Hardening method for gelatin
    42.
    发明授权
    Hardening method for gelatin 失效
    明胶硬化方法

    公开(公告)号:US4673632A

    公开(公告)日:1987-06-16

    申请号:US726441

    申请日:1985-04-23

    摘要: A hardening method for gelatin which comprises using as a hardening agent a compound represented by formula (I) ##STR1## wherein R.sup.1, R.sup.2, R.sup.3, and R.sup.4 each represents a substituted or unsubstituted alkyl group, a substituted or unsubstituted alkenyl group, a substituted or unsubstituted aralkyl group, or a substituted or unsubstituted aryl group; or two of said R.sup.1, R.sup.2, R.sup.3, and R.sup.4 are combined with each other to form a ring (1); or 3 or more of said R.sup.1, R.sup.2, R.sup.3 and R.sup.4 are combined with each other to form a condensed ring (2); X represents a group capable of being released when the compound shown by formula (I) reacts with a nucleophilic reagent; Y.sup..crclbar. represents an anion; or Y.sup..crclbar. is combined with one of X, R.sup.1, R.sup.2, R.sup.3, and R.sup.4, ring (1), or ring (2) to form an anionic portion of an intramolecular salt.

    摘要翻译: 一种用于明胶的硬化方法,其包括使用由式(I)表示的化合物作为硬化剂,其中R1,R2,R3和R4各自表示取代或未取代的烷基,取代或未取代的烯基 取代或未取代的芳烷基或取代或未取代的芳基; 或所述R1,R2,R3和R4中的两个相互结合形成环(1); 或所述R1,R2,R3和R4中的3个或更多个彼此组合以形成稠环(2); 当式(I)所示的化合物与亲核试剂反应时,X表示能够释放的基团; Y( - )表示阴离子; 或Y( - )与X,R 1,R 2,R 3和R 4,环(1)或环(2)中的一个结合形成分子内盐的阴离子部分。

    B CELL-DERIVED IPS CELLS AND APPLICATION THEREOF
    47.
    发明申请
    B CELL-DERIVED IPS CELLS AND APPLICATION THEREOF 审中-公开
    B细胞衍生的IPS细胞及其应用

    公开(公告)号:US20110231944A1

    公开(公告)日:2011-09-22

    申请号:US13062439

    申请日:2009-09-04

    摘要: Provided are a B cell-derived iPS cell generated using a convenient technique, a technology for providing a human antibody at low cost using the iPS cell, an immunologically humanized mouse prepared using cells differentiated from the iPS cell, and the like. Also provided are a cloned cell obtained by contacting a B cell with nuclear reprogramming factors excluding C/EBPα and Pax5 expression inhibiting substances, particularly nucleic acids that encode Oct3/4, Sox2, Klf4 and c-Myc, wherein the cloned cell has an immunoglobulin gene rearranged therein and possesses pluripotency and replication competence (B-iPS cell). Still also provided are a method of producing a monoclonal antibody against a specified antigen, comprising recovering an antibody from a culture of B cells obtained by differentiating a B-iPS cell derived from a B cell immunized with the specified antigen, and a method of generating an immunologically humanized mouse, comprising transplanting to an immunodeficient mouse a human immunohematological system cell obtained by differentiating a B-iPS cell.

    摘要翻译: 提供了使用方便的技术产生的B细胞衍生的iPS细胞,使用iPS细胞以低成本提供人抗体的技术,使用从iPS细胞分化的细胞制备的免疫人源化小鼠等的技术。 还提供了通过使B细胞与不包括C /EBPα和Pax5表达抑制物质,特别是编码Oct3 / 4,Sox2,Klf4和c-Myc的核酸的核重编程因子接触获得的克隆细胞,其中克隆的细胞具有免疫球蛋白 基因重排并具有多能性和复制能力(B-iPS细胞)。 还提供了针对特定抗原产生单克隆抗体的方法,包括从通过分化来源于用特定抗原免疫的B细胞的B-iPS细胞获得的B细胞的培养物中回收抗体,以及产生 一种免疫人源化的小鼠,包括将免疫缺陷小鼠移植到通过分化B-iPS细胞获得的人免疫血液学系统细胞中。

    APPARATUS AND METHOD FOR CLEANING SEMICONDUCTOR SUBSTRATE
    49.
    发明申请
    APPARATUS AND METHOD FOR CLEANING SEMICONDUCTOR SUBSTRATE 有权
    用于清洁半导体基板的装置和方法

    公开(公告)号:US20110067734A1

    公开(公告)日:2011-03-24

    申请号:US12845371

    申请日:2010-07-28

    IPC分类号: B08B3/00

    摘要: A method of cleaning a semiconductor substrate includes cleaning a semiconductor substrate with a cleaning liquid, rinsing the semiconductor substrate with rinse water after the semiconductor substrate has been cleaned, and drying the semiconductor substrate after the semiconductor substrate has been rinsed. A temperature of the rinse water is set to 70° C. or above in the rinsing, and the rinse water is set so as to be acidic.

    摘要翻译: 清洗半导体衬底的方法包括用清洗液清洗半导体衬底,在半导体衬底被清洁之后用冲洗水冲洗半导体衬底,以及在半导体衬底被冲洗之后干燥半导体衬底。 冲洗水的温度在漂洗中设定为70℃以上,冲洗水设定为酸性。