公开(公告)号：US20070054273A1

公开(公告)日：2007-03-08

申请号：US10566329

申请日：2003-08-13

Applicant: Feijun Xian ,  Wanli Xing ,  Jing Cheng ,  Dong Liang ,  Liang Zhang ,  Dong Wang

Inventor： Feijun Xian ,  Wanli Xing ,  Jing Cheng ,  Dong Liang ,  Liang Zhang ,  Dong Wang

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: B01L3/50853 ,  B01J2219/00281 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01L9/52 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0822 ,  C12Q1/6837 ,  C40B60/14 ,  G01N33/54366

Abstract: This invention relates generally to the field of microarray chips and uses thereof. In particular, the invention provides a microarray reaction device that can be used in assaying the interaction between various moieties, e.g., nucleic acids, immunoreactions involving proteins, interactions between a protein and a nucleic acid, a ligand-receptor interaction, and small molecule and protein or nucleic acid interactions, etc. Articles of manufacture and kits comprising the microarray reaction device and assaying methods using the microarray reaction device are also provided.

Abstract translation: 本发明一般涉及微阵列芯片领域及其用途。 特别地，本发明提供一种微阵列反应装置，其可用于测定各种部分之间的相互作用，例如核酸，涉及蛋白质的免疫反应，蛋白质与核酸之间的相互作用，配体 - 受体相互作用和小分子，以及 蛋白质或核酸相互作用等。还提供了包含微阵列反应装置的生产制品和试剂盒以及使用微阵列反应装置的测定方法。

公开(公告)号：US07138041B2

公开(公告)日：2006-11-21

申请号：US10784681

申请日：2004-02-23

Applicant: Chein-Shyong Su ,  Chia-Wei Chang ,  Miao-Ling Hung ,  Wen-Jing Cheng ,  Tai-Guang Wu

Inventor： Chein-Shyong Su ,  Chia-Wei Chang ,  Miao-Ling Hung ,  Wen-Jing Cheng ,  Tai-Guang Wu

IPC: G01N27/327

CPC classification number: G01N27/3272

Abstract: An electrochemical biosensor formed by screen printing and method of fabricating such biosensor is disclosed in the present invention. The biosensor can quickly absorb a sample to be measured therein, effectively control volume of the sample fed and “fill-and-position” the sample therein. The biosensor includes an electrode layer (electrode area) comprising two or three electrodes, which are a working electrode, a reference electrode and an auxiliary electrode (tri-electrode) on an insulating substrate. An active reaction layer containing reactant, reaction catalyst, mediator, wetting agent and surfactant is spread on the surface of the electrode layer. A sample inflow area is formed above the electrode area by adding an upper cover on top of a middle insulating layer with a U-shaped opening formed therein. Sample solution with a minute amount about 0.8 to 1 μl can be rapidly introduced into the electrode area and the active reaction layer via the inflow area by siphon or capillary, where the ingredient of the sample can be analysed by measuring reaction between the sample, reaction catalyst and mediator in the reaction layer using electrochemical potentiometric or amperometric method. An upwardly extended closed space formed within the upper cover above the electrode area adjacent to the front of conductive wires can be effectively used to control sample volume and “fill-and-position” the sample.

Abstract translation: 在本发明中公开了通过丝网印刷形成的电化学生物传感器和制造这种生物传感器的方法。 生物传感器可以快速吸收要测量的样品，有效地控制样品进料的体积并将样品“填充并定位”。 生物传感器包括在绝缘基板上包括作为工作电极，参考电极和辅助电极（三电极）的两个或三个电极的电极层（电极区域）。 含有反应物，反应催化剂，介体，润湿剂和表面活性剂的活性反应层扩散在电极层的表面上。 通过在其中形成有U形开口的中间绝缘层的顶部添加上盖，在电极区域的上方形成样品流入区域。 通过虹吸或毛细管可以通过虹吸或毛细管通过流入区域快速地将约0.8至1微摩尔的微量的样品溶液引入电极区域和活性反应层，其中样品的成分可以通过测量样品，反应 催化剂和介质在反应层中使用电化学电位或电流法。 可以有效地使用形成在电极区域之上的上电极区域内的向上延伸的封闭空间，该电极区域与导电线的前部相邻，以控制样品体积和“填充和定位”样品。

