公开(公告)号：US07432342B2

公开(公告)日：2008-10-07

申请号：US10428254

申请日：2003-05-01

Applicant: Andreas Braun ,  Charles R. Cantor ,  Stefan M. Kammerer ,  Susan Taylor ,  Lora Burns-Hamuro ,  Charles Cook ,  Gary Olson ,  Christopher Self

Inventor： Andreas Braun ,  Charles R. Cantor ,  Stefan M. Kammerer ,  Susan Taylor ,  Lora Burns-Hamuro ,  Charles Cook ,  Gary Olson ,  Christopher Self

IPC: C07K7/08 ,  C07K14/00

CPC classification number: C07K14/47 ,  A01K67/0275 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/03

Abstract: A-kinase anchor protein (AKAPs) muteins, peptides thereof, and nucleic acids encoding the peptides are provided herein. Also provided are transgenic animals, cells comprising transgenes and various methods employing such peptides.

Abstract translation: 本文提供α-激酶锚蛋白（AKAP）突变蛋白，其肽，以及编码肽的核酸。 还提供了转基因动物，包含转基因的细胞和使用这种肽的各种方法。

公开(公告)号：US20080032287A1

公开(公告)日：2008-02-07

申请号：US10589709

申请日：2005-02-18

Applicant: Charles R. Cantor ,  Chunming Ding

Inventor： Charles R. Cantor ,  Chunming Ding

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2600/156 ,  C12Q2525/186 ,  C12Q2535/125 ,  C12Q2565/627 ,  C12Q2545/101

Abstract: The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s).

Abstract translation: 本发明涉及一种用于检测和定量核酸样品中罕见突变的方法。 正在研究的核酸分子可以是DNA或RNA。 罕见突变可以是任何类型的功能或非功能性核酸变化或突变，例如缺失，插入，易位，反转，一个或多个碱基取代或多态性。 因此，当目标是罕见的已知的与野生型或更常见的核酸变体混合的核酸变体时，本发明的方法可用于检测例如诊断，预后和随访应用中的罕见突变 （s）。

公开(公告)号：US07232688B2

公开(公告)日：2007-06-19

申请号：US09364774

申请日：1999-07-30

Applicant: Daniel P. Little ,  Maryanne J. O'Donnell-Maloney ,  Charles R. Cantor ,  Hubert Köster

Inventor： Daniel P. Little ,  Maryanne J. O'Donnell-Maloney ,  Charles R. Cantor ,  Hubert Köster

IPC: G01N24/00 ,  G01N1/10 ,  B01D59/44

CPC classification number: H01J49/0418 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00468 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00504 ,  B01J2219/00511 ,  B01J2219/0052 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/0268 ,  C07B2200/11 ,  C07F9/2408 ,  C07F9/2429 ,  C07H21/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N35/1067 ,  G01N35/1074 ,  G01N2035/1037 ,  G01N2035/1069 ,  Y10T428/26 ,  Y10T428/31678 ,  Y10T428/31855 ,  Y10T436/119163 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/2575

Abstract: Methods for dispensing tools that can be employed to generate multi-element arrays of sample material on a substrate surface. The resulting substrates are also provided. The substrates surfaces can be flat or geometrically altered to include wells of receiving material. The tool can dispense a spot of fluid to a substrate surface by spraying the fluid from the pin, contacting the substrate surface or forming a drop that touches against the substrate surface. The tool can form an array of sample material by dispensing sample material in a series of steps, while moving the pin to different locations above the substrate surface to form the sample array. The prepared sample arrays are passed to a plate assembly that disposes the sample arrays for analysis by mass spectrometry. To this end, a mass spectrometer is provided that generates a set of spectra signals that are indicative of the composition of the sample material under analysis.

Abstract translation: 用于分配可用于在基材表面上产生样品材料的多元素阵列的工具的方法。 还提供所得到的基底。 衬底表面可以是平坦的或几何上的改变以包括接收材料的孔。 该工具可以通过喷射来自销的流体，与基板表面接触或形成与基板表面接触的液滴来将流体点分配到基板表面。 该工具可以通过在一系列步骤中分配样品材料来形成样品材料阵列，同时将销移动到衬底表面上方的不同位置以形成样品阵列。 将制备的样品阵列传递到板组件，其通过质谱法分离样品阵列进行分析。 为此，提供了质谱仪，其生成指示待分析的样品材料的组成的一组光谱信号。

公开(公告)号：US6022951A

公开(公告)日：2000-02-08

申请号：US628540

申请日：1996-04-10

Applicant: Takeshi Sano ,  Charles R. Cantor ,  Sandor Vajda ,  Gabriel O. Reznik ,  Cassandra L. Smith ,  Mark W. Pandori

Inventor： Takeshi Sano ,  Charles R. Cantor ,  Sandor Vajda ,  Gabriel O. Reznik ,  Cassandra L. Smith ,  Mark W. Pandori

