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    Method for nucleic acids isolation
    41.
    发明授权
    Method for nucleic acids isolation 有权
    核酸分离方法

    公开(公告)号：US08809519B2

    公开(公告)日：2014-08-19

    申请号：US12747802

    申请日：2008-12-12

    申请人： Jesus Ching

    发明人： Jesus Ching

    IPC分类号： C07H21/00 ,  C07C315/00 ,  C07C317/00

    CPC分类号： B01J20/3251 ,  B01D15/424 ,  B01J20/286 ,  C12N15/1006

    摘要： Methods for isolating nucleic acids using a solid phase having alkylene sulfonyl-containing compounds on its surface are provided. In one embodiment, the method comprises: contacting a sample containing nucleic acids with a solid phase having alkylene sulfonyl-containing compounds on its surface in a first aqueous solution to provide a loaded solid phase; washing the loaded solid phase with a second aqueous solution to provide a washed solid phase; and eluting the washed solid phase with a low ionic strength liquid to obtain the isolated nucleic acids. Kits containing a solid phase having alkylene sulfonyl-containing compounds also are provided.

    摘要翻译： 提供了使用其表面上含亚烷基磺酰基化合物的固相分离核酸的方法。 在一个实施方案中，所述方法包括：使含有核酸的样品与其表面上含有亚烷基磺酰基的化合物在第一水溶液中接触以提供负载的固相; 用第二水溶液洗涤负载的固相以提供洗涤的固相; 并用低离子强度液体洗脱洗涤的固相以获得分离的核酸。 也提供了含有含亚烷基磺酰基化合物的固相的试剂盒。

    METHOD FOR SEPARATING AN ANALYTE FROM A SAMPLE
    46.
    发明申请
    METHOD FOR SEPARATING AN ANALYTE FROM A SAMPLE 有权
    从样品中分离分析物的方法

    公开(公告)号：US20100068706A1

    公开(公告)日：2010-03-18

    申请号：US12370527

    申请日：2009-02-12

    申请人： Farzad Pourahmadi ,  William A. McMillan ,  Jesus Ching ,  Ronald Chang ,  Lee A. Christel ,  Gregory T.A. Kovacs ,  M. Allen Northrup ,  Kurt E. Petersen

    发明人： Farzad Pourahmadi ,  William A. McMillan ,  Jesus Ching ,  Ronald Chang ,  Lee A. Christel ,  Gregory T.A. Kovacs ,  M. Allen Northrup ,  Kurt E. Petersen

    IPC分类号： C07H1/06 ,  C12Q1/68

    CPC分类号： C12N15/1017 ,  B01D15/00 ,  B01L7/52 ,  B01L2400/0622 ,  B01L2400/0644 ,  C12M47/06 ,  G01N1/34 ,  Y10T436/255

    摘要： An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.

    摘要翻译： 将样品通过将样品引入具有样品端口和从样品端口延伸的第一流路的盒中而将分析物与流体样品分离。 第一流路包括提取室，其包含用于从样品捕获分析物的固体支持物。 所述筒具有用于从所述提取室洗脱所捕获的分析物的第二流动路径，所述第二流在通过所述提取室之后从所述第一流动路径发散。 样品被迫流过提取室并进入废物室，从而当样品流过提取室时用固体支持物捕获分析物。 然后通过迫使洗脱液体流过提取室并沿着第二流动路径，将捕获的分析物从萃取室洗脱。