公开(公告)号：US20050224134A1

公开(公告)日：2005-10-13

申请号：US10821543

申请日：2004-04-09

申请人： Hongfeng Yin ,  Kevin Killeen ,  Reid Brennen ,  Uwe Effelsberg

发明人： Hongfeng Yin ,  Kevin Killeen ,  Reid Brennen ,  Uwe Effelsberg

IPC分类号： G01N30/56 ,  G01N35/00 ,  B67C3/02

CPC分类号： G01N30/56 ,  B01J2219/00466 ,  G01N2030/565 ,  G01N2035/00574

摘要： Provided are apparatuses and methods for introducing particles into a microdevice conduit. Also provided are microdevices containing a plurality of particles that occupy at least about 25 volume percent of a microconduit. In some instances, particles may controllably form a particle bridge in a bridging zone within a microdevice.

摘要翻译： 提供了将颗粒引入微型设备导管中的装置和方法。 还提供了包含占据至少约25体积％微管道的多个颗粒的微器件。 在一些情况下，颗粒可以可控制地在微设备内的桥接区域中形成颗粒桥。

公开(公告)号：US06845968B2

公开(公告)日：2005-01-25

申请号：US10785336

申请日：2004-02-23

申请人： Kevin Killeen ,  Hongfeng Yin

发明人： Kevin Killeen ,  Hongfeng Yin

IPC分类号： F15C5/00 ,  F16K99/00 ,  F16K31/02

CPC分类号： F16K99/0001 ,  F15C5/00 ,  F16K99/0011 ,  F16K99/0013 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T137/86863

摘要： A microdevice is provided for controlling fluid flow. The microdevice includes a substantially planar contact surface and a plurality of fluid-transporting features associated therewith. Also included is a substrate having a substantially planar contact surface and a fluid-transporting feature associated therewith. The contact surfaces are positioned in slidable and fluid-tight contact to allow for controllable formation of a plurality of different flow paths upon alignment of the substrate fluid-transporting feature with each cover plate fluid-transporting features in succession. Typically, at least one of the cover plate and substrate is comprised of a biofouling resistant polymer, and the flow paths are of different lengths. Optionally, a plurality of fluid-transporting features is associated with the substrate so that flow paths are formed as a result of a different alignment of the fluid-transporting features.

摘要翻译： 提供微型装置用于控制流体流动。 微型装置包括基本上平面的接触表面和与其相关联的多个流体输送特征。 还包括具有基本上平面的接触表面和与其相关联的流体输送特征的基底。 接触表面定位在可滑动和流体密封的接触中，以允许在衬底流体输送特征与每个盖板流体输送特征相继对准时可控地形成多个不同的流动路径。 通常，盖板和衬底中的至少一个由耐生物结垢的聚合物组成，并且流动路径具有不同的长度。 可选地，多个流体输送特征与基底相关联，使得由于流体输送特征的不同对准而形成流动路径。

公开(公告)号：US06702256B2

公开(公告)日：2004-03-09

申请号：US09908292

申请日：2001-07-17

申请人： Kevin Killeen ,  Hongfeng Yin

发明人： Kevin Killeen ,  Hongfeng Yin

IPC分类号： F16K500

CPC分类号： F16K99/0001 ,  F15C5/00 ,  F16K99/0011 ,  F16K99/0013 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T137/86863

摘要： The present invention relates to a microdevice having a valve structure that contains low or no dead volume. The microdevice comprises a substrate and a cover plate each having a substantially planar contact surface and each contact surface having a fluid-transporting feature associated therewith. The substrate contact surface is positioned in slidable and fluid-tight contact with the cover plate contact surface to allow for controllable alignment between the fluid-transporting features. As a result, fluid communication is provided therebetween through a small area. Where the cover plate fluid-transporting feature comprises a conduit, the conduit may have a substantially constant cross-sectional area. The controllable alignment between the fluid-transporting features may form an alignment-dependent variable-length flow path.

