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74 条记录 (耗时 0.02 秒)

    DNA-based integrated circuit
    51.
    发明申请
    DNA-based integrated circuit 失效

    公开(公告)号：US20020042151A1

    公开(公告)日：2002-04-11

    申请号：US09964745

    申请日：2001-09-28

    发明人： Boris Chen

    IPC分类号： H01L021/00 ,  C12Q001/68

    CPC分类号： H01L51/0093 ,  B82Y10/00 ,  H01L27/28 ,  H01L51/0508 ,  H01L51/0595 ,  Y10S977/704 ,  Y10S977/728 ,  Y10S977/80 ,  Y10S977/887

    摘要： This Invention, a kind of DNA-based integrated circuit, with the DNA dyeing technology, the inlaying principle between anti-cancer medicine and DNA molecules, can change energy gap of DNA molecules to alter the conductivity of DNA molecules. Because that the diameter of DNA molecules is only about 2 nm, this kind of electronic element, which is not made with photolithography technologies, not only avoids the bottleneck of line width in production of photolithography-based ICs, but also limits the line width to 2 nm, much less than the minimum line width (0.13 nullm or 130 nm) in semi-conductor production industry. It brings a practical approach to IC design beyond photolithography technologies, and ensures the development of ICs to micro-miniature predicted by the Moore Law.

    Nucleic acids encoding self-assembled, non-infectious, non-replicating, immunogenic retrovirus-like particles comprising modified HIV genomes and chimeric envelope glycoproteins
    53.
    发明授权
    Nucleic acids encoding self-assembled, non-infectious, non-replicating, immunogenic retrovirus-like particles comprising modified HIV genomes and chimeric envelope glycoproteins 失效
    编码自组装，非感染，非复制，免疫原性逆转录病毒样颗粒的核酸，其包含修饰的HIV基因组和嵌合包膜糖蛋白

    公开(公告)号：US5912338A

    公开(公告)日：1999-06-15

    申请号：US452520

    申请日：1995-05-30

    申请人： Benjamin Rovinski ,  Joel Haynes ,  Shi Xian Cao ,  Michel Henri Klein

    发明人： Benjamin Rovinski ,  Joel Haynes ,  Shi Xian Cao ,  Michel Henri Klein

    IPC分类号： C07K16/10 ,  G01N33/569 ,  C07H21/04

    CPC分类号： G01N33/56988 ,  C07K16/1063 ,  A61K2039/5258 ,  Y10S435/81 ,  Y10S435/975 ,  Y10S977/759 ,  Y10S977/80 ,  Y10S977/802 ,  Y10S977/804 ,  Y10S977/915 ,  Y10S977/916 ,  Y10S977/917 ,  Y10S977/918 ,  Y10S977/92

    摘要： This invention is directed toward nucleic acids encoding self-assembled, non-infectious, non-replicating, immunogenic retrovirus-like particles comprising modified HIV-1 genomes devoid of long terminal repeats and containing nucleotide sequences encoding chimeric envelope glycoproteins. Retrovirus-like particles containing chimeric envelope glycoproteins were expressed in mammalian cells by using inducible promoters. One preferred embodiment discloses the engineering of a series of expression vectors in which a synthetic oligomer encoding gp120 residues 306 to 328 (amino acids YNKRKRIHIGP GRAFYTTKNIIG) from the V3 loop of the MN viral isolate was inserted at various positions within the endogenous HIV-1.sub.LAI env gene. Expression studies revealed that insertion of the heterologous V3(MN) loop segment resulted in the secretion of fully assembled HIV-like particles containing chimeric LAI/MN envelope glycoproteins. Both V3 loop epitopes were recognized by loop-specific neutralizing antibodies. Immunization with HIV-like particles containing chimeric envelope proteins induced specific antibody responses against both the autologous and heterologous V3 loop epitopes, including cross-neutralizing antibodies against the HIV-1.sub.LAI and HIV-1.sub.MN isolates.

