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公开(公告)号：US20150141263A1
公开(公告)日：2015-05-21
申请号：US14411628
申请日：2013-06-28
Applicant: Massachusetts Institute of Technology
Inventor： Timothy Kuan-Ta Lu , Allen Cheng
CPC classification number: C12N15/1065 , C12N15/1027 , C12N15/1079 , C12N15/66 , C12Q1/6869 , G01N2035/00752 , C12Q2525/131 , C12Q2563/179
Abstract: The invention relates to methods and compositions that enable the rapid generation of high-order combinations of genetic elements, and that provide a barcoded basis for rapid characterization of the specific combination of genetic elements encoded within a single cell or in a pooled population.
Abstract translation: 本发明涉及能够快速产生遗传元件的高阶组合的方法和组合物，并且提供了用于快速表征在单个细胞或合并的群体内编码的遗传元件的特定组合的条形码基础的方法和组合物。
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52.发明申请ENGINEERED ENZYMATICALLY ACTIVE BACTERIOPHAGE AND METHODS FOR DISPERSING BIOFILMS 审中-公开工程化生物活性细菌和分散生物膜的方法公开(公告)号：US20140161772A1
公开(公告)日：2014-06-12
申请号：US13953114
申请日：2013-07-29
Inventor： James J. Collins , Timothy Kuan-Ta Lu
IPC: A01N63/02
CPC classification number: A01N63/02 , C12N7/00 , C12N9/2402 , C12N2795/10211 , C12N2795/10221 , C12N2795/10232 , C12Y207/07006 , C12Y302/01052
Abstract: The present invention is directed to engineered enzymatically active bacteriophages that are both capable of killing the bacteria by lysis and dispersing the bacterial biofilm because they have been also engineered to express biofilm-degrading enzymes, particularly dispersin B (DspB), an enzyme that hydrolyzes β-1,6-N-acetyl-D-glucosamine, a crucial adhesion molecule needed for biofilm formation and integrity in Staphylococcus and E. coli, including E. coli K-12, as well as clinical isolates.
Abstract translation: 本发明涉及工程化的酶活性噬菌体，其能够通过裂解和分散细菌生物膜来杀死细菌，因为它们也被工程化以表达生物降膜酶，特别是分解B（DspB），其是水解＆bgr的酶 ; -1,6-N-乙酰基-D-葡糖胺，生物膜形成所需的关键粘附分子和葡萄球菌和大肠杆菌（包括大肠杆菌K-12）以及临床分离物中的完整性。
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公开(公告)号：US20130053432A1
公开(公告)日：2013-02-28
申请号：US13661169
申请日：2012-10-26
Inventor： James J. Collins , Timothy Kuan-Ta Lu
CPC classification number: C12N15/1086 , C12N15/111 , C12N15/63 , C12N2310/111 , C12N2320/50
Abstract: Described are methods and compositions for the detection of target genes. The inventors have developed a synthetic nucleic acid sensor-effector gene circuit. In cells without a target gene, the circuit suppresses e.g., effector production, but in the presence of the target gene the suppression is subject to competition, such that the synthetic sensor is de-repressed and permits expression of the effector gene. The methods and compositions described further permit the selective expression of an effector gene in those cells expressing the target gene. In this manner, cells expressing a target gene can be selectively targeted for treatment or elimination. In certain aspects, the methods and compositions described permit the selective expression of an agent such as a therapeutic gene product, in a specifically targeted population of cells in an organism.
Abstract translation: 描述了用于检测靶基因的方法和组合物。 本发明人开发了合成核酸传感器 - 效应子基因电路。 在没有靶基因的细胞中，电路抑制例如效应子产生，但是在靶基因的存在下，抑制受到竞争，使得合成传感器被去抑制并且允许效应基因的表达。 所描述的方法和组合物进一步允许在表达靶基因的那些细胞中选择性表达效应基因。 以这种方式，表达靶基因的细胞可以选择性地靶向用于治疗或消除。 在某些方面，所描述的方法和组合允许在生物体中的特定靶细胞群中选择性地表达诸如治疗性基因产物的试剂。
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公开(公告)号：US11875876B2
公开(公告)日：2024-01-16
申请号：US16441647
申请日：2019-06-14
Applicant: Massachusetts Institute of Technology
Inventor： Timothy Kuan-Ta Lu , Manolis Kellis , Jicong Cao , Eva Maria Novoa Pardo , Zhizhuo Zhang
CPC classification number: G16B20/50 , C12N15/1089 , C12N15/11 , C12N15/85 , G16B40/00
Abstract: Disclosed herein are novel 5′ UTR sequences that can be used to control gene expression in various contexts. Also disclosed herein are methods of engineering 5′ UTR sequences and methods and kits for screening 5′ UTR sequences for a property of interest.
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公开(公告)号：US11850290B2
公开(公告)日：2023-12-26
申请号：US16318958
申请日：2017-07-20
Applicant: Massachusetts Institute of Technology
Inventor： Timothy Kuan-Ta Lu , Xinyue Liu , Tzu-Chieh Tang , Eléonore Claire Tham , Xuanhe Zhao
CPC classification number: A61K49/0097 , A61B5/6806 , A61B5/6833 , A61K49/0073 , C12N11/04 , C12N11/10 , C12N15/63 , C12N15/70 , C12Q1/6897
Abstract: Provided herein, in some embodiments, are hydrogel-elastomer and hydrogel-alginate devices, compositions and associated methods to encapsulate living cells.
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Patent Agency Ranking
公开(公告)号：US20220396762A1
公开(公告)日：2022-12-15
申请号：US17636259
申请日：2020-08-21
Inventor： Timothy Kuan-Ta Lu , Tzu-Chieh Tang , Wolfgang Ott , Tom Ellis , Charles Gilbert
Abstract: The disclosure provides compositions and methods for growing programmable enzyme-functionalized and sense-and-response bacterial cellulose living materials with engineered microbial co-cultures.
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公开(公告)号：US20220378363A1
公开(公告)日：2022-12-01
申请号：US17730075
申请日：2022-04-26
Applicant: Massachusetts Institute of Technology , Trustees of Boston University , The Brigham and Women's Hospital, Inc.
Inventor： Timothy Kuan-Ta Lu , Rabia Tugce Yazicigil Kirby , Carlo Giovanni Traverso , Jenna Ahn , Maria Eugenia Inda , Miguel Jimenez , Qijun Liu , Phillip Nadeau , Christoph Winfried Johannes Steiger , Adam Wentworth
IPC: A61B5/00 , C12Q1/6897 , A61B5/145 , A61B5/1459
Abstract: Transient molecules in the gastrointestinal (GI) tract, such as nitric oxide and hydrogen sulfide, are important signals and mediators of inflammatory bowel disease (IBD). Because these molecules may be short-lived in the body, they are difficult to detect. To track these reactive molecules in the GI tract, a miniaturized device has been developed that integrates genetically engineered probiotic biosensors with a custom-designed photodetector and readout chip. Leveraging the molecular specificity of living sensors, bacteria were genetically encoded to respond to IBD-associated molecules by luminescing. Low-power electronic readout circuits (e.g., using nanowatt power) integrated into the device convert the light from just 1 μL of bacterial culture into a wireless signal. Biosensor monitoring was demonstrated in the GI tract of small and large animal models and integration of all components into a sub-1.4 cm3 ingestible form factor capable of supporting wireless communication. The wireless detection of short-lived, disease-associated molecules may support earlier diagnosis of disease than is currently possible, more accurate tracking of disease progression, and more timely communication between patient and their care team supporting remote personalized care.
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公开(公告)号：US20210310022A1
公开(公告)日：2021-10-07
申请号：US17338027
申请日：2021-06-03
Applicant: Massachusetts Institute of Technology
Inventor： Timothy Kuan-Ta Lu , Alan Siu Lun Wong , Ching Gee Choi
IPC: C12N15/85 , C12N15/10 , A61K31/4709 , A61K31/55
Abstract: Described herein are methods and compositions that enable rapid generation of high-order combinations of genetic elements comprising a CRISPR guide sequence and a scaffold sequence, and a barcode for rapid identification of the combination of genetic elements encoded within a single cell or a pooled population. Also described herein compositions of inhibitors of epigenetic genes and methods for reducing cell proliferation and/or treating cancer.
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公开(公告)号：US11091762B2
公开(公告)日：2021-08-17
申请号：US16815072
申请日：2020-03-11
Applicant: Massachusetts Institute of Technology
Inventor： Timothy Kuan-Ta Lu , Sebastien Lemire , Andrew C. Yang , Kevin M. Yehl
IPC: C12N9/22 , C12Q1/68 , C12N15/113 , C12N15/74 , C12N15/70 , G01N33/569 , A61P31/04 , C07K14/005
Abstract: Disclosed herein are novel synthetic bacteriophages and bacteriophage compositions, methods of production thereof, and therapeutic uses thereof.
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公开(公告)号：US10968454B2
公开(公告)日：2021-04-06
申请号：US15924045
申请日：2018-03-16
Inventor： Timothy Kuan-Ta Lu , Robert James Citorik , James Collins , Russell-John Krom
Abstract: Various aspects and embodiments of the present disclosure are directed to methods and compositions for functionalizing endogenous bacteria in vivo. The methods include delivering to endogenous bacterial cells a recombinant bacteriophage or phagemid that is engineered to contain at least one genetic circuit.
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