IPC: A61K38/00 ,  C07K14/36 ,  C12N1/21 ,  C12N15/31 ,  G01N33/538

CPC classification number: G01N33/538 ,  C07K14/36 ,  A61K38/00 ,  G01N2333/465 ,  Y10S530/808 ,  Y10S530/81

Abstract: The present invention relates to streptavidin proteins and peptides having a altered physical properties such as an increased stability or increased or decreased affinity for binding biotin. The invention also relates to methods for the detection, identification, separation and isolation of targets using streptavidin proteins or peptides. Streptavidin with increased or reduced affinity allows for the use of the streptavidin-biotin coupling systems for detection and isolation systems wherein it is necessary to remove of one or the other of the binding partners. Such systems are useful for the purification of functional proteins and viable cells. The invention also relates to nucleic acids which encode these streptavidin proteins and peptides and to recombinant cells such as bacteria, yeast and mammalian cells which contain these nucleic acids.

Abstract translation: 本发明涉及链霉抗生物素蛋白和具有改变的物理性质例如增加的稳定性或增加或降低的结合生物素的亲和力的肽。 本发明还涉及使用链霉亲和素蛋白或肽检测，鉴定，分离和分离靶的方法。 具有增加或降低亲和力的链霉亲和素允许使用链霉抗生物素蛋白 - 生物素偶联系统进行检测和分离系统，其中必须除去一种或另一种结合配偶体。 这样的系统可用于纯化功能性蛋白质和活细胞。 本发明还涉及编码这些链霉抗生物素蛋白和肽以及含有这些核酸的重组细胞如细菌，酵母和哺乳动物细胞的核酸。

公开(公告)号：US6010849A

公开(公告)日：2000-01-04

申请号：US482080

申请日：1995-06-07

Applicant: Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin ,  Kirk E. Fry

Inventor： Cynthia A. Edwards ,  Charles R. Cantor ,  Beth M. Andrews ,  Lisa M. Turin ,  Kirk E. Fry

IPC: C07K14/47 ,  C12N15/10 ,  C12N15/67 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/53

CPC classification number: C12Q1/701 ,  C07K14/47 ,  C12N15/1048 ,  C12N15/67 ,  C12Q1/6811 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q1/705 ,  H01L2924/14

Abstract: The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.

Abstract translation: 本发明定义了一种DNA：蛋白结合测定法，用于筛选合成或生物化合物文库结合DNA测试序列的能力。 该测定法是通用的，因为可以通过将测试序列置于与定义的蛋白质结合筛选序列相邻的位置来测试任何数量的测试序列。 分子与这些测试序列的结合改变了蛋白质分子与其同源结合序列的结合特征。 当这样的分子结合测试序列时，DNA：蛋白复合物的平衡受到干扰，产生游离DNA探针浓度的变化。 阐述了许多示例性目标测试序列（SEQ ID NO：1至SEQ ID NO：600）。 本发明的测定也可用于表征任何所选DNA结合分子的优选结合序列。

公开(公告)号：US6007987A

公开(公告)日：1999-12-28

申请号：US730185

申请日：1996-10-15

Applicant: Charles R. Cantor ,  Marek Przetakiewicz ,  Takeshi Sano ,  Cassandra L. Smith

Inventor： Charles R. Cantor ,  Marek Przetakiewicz ,  Takeshi Sano ,  Cassandra L. Smith

IPC: C12Q1/68 ,  C40B40/06

CPC classification number: C12Q1/6837 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06

Abstract: This invention is directed to methods and reagents useful for sequencing nucleic acid targets utilizing sequencing by hybridization technology comprising probes, arrays of probes and methods whereby sequence information is obtained rapidly and efficiently in discrete packages. That information can be used for the detection, identification, purification and complete or partial sequencing of a particular target nucleic acid. When coupled with a ligation step, these methods can be performed under a single set of hybridization conditions. The invention also relates to the replication of probe arrays and methods for making and replicating arrays of probes which are useful for the large scale manufacture of diagnostic aids used to screen biological samples for specific target sequences. Arrays created using PCR technology may comprise probes with 5'- and/or 3'-overhangs.

