/53 ,  C12Q1/68 ,  C12M3/00 ,  C07H21/04 ,  H01J3/14

CPC分类号： G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  Y10S436/80 ,  Y10T436/143333

摘要： A method of inspecting a DNA chip and an apparatus therefor that allow a picture to be reconstructed in the following steps: A plurality of irradiation spots are formed on a DNA probe array mounted on a stage. Then, the stage is displaced in X, Y directions so as to execute a scanning, thereby irradiating substantially all the entire surface of the DNA probe array. Next, a plurality of emitted fluorescent lights, which are generated from the plurality of irradiation spot portions on the DNA probe array, are converged and are then detected simultaneously by multi detectors. Finally, a data processing apparatus processes the detected signals, thereby reconstructing the picture.

摘要翻译： 一种用于检查DNA芯片的方法及其装置，其允许以下列步骤重建图像：在安装在平台上的DNA探针阵列上形成多个照射点。 然后，在X，Y方向上移位台，以进行扫描，从而基本上照射DNA探针阵列的整个表面。 接下来，从DNA探针阵列上的多个照射点部分产生的多个发射荧光灯被会聚，然后由多个检测器同时检测。 最后，数据处理装置处理检测到的信号，从而重建图像。

公开(公告)号：US20060194307A1

公开(公告)日：2006-08-31

申请号：US11317004

申请日：2005-12-27

申请人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

发明人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

IPC分类号： C12M1/34 ,  B07C5/00

CPC分类号： G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

摘要： The present invention provides a cell sorting device using an inexpensive chip capable of being exchanged for each sample for cell sorting. The cell sorting device uses flow paths formed on the substrate and has the following structure. The chip includes: a first flow path allowing a buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow a buffer fluid not containing cells to flow down from both sides of the first flow path; a fourth flow path which allows the buffer fluid as a single flow path formed by the join of the buffer fluid in the first flow path and the buffer fluid in the second and third flow paths; a cell detecting region for detecting cells flowing with a buffer fluid down the fourth flow path; a cell sorting region for sorting the cells according to a type of the cells detected. The first flow path, the second and the third flow paths, the fourth flow path, the cell detecting region and the cell sorting region provided in a cascade. Two flow paths are provided to allow the cells sorted to flow downstream of the cell sorting region. The buffer fluid flowing in the first to the fourth flow paths is supplied from reservoirs having the same fluid level. The first flow path and the fourth flow path are structured to have substantially the same width or cross-section area.

摘要翻译： 本发明提供一种使用廉价的芯片的细胞分选装置，其能够用于每个样品进行细胞分选。 细胞分选装置使用形成在基板上的流路，具有以下结构。 芯片包括：允许包含电池的缓冲流体向下流动的第一流路; 第二和第三流动路径，其将第一流动路径放置在其间并允许不含有电池的缓冲流体从第一流动路径的两侧向下流动; 第四流动路径，其允许缓冲流体作为由第一流动路径中的缓冲流体的连接和第二和第三流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测在第四流路下方以缓冲液流动的细胞; 用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一流路，第二流路，第三流路，第四流路，单元检测区域和电池排列区域。 提供了两个流动路径以允许排序的细胞流动到细胞分选区域的下游。 在第一至第四流动路径中流动的缓冲流体从具有相同流体液位的储存器供应。 第一流路和第四流路被构造成具有基本相同的宽度或横截面面积。

公开(公告)号：US20060141619A1

公开(公告)日：2006-06-29

申请号：US10544799

申请日：2004-02-19

申请人： Akihiro Hattori ,  Kenji Yasuda

发明人： Akihiro Hattori ,  Kenji Yasuda

IPC分类号： C12N5/06 ,  C12M1/00

CPC分类号： C12M23/16 ,  B01J2219/00317 ,  B01J2219/00441 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00743 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/163

摘要： There is provided a processing apparatus and a processing method for micro-chamber for cell culture having a desired culturing space. The micro-chamber processing apparatus for cell culture of the present invention comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, said absorption layer having absorbency in visible and infrared regions, and said gel-like material being a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and said light source having a monochromatic light in said specific wave length, wherein said light source is disposed such that it irradiates on said absorption layer and/or said gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on said transparent base plate.

摘要翻译： 提供了具有期望的培养空间的用于细胞培养的微室的处理装置和处理方法。 本发明的细胞培养用微细胞处理装置，具有不超过一个吸收层和至少一层凝胶层的微室，依次层叠在透明基板上，在可见光和红外线中不具有明显的吸收性 区域和至少一个光源，所述吸收层在可见光和红外区域具有吸收性，所述凝胶状材料是凝胶溶解温度为100℃以下的物质，加热时的溶胶化物 在具有特定波长的可见光和红外区域具有吸收能力，并且所述光源具有所述特定波长的单色光，其中所述光源被设置成使得其照射在所述吸收层和/或 所述凝胶层除了没有设置吸收层之外，将由凝胶状材料构成的至少两层层合在所述透明体b上 灰板

公开(公告)号：US20050170347A1

公开(公告)日：2005-08-04

申请号：US10499005

申请日：2001-12-19

申请人： Yuji Miyahara ,  Kenji Yasuda ,  Kumiko Hattori

发明人： Yuji Miyahara ,  Kenji Yasuda ,  Kumiko Hattori

IPC分类号： B01J19/00 ,  B01L3/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  C40B40/06 ,  G01N27/414 ,  H01L29/06 ,  H01L29/66 ,  C12M1/34

CPC分类号： B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01L3/5085 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/06 ,  G01N27/4145 ,  C12Q2565/501 ,  C12Q2565/607

摘要： A DNA microarray system whereby measurement can be performed at a low running cost, a low price and yet a high accuracy. A nucleic acid probe (3) is immobilized on the surface of a gate insulator of an electric field effect transistor and then hybridized with a target gene on the surface of the gate insulator. A change in the surface electric charge density thus arising is detected by using the electric effect.

摘要翻译： 一种DNA微阵列系统，其可以以低运行成本，低价格和高精度进行测量。 将核酸探针（3）固定在电场效应晶体管的栅极绝缘体的表面上，然后与栅极绝缘体的表面上的目标基因杂交。 通过使用电效应来检测由此产生的表面电荷密度的变化。