公开(公告)号：US20040195099A1

公开(公告)日：2004-10-07

申请号：US10407560

申请日：2003-04-04

Inventor： Stephen C. Jacobson ,  J. Michael Ramsey

IPC: G01N027/453 ,  B01D061/42

CPC classification number: G01N27/44743 ,  B01D57/02 ,  B01D61/18 ,  G01N30/6095 ,  G01N2030/0035

Abstract: Disclosed are microfluidic devices and methods for performing analyses which, in a preferred embodiment, involve sample filtration, solid phase extraction for enrichment of target analytes and separation of target analytes via open channel electrochromatography. Small sample volumes of mixed analytes are resolvable in less than one minute using the disclosed devices and methods.

Abstract translation: 公开了用于进行分析的微流体装置和方法，其在优选实施方案中涉及样品过滤，用于浓缩目标分析物的固相萃取和通过开放通道电色谱法分离目标分析物。 使用所公开的装置和方法，少于1分钟的混合分析物的小样本体积是可分辨的。

公开(公告)号：US20040136875A1

公开(公告)日：2004-07-15

申请号：US10656535

申请日：2003-09-05

Inventor： Michael Seul ,  Chiu Wo Chau

IPC: B01L003/00

CPC classification number: H05H3/04 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00572 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00713 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0652 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0454 ,  B01L2400/086 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/00 ,  C40B60/10 ,  G01N15/0266 ,  G01N30/0005 ,  G01N33/54313 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2030/0035 ,  G02B3/0012 ,  G02B3/0056 ,  Y10T436/25125 ,  C12Q2565/501 ,  C12Q2563/155 ,  C12Q2565/607

Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components. The invention is also for a system and method for programmable illumination pattern generation, including a novel method and apparatus to generate patterns of illumination and project them onto planar surfaces or onto planar interfaces such as the interface formed by an electrolyte-insulator-semiconductor (EIS), e.g., as described herein. This enables the creation of patterns or sequences of patterns using graphical design or drawing software on a personal computer and the projection of said patterns, or sequences of patterns (nulltime-varying patternsnull), onto the interface using a liquid crystal display (LCD) panel and an optical design which images the LCD panel onto the surface of interest. The use of the LCD technology provides flexibility and control over spatial layout, temporal sequences and intensities (nullgray scalesnull) of illumination patterns. The latter capability permits the creation of patterns with abruptly changing light intensities or patterns with gradually changing intensity profiles.

公开(公告)号：US06749733B1

公开(公告)日：2004-06-15

申请号：US09547049

申请日：2000-04-10

Applicant: Scott S. Sibbett

Inventor： Scott S. Sibbett

IPC: G01N27447

CPC classification number: G01N30/0005 ,  G01N27/44773 ,  G01N30/02 ,  G01N2030/0035 ,  G01N2030/528 ,  B01D15/34

Abstract: The present invention relates to a method of classifying charged molecules such as proteins for quantitative analysis. An analyte solution of the molecules is subjected to separational forces may be fluid drag and electrophoretic force in opposition. The analyte solution may be subjected to a two-phase process. The two-phase process may add both electrophoretic force based upon molecule charge, and differential mobility resistance based upon molecule mass and/or size.

Abstract translation: 本发明涉及对带电分子如蛋白质进行分类的定量分析方法。 分子的分析物溶液经受分离力可能是相反的流体阻力和电泳力。 分析物溶液可以进行两相处理。 两相方法可以基于分子电荷增加电泳力，并且基于分子质量和/或尺寸增加差动迁移率。

公开(公告)号：US20030209439A1

公开(公告)日：2003-11-13

申请号：US10427812

申请日：2003-05-01

Applicant: Alltech Associates, Inc.

Inventor： James M. Anderson JR. ,  Raaidah Saari-Nordhaus ,  Carl W. Sims ,  Yuri E. Gerner

IPC: G01N027/403

CPC classification number: G01N27/453 ,  B01D15/361 ,  B01D15/3885 ,  B01J39/26 ,  G01N30/02 ,  G01N30/34 ,  G01N30/468 ,  G01N30/52 ,  G01N30/96 ,  G01N2030/285 ,  G01N2030/965 ,  G01N2030/0035 ,  B01D15/362

Abstract: An apparatus and method for electrochemically modifying the retention of a species on a chromatography material is disclosed. The apparatus comprises a housing having an effluent flow channel adapted to permit fluid flow therethrough. The effluent flow channel comprises chromatography material. The apparatus further comprises first and second electrodes positioned such that at least a portion of the chromatography material is disposed between the first and second electrodes, and fluid flow through the apparatus is between, and in contact with, the first and second electrodes.

