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公开(公告)号：US20140234905A1
公开(公告)日：2014-08-21
申请号：US14226579
申请日：2014-03-26
申请人： AbbVie Inc.
发明人： Itzcoatl A. Pla , Joseph G. Matuck , John C. Fann , Christof Schulz , Nichole A. Roy , David F. Bruton , James McIntire , Yu-Hsiang David Chang , Thomas Seewoester
CPC分类号： C12N5/0037 , C07K16/241 , C07K16/244 , C07K16/2863 , C07K2317/14 , C07K2317/21 , C12N5/0043 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/38 , C12N2500/60 , C12N2500/74 , C12N2500/76 , C12N2501/105 , C12N2501/33
摘要： The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.
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公开(公告)号：US08741635B2
公开(公告)日：2014-06-03
申请号：US11596397
申请日：2005-05-12
IPC分类号： C12N5/00
CPC分类号： G01N33/5073 , C12N5/0631 , C12N2501/01 , C12N2501/11 , C12N2501/33 , C12N2501/39
摘要： The present invention relates generally to a method for the generation of a substantially homogenous population of undifferentiated cells. More particularly, the present invention relates to a method for isolating a substantially homogenous population of stem cells, and in particular, mammary stem cells (MaSCs). The MaSCs of the present invention are isolated on the basis of differential levels of proteins present on their cell surface. The MaSCs of the present invention are particularly useful as targets for identifying agents which modulate MaSC survival, self-renewal, proliferation and/or differentiation in both normal and diseased tissue such as, but not limited to, tumor tissue, and, also as source of tissue for the regeneration, replacement and/or augmentation of tissue damaged and/or lost after disease or injury.
摘要翻译： 本发明一般涉及产生基本均匀的未分化细胞群的方法。 更具体地说，本发明涉及用于分离基本均匀的干细胞群，特别是乳腺干细胞（MaSCs）的方法。 基于存在于其细胞表面上的蛋白质的差异水平分离本发明的MaSC。 本发明的MaSCs特别可用作鉴定调节正常组织和患病组织中MaSC存活，自我更新，增殖和/或分化的药剂的靶标，所述组织例如但不限于肿瘤组织，以及作为来源 的组织用于在疾病或损伤后损伤和/或丢失的组织的再生，置换和/或增加。
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公开(公告)号：US20140134724A1
公开(公告)日：2014-05-15
申请号：US13833545
申请日：2013-03-15
申请人： GRIFOLS, S. A.
IPC分类号： C12N5/0775
CPC分类号： C12N5/0668 , C12N5/0663 , C12N2500/05 , C12N2501/115 , C12N2501/33 , C12N2502/115
摘要： A culture medium for human mesenchymal stem cells (hMSC) includes a mesenchymal stem cell basal medium; human leucocyte/platelet coat lysate; insulin; sodium selenite; ethanolamine; and basic fibroblast growth factor. This culture medium is effective for growing hMSC lines, including those which do not grow in culture medium normally used for this type of cell.
摘要翻译： 人类间充质干细胞（hMSC）的培养基包括间充质干细胞基础培养基; 人白细胞/血小板包衣裂解物; 胰岛素; 亚硒酸钠 乙醇胺; 和碱性成纤维细胞生长因子。 这种培养基对生长hMSC系有效，包括那些在这种细胞通常使用的培养基中不生长的细胞系。
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44.发明授权Methods of producing anti-TNF-alpha antibodies in mammalian cell culture 有权在哺乳动物细胞培养物中产生抗TNF-α抗体的方法公开(公告)号：US08663945B2
公开(公告)日：2014-03-04
申请号：US13308075
申请日：2011-11-30
申请人： Itzcoatl A. Pla , Joseph C. Matuck , John C. Fann , Christof Schulz , Nicole A. Roy , David F. Bruton , James McIntire , David Chang Yu-Hsiang , Thomas Seewoester
发明人： Itzcoatl A. Pla , Joseph C. Matuck , John C. Fann , Christof Schulz , Nicole A. Roy , David F. Bruton , James McIntire , David Chang Yu-Hsiang , Thomas Seewoester
CPC分类号： C12N5/0037 , C07K16/00 , C07K16/241 , C07K16/244 , C07K16/2869 , C07K2317/14 , C07K2317/21 , C12N5/0031 , C12N5/0603 , C12N2500/24 , C12N2500/25 , C12N2500/30 , C12N2500/32 , C12N2500/34 , C12N2500/38 , C12N2500/50 , C12N2500/60 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2501/10 , C12N2501/33 , C12N2510/02 , C12N2511/00
摘要： The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.
