DILUTION SOLUTION FOR MEASURING FREE STEROIDS IN BLOOD AND METHOD FOR MEASURING STEROID USING THE SAME

    公开(公告)号:US20230314292A1

    公开(公告)日:2023-10-05

    申请号:US18078328

    申请日:2022-12-09

    CPC classification number: G01N1/38 G01N33/743 C01G9/06 C01D1/04 C01D7/00

    Abstract: The present invention relates to a diluted solution for measuring free steroids in blood and a method for measuring free steroids using the same, and more particularly, to a method for preparing a diluted blood sample for measuring free steroids in blood and a composition for blood sample dilution solution.
    In the method for preparing a diluted blood sample for measuring free steroids in blood of the present invention, it was confirmed that physiologically active free cortisol could be evaluated with the cortisol enzyme immunoreaction detection kit without additional sample pretreatment by removing cortisol which is present by binding to excess albumin and cortisol-specific corticosteroid-binding globulin (CBG) contained in serum using a protein precipitation and neutralizing agent. In addition, by selectively removing the protein in the blood sample, it is possible to measure low molecular weight compounds, including steroids, which are strongly bound to proteins in the blood, such as cortisol, in free form.
    Therefore, the method of the present invention can be applied simply and effectively to medical sites requiring blood tests for disease diagnosis, monitoring of free cortisol before and after surgery, and emergency patients.

    Method of Fabricating Block-Copolymer-based 3D Polymorphic Artificial Bilayer Membrane Structure

    公开(公告)号:US20230271179A1

    公开(公告)日:2023-08-31

    申请号:US17992084

    申请日:2022-11-22

    Abstract: The present invention relates to a method for manufacturing a three-dimensional structure based on a block copolymer. The method comprises the steps of injecting a block copolymer (BCP) solution into each micro-well formed on the substrate and drying it to form a block copolymer layer, and applying a buffer to the block copolymer layer to hydrate the micro-well in three dimensions Forming the structure, after the three-dimensional structure is formed, injecting and curing a hydrogel solution around the three-dimensional structure may include the step of enhancing stability. In particular, the process of hydration by applying a buffer to the micro-well is performed while an electric field is applied. By controlling the concentration of the block copolymer (BCP) and the amplitude and frequency of the electric field, a three-dimensional artificial cell membrane having a desired size and shape, such as a spherical or ciliary shape and high stability (100% survival for 50 days) is manufactured can do. The present invention can be efficiently applied to various biological fields such as artificial cells, cell-mimicking biosensors, and bioreactors.

    High efficiency and high sensitivity particle capture type terahertz sensing system

    公开(公告)号:US11703442B2

    公开(公告)日:2023-07-18

    申请号:US17138919

    申请日:2020-12-31

    CPC classification number: G01N15/1434 G01N15/1404 G01N2015/1415

    Abstract: Provided is a high efficiency and high sensitivity particle capture type terahertz sensing system. The particle capture type terahertz sensing system includes a sensing substrate to capture particles, and a terahertz sensor to emit terahertz electromagnetic waves to the sensing substrate to sense the particles, wherein the sensing substrate includes a base substrate and a particle capture structure layer formed on the base substrate, the particle capture structure layer includes a plurality of slits for focusing the terahertz electromagnetic waves, the particle capture structure layer captures the particles in the plurality of slits using dielectrophoresis, and an area in which the terahertz electromagnetic waves converge to the plurality of slits matches an area in which the particles are captured in the plurality of slits through the dielectrophoresis.

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