Abstract translation: 公开了用于电化学改变色谱材料上的物质保留的装置和方法。 该装置包括具有适于允许流体流过其中的流出物流动通道的壳体。 流出物流通道包括色谱材料。 该装置还包括第一和第二电极，其定位成使得至少一部分色谱材料设置在第一和第二电极之间，并且流过装置的流体在第一和第二电极之间并与之接触。

公开(公告)号：US06641708B1

公开(公告)日：2003-11-04

申请号：US09378874

申请日：1999-08-23

Applicant: Frederick F. Becker ,  Peter R. C. Gascoyne ,  Ying Huang ,  Xiao-Bo Wang ,  Jun Yang

Inventor： Frederick F. Becker ,  Peter R. C. Gascoyne ,  Ying Huang ,  Xiao-Bo Wang ,  Jun Yang

IPC: G01N2726

CPC classification number: B03C5/028 ,  B03C5/026 ,  G01N30/0005 ,  G01N2030/0035 ,  G01N2030/0065 ,  G01N2030/007

Abstract: Methods and apparatus for discriminating matter in a chamber having an inlet port and an outlet port utilizing dielectrophoresis and field flow fractionation. A carrier medium is introduced into the inlet port and is directed from the inlet port to the outlet port according to a velocity profile. A programmed voltage signal is applied to an electrode element coupled to the chamber to form a dielectrophoretic force on the matter. The dielectrophoretic force is balanced with a gravitational force to displace the matter to positions within said velocity profile in the carrier medium to discriminate the matter. A chamber having a top and bottom outlet port may be utilized to withdraw a first portion of a carrier medium from the top outlet port at a first, controllable fluid flow rate and to withdraw a second portion of the carrier medium from the bottom outlet port at a second, controllable fluid flow rate.

Abstract translation: 用于区分具有入口和出口的室中的物质的方法和装置，利用介电电泳和场流分级。 载体介质被引入入口并根据速度分布从入口端​​口引导到出口。 将编程的电压信号施加到耦合到腔室的电极元件以在该物质上形成介电电泳力。 介电电泳力与重力平衡以将物质移动到载体介质中的所述速度分布内的位置以区分该物质。 可以使用具有顶部和底部出口端口的腔室以第一可控流体流速从顶部出口端口抽出载体介质的第一部分，并且从底部出口端口将载体介质的第二部分从底部出口端口 第二可控流体流速。

公开(公告)号：US06613235B1

公开(公告)日：2003-09-02

申请号：US09561631

申请日：2000-05-02

Applicant: James M. Anderson, Jr. ,  Raaidah Saari-Nordhaus ,  Carl W. Sims ,  Yuri E. Gerner

Inventor： James M. Anderson, Jr. ,  Raaidah Saari-Nordhaus ,  Carl W. Sims ,  Yuri E. Gerner

IPC: G01N3002

CPC classification number: G01N27/453 ,  B01D15/361 ,  B01D15/3885 ,  B01J39/26 ,  G01N30/02 ,  G01N30/34 ,  G01N30/468 ,  G01N30/52 ,  G01N30/96 ,  G01N2030/285 ,  G01N2030/965 ,  G01N2030/0035 ,  B01D15/362

Abstract: An apparatus and method for electrochemically modifying the retention of a species on a chromatography material is disclosed. The apparatus comprises a housing having an effluent flow channel adapted to permit fluid flow therethrough. The effluent flow channel comprises chromatography material. The apparatus further comprises first and second electrodes positioned such that at least a portion of the chromatography material is disposed between the first and second electrodes, and fluid flow through the apparatus is between, and in contact with, the first and second electrodes.