摘要翻译： 本发明涉及具有低或非死体积的阀结构的微型装置。 微型装置包括基板和盖板，每个底板具有基本上平面的接触表面，并且每个接触表面具有与其相关联的流体输送特征。 衬底接触表面定位成与盖板接触表面可滑动和流体密封地接触，以允许流体输送特征之间的可控制的对准。 结果，通过小区域在它们之间提供流体连通。 在盖板流体输送特征包括导管的情况下，导管可具有基本恒定的横截面积。 流体输送特征之间的可控对准可以形成取决于可变长度的流动路径。

公开(公告)号：US06462337B1

公开(公告)日：2002-10-08

申请号：US09552962

申请日：2000-04-20

申请人： Gangqiang Li ,  Hongfeng Yin

发明人： Gangqiang Li ,  Hongfeng Yin

IPC分类号： H01J4910

CPC分类号： H01J49/165 ,  C07D417/12 ,  C12P17/14 ,  C12P17/16

摘要： A method of providing ions to a mass spectrometer having an interface member with an orifice through which ions are received for analysis. A fluid is provided at a capillary tip of an electrospray ionization source and a potential difference applied between the tip and the interface member so as to direct an ion beam from the tip toward and through the orifice. A field enhancing potential is applied at an auxiliary electrode so as to increase the electric field gradient from the capillary tip at least part way toward the interface member. A focussing potential may be applied to the auxiliary electrode to generate an electric field which decreases beam divergence from at least part way toward the interface member. Both the field enhancing and focusing potential may alternately be applied to the auxiliary electrode.

摘要翻译： 向质谱仪提供离子的方法，所述质谱仪具有接口部件，所述接口部件具有接口用于分析的离子。 在电喷雾电离源的毛细管尖端处设置有流体，并且在尖端和界面部件之间施加电位差，以便将尖端的离子束引导并穿过孔口。 在辅助电极处施加场强增强电位，以便使毛细管尖端的电场梯度至少部分地向接口部件增加。 可以将辅助电极施加聚焦电位以产生电场，该电场将波束发散从至少部分地朝向接口部件减小。 场增强和聚焦电位都可以替代地施加到辅助电极。

公开(公告)号：US06386050B1

公开(公告)日：2002-05-14

申请号：US09468695

申请日：1999-12-21

申请人： Hongfeng Yin ,  Catherine Templin

发明人： Hongfeng Yin ,  Catherine Templin

IPC分类号： G01F1708

CPC分类号： A61M5/16886 ,  A61M2205/3306 ,  G01F1/6847 ,  G01F1/7084 ,  G01F1/7086 ,  G01F1/7088

摘要： A system and method for measuring flow rate within a fluid-bearing passageway include introducing heat fluctuations into the flow and then non-invasively monitoring the effects of the heat fluctuations propagating to or from one or more interrogation regions. In one embodiment, the non-invasive monitoring detects fluctuations in the refractive index of the flowing fluid as a result of variations in the temperature of the fluid. In another embodiment, electrical conductivity of the fluid is monitored. The heat fluctuations may be introduced using an optical heat generator, such as an infrared laser, or may be introduced using an electrical member, such as a heater coil. Determining the refractive index along the interrogation region may be achieved by monitoring characteristics on an interference pattern, but other optical arrangements may be utilized.

摘要翻译： 用于测量流体承载通道内的流量的系统和方法包括将热波动引入流中，然后非侵入性地监测传播到或来自一个或多个询问区域的热波动的影响。 在一个实施例中，非侵入性监测由于流体温度的变化而检测流动流体的折射率的波动。 在另一个实施例中，监测流体的导电性。 可以使用诸如红外激光器的光学热发生器引入热波动，或者可以使用诸如加热器线圈的电气部件来引入热波动。 沿着询问区域确定折射率可以通过监测干涉图案上的特性来实现，但也可以利用其他光学布置。

