公开(公告)号：US06837992B2

公开(公告)日：2005-01-04

申请号：US10355323

申请日：2003-01-31

申请人： Yuri Gerner ,  Carl W. Sims ,  Thomas Thielen

发明人： Yuri Gerner ,  Carl W. Sims ,  Thomas Thielen

IPC分类号： B01D61/00 ,  B01D19/00 ,  B01D71/36 ,  B01F5/00 ,  G01N30/02 ,  G01N30/26 ,  G01N30/32 ,  G01N30/34 ,  G01N30/36 ,  B01D15/08

CPC分类号： G01N30/32 ,  B01D19/0031 ,  G01N30/34 ,  G01N30/36 ,  G01N2030/027 ,  G01N2030/322

摘要： An integrated mobile phase degassing and blending apparatus for transport of liquid chromatography fluids therethrough includes a component having a first portion and a second portion, with the first portion defining an enclosed degassing chamber, and the second portion including a mobile phase blending device, wherein the degassing chamber and the mobile phase blending device are operably coupled to one another such that the liquid chromatography fluids pass directly into the blending device from the degassing chamber. In a particular embodiment, the degassing chamber is specifically configured to accommodate multiple distinct flexible degassing tubes.

摘要翻译： 用于运输液相色谱流体的整合流动相脱气和混合装置包括具有第一部分和第二部分的部件，其中第一部分限定封闭的脱气室，第二部分包括流动相混合装置，其中 脱气室和流动相共混装置可操作地彼此耦合，使得液相色谱流体从脱气室直接进入共混装置。 在特定实施例中，脱气室被特别地构造成容纳多个不同的柔性脱气管。

公开(公告)号：US5935443A

公开(公告)日：1999-08-10

申请号：US818064

申请日：1997-03-14

申请人： James M. Anderson, Jr. ,  Raaidah Saari-Nordhaus ,  Carl W. Sims ,  Yuri E. Gerner

发明人： James M. Anderson, Jr. ,  Raaidah Saari-Nordhaus ,  Carl W. Sims ,  Yuri E. Gerner

IPC分类号： G01N27/06 ,  B01D15/04 ,  B01D15/08 ,  B01J39/26 ,  B01J41/20 ,  B01J49/00 ,  G01N30/02 ,  G01N30/08 ,  G01N30/14 ,  G01N30/26 ,  G01N30/28 ,  G01N30/34 ,  G01N30/46 ,  G01N30/52 ,  G01N30/60 ,  G01N30/96

CPC分类号： G01N30/52 ,  B01D15/203 ,  B01D15/361 ,  B01J39/26 ,  G01N30/96 ,  G01N2030/285 ,  G01N2030/965 ,  G01N30/02 ,  G01N30/34 ,  G01N30/468

摘要： A system and method for electrochemically regenerated ion neutralization and concentration is disclosed. In one aspect of the invention, a system is provided comprising a HPLC column pump, a concentrator column, an analytical column, a suppressor, a detector, a mixed bed deionizing resin, a sample injector, and a neutralization cartridge.

摘要翻译： 公开了一种用于电化学再生离子中和和浓缩的系统和方法。 在本发明的一个方面，提供一种包括HPLC柱泵，浓缩塔，分析柱，抑制器，检测器，混合床去离子树脂，样品注射器和中和盒的系统。

公开(公告)号：US5788845A

公开(公告)日：1998-08-04

申请号：US872329

申请日：1997-06-10

申请人： Jan Olof Jeppsson

发明人： Jan Olof Jeppsson

IPC分类号： C07K14/79 ,  G01N30/02 ,  G01N30/06 ,  G01N30/14 ,  G01N30/26 ,  G01N30/34 ,  G01N30/74 ,  G01N30/88 ,  G01N33/68 ,  G01N33/98 ,  B01D15/08

CPC分类号： G01N33/68 ,  C07K14/79 ,  G01N2030/027 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N2333/79 ,  Y10T436/204165

摘要： A method for determining the amount of carbohydrate deficient transferrin (CDT) in blood serum involves saturating a sample of the blood serum with iron to provide isoforms of transferrin, separating the isoforms of transferrin from other serum proteins in the sample, and developing a chromatogram which relates the separated isoforms with different pI values. The separation step is preferably carried out in an ion exchange column using a salt gradient and the chromatogram is preferably developed using a high performance liquid chromatograph (HPLC). The method is particularly useful for identifying heavy alcohol consumption in a person, which is related to isoforms of transferrin having pI values of 5.9 and 5.7.