Abstract translation: 公开了用于电化学改变色谱材料上的物质保留的装置和方法。 该装置包括具有适于允许流体流过其中的流出物流动通道的壳体。 流出物流通道包括色谱材料。 该装置还包括第一和第二电极，其定位成使得至少一部分色谱材料设置在第一和第二电极之间，并且流过装置的流体在第一和第二电极之间并与之接触。

公开(公告)号：US5888370A

公开(公告)日：1999-03-30

申请号：US605535

申请日：1996-02-23

Applicant: Frederick F. Becker ,  Peter R. C. Gascoyne ,  Ying Huang ,  Xiao-Bo Wang

Inventor： Frederick F. Becker ,  Peter R. C. Gascoyne ,  Ying Huang ,  Xiao-Bo Wang

IPC: B03C5/02 ,  G01N30/00 ,  G01N27/26

CPC classification number: G01N30/0005 ,  B03C5/028 ,  G01N2030/0035 ,  G01N2030/0065

Abstract: The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exits at the opposite end of the chamber at a characteristic position. Methods according to the invention involve using the apparatus for discriminating and separating matter for research, diagnosis of a condition, and therapeutic purposes. Examples of such methods may include separation of mixtures of cells, such as cancer cells from normal cells, separation of parasitized erythrocytes from normal erythrocytes, separation of nucleic acids, and others.

Abstract translation: 本公开涉及用于分离，表征和操纵物质的新型装置和新颖方法。 特别地，本发明结合了使用频率依赖的电介质和颗粒物质和溶解物质的导电性质与悬浮和输送介质的性质，以区分和分离这种物质。 该装置包括具有至少一个电极元件和至少一个入口和一个输出端口的腔室，细胞从腔室中被引入和移出。 然后通过流体流中的腔室进行的物质由通电电极引起的介电电泳（DEP）力在流体内移动。 在流体内移动之后，物质按照腔室的速度分布以速度行进通过腔室。 在物体经过腔室之后，它在特定位置处在腔室的相对端处离开。 根据本发明的方法涉及使用该装置来鉴别和分离用于研究，病症诊断和治疗目的的物质。 这些方法的实例可以包括分离细胞混合物，例如来自正常细胞的癌细胞，从正常红细胞分离寄生的红细胞，分离核酸等。

公开(公告)号：US3694335A

公开(公告)日：1972-09-26

申请号：US3694335D

申请日：1970-04-02

Applicant: PRETORIUS VICTOR ,  HANS HELMUTH HAHN

Inventor： PRETORIUS VICTOR ,  HAHN HANS HELMUTH

IPC: B01D11/02 ,  B01D11/04 ,  B01D15/00 ,  B01D15/08 ,  G01N27/447 ,  G01N27/49 ,  G01N30/02 ,  G01N30/06 ,  G01N30/14 ,  G01N30/32 ,  G01N30/34 ,  G01N30/38 ,  G01N30/42 ,  G01N30/46 ,  G01N30/50 ,  G01N30/52 ,  G01N30/56 ,  G01N30/62 ,  G01N30/64 ,  G01N30/84 ,  B01K5/00

CPC classification number: B01D69/10 ,  B01D11/0242 ,  B01D11/04 ,  B01D15/08 ,  B01D15/3885 ,  B01J2219/30207 ,  B01J2219/32206 ,  B01J2219/32213 ,  B01J2219/32227 ,  B01J2219/32231 ,  B01J2219/32286 ,  B01J2219/32296 ,  G01N27/404 ,  G01N27/447 ,  G01N30/02 ,  G01N30/06 ,  G01N30/14 ,  G01N30/34 ,  G01N30/38 ,  G01N30/42 ,  G01N30/50 ,  G01N30/56 ,  G01N30/64 ,  G01N30/84 ,  G01N2030/324 ,  G01N2030/387 ,  G01N2030/524 ,  G01N2030/528 ,  G01N2030/626 ,  G01N2030/645 ,  G01N2030/0035 ,  B01D15/36 ,  B01D15/361

Abstract: RETENTION OF SOLUTES ON THE RETARDING PHASE IS INDUCED BY APPLYING TO THE ENTIRE RETARDING PHASE AN ELECTRICAL POTENTIAL, ETHER UNIFORMLY OR WITH A GRADIENT. THE POTENTIAL MAY BE DC OR AC, IN THE LATTER CASE ADDITIONAL SEPARATION AEFFECTS ARE ATTAINABLE. THE AC PULSE SHAPE, FREQUENCY AND AMPLITUDE IS ADJUSTED WITH A PULSE GENERATOR.