摘要翻译： 本发明描述了用于在哺乳动物细胞培养物中产生重组蛋白例如抗体的改进的方法和组合物。 此外，本发明提供改进的细胞培养基，包括改良的生产培养基，饲料溶液和组合饲料，其可用于改善哺乳动物细胞培养物中的蛋白质生产力。
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45.发明申请METHODS OF CULTURING CELLS IN A MEDIUM COMPRISING TRANSFORMING GROWTH FACTOR BETA 1 AND BASIC FIBROBLAST GROWTH FACTOR 有权培养细胞培养细胞的方法包括转化生长因子BETA 1和基本成纤维细胞生长因子公开(公告)号：US20140045266A1
公开(公告)日：2014-02-13
申请号：US14058347
申请日：2013-10-21
IPC分类号： C12N5/074
CPC分类号： C12N5/0606 , C12N5/0031 , C12N5/0037 , C12N5/0043 , C12N5/0056 , C12N5/0607 , C12N5/0611 , C12N5/0623 , C12N5/0647 , C12N5/0696 , C12N2500/25 , C12N2500/84 , C12N2500/90 , C12N2500/98 , C12N2501/10 , C12N2501/115 , C12N2501/119 , C12N2501/125 , C12N2501/15 , C12N2501/155 , C12N2501/235 , C12N2501/33 , C12N2501/998 , C12N2533/52
摘要： The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
摘要翻译： 本发明是使用无饲养细胞，不含异种植物的培养系统和干细胞来建立和繁殖人类胚胎干细胞系的方法，所述培养系统和干细胞能够在不含异种的培养物中保持未分化，多能和增殖状态 污染物和饲养细胞。
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公开(公告)号：US20140017789A1
公开(公告)日：2014-01-16
申请号：US13932562
申请日：2013-07-01
IPC分类号： C12N5/077
CPC分类号： C12N5/0667 , A61K35/28 , A61K35/35 , C12N5/0653 , C12N2500/84 , C12N2500/98 , C12N2501/01 , C12N2501/02 , C12N2501/33 , C12N2501/385 , C12N2501/39 , C12N2501/395 , C12N2506/1384 , G01N33/502 , G01N33/5044 , Y02A50/395
摘要： Methods of developing and using cell lines, such as stem cell lines, for therapeutic or cosmetic use. In one embodiment the cell lines are used to treat a wide range of degenerative and metabolic disorders including, but not limited to, obesity, diabetes, hypertension, and cardiac deficiency. Also described are methods of using such cell lines to screen for compounds that play a role in regulating a variety of processes.
摘要翻译： 开发和使用细胞系，如干细胞系，用于治疗或化妆品使用的方法。 在一个实施方案中，细胞系用于治疗广泛范围的退行性和代谢性疾病，包括但不限于肥胖症，糖尿病，高血压和心脏不足。 还描述了使用这种细胞系筛选在调节各种过程中发挥作用的化合物的方法。
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公开(公告)号：US20130316452A1
公开(公告)日：2013-11-28
申请号：US13858534
申请日：2013-04-08
发明人： Irina V. KLIMANSKAYA , Robert LANZA
IPC分类号： C12N5/079
CPC分类号： C12N5/0621 , A61K35/12 , A61K35/30 , A61K35/36 , A61K35/44 , A61K35/545 , C12N5/062 , C12N2500/32 , C12N2500/44 , C12N2501/01 , C12N2501/115 , C12N2501/15 , C12N2501/155 , C12N2501/235 , C12N2501/33 , C12N2501/60 , C12N2506/02
摘要： This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (RPE) cells and other retinal progenitor cells.
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公开(公告)号：US20130302288A1
公开(公告)日：2013-11-14
申请号：US13865751
申请日：2013-04-18
发明人： Irina V. KLIMANSKAYA , Robert LANZA
IPC分类号： A61K35/30
CPC分类号： C12N5/0621 , A61K35/12 , A61K35/30 , A61K35/36 , A61K35/44 , A61K35/545 , C12N5/062 , C12N2500/32 , C12N2500/44 , C12N2501/01 , C12N2501/115 , C12N2501/15 , C12N2501/155 , C12N2501/235 , C12N2501/33 , C12N2501/60 , C12N2506/02
摘要： This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (RPE) cells and other retinal progenitor cells.
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公开(公告)号：US08580248B2
公开(公告)日：2013-11-12
申请号：US10599518
申请日：2005-03-30
申请人： Robert Bartlett Elliott , Olga Garkavenko , Alfred Vasconcellos , Dwaine Emerich , Chris Thanos
发明人： Robert Bartlett Elliott , Olga Garkavenko , Alfred Vasconcellos , Dwaine Emerich , Chris Thanos
CPC分类号： C12N5/067 , A61K2035/126 , C12N2501/33 , C12N2501/335 , C12N2501/39 , C12N2502/1323 , C12N2533/74
摘要： The invention relates to an improved method of culturing hepatocyte cells and non-hepatocyte cells that are capable of secreting liver secretory factors and their use in implantable compositions for treating liver diseases and disorders in patients in need thereof.
摘要翻译： 本发明涉及一种能够分泌肝脏分泌因子的肝细胞和非肝细胞培养物的改进方法及其在可植入组合物中用于治疗有需要的患者的肝病和疾病的用途。
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50.发明授权Methods of culturing cells in a medium comprising transforming growth factor beta 1 and basic fibroblast growth factor 有权在包含转化生长因子β1和碱性成纤维细胞生长因子的培养基中培养细胞的方法公开(公告)号：US08563311B2
公开(公告)日：2013-10-22
申请号：US13466161
申请日：2012-05-08
IPC分类号： C12N15/00
CPC分类号： C12N5/0606 , C12N5/0031 , C12N5/0037 , C12N5/0043 , C12N5/0056 , C12N5/0607 , C12N5/0611 , C12N5/0623 , C12N5/0647 , C12N5/0696 , C12N2500/25 , C12N2500/84 , C12N2500/90 , C12N2500/98 , C12N2501/10 , C12N2501/115 , C12N2501/119 , C12N2501/125 , C12N2501/15 , C12N2501/155 , C12N2501/235 , C12N2501/33 , C12N2501/998 , C12N2533/52
摘要： The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
摘要翻译： 本发明是使用无饲养细胞，不含异种植物的培养系统和干细胞来建立和繁殖人类胚胎干细胞系的方法，所述培养系统和干细胞能够在不含异种的培养物中保持未分化，多能和增殖状态 污染物和饲养细胞。
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