摘要翻译： 用于测定血清中碳水化合物缺乏型转铁蛋​​白（CDT）量的方法包括用铁饱和血清样品以提供转铁蛋白的同种型，将转铁蛋白的同种型与样品中的其他血清蛋白分离，并开发色谱图 将分离的异构体与不同的pI值相关联。 分离步骤优选使用盐梯度在离子交换柱中进行，并且色谱图优选使用高效液相色谱（HPLC）显影。 该方法特别可用于鉴定人中的重度酒精消耗，其与pI值为5.9和5.7的转铁蛋白的同种型相关。

公开(公告)号：US5506103A

公开(公告)日：1996-04-09

申请号：US153365

申请日：1993-11-16

申请人： Aharon S. Cohen ,  Andre Bourque

发明人： Aharon S. Cohen ,  Andre Bourque

IPC分类号： G01N33/50 ,  C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  G01N30/00 ,  G01N30/26 ,  G01N30/34 ,  G01N30/88 ,  C07H21/00 ,  G01N33/53

CPC分类号： C12Q1/68 ,  C12N15/101

摘要： Disclosed is a method for detecting and quantitating oligonucleotides with charged internucleotide linkages in biological fluids. In this method, a biological fluid sample is contacted with an anion exchange resin at from 40.degree. C. to 65.degree. C. for a time sufficient to enable oligonucleotides in the sample to adsorb to the resin. The absorbed oligonucleotides are then desorbed with a buffer having a salt concentration of about 1 M to 2.5 M and a pH in the range of about 6.5 to 7.5, the desorption being performed at about 40.degree.-65.degree. C. The oligonucleotides so released are then detected and quantitated.

摘要翻译： 公开了用于检测和定量生物流体中带有核苷酸间键的寡核苷酸的方法。 在该方法中，生物流体样品在40℃至65℃下与阴离子交换树脂接触足够长的时间以使得样品中的寡核苷酸能够吸附到树脂上。 然后将吸收的寡核苷酸用盐浓度为约1M至2.5M，pH为约6.5至7.5的缓冲液解吸，解吸在约40-65℃进行。如此释放的寡核苷酸是 然后检测并定量。

公开(公告)号：US5288113A

公开(公告)日：1994-02-22

申请号：US996697

申请日：1992-12-24

申请人： Paul H. Silvis ,  Bradley R. Rightnour ,  Richard A. Morehead

发明人： Paul H. Silvis ,  Bradley R. Rightnour ,  Richard A. Morehead

IPC分类号： G01N30/26 ,  F16L19/06 ,  G01N30/60 ,  F16L25/00 ,  F16L35/00

CPC分类号： G01N30/6034 ,  G01N30/6026 ,  Y10S285/911

摘要： An easily-assembled, heat-resistant connector for releasably joining end portions of two capillary tubes in end-to-end fashion for use in chromatography without interrupting fluid flow or interfering with chromatographic results, comprises a cylindrical sleeve which has a bore which tapers toward a center point to form press-fit seals with the tips of two capillary tubes. A first ferrule is mounted on the end portion of a first capillary tube, and a second ferrule is mounted on the end portion of a second capillary tube. A jacket surrounds and contains the sleeve, and a first adjustment screw mounted on the jacket pushes against the first ferrule to deform the ferrule into sealing contact with the outside surface of the first capillary tube and with the inside surface of the sleeve. A second adjustment screw mounted on the jacket pushes against the second ferrule to deform the ferrule into sealing contact with the outside surface of the second capillary tube and with the inside surface of the sleeve. The pressure on the first ferrule may be adjusted individually and independently of the pressure on the second ferrule and the pressure on the second ferrule, may be adjusted individually and independently of the pressure on the first ferrule. The pressure from the ferrules also hold the press-fit seals in sealing contact.

摘要翻译： 一种易于组装的耐热连接器，用于以端对端的方式可拆卸地连接两根毛细管的端部，用于色谱法而不中断流体流动或干扰色谱结果，包括圆柱形套筒，其具有朝向 一个中心点与两个毛细管的尖端形成压配合密封。 第一套圈安装在第一毛细管的端部上，第二套圈安装在第二毛细管的端部上。 护套围绕并包含套筒，并且安装在护套上的第一调节螺钉推压第一套圈以使套圈变形成与第一毛细管的外表面和套筒的内表面密封接触。 安装在护套上的第二调节螺钉推动第二套圈，使套圈变形成与第二毛细管的外表面和套筒的内表面密封接触。 第一套圈上的压力可以单独地调节并且独立于第二套圈上的压力和第二套圈上的压力，可以单独地调节并独立于第一套圈上的压力来调节。 套圈的压力也使压配密封件保持密封接触。

公开(公告)号：US5224510A

公开(公告)日：1993-07-06

申请号：US606405

申请日：1990-10-31

申请人： Nico Pericles

发明人： Nico Pericles

IPC分类号： G01N30/02 ,  F16K31/00 ,  F16K31/02 ,  G01N30/20 ,  G01N30/26 ,  G01N30/30 ,  G01N30/32 ,  G01N30/36

CPC分类号： G01N30/20 ,  F16K31/004 ,  G01N2030/3038 ,  G01N2030/328 ,  G01N30/02 ,  G01N30/32 ,  G01N30/36 ,  Y10T137/6606 ,  Y10T137/7761