公开(公告)号：US07081192B1

公开(公告)日：2006-07-25

申请号：US09636104

申请日：2000-08-10

Applicant: Xiaobo Wang ,  Lei Wu ,  Jing Cheng ,  Weiping Yang ,  Junquan Xu

Inventor： Xiaobo Wang ,  Lei Wu ,  Jing Cheng ,  Weiping Yang ,  Junquan Xu

IPC: G01N27/447 ,  G01N33/48 ,  C12Q1/00

CPC classification number: G01N33/54386 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0439 ,  B01L2400/0454 ,  B01L2400/0496 ,  B03C5/028 ,  B03C2201/26 ,  G01N27/745 ,  G01N33/569

Abstract: This invention relates generally to the field of moiety or molecule manipulation in a chip format. In particular, the invention provides a method for manipulating a moiety in a microfluidic application, which method comprises: a) coupling a moiety to be manipulated onto surface of a binding partner of said moiety to form a moiety-binding partner complex; and b) manipulating said moiety-binding partner complex with a physical force in a chip format, wherein said manipulation is effected through a combination of a structure that is external to said chip and a structure that is built-in in said chip, thereby said moiety is manipulated.

公开(公告)号：US20060148102A1

公开(公告)日：2006-07-06

申请号：US10535905

申请日：2003-05-06

Applicant: Lianghong Guo ,  Dong Dong ,  Dong Zheng ,  Fuquan Wang ,  Xiqiang Yang ,  Jing Cheng

Inventor： Lianghong Guo ,  Dong Dong ,  Dong Zheng ,  Fuquan Wang ,  Xiqiang Yang ,  Jing Cheng

IPC: G01N33/551

CPC classification number: G01N33/582 ,  C12Q1/6816 ,  G01N2458/30 ,  C12Q2563/113 ,  C12Q2523/313

Abstract: The present invention provides apparatus, kits and methods for assaying analytes using photoelectrochemical molecules as labels. Specifically, the present invention provides a method for assaying an analyte, comprising: a) contacting a sample suspected of containing an analyte with a reactant capable of binding and/or reacting with the analyte under suitable conditions to allow binding of analyte, if present in the sample, to the reactant; and b) assessing binding and/or reacting between the analyte and the reactant to determine presence and/or amount of analyte in the sample, wherein the reactant, analyte, or additional reactant or additional analyte or analyte analog is labeled with a photoelectrochemically active molecule. The assessing step also comprises converting the photoelectrochemically active molecule with light to an excited state in the presence of an electrode, and assessing an electric current generated by an electron transfer between the excited photoelectrochemically active molecule and the electrode.

Abstract translation: 本发明提供了使用光电化学分子作为标记来测定分析物的装置，试剂盒和方法。 具体地，本发明提供了一种用于分析分析物的方法，其包括：a）在合适的条件下将疑似含有分析物的样品与能够与分析物结合和/或反应的反应物接触，以使分析物（如果存在于 样品，反应物; 和b）评估分析物和反应物之间的结合和/或反应以确定样品中分析物的存在和/或量，其中反应物，分析物或附加反应物或其他分析物或分析物类似物用光电化学活性分子标记 。 评估步骤还包括在电极存在下将光电化学活性分子与光转化为激发态，并评估由激发的光电化学活性分子和电极之间的电子转移产生的电流。

公开(公告)号：US20060141610A1

公开(公告)日：2006-06-29

申请号：US10546226

申请日：2003-03-28

Applicant: Wanli Xing ,  Fei Xian ,  Xianhua Wang ,  Jing Cheng

Inventor： Wanli Xing ,  Fei Xian ,  Xianhua Wang ,  Jing Cheng

IPC: C12M1/34

CPC classification number: C40B30/04 ,  B01J2219/00509 ,  B01J2219/00542 ,  B01J2219/00574 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01L3/50853 ,  B01L2300/0636 ,  B01L2300/0809 ,  B01L2300/0822 ,  B01L2400/0406 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12

Abstract: This invention relates generally to the field of microarray reaction devices and uses thereof. In particular, the invention a microarray reaction device wherein a plurality of reaction spaces are formed between a first and second plurality of projections, the heights of said plurality of reaction spaces being substantially identical and controllable by a supporting structure, and the relative positions between the first and second plurality of projections being controllable by a positioning structure. Articles of manufacture comprising the microarray reaction device and methods for assaying an analyte using the microarray reaction device are also provided.