公开(公告)号：US3594294A

公开(公告)日：1971-07-20

申请号：US3594294D

申请日：1966-10-03

Applicant: PRETORIUS VICTOR ,  HAHN HANS HELMUT

Inventor： PRETORIUS VICTOR ,  HAHN HANS HELMUT

IPC: G01N30/02 ,  B01D11/02 ,  B01D11/04 ,  B01D15/00 ,  B01D15/08 ,  B01D53/18 ,  G01N27/416 ,  G01N27/447 ,  G01N27/49 ,  G01N30/06 ,  G01N30/14 ,  G01N30/18 ,  G01N30/22 ,  G01N30/34 ,  G01N30/38 ,  G01N30/50 ,  G01N30/56 ,  G01N30/62 ,  G01N30/64 ,  G01N30/84 ,  B01K5/00

CPC classification number: B01D69/10 ,  B01D11/02 ,  B01D11/04 ,  B01D15/3885 ,  B01D53/18 ,  G01N27/447 ,  G01N30/06 ,  G01N30/14 ,  G01N30/34 ,  G01N30/38 ,  G01N30/50 ,  G01N30/56 ,  G01N30/64 ,  G01N30/84 ,  G01N2030/387 ,  G01N2030/626 ,  G01N2030/645 ,  G01N30/02 ,  G01N2030/0035 ,  B01D15/361

Abstract: FOR PURPOSES OF DETECTING CHANGES IN AN ELUATE IN CHROMATOGRAPHY A CELL COMPRISING A SOLID DETECTION ELECTRODE AND A REFERENCE ELECTRODE IS CONNECTED IN SERIES WITH A CHROMATOGRAPHIC SEPARATING SYSTEM, THE DETECTION ELECTRODE BEING IN DIRECT FLOWING CONTACT WITH THE ELUATE AND A LIQUID (WHICH MAY BE THE ELUATE ITSELF). A CONTROLLED POTENTIAL DIFFERENCE IS APPLIED TO THE ELECTRODES TO BRING ABOUT A CHEMICAL OXIDATION/REDUCTION REACTION OF THE SUBSTANCES BEING SEPARATED AND THE RELATIONSHIP BETWEEN AT LEAST TWO QUANTITIES INTER-RELATED BY OHM''S LAW IS OBSERVED. PROVISION IS MADE FOR A COMPENSATORY CELL AND FOR THE ELECTRO-CHEMICAL REMOVAL FROM THE ELUANT OF IMPURITIES WHICH INTERFERE WITH THE DETECTION. A PREFERRED CELL IS TUBULAR WITH THE ELECTRODES E.G. OF GRAPHITE OR GLASSY CARBON PROVIDED BY THE WALLS OF THE CELL OR EXTENDING ACROSS THE CELL.

D R A W I N G

公开(公告)号：US07918980B2

公开(公告)日：2011-04-05

申请号：US11397906

申请日：2006-04-05

Applicant: Victor Morozov

Inventor： Victor Morozov

IPC: G01N27/453

CPC classification number: G01N33/5438 ,  G01N27/44769 ,  G01N2030/0035

Abstract: A flow cell for electrophoretically-assisted capturing analytes from a flow. The flow cell includes a specimen chamber, a first membrane, a second membrane, a first electrode chamber, and a second electrode chamber. The specimen chamber may have a sample inlet and a sample outlet. A first portion of the first membrane may be coupled to a first portion of the specimen chamber. A first portion of the second membrane may be coupled to a second portion of the specimen chamber. The first electrode chamber may be configured to accept a charge. A portion of the first electrode chamber may be coupled to a second portion of the first membrane. A second electrode chamber may be configured to accept an opposite charge. A portion of the second electrode chamber may be coupled to a second portion of the second membrane.

Abstract translation: 用于电泳辅助从流中捕获分析物的流动池。 流动池包括试样室，第一膜，第二膜，第一电极室和第二电极室。 样品室可以具有样品入口和样品出口。 第一膜的第一部分可以联接到样品室的第一部分。 第二膜的第一部分可以联接到样品室的第二部分。 第一电极室可以被配置为接受电荷。 第一电极室的一部分可以耦合到第一膜的第二部分。 第二电极室可以被配置为接受相反的电荷。 第二电极室的一部分可以联接到第二膜的第二部分。