摘要： A high-pressure control valve (2) used in chromatography serves as a counter pressure control valve which, in a separating column, is intended to maintain a specific predeterminable pressure. The valve has a valve seat (12) with an outlet channel opening (18) located in its center and a valve body (13) that cooperates with the valve seat. The valve body (13) can be moved with the aid of a piezo transducer, actively in the opening direction and, while being monitored, actively in the closing direction. The opposed faces of the valve seat (12) and the valve body (13) are so shaped that an annular gap (16) that widens radially towards the outside starting from the opening (18) of the outlet channel is produced. When the valve is in the closed position, this results in the formation of a narrow sealing zone directly at the edge of the opening of the outlet channel.

摘要翻译： 用于色谱的高压控制阀（2）用作反压控制阀，其在分离柱中旨在保持特定的可预定压力。 阀具有阀座（12），其中位于其中心的出口通道开口（18）和与阀座配合的阀体（13）。 阀体（13）可以借助于压电换能器主动地沿打开方向移动，并且在关闭方向上被主动地被监控。 阀座（12）和阀体（13）的相对面被成形为使得从出口通道的开口（18）径向向外径向扩大的环形间隙（16）。 当阀处于关闭位置时，这导致在出口通道的开口的边缘处直接形成窄的密封区。

公开(公告)号：US4988446A

公开(公告)日：1991-01-29

申请号：US358779

申请日：1989-05-30

申请人： Joel I. Haberman ,  Robert E. Overfield ,  Winston K. Robbins

发明人： Joel I. Haberman ,  Robert E. Overfield ,  Winston K. Robbins

IPC分类号： G01N21/33 ,  C10G11/18 ,  C10G21/30 ,  C10G69/04 ,  G01N30/26 ,  G01N30/34 ,  G01N30/46 ,  G01N30/74 ,  G01N30/86 ,  G01N30/88 ,  G01N33/28 ,  B01D15/08

CPC分类号： G01N30/461 ,  C10G11/187 ,  C10G21/30 ,  C10G69/04 ,  G01N30/74 ,  G01N30/8675 ,  G01N33/2829 ,  G01N2030/484 ,  G01N2030/8854 ,  G01N21/33 ,  G01N30/34 ,  Y10T436/212 ,  Y10T436/218

摘要： A sample of a hydrocarbon oil containing asphaltenes is chromatographically analyzed by forming a mixture of the oil with a weak solvent. The mixture is passed in contact with a column of a stationary phase of fine solid particles of fully functionalized material, followed by a weak solvent. The solvent, after recovery from the column, is analyzed for aromatics by UV-absorption of UV radiation in the range 200 to 400 nm. The absorbance of the UV light by the irradiated eluents across the UV wavelength range is monitored and the integral of absorbance is derived as a function of photon energy across the wavelength range. The magnitude of the derived integral in at least one time interval corresponding with aromatics in the eluent from the stationary phase is measured as an indication of the level of aromatics in the oil sample. The weak solvent may be followed by a strong solvent which, in turn, may be followed by a strong solvent which is modified by the addition of a hydrogen bonding solvent.

摘要翻译： 通过形成油与弱溶剂的混合物对含有沥青质的烃油样品进行色谱分析。 使混合物与完全官能化材料的细固体颗粒的固定相的柱接触，然后与弱溶剂接触。 通过紫外线吸收200至400nm范围内的UV辐射，从柱中回收溶剂后，分析芳族化合物。 通过紫外线波长范围内的照射洗脱液对紫外光的吸光度进行监测，并将吸收光积分作为光子能量的函数导出。 测量在与固定相的洗脱液中的芳族化合物相应的至少一个时间间隔内的导出积分的量值作为油样品中芳族化合物水平的指示。 弱溶剂之后可以是强溶剂，然后可以继续加入通过加入氢键溶剂进行改性的强溶剂。

公开(公告)号：US4960516A

公开(公告)日：1990-10-02

申请号：US328034

申请日：1989-01-23

申请人： Maxim L. Alexandrov ,  Boris G. Belenky ,  Vladimir A. Gotlib ,  Arkady P. Vlasov ,  Nikolai N. Komarov ,  Valery V. Sudiiin ,  Vsevolod V. Shevkunov

发明人： Maxim L. Alexandrov ,  Boris G. Belenky ,  Vladimir A. Gotlib ,  Arkady P. Vlasov ,  Nikolai N. Komarov ,  Valery V. Sudiiin ,  Vsevolod V. Shevkunov