Abstract translation: 本发明一般涉及微阵列反应装置的领域及其用途。 特别地，本发明是一种微阵列反应装置，其中在第一和第二多个突起之间形成多个反应空间，所述多个反应空间的高度基本上相同并且可由支撑结构控制，并且相对位置 第一和第二多个突起可由定位结构控制。 还提供了包含微阵列反应装置的制品和使用微阵列反应装置测定分析物的方法。

公开(公告)号：US20060141450A1

公开(公告)日：2006-06-29

申请号：US10538498

申请日：2002-12-31

Applicant: Xu Zhang ,  Xin Xie ,  Depu Chen ,  Weiyang Fei ,  Jing Cheng

Inventor： Xu Zhang ,  Xin Xie ,  Depu Chen ,  Weiyang Fei ,  Jing Cheng

IPC: C12Q1/70 ,  G01N33/567 ,  C12N1/20

CPC classification number: G01N33/54333 ,  C12N1/02 ,  C12N7/00 ,  C12N2760/16051 ,  C12N2795/14151 ,  C12Q1/24 ,  G01N33/54326

Abstract: This invention relates generally to the field of. In particular, the invention provides processes and kits for isolating a target cell, cellular organelle or virus from a sample, using inter alia, non- or low-specific binding between a target cell, cellular organelle or virus with a magnetic microbead.

Abstract translation: 本发明一般涉及领域。 特别地，本发明提供了用于使用靶细胞，细胞器官细胞或病毒与磁性微珠之间的非特异性或低特异性结合从样品中分离靶细胞，细胞器官细胞或病毒的方法和试剂盒。

公开(公告)号：US20060134608A1

公开(公告)日：2006-06-22

申请号：US10537378

申请日：2003-05-06

Applicant: Lianghong Guo ,  Yuanguang Li ,  Na Wang ,  Fuquan Wang ,  Xiqiang Yang ,  Jing Cheng

Inventor： Lianghong Guo ,  Yuanguang Li ,  Na Wang ,  Fuquan Wang ,  Xiqiang Yang ,  Jing Cheng

IPC: C12Q1/70 ,  C12Q1/68 ,  G01N33/567

CPC classification number: G01N33/5438 ,  C12Q1/6825 ,  G01N27/3277 ,  G01N33/58 ,  G01N2458/30

Abstract: This invention relates generally to the field of electrochemistry. In particular, the invention provides a method for assaying an analyte, using, inter alia, a reactant capable of binding and/or reacting with an analyte to be analyzed on an oxide electrode and a reducing agent not capable of being oxidized directly by said electrode to generate reduced electrochemically active molecule that participates in oxidation-reduction reactions repeatedly to generate an amplified electrochemical signal to determine presence and/or amount of said analyte in said sample.

Abstract translation: 本发明一般涉及电化学领域。 特别地，本发明提供了一种用于测定分析物的方法，特别是使用能够在不能被所述电极直接氧化的氧化物电极和还原剂上结合和/或与待分析的分析物反应的反应物 以产生重复参与氧化还原反应的还原电化学活性分子以产生放大的电化学信号以确定所述样品中所述分析物的存在和/或量。

公开(公告)号：US07030023B2

公开(公告)日：2006-04-18

申请号：US10655972

申请日：2003-09-04

Applicant: Shing-Chyang Pan ,  Ching-Hua Hsieh ,  Jing-Cheng Lin ,  Hsien-Ming Lee ,  Cheng-Lin Huang ,  Shau-Lin Shue