公开(公告)号：US06375901B1

公开(公告)日：2002-04-23

申请号：US09294867

申请日：1999-04-20

申请人： Karla M. Robotti ,  Sally A. Swedberg ,  Hongfeng Yin

发明人： Karla M. Robotti ,  Sally A. Swedberg ,  Hongfeng Yin

IPC分类号： B01L1100

CPC分类号： B81B3/0035 ,  B01J19/0093 ,  B01J2219/00891 ,  B01J2219/00907 ,  B01J2219/00909 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0025 ,  F16K99/0036 ,  F16K99/0044 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0078 ,  F16K2099/0084 ,  Y10T137/2082 ,  Y10T436/2575

摘要： Micro-fluid devices and methods for their use are provided. The subject devices are characterized by the presence of at least one micro-valve comprising a phase reversible material, e.g. a reversible gel, that reversibly changes its physical state in response to an applied stimulus, e.g. a thermoreversible gel. In using the subject device, fluid flow along a flow path of the device is modulated by applying an appropriate stimulus, e.g. changing the temperature, to the microvalve. The subject devices find use in a variety of applications, including micro-analytical applications.

公开(公告)号：US5650846A

公开(公告)日：1997-07-22

申请号：US562082

申请日：1995-11-21

申请人： Hongfeng Yin ,  Catherine Keely Templin

发明人： Hongfeng Yin ,  Catherine Keely Templin

IPC分类号： G01N21/64 ,  G01N21/01 ,  G01N21/05 ,  G01N21/65 ,  G01N27/447 ,  G01N30/74

CPC分类号： G01N21/645 ,  G01N27/44721 ,  G01N30/74 ,  G01N2021/6467 ,  G01N2021/6482 ,  G01N2021/6484

摘要： An analytical system for analyzing a fluid sample is disclosed. The analytical system includes a microcolumn for conducting the fluid sample, a light source for delivering light through the microcolumnar wall into the microcolumn near the outlet end of that microcolumn, and an optical fiber aligned with the microcolumn to detect light that radiates from the fluid sample without passing through the microcolumnar wall. The microcolumn has an inlet end and an outlet end. A light-inlet end of the optical fiber is nonfixedly coupled to the outlet end of the microcolumn. As the fluid sample is driven from the inlet end to the outlet end of the microcolumn, a light of a suitable wavelength is directed at the microcolumn near its outlet end to cause light interaction with the fluid sample. As a result, light radiates from the fluid sample. This light is collected by the optical fiber to provide information on the presence or quantity of an analyte in the fluid sample.

摘要翻译： 公开了一种用于分析流体样品的分析系统。 分析系统包括用于导入流体样品的微柱，用于将光通过微柱壁输送到微柱出口端附近的微柱中的光源和与微柱对准的光纤以检测从流体样品辐射的光 而不通过微柱壁。 微柱有入口端和出口端。 光纤的光入口端非固定地耦合到微柱的出口端。 当流体样品从微柱的入口端到出口端被驱动时，适当波长的光指向靠近其出口端的微柱，以引起与流体样品的光相互作用。 结果，光从流体样品辐射。 该光被光纤收集以提供关于流体样品中分析物的存在或数量的信息。

公开(公告)号：US08504575B2

公开(公告)日：2013-08-06

申请号：US11394343

申请日：2006-03-29

申请人： Joshua M. Koran ,  Christina Yip Chung ,  Abhinav Gupta ,  George H. John ,  Hongfeng Yin ,  Long-Ji Lin ,  Richard Frankel

发明人： Joshua M. Koran ,  Christina Yip Chung ,  Abhinav Gupta ,  George H. John ,  Hongfeng Yin ,  Long-Ji Lin ,  Richard Frankel

IPC分类号： G06F17/30

CPC分类号： G06Q30/0255 ,  G06F17/3053 ,  G06F17/30702 ,  G06F17/30761 ,  G06F17/30914 ,  G06N5/02 ,  G06Q10/067 ,  G06Q30/02

摘要： A behavioral targeting system determines user profiles from online activity. The system includes a plurality of models that define parameters for determining a user profile score. Event information, which comprises on-line activity of the user, is received at an entity. To generate a user profile score, a model is selected. The model comprises recency, intensity and frequency dimension parameters. The behavioral targeting system generates a user profile score for a target objective, such as brand advertising or direct response advertising. The parameters from the model are applied to generate the user profile score in a category. The behavioral targeting system has application for use in ad serving to on-line users.