IPC分类号： B01D15/08 ,  G01N30/26 ,  G01N30/32 ,  G01N30/34 ,  G01N30/88

CPC分类号： G01N30/88 ,  G01N30/26 ,  G01N30/34

摘要： The liquid microcolumn chromatograph comprises a microcolumn (1), unit (2) for feeding eluent, made as a reservoir (3) separated by a "slack" diaphragm (4) into two half-chambers (5,6) one whereof communicates with a source (7) of pressure and the other is connected to a source (8) of eluent and to an inlet into the microcolumn (1) a flow-through cell (9), and an eluent-collecting vessel (14). The chromatograph is provided with an additional reservoir (10) separated by a "slack" diaphragm (11) into two half-chambers (12, 13) and is also provided with an additional source (15) of pressure, the half-chamber (12) being communicated with an outlet of the flow-through cell (9) and connected to the eluent-collecting vessel (14), whereas the half-chamber (13) communicates with the additional source (15) of pressure.

摘要翻译： PCT No.PCT / SU87 / 00068 Sec。 371日期1989年1月23日 102（e）日期1989年1月23日PCT提交1987年6月16日PCT公布。 出版物WO88 / 10425 日期为1988年12月29日。液体微柱色谱仪包括微柱（1），用于进料洗脱液的单元（2），作为由“松弛”隔膜（4）分隔成两个半室（5）的储存器（3） ，6）其中一个与压力源（7）连通，另一个连接到洗脱液源（8），并连接到微柱（1）的入口，流通池（9） 收集容器（14）。 色谱仪设置有由“松弛”隔膜（11）分隔成两个半室（12,13）的另外的储存器（10），并且还设置有另外的压力源（15），半室（ 12）与流通池（9）的出口连通并连接到洗脱液收集容器（14），而半室（13）与附加的压力源（15）连通。

公开(公告)号：US4818687A

公开(公告)日：1989-04-04

申请号：US667069

申请日：1985-02-28

申请人： John D. Groopman ,  Gerald N. Wogan

发明人： John D. Groopman ,  Gerald N. Wogan

IPC分类号： G01N33/53 ,  B01J20/281 ,  C07K16/14 ,  C07K16/44 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  G01N30/26 ,  G01N30/88 ,  G01N33/543 ,  G01N33/548 ,  G01N33/577

CPC分类号： C07K16/44 ,  C07K16/14 ,  G01N33/543 ,  Y10S436/815 ,  Y10S436/822 ,  Y10S436/824 ,  Y10S436/825

摘要： An affinity matrix and a method for the detection of low molecular weight compositions such as aflatoxins are provided utilizing specific monoclonal IgM antibody having an affinity constant not less than about 1.times.10.sup.9 liters per mole. Methods for the preparation and use of such affinity matrices are also given. The detection is rapid, accurate, reproducible, and allows for quantitative recovery of the composition of interest.

摘要翻译： 使用亲和常数不小于约1×10 9升/摩尔的特异性单克隆IgM抗体提供亲和基质和检测低分子量组合物如黄曲霉毒素的方法。 还给出了制备和使用这种亲和基质的方法。 检测是快速，准确，可重复的，并且允许定量回收所关注的组合物。

公开(公告)号：US4706700A

公开(公告)日：1987-11-17

申请号：US907932

申请日：1986-09-16

申请人： Elizabeth J. Jumeau

发明人： Elizabeth J. Jumeau

IPC分类号： G01N27/62 ,  A61B5/145 ,  G01N1/00 ,  G01N30/20 ,  G01N30/26 ,  H01J49/04 ,  F16K13/10

CPC分类号： G01N35/1097 ,  G01N30/20 ,  H01J49/0495 ,  Y10T137/4531 ,  Y10T137/87571

摘要： A valve arrangement for controlling the flow of a sample of blood gases along a capillaric streamway from a probe towards an analyzing apparatus includes a body 12 having an inlet 36 for control fluid and a reciprocable valve head 20 movable from a first position in which inlet 36 is isolated from a second inlet 30 for carrier gas and blood gases towards a second position in which inlets 36, 30 are in communication. The arrangement is such, that in the first position of the valve head 20 the carrier gas and blood gases can flow from inlet 30 towards an outlet 32. However, in the second position the pressure of the control fluid prevents the flow of the carrier gas and blood gases through the inlet 30.

摘要翻译： 用于控制沿着毛细管流的血气样品从探针朝向分析装置的流动的阀装置包括具有用于控制流体的入口36的主体12和可从第一位置移动的往复式阀头20，其中入口36 从用于载体气体和血液气体的第二入口30隔离入入口36,30连通的第二位置。 这样做的结构是，在阀头20的第一位置，载气和血气可以从入口30流向出口32.然而，在第二位置，控制流体的压力防止了载气的流动 和血气通过入口30。