Inventor： Shing-Chyang Pan ,  Ching-Hua Hsieh ,  Jing-Cheng Lin ,  Hsien-Ming Lee ,  Cheng-Lin Huang ,  Shau-Lin Shue

IPC: H01L21/302

CPC classification number: H01L21/3105 ,  H01L21/28556 ,  H01L21/76807 ,  H01L21/76814 ,  H01L21/76838 ,  H01L21/76843 ,  H01L21/76873

Abstract: A method for forming a copper damascene feature including providing a semiconductor process wafer including at least one via opening formed to extend through a thickness of at least one dielectric insulating layer and an overlying trench line opening encompassing the at least one via opening to form a dual damascene opening; etching through an etch stop layer at the at least one via opening bottom portion to expose an underlying copper area; carrying out a sub-atmospheric DEGAS process with simultaneous heating of the process wafer in a hydrogen containing ambient; carrying out an in-situ sputter-clean process; and, forming a barrier layer in-situ to line the dual damascene opening.

Abstract translation: 一种用于形成铜镶嵌特征的方法，包括提供半导体工艺晶片，其包括形成为延伸穿过至少一个介电绝缘层的厚度的至少一个通孔开口，以及覆盖所述至少一个通孔开口的上覆沟槽开口，以形成双重 大马士革开幕 在所述至少一个通孔开口底部处蚀刻通过蚀刻停止层以暴露下面的铜区域; 在含氢环境中同时加热工艺晶片，进行亚低温DEGAS工艺; 进行原位溅射清洗过程; 并且原位形成阻挡层以使双镶嵌开口成线。

公开(公告)号：US06967155B2

公开(公告)日：2005-11-22

申请号：US10618536

申请日：2003-07-11

Applicant: Jing Cheng Lin ,  Ching-Hua Hsieh ,  Shau-Lin Shue ,  Mong-Song Liang

Inventor： Jing Cheng Lin ,  Ching-Hua Hsieh ,  Shau-Lin Shue ,  Mong-Song Liang

IPC: H01L21/44 ,  H01L21/4763 ,  H01L21/768 ,  H01L23/532 ,  H01L33/00

CPC classification number: H01L21/76843 ,  H01L21/76807 ,  H01L21/76831 ,  H01L21/76832 ,  H01L21/76834 ,  H01L21/76855 ,  H01L21/76864 ,  H01L21/76873 ,  H01L23/53238 ,  H01L23/53295 ,  H01L2924/0002 ,  H01L2924/00

Abstract: A new method and structure is provided for the creation of a copper dual damascene interconnect. A dual damascene structure is created in the layer of dielectric, optionally a metal barrier layer is deposited over exposed surfaces of the dual damascene structure. A copper seed layer is deposited, the dual damascene structure is filled with copper. An anneal is applied to the created copper interconnect after which excess copper is removed from the dielectric. Of critical importance to the invention, a thin layer of oxide is then deposited as a cap layer over the copper dual damascene interconnect, an etch stop layer is then deposited over the thin layer of oxide for continued upper-level metallization.

公开(公告)号：US06943111B2

公开(公告)日：2005-09-13

申请号：US10361732

申请日：2003-02-10

Applicant: Jing-Cheng Lin ,  Cheng-Lin Huang ,  Winston Shue ,  Mong-Song Liang

Inventor： Jing-Cheng Lin ,  Cheng-Lin Huang ,  Winston Shue ,  Mong-Song Liang

IPC: H01L21/768 ,  H01L21/4763 ,  H01L21/302 ,  H01L21/44

CPC classification number: H01L21/76843 ,  H01L21/76855 ,  H01L21/76864 ,  H01L21/76873 ,  H01L2221/1089

Abstract: A new method is provided for the creation of a copper seed interface capability. A first seed layer of copper alloy and a second seed layer of copper is provided over an opening in a layer of dielectric. The opening is filled with copper, the first and second seed layers are annealed.

Abstract translation: 提供了一种用于创建铜种子界面能力的新方法。 在介电层中的开口上方设置有铜合金的第一籽晶层和铜的第二晶种层。 开口填充有铜，第一和第二种子层被退火。