摘要翻译： 行为定位系统从在线活动确定用户个人资料。 该系统包括多个模型，其定义用于确定用户简档分数的参数。 事件信息，包括用户的在线活动，在一个实体被接收。 要生成用户配置文件分数，选择一个模型。 该模型包括新近度，强度和频率尺寸参数。 行为定位系统为目标目标生成用户简档分数，例如品牌广告或直接响应广告。 应用来自模型的参数以在类别中生成用户简档分数。 行为定位系统具有在在线用户的广告投放中使用的应用。

公开(公告)号：US20110161331A1

公开(公告)日：2011-06-30

申请号：US13010605

申请日：2011-01-20

申请人： Christina Yip Chung ,  Abbinav Gupta ,  Joshua M. Koran ,  Long-Ji Lin ,  Hongfeng Yin

发明人： Christina Yip Chung ,  Abbinav Gupta ,  Joshua M. Koran ,  Long-Ji Lin ,  Hongfeng Yin

IPC分类号： G06F17/30

CPC分类号： G06Q30/02

摘要： A behavioral targeting system determines user profiles from online activity. The system includes a plurality of models that define parameters for determining a user profile score. Event information, which comprises on-line activity of the user, is received at an entity. To generate a user profile score, a model is selected. The model comprises recency, intensity and frequency dimension parameters. The behavioral targeting system generates a user profile score for a target objective, such as brand advertising or direct response advertising. The parameters from the model are applied to generate the user profile score in a category. The behavioral targeting system has application for use in ad serving to on-line users.

摘要翻译： 行为定位系统从在线活动确定用户个人资料。 该系统包括多个模型，其定义用于确定用户简档分数的参数。 事件信息，包括用户的在线活动，在一个实体被接收。 要生成用户配置文件分数，选择一个模型。 该模型包括近似度，强度和频率尺寸参数。 行为定位系统为目标目标生成用户简档分数，例如品牌广告或直接响应广告。 应用来自模型的参数以在类别中生成用户简档分数。 行为定位系统具有在在线用户的广告投放中使用的应用。

公开(公告)号：US07939798B2

公开(公告)日：2011-05-10

申请号：US12363685

申请日：2009-01-30

申请人： Gangqiang Li ,  Hongfeng Yin

发明人： Gangqiang Li ,  Hongfeng Yin

IPC分类号： H01J49/10

CPC分类号： H01J49/107 ,  H01J49/165

摘要： An ion source a first ionizer comprising: an electrospray needle comprising a tip; and a conduit disposed annularly about the needle and configured to pass an inert gas in proximity of the tip to nebulize a fluid emerging from the tip, the nebulized fluid comprising analytes and a mobile phase. The ion source comprises a capillary in tandem with the first ionizer and configured to receive the droplets; a heater configured to heat the capillary to a temperature at which mobile phase vaporizes; and a second ionizer in tandem with the capillary and configured to receive the vaporized mobile phase and the analytes. A method is also described.

摘要翻译： 离子源，第一离子发生器，包括：电喷针，包括尖端; 以及围绕所述针环设置的导管，其被配置成使所述尖端附近的惰性气体通过，以使从所述尖端出来的流体雾化，所述雾化流体包含分析物和流动相。 离子源包括与第一离子发生器串联的毛细管并且构造成接收液滴; 加热器，被配置为将毛细管加热至流动相蒸发的温度; 以及第二离子发生器，其与所述毛细管串联并且被配置为接收蒸发的流动相和所述分析物。 还描述了一种方法。