Abstract translation: 本发明涉及可用于使用包括探针，探针阵列和方法的杂交技术测​​序的用于测序核酸靶的方法和试剂，从而在离散包装中快速有效地获得序列信息。 该信息可以用于特定靶核酸的检测，鉴定，纯化和完全或部分测序。 当与连接步骤相结合时，这些方法可以在单一杂交条件下进行。 本发明还涉及探针阵列的复制和用于制备和复制探针阵列的方法，所述探针阵列可用于大规模制造用于筛选特定靶序列的生物样品的诊断辅助物。 使用PCR技术产生的阵列可以包含具有5'和/或3'突出端的探针。

公开(公告)号：US5744131A

公开(公告)日：1998-04-28

申请号：US476876

申请日：1995-06-07

Applicant: Cynthia A. Edwards ,  Kirk E. Fry ,  Charles R. Cantor ,  Beth M. Andrews

Inventor： Cynthia A. Edwards ,  Kirk E. Fry ,  Charles R. Cantor ,  Beth M. Andrews

IPC: C07K14/47 ,  C12N15/09 ,  C12N15/10 ,  C12N15/67 ,  C12Q1/68 ,  C12Q1/70 ,  A61K31/74 ,  G01N33/558 ,  G01N33/566

CPC classification number: C12N15/67 ,  C07K14/47 ,  C12N15/1048 ,  C12Q1/6811 ,  C12Q1/6883 ,  C12Q1/705

Abstract: The present invention defines an assay useful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA test sequences. The assay is also useful for determining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. Also described herein are potential applications of the assay, including: 1) the detection of lead compounds or new drugs via the mass screening of libraries of synthetic or biological compounds (i.e., fermentation broths); 2) the design of sequence-specific DNA-binding drugs comprised of homo- or hetero-meric subunits of molecules for which the sequence specificity was determined using the assay; and 3) the use of molecules for which sequence specificity was determined using the assay as covalently attached moieties to aid in the binding of nucleic acid or other macromolecular polymers to nucleic acid sequences.

公开(公告)号：US4908308A

公开(公告)日：1990-03-13

申请号：US746282

申请日：1985-06-19

Applicant: Lex H. T. Van der Ploeg ,  Suzanne H. Giannini ,  Charles R. Cantor

Inventor： Lex H. T. Van der Ploeg ,  Suzanne H. Giannini ,  Charles R. Cantor

IPC: C12Q1/68

CPC classification number: C12Q1/6893 ,  C12Q2600/158

Abstract: This invention concerns a method for identifying in vitro an animal-infective form of a parasitic protozoan which comprises recovering total mRNA from the protozoan and detecting in the mRNA so recovered the presence of a mRNA transcript encoding a heat shock protein associated with animal-infective parasitic protozoans, which transcript is present only in the animal-infective form of the protozoan, or quantitatively determining in the mRNA so recovered the number of a mRNA transcript encoding a heat shock protein associated with animal-infective parasitic protozoans, which transcript is present in increased number only in the animal-infective form of the parasitic protozoan.This invention also concerns a method for identifying an agent capable of blocking the formation of the animal-infective form of a parasitic protozoan.

Abstract translation: 本发明涉及用于体外鉴定寄生原生动物的动物感染形式的方法，其包括从原生动物中回收总mRNA并检测mRNA，从而回收编码与动物感染性寄生虫有关的热休克蛋白的mRNA转录物的存在 原生动物，其转录物仅存在于原生动物的动物感染形式中，或者在mRNA中定量测定，从而回收编码与动物感染性寄生原生动物相关的热休克蛋白的mRNA转录物的数目，该转录物存在于增加的 数量仅在寄生原生动物的动物感染形式中。 本发明还涉及鉴定能够阻断寄生原生动物的动物感染形式形成的试剂的方法。

公开(公告)号：US4473452A

公开(公告)日：1984-09-25

申请号：US442580

申请日：1982-11-18

Applicant: Charles R. Cantor ,  David C. Schwartz

Inventor： Charles R. Cantor ,  David C. Schwartz

IPC: G01N27/26 ,  B01D20060101 ,  B01D57/02 ,  G01N20060101 ,  G01N27/447 ,  G01N27/28

CPC classification number: G01N27/44773

Abstract: Disclosed are an apparatus for and a method of electrophoretically separating particles by electric fields which are transverse to each other, which alternate between respective high and low intensities out of phase with each other at a frequency related to the mass of the particles and which move the particles in an overall direction transverse to the respective directions of the fields. For separating large macromolecules, at least one of the fields preferably has an intensity gradient in a direction transverse to its own. The new arrangement makes it possible to: (1) separate particles (molecules) larger in size than those able to be separated with previously known techniques, (2) carry out separation at higher speed and at better resolution than is possible with previously known techniques, and (3) concurrently separate particles which differ greatly in mass (molecular weight).

Abstract translation: 公开了一种通过电场电泳分离颗粒的装置和方法，它们彼此横向，它们以相对于颗粒的质量的频率彼此相位的相应的高和低强度之间交替， 颗粒在横向于各个方向的整个方向上。 为了分离大的大分子，至少一个场优选在横向于其自身的方向上具有强度梯度。 新的布置使得可以：（1）分离比以前已知技术更大尺寸的颗粒（分子），（2）以比先前已知技术更高的速度和更好的分辨率进行分离 ，（3）同时分离质量差异很大的分子（分子量）。