    摘要翻译： 本发明涉及编码自组装，非感染性，非复制性，免疫原性逆转录病毒样颗粒的核酸，其包含缺乏长末端重复并且含有编码嵌合包膜糖蛋白的核苷酸序列的修饰的HIV-1基因组。 含有嵌合包膜糖蛋白的逆转录病毒样颗粒通过使用诱导型启动子在哺乳动物细胞中表达。 一个优选实施方案公开了一系列表达载体的工程，其中编码来自MN病毒分离物的V3环的gp120残基306至328（氨基酸YNKRKRIHIGP GRAFYTTKNIIG）的合成低聚物插入内源性HIV-1LAI环内的各个位置 基因。 表达研究显示异源V3（MN）环片段的插入导致完整组装的含有嵌合LAI / MN包膜糖蛋白的HIV样颗粒的分泌。 通过环特异性中和抗体识别出两个V3环表位。 含有嵌合包膜蛋白的HIV样颗粒的免疫诱导针对自体和异源V3环表位的特异性抗体应答，包括针对HIV-1LAI和HIV-1MN分离株的交叉中和抗体。

    Self-assembled, non-infectious, non-replicating; immunogenic retrovirus-like particles comprising modified HIV genomes devoid of long terminal repeats and chimeric envelope glycoproteins
    54.
    发明授权
    Self-assembled, non-infectious, non-replicating; immunogenic retrovirus-like particles comprising modified HIV genomes devoid of long terminal repeats and chimeric envelope glycoproteins 失效
    自组装，非传染性，非复制性; 包含缺乏长末端重复的修饰的HIV基因组和嵌合包膜糖蛋白的免疫原性逆转录病毒样颗粒

    公开(公告)号：US5866137A

    公开(公告)日：1999-02-02

    申请号：US453745

    申请日：1995-05-30

    申请人： Benjamin Rovinski ,  Joel Haynes ,  Shi Xian Cao ,  Michel Henri Klein

    发明人： Benjamin Rovinski ,  Joel Haynes ,  Shi Xian Cao ,  Michel Henri Klein

    IPC分类号： C07K16/10 ,  G01N33/569 ,  A61K39/12 ,  A61K39/21 ,  C12N7/00 ,  C12N7/01

    CPC分类号： G01N33/56988 ,  C07K16/1063 ,  A61K2039/5258 ,  Y10S435/81 ,  Y10S435/975 ,  Y10S977/759 ,  Y10S977/80 ,  Y10S977/802 ,  Y10S977/804 ,  Y10S977/915 ,  Y10S977/916 ,  Y10S977/917 ,  Y10S977/918 ,  Y10S977/92

    摘要： This invention is directed toward self-assembled, non-infectious, non-replicating, immunogenic retrovirus-like particles comprising modified HIV-1 genomes devoid of long terminal repeats and containing nucleotide sequences encoding chimeric envelope glycoproteins. Retrovirus-like particles containing chimeric envelope glycoproteins were expressed in mammalian cells by using inducible promoters. One preferred embodiment discloses the engineering of a series of expression vectors in which a synthetic oligomer encoding gp120 residues 306 to 328 (amino acids YNKRKRIHIGP GRAFYTTKNIIG) from the V3 loop of the MN viral isolate was inserted at various positions within the endogenous HIV-1.sub.LAI env gene. Expression studies revealed that insertion of the heterologous V3(MN) loop segment resulted in the secretion of fully assembled HIV-like particles containing chimeric LAI/MN envelope glycoproteins. Both V3 loop epitopes were recognized by loop-specific neutralizing antibodies. Immunization with HIV-like particles containing chimeric envelope proteins induced specific antibody responses against both the autologous and heterologous V3 loop epitopes, including cross-neutralizing antibodies against the HIV-1.sub.LAI and HIV-1.sub.MN isolates.

    摘要翻译： 本发明涉及自组装的非感染性非复制性免疫原性逆转录病毒样颗粒，其包含缺乏长末端重复并且含有编码嵌合包膜糖蛋白的核苷酸序列的经修饰的HIV-1基因组。 含有嵌合包膜糖蛋白的逆转录病毒样颗粒通过使用诱导型启动子在哺乳动物细胞中表达。 一个优选实施方案公开了一系列表达载体的工程，其中编码来自MN病毒分离物的V3环的gp120残基306至328（氨基酸YNKRKRIHIGP GRAFYTTKNIIG）的合成低聚物插入内源性HIV-1LAI环内的各个位置 基因。 表达研究显示异源V3（MN）环片段的插入导致完整组装的含有嵌合LAI / MN包膜糖蛋白的HIV样颗粒的分泌。 通过环特异性中和抗体识别出两个V3环表位。 含有嵌合包膜蛋白的HIV样颗粒的免疫诱导针对自体和异源V3环表位的特异性抗体应答，包括针对HIV-1LAI和HIV-1MN分离株的交